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Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Colwellia psychrerythraea (strain 34H / ATCC BAA-681) (Vibrio psychroerythus)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism.UniRule annotation

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation
  • Mo-bis(molybdopterin guanine dinucleotide)UniRule annotationNote: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi48 – 481Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi51 – 511Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi55 – 551Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi83 – 831Iron-sulfur (4Fe-4S)UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Nitrate assimilation, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BioCyciCPSY167879:GI48-1057-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductaseUniRule annotation (EC:1.7.99.4UniRule annotation)
Gene namesi
Name:napAUniRule annotation
Ordered Locus Names:CPS_1057
OrganismiColwellia psychrerythraea (strain 34H / ATCC BAA-681) (Vibrio psychroerythus)
Taxonomic identifieri167879 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesColwelliaceaeColwellia
ProteomesiUP000000547 Componenti: Chromosome

Subcellular locationi

  • Periplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2929Tat-type signalUniRule annotationAdd
BLAST
Chaini30 – 832803Periplasmic nitrate reductasePRO_0000045982Add
BLAST

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

Interactioni

Subunit structurei

Interacts with NapB.UniRule annotation

Protein-protein interaction databases

STRINGi167879.CPS_1057.

Structurei

3D structure databases

ProteinModelPortaliQ487G4.
SMRiQ487G4. Positions 41-831.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini41 – 97574Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation
Contains 1 4Fe-4S Mo/W bis-MGD-type domain.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0243.
HOGENOMiHOG000031441.
KOiK02567.
OMAiMARRDFI.
OrthoDBiEOG6CVV7G.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct.
InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q487G4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTINRREFIK ANAIAAAAAV AGVSVPAVAS NLITSSDITK LKWDKAACRF
60 70 80 90 100
CGTGCSVNVG VMDGKVVATH GDIKSPVNKG LSCVKGYFLS KIMYGKDRLT
110 120 130 140 150
TPLLRMTNGK YDKEGEFTPI SWDQAFNVMA EKANDALKKD GPEALGMFGS
160 170 180 190 200
GQWTVQEGYA AVKLMKAGFR TNNIDPNARH CMASAVGGFM RTFGIDEPMG
210 220 230 240 250
CYDDLEAADA FVLWGSNMAE MHPILWTRLT DRRLSAPHVK VAVLSTFEHR
260 270 280 290 300
SFDLADNGMI FTPQTDLAIL NYIANYIIQT GRVNKDFVSK HTNFRLGETD
310 320 330 340 350
IGYGLRPEHP LEQKAKNNGK TKGSSTPIDF DEYAKFVSTY TVESVSKLSG
360 370 380 390 400
VPEHKLKELA EMYADPKIKV TSFWTMGFNQ HTRGVWANNL VYNIHLLTGK
410 420 430 440 450
ISTPGNSPFS LTGQPSACGT AREVGTFSHR LPADMVVKNP KHRAIAEKIW
460 470 480 490 500
DLPEGTIPAK PGYHAVLQNR MLKDGKLNFY WVQCNNNMQA AANINEEAYP
510 520 530 540 550
GYRNPKNFIV VSDPYPTVTA QAADLILPTA MWVEKEGMYG NAERRTQSWY
560 570 580 590 600
QMVEAPEGAK SDMWQLVEFS KRFKVEDVWP EELIAKKPEV RGKTLFDVLY
610 620 630 640 650
KDASVGKFPL SEIPEDRLND ESRDFGFYIQ KGLFEEYASF GRGHAHDLAP
660 670 680 690 700
YDRYHKERGL RWPVVNGKET KWRFKEGSDP YCKPGSDWDF YGKPDGRAVI
710 720 730 740 750
FALPYEPAAE SPDEEYDLWL STGRVLEHWH SGSMTQRVPE LYKAMPDALV
760 770 780 790 800
YMHPDDAKKR NMRRGDLVKL ISRRGEVQTR VETRGRNKPP VGLVYMPWFD
810 820 830
ASRLVNKVTL DATDPLSKET DYKKCAIKIV KV
Length:832
Mass (Da):93,469
Last modified:September 13, 2005 - v1
Checksum:i67EC37296448C080
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000083 Genomic DNA. Translation: AAZ26086.1.
RefSeqiWP_011041895.1. NC_003910.7.

Genome annotation databases

EnsemblBacteriaiAAZ26086; AAZ26086; CPS_1057.
KEGGicps:CPS_1057.
PATRICi21465389. VBIColPsy94388_0947.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000083 Genomic DNA. Translation: AAZ26086.1.
RefSeqiWP_011041895.1. NC_003910.7.

3D structure databases

ProteinModelPortaliQ487G4.
SMRiQ487G4. Positions 41-831.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi167879.CPS_1057.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAZ26086; AAZ26086; CPS_1057.
KEGGicps:CPS_1057.
PATRICi21465389. VBIColPsy94388_0947.

Phylogenomic databases

eggNOGiCOG0243.
HOGENOMiHOG000031441.
KOiK02567.
OMAiMARRDFI.
OrthoDBiEOG6CVV7G.

Enzyme and pathway databases

BioCyciCPSY167879:GI48-1057-MONOMER.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct.
InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 34H / ATCC BAA-681.

Entry informationi

Entry nameiNAPA_COLP3
AccessioniPrimary (citable) accession number: Q487G4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: September 13, 2005
Last modified: July 22, 2015
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.