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Q487G4 (NAPA_COLP3) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Periplasmic nitrate reductase

EC=1.7.99.4
Gene names
Name:napA
Ordered Locus Names:CPS_1057
OrganismColwellia psychrerythraea (strain 34H / ATCC BAA-681) (Vibrio psychroerythus) [Complete proteome] [HAMAP]
Taxonomic identifier167879 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesColwelliaceaeColwellia

Protein attributes

Sequence length832 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity. HAMAP-Rule MF_01630

Catalytic activity

Nitrite + acceptor = nitrate + reduced acceptor. HAMAP-Rule MF_01630

Cofactor

Binds 1 4Fe-4S cluster By similarity. HAMAP-Rule MF_01630

Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Subunit structure

Interacts with NapB By similarity. HAMAP-Rule MF_01630

Subcellular location

Periplasm By similarity HAMAP-Rule MF_01630.

Post-translational modification

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. HAMAP-Rule MF_01630

Sequence similarities

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.

Contains 1 4Fe-4S Mo/W bis-MGD-type domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2929Tat-type signal Potential
Chain30 – 832803Periplasmic nitrate reductase HAMAP-Rule MF_01630
PRO_0000045982

Regions

Domain41 – 97574Fe-4S Mo/W bis-MGD-type

Sites

Metal binding481Iron-sulfur (4Fe-4S) By similarity
Metal binding511Iron-sulfur (4Fe-4S) By similarity
Metal binding551Iron-sulfur (4Fe-4S) By similarity
Metal binding831Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q487G4 [UniParc].

Last modified September 13, 2005. Version 1.
Checksum: 67EC37296448C080

FASTA83293,469
        10         20         30         40         50         60 
MTINRREFIK ANAIAAAAAV AGVSVPAVAS NLITSSDITK LKWDKAACRF CGTGCSVNVG 

        70         80         90        100        110        120 
VMDGKVVATH GDIKSPVNKG LSCVKGYFLS KIMYGKDRLT TPLLRMTNGK YDKEGEFTPI 

       130        140        150        160        170        180 
SWDQAFNVMA EKANDALKKD GPEALGMFGS GQWTVQEGYA AVKLMKAGFR TNNIDPNARH 

       190        200        210        220        230        240 
CMASAVGGFM RTFGIDEPMG CYDDLEAADA FVLWGSNMAE MHPILWTRLT DRRLSAPHVK 

       250        260        270        280        290        300 
VAVLSTFEHR SFDLADNGMI FTPQTDLAIL NYIANYIIQT GRVNKDFVSK HTNFRLGETD 

       310        320        330        340        350        360 
IGYGLRPEHP LEQKAKNNGK TKGSSTPIDF DEYAKFVSTY TVESVSKLSG VPEHKLKELA 

       370        380        390        400        410        420 
EMYADPKIKV TSFWTMGFNQ HTRGVWANNL VYNIHLLTGK ISTPGNSPFS LTGQPSACGT 

       430        440        450        460        470        480 
AREVGTFSHR LPADMVVKNP KHRAIAEKIW DLPEGTIPAK PGYHAVLQNR MLKDGKLNFY 

       490        500        510        520        530        540 
WVQCNNNMQA AANINEEAYP GYRNPKNFIV VSDPYPTVTA QAADLILPTA MWVEKEGMYG 

       550        560        570        580        590        600 
NAERRTQSWY QMVEAPEGAK SDMWQLVEFS KRFKVEDVWP EELIAKKPEV RGKTLFDVLY 

       610        620        630        640        650        660 
KDASVGKFPL SEIPEDRLND ESRDFGFYIQ KGLFEEYASF GRGHAHDLAP YDRYHKERGL 

       670        680        690        700        710        720 
RWPVVNGKET KWRFKEGSDP YCKPGSDWDF YGKPDGRAVI FALPYEPAAE SPDEEYDLWL 

       730        740        750        760        770        780 
STGRVLEHWH SGSMTQRVPE LYKAMPDALV YMHPDDAKKR NMRRGDLVKL ISRRGEVQTR 

       790        800        810        820        830 
VETRGRNKPP VGLVYMPWFD ASRLVNKVTL DATDPLSKET DYKKCAIKIV KV 

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References

[1]"The psychrophilic lifestyle as revealed by the genome sequence of Colwellia psychrerythraea 34H through genomic and proteomic analyses."
Methe B.A., Nelson K.E., Deming J.W., Momen B., Melamud E., Zhang X., Moult J., Madupu R., Nelson W.C., Dodson R.J., Brinkac L.M., Daugherty S.C., Durkin A.S., DeBoy R.T., Kolonay J.F., Sullivan S.A., Zhou L., Davidsen T.M. expand/collapse author list , Wu M., Huston A.L., Lewis M., Weaver B., Weidman J.F., Khouri H., Utterback T.R., Feldblyum T.V., Fraser C.M.
Proc. Natl. Acad. Sci. U.S.A. 102:10913-10918(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 34H / ATCC BAA-681.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000083 Genomic DNA. Translation: AAZ26086.1.
RefSeqYP_267801.1. NC_003910.7.

3D structure databases

ProteinModelPortalQ487G4.
SMRQ487G4. Positions 41-831.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING167879.CPS_1057.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAZ26086; AAZ26086; CPS_1057.
GeneID3520335.
KEGGcps:CPS_1057.
PATRIC21465389. VBIColPsy94388_0947.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0243.
HOGENOMHOG000031441.
KOK02567.
OMADAFGFYI.
OrthoDBEOG6CVV7G.
ProtClustDBPRK13532.

Enzyme and pathway databases

BioCycCPSY167879:GI48-1057-MONOMER.

Family and domain databases

HAMAPMF_01630. Nitrate_reduct.
InterProIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMSSF50692. SSF50692. 1 hit.
TIGRFAMsTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNAPA_COLP3
AccessionPrimary (citable) accession number: Q487G4
Entry history
Integrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: September 13, 2005
Last modified: February 19, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families