Reviewed, UniProtKB/Swiss-Prot Q487R3 (DSBD_COLP3)

Names and origin

Protein names

Recommended name:
    Thiol:disulfide interchange protein dsbD
    EC=1.8.1.8
Alternative name(s):
    Protein-disulfide reductase
      Short name=Disulfide reductase

Gene names

Name:	dsbD
Ordered Locus Names:	CPS_0953

Organism

Colwellia psychrerythraea (strain 34H / ATCC BAA-681) (Vibrio psychroerythus) [Complete proteome] [HAMAP]

Taxonomic identifier

167879 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Colwelliaceae › Colwellia

Protein attributes

Sequence length	608 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Required to facilitate the formation of correct disulfide bonds in some periplasmic proteins and for the assembly of the periplasmic c-type cytochromes. Acts by transferring electrons from cytoplasmic thioredoxin to the periplasm. This transfer involves a cascade of disulfide bond formation and reduction steps By similarity.
Catalytic activity	Protein dithiol + NAD(P)+ = protein disulfide + NAD(P)H. HAMAP MF_00399
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the thioredoxin family. DsbD subfamily. Contains 1 thioredoxin domain.

Ontologies

Keywords
Biological process	Cytochrome c-type biogenesis Electron transport Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Redox-active center Signal Transmembrane
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Disulfide bond
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro cytochrome complex assembly Inferred from electronic annotation. Source: HAMAP electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	electron carrier activity Inferred from electronic annotation. Source: HAMAP protein-disulfide reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 608	586	Thiol:disulfide interchange protein dsbD HAMAP MF_00399		PRO_0000304384
Regions
	Transmembrane	194 – 214	21	Potential
	Transmembrane	241 – 261	21	Potential
	Transmembrane	273 – 293	21	Potential
	Transmembrane	314 – 334	21	Potential
	Transmembrane	352 – 372	21	Potential
	Transmembrane	387 – 407	21	Potential
	Transmembrane	414 – 434	21	Potential
	Transmembrane	456 – 476	21	Potential
	Domain	469 – 608	140	Thioredoxin
Amino acid modifications
	Disulfide bond	135 ↔ 141		Redox-active By similarity
	Disulfide bond	212 ↔ 334		Redox-active By similarity
	Disulfide bond	522 ↔ 525		Redox-active By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q487R3-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 61FA123A713FA0B7	FASTA	608	66,804
	10 20 30 40 50 60 MKNLLSLCFL MLAAFTLNPA AAQEKQSIFD VSNSSLFSND DEFLKVDQAF AFNFYQKNNL 70 80 90 100 110 120 LEVSFDIAPE YYLYRHQFKF KGKNTQFTSV LLPDGIDHED EFFGVQKIFT ENLAFTVNLE 130 140 150 160 170 180 NVSNDASIKI TYQGCAEKGL CYPPTSKVIK LSKFILGEST SAPSSDAAQQ TNEGEVKKSE 190 200 210 220 230 240 QHQLSDMLKQ DSLLLTLIAF FVGGLLLSFT PCVFPMYPIL TGIIVGQGEG LTTKKAFTLS 250 260 270 280 290 300 FFYVQGMAIT YTLLGVVVAM AGAKFQAVFQ HPIVLIGLSI LFIFLALSMF GVFNLALPAS 310 320 330 340 350 360 WQNKLNNVSN KQKGGSITGV LMMGVISGLV ASPCTTAPLT GALLYISQTG DVVLGASALY 370 380 390 400 410 420 ALSLGMGLPL LILGSSGGKL LPKAGAWMNI IKNIFGLLLL AVPVFLLERF IPEVASQALW 430 440 450 460 470 480 ALLILVSASY FYVANQNHAA AQNVQQGKGF WYGLRSLVIF LMLFFGANLA YQLIYPSSNN 490 500 510 520 530 540 VTNNAQHASF KQVTSLAQLE DEVKKANMQG KTVMVDLYAD WCIACKEFEK YTFVDADVQK 550 560 570 580 590 600 ALSNSVWLQI DMTEFDSTDN AELVQHYTIL GLPSILFFDL QGNELTKQRT TGFMKAAEFS AHVKSIFK

References

[1]

"The psychrophilic lifestyle as revealed by the genome sequence of Colwellia psychrerythraea 34H through genomic and proteomic analyses." Methe B.A., Nelson K.E., Deming J.W., Momen B., Melamud E., Zhang X., Moult J., Madupu R., Nelson W.C., Dodson R.J., Brinkac L.M., Daugherty S.C., Durkin A.S., DeBoy R.T., Kolonay J.F., Sullivan S.A., Zhou L., Davidsen T.M. , Wu M., Huston A.L., Lewis M., Weaver B., Weidman J.F., Khouri H., Utterback T.R., Feldblyum T.V., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 102:10913-10918(2005) [PubMed: 16043709] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000083 Genomic DNA. Translation: AAZ24775.1.
RefSeq	YP_267702.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3518818.
GenomeReviews	Gene locus CPS_0953 in contig CP000083_GR.
KEGG	cps:CPS_0953.
NMPDR	fig|167879.3.peg.920.
TIGR	CPS_0953.
Phylogenomic databases
HOGENOM	Q487R3.
OMA	Q487R3. TITHILW.
Enzyme and pathway databases
BioCyc	CPSY167879:CPS_0953-MON.
Family and domain databases
HAMAP	MF_00399. [Tree]
InterPro	IPR003834. Cyt_c_assmbl_TM. IPR017936. Thioredoxin-like. IPR017937. Thioredoxin_CS. IPR013766. Thioredoxin_domain. IPR012335. Thioredoxin_fold. [Graphical view]
Gene3D	G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
Pfam	PF02683. DsbD. 1 hit. PF00085. Thioredoxin. 1 hit. [Graphical view]
PROSITE	PS00194. THIOREDOXIN_1. False negative. PS51352. THIOREDOXIN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DSBD_COLP3

Accession

Primary (citable) accession number: Q487R3

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	September 13, 2005
Last modified:	June 16, 2009
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)