Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q487G4

- NAPA_COLP3

UniProt

Q487G4 - NAPA_COLP3

Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Colwellia psychrerythraea (strain 34H / ATCC BAA-681) (Vibrio psychroerythus)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 66 (01 Oct 2014)
      Sequence version 1 (13 Sep 2005)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism.UniRule annotation

    Catalytic activityi

    Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster.UniRule annotation
    Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi48 – 481Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi51 – 511Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi55 – 551Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi83 – 831Iron-sulfur (4Fe-4S)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. electron carrier activity Source: UniProtKB-HAMAP
    3. iron ion binding Source: UniProtKB-HAMAP
    4. molybdenum ion binding Source: InterPro
    5. nitrate reductase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
    2. nitrate assimilation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Nitrate assimilation, Transport

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

    Enzyme and pathway databases

    BioCyciCPSY167879:GI48-1057-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Periplasmic nitrate reductaseUniRule annotation (EC:1.7.99.4UniRule annotation)
    Gene namesi
    Name:napAUniRule annotation
    Ordered Locus Names:CPS_1057
    OrganismiColwellia psychrerythraea (strain 34H / ATCC BAA-681) (Vibrio psychroerythus)
    Taxonomic identifieri167879 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesColwelliaceaeColwellia
    ProteomesiUP000000547: Chromosome

    Subcellular locationi

    Periplasm UniRule annotation

    GO - Cellular componenti

    1. periplasmic space Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Periplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2929Tat-type signalUniRule annotationAdd
    BLAST
    Chaini30 – 832803Periplasmic nitrate reductasePRO_0000045982Add
    BLAST

    Post-translational modificationi

    Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

    Interactioni

    Subunit structurei

    Interacts with NapB.UniRule annotation

    Protein-protein interaction databases

    STRINGi167879.CPS_1057.

    Structurei

    3D structure databases

    ProteinModelPortaliQ487G4.
    SMRiQ487G4. Positions 41-831.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini41 – 97574Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation
    Contains 1 4Fe-4S Mo/W bis-MGD-type domain.UniRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG0243.
    HOGENOMiHOG000031441.
    KOiK02567.
    OMAiQMVYNLH.
    OrthoDBiEOG6CVV7G.

    Family and domain databases

    HAMAPiMF_01630. Nitrate_reduct.
    InterProiIPR009010. Asp_de-COase-like_dom.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR027467. MopterinOxRdtase_cofactor_BS.
    IPR010051. Periplasm_NO3_reductase_lsu.
    IPR006311. TAT_signal.
    IPR019546. TAT_signal_bac_arc.
    [Graphical view]
    PfamiPF04879. Molybdop_Fe4S4. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF01568. Molydop_binding. 1 hit.
    [Graphical view]
    SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
    [Graphical view]
    SUPFAMiSSF50692. SSF50692. 1 hit.
    TIGRFAMsiTIGR01706. NAPA. 1 hit.
    TIGR01409. TAT_signal_seq. 1 hit.
    PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
    PS51318. TAT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q487G4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTINRREFIK ANAIAAAAAV AGVSVPAVAS NLITSSDITK LKWDKAACRF    50
    CGTGCSVNVG VMDGKVVATH GDIKSPVNKG LSCVKGYFLS KIMYGKDRLT 100
    TPLLRMTNGK YDKEGEFTPI SWDQAFNVMA EKANDALKKD GPEALGMFGS 150
    GQWTVQEGYA AVKLMKAGFR TNNIDPNARH CMASAVGGFM RTFGIDEPMG 200
    CYDDLEAADA FVLWGSNMAE MHPILWTRLT DRRLSAPHVK VAVLSTFEHR 250
    SFDLADNGMI FTPQTDLAIL NYIANYIIQT GRVNKDFVSK HTNFRLGETD 300
    IGYGLRPEHP LEQKAKNNGK TKGSSTPIDF DEYAKFVSTY TVESVSKLSG 350
    VPEHKLKELA EMYADPKIKV TSFWTMGFNQ HTRGVWANNL VYNIHLLTGK 400
    ISTPGNSPFS LTGQPSACGT AREVGTFSHR LPADMVVKNP KHRAIAEKIW 450
    DLPEGTIPAK PGYHAVLQNR MLKDGKLNFY WVQCNNNMQA AANINEEAYP 500
    GYRNPKNFIV VSDPYPTVTA QAADLILPTA MWVEKEGMYG NAERRTQSWY 550
    QMVEAPEGAK SDMWQLVEFS KRFKVEDVWP EELIAKKPEV RGKTLFDVLY 600
    KDASVGKFPL SEIPEDRLND ESRDFGFYIQ KGLFEEYASF GRGHAHDLAP 650
    YDRYHKERGL RWPVVNGKET KWRFKEGSDP YCKPGSDWDF YGKPDGRAVI 700
    FALPYEPAAE SPDEEYDLWL STGRVLEHWH SGSMTQRVPE LYKAMPDALV 750
    YMHPDDAKKR NMRRGDLVKL ISRRGEVQTR VETRGRNKPP VGLVYMPWFD 800
    ASRLVNKVTL DATDPLSKET DYKKCAIKIV KV 832
    Length:832
    Mass (Da):93,469
    Last modified:September 13, 2005 - v1
    Checksum:i67EC37296448C080
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000083 Genomic DNA. Translation: AAZ26086.1.
    RefSeqiWP_011041895.1. NC_003910.7.
    YP_267801.1. NC_003910.7.

    Genome annotation databases

    EnsemblBacteriaiAAZ26086; AAZ26086; CPS_1057.
    GeneIDi3520335.
    KEGGicps:CPS_1057.
    PATRICi21465389. VBIColPsy94388_0947.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000083 Genomic DNA. Translation: AAZ26086.1 .
    RefSeqi WP_011041895.1. NC_003910.7.
    YP_267801.1. NC_003910.7.

    3D structure databases

    ProteinModelPortali Q487G4.
    SMRi Q487G4. Positions 41-831.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 167879.CPS_1057.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAZ26086 ; AAZ26086 ; CPS_1057 .
    GeneIDi 3520335.
    KEGGi cps:CPS_1057.
    PATRICi 21465389. VBIColPsy94388_0947.

    Phylogenomic databases

    eggNOGi COG0243.
    HOGENOMi HOG000031441.
    KOi K02567.
    OMAi QMVYNLH.
    OrthoDBi EOG6CVV7G.

    Enzyme and pathway databases

    BioCyci CPSY167879:GI48-1057-MONOMER.

    Family and domain databases

    HAMAPi MF_01630. Nitrate_reduct.
    InterProi IPR009010. Asp_de-COase-like_dom.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR027467. MopterinOxRdtase_cofactor_BS.
    IPR010051. Periplasm_NO3_reductase_lsu.
    IPR006311. TAT_signal.
    IPR019546. TAT_signal_bac_arc.
    [Graphical view ]
    Pfami PF04879. Molybdop_Fe4S4. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF01568. Molydop_binding. 1 hit.
    [Graphical view ]
    SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50692. SSF50692. 1 hit.
    TIGRFAMsi TIGR01706. NAPA. 1 hit.
    TIGR01409. TAT_signal_seq. 1 hit.
    PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
    PS51318. TAT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 34H / ATCC BAA-681.

    Entry informationi

    Entry nameiNAPA_COLP3
    AccessioniPrimary (citable) accession number: Q487G4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 7, 2006
    Last sequence update: September 13, 2005
    Last modified: October 1, 2014
    This is version 66 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3