Reviewed, UniProtKB/Swiss-Prot Q487G4 (NAPA_COLP3)

Names and origin

Protein names

Recommended name:
    Periplasmic nitrate reductase
    EC=1.7.99.4

Gene names

Name:	napA
Ordered Locus Names:	CPS_1057

Organism

Colwellia psychrerythraea (strain 34H / ATCC BAA-681) (Vibrio psychroerythus) [Complete proteome] [HAMAP]

Taxonomic identifier

167879 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Colwelliaceae › Colwellia

Protein attributes

Sequence length	832 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein napC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity. HAMAP MF_01630
Catalytic activity	Nitrite + acceptor = nitrate + reduced acceptor. HAMAP MF_01630
Cofactor	Binds 1 4Fe-4S cluster By similarity. HAMAP MF_01630 Binds 1 molybdenum ion per subunit By similarity. HAMAP MF_01630 Binds 2 molybdopterin guanine dinucleotide (MGD) groups per subunit By similarity. HAMAP MF_01630
Subunit structure	Interacts with napB By similarity. HAMAP MF_01630
Subcellular location	Periplasm By similarity HAMAP MF_01630.
Post-translational modification	Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. HAMAP MF_01630
Sequence similarities	Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/napA/narB subfamily.

Ontologies

Keywords
Biological process	Electron transport Nitrate assimilation Transport
Cellular component	Periplasm
Domain	Signal
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding Molybdenum
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	Mo-molybdopterin cofactor biosynthetic process Inferred from electronic annotation. Source: HAMAP electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW nitrate assimilation Inferred from electronic annotation. Source: HAMAP transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: HAMAP molybdenum ion binding Inferred from electronic annotation. Source: UniProtKB-KW nitrate reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 29	29	Tat-type signal Potential
	Chain	30 – 832	803	Periplasmic nitrate reductase HAMAP MF_01630		PRO_0000045982
Sites
	Metal binding	48	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	51	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	55	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	83	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q487G4-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 67EC37296448C080	FASTA	832	93,469
	10 20 30 40 50 60 MTINRREFIK ANAIAAAAAV AGVSVPAVAS NLITSSDITK LKWDKAACRF CGTGCSVNVG 70 80 90 100 110 120 VMDGKVVATH GDIKSPVNKG LSCVKGYFLS KIMYGKDRLT TPLLRMTNGK YDKEGEFTPI 130 140 150 160 170 180 SWDQAFNVMA EKANDALKKD GPEALGMFGS GQWTVQEGYA AVKLMKAGFR TNNIDPNARH 190 200 210 220 230 240 CMASAVGGFM RTFGIDEPMG CYDDLEAADA FVLWGSNMAE MHPILWTRLT DRRLSAPHVK 250 260 270 280 290 300 VAVLSTFEHR SFDLADNGMI FTPQTDLAIL NYIANYIIQT GRVNKDFVSK HTNFRLGETD 310 320 330 340 350 360 IGYGLRPEHP LEQKAKNNGK TKGSSTPIDF DEYAKFVSTY TVESVSKLSG VPEHKLKELA 370 380 390 400 410 420 EMYADPKIKV TSFWTMGFNQ HTRGVWANNL VYNIHLLTGK ISTPGNSPFS LTGQPSACGT 430 440 450 460 470 480 AREVGTFSHR LPADMVVKNP KHRAIAEKIW DLPEGTIPAK PGYHAVLQNR MLKDGKLNFY 490 500 510 520 530 540 WVQCNNNMQA AANINEEAYP GYRNPKNFIV VSDPYPTVTA QAADLILPTA MWVEKEGMYG 550 560 570 580 590 600 NAERRTQSWY QMVEAPEGAK SDMWQLVEFS KRFKVEDVWP EELIAKKPEV RGKTLFDVLY 610 620 630 640 650 660 KDASVGKFPL SEIPEDRLND ESRDFGFYIQ KGLFEEYASF GRGHAHDLAP YDRYHKERGL 670 680 690 700 710 720 RWPVVNGKET KWRFKEGSDP YCKPGSDWDF YGKPDGRAVI FALPYEPAAE SPDEEYDLWL 730 740 750 760 770 780 STGRVLEHWH SGSMTQRVPE LYKAMPDALV YMHPDDAKKR NMRRGDLVKL ISRRGEVQTR 790 800 810 820 830 VETRGRNKPP VGLVYMPWFD ASRLVNKVTL DATDPLSKET DYKKCAIKIV KV

References

[1]

"The psychrophilic lifestyle as revealed by the genome sequence of Colwellia psychrerythraea 34H through genomic and proteomic analyses." Methe B.A., Nelson K.E., Deming J.W., Momen B., Melamud E., Zhang X., Moult J., Madupu R., Nelson W.C., Dodson R.J., Brinkac L.M., Daugherty S.C., Durkin A.S., DeBoy R.T., Kolonay J.F., Sullivan S.A., Zhou L., Davidsen T.M. , Wu M., Huston A.L., Lewis M., Weaver B., Weidman J.F., Khouri H., Utterback T.R., Feldblyum T.V., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 102:10913-10918(2005) [PubMed: 16043709] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000083 Genomic DNA. Translation: AAZ26086.1.
RefSeq	YP_267801.1.
3D structure databases
SMR	Q487G4. Positions 41-831.
ModBase	Search...
Protein-protein interaction databases
STRING	Q487G4.
Genome annotation databases
GeneID	3520335.
GenomeReviews	Gene locus CPS_1057 in contig CP000083_GR.
KEGG	cps:CPS_1057.
NMPDR	fig|167879.3.peg.1019.
TIGR	CPS_1057.
Phylogenomic databases
eggNOG	COG0243.
HOGENOM	HBG715033.
OMA	NAINREF.
Enzyme and pathway databases
BioCyc	CPSY167879:CPS_1057-MONOMER.
Family and domain databases
HAMAP	MF_01630. Nitrate_reduct. [Tree]
InterPro	IPR009010. Asp_de-COase-like_fold. IPR006657. MoPterin_dinucl-bd_dom. IPR006656. Mopterin_OxRdtase. IPR006963. Mopterin_OxRdtase_Fe4S4_dom. IPR006655. Mopterin_OxRdtase_prok_CS. IPR010051. NO3_reductase_lsu_periplasm. IPR006311. TAT_signal. IPR017909. Twin_arg_translocation_Tat. [Graphical view]
Gene3D	G3DSA:2.40.40.20. Asp_decarboxylase-like_fold. 1 hit.
Pfam	PF04879. Molybdop_Fe4S4. 1 hit. PF00384. Molybdopterin. 1 hit. PF01568. Molydop_binding. 1 hit. [Graphical view]
TIGRFAMs	TIGR01706. NAPA. 1 hit. TIGR01409. TAT_signal_seq. 1 hit.
PROSITE	PS00551. MOLYBDOPTERIN_PROK_1. 1 hit. PS00490. MOLYBDOPTERIN_PROK_2. False negative. PS00932. MOLYBDOPTERIN_PROK_3. False negative. PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NAPA_COLP3

Accession

Primary (citable) accession number: Q487G4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 7, 2006
Last sequence update:	September 13, 2005
Last modified:	February 9, 2010
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)