Reviewed, UniProtKB/Swiss-Prot Q487A4 (ACEK_COLP3)

Names and origin

Protein names

Recommended name:
    Isocitrate dehydrogenase kinase/phosphatase
      Short name=IDH kinase/phosphatase
      Short name=IDHK/P
    EC=2.7.11.5
    EC=3.1.3.-

Gene names

Name:	aceK
Ordered Locus Names:	CPS_1118

Organism

Colwellia psychrerythraea (strain 34H / ATCC BAA-681) (Vibrio psychroerythus) [Complete proteome] [HAMAP]

Taxonomic identifier

167879 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Colwelliaceae › Colwellia

Protein attributes

Sequence length	589 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Bifunctional enzyme which can phosphorylate or dephosphorylate isocitrate dehydrogenase (IDH) on a specific serine residue. This is a regulatory mechanism which enables bacteria to bypass the Krebs cycle via the glyoxylate shunt in response to the source of carbon. When bacteria are grown on glucose, IDH is fully active and unphosphorylated, but when grown on acetate or ethanol, the activity of IDH declines drastically concomitant with its phosphorylation By similarity. HAMAP MF_00747
Catalytic activity	ATP + [isocitrate dehydrogenase (NADP+)] = ADP + [isocitrate dehydrogenase (NADP+)] phosphate. HAMAP MF_00747
Subcellular location	Cytoplasm By similarity HAMAP MF_00747.
Sequence similarities	Belongs to the aceK family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Ligand	ATP-binding NADP Nucleotide-binding
Molecular function	Hydrolase Kinase Protein phosphatase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glucose metabolic process Inferred from electronic annotation. Source: InterPro glyoxylate cycle Inferred from electronic annotation. Source: HAMAP tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP [isocitrate dehydrogenase (NADP+)] kinase activity Inferred from electronic annotation. Source: HAMAP phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 589	589	Isocitrate dehydrogenase kinase/phosphatase HAMAP MF_00747		PRO_0000288285
Regions
	Nucleotide binding	317 – 323	7	ATP By similarity
Sites
	Active site	373	1	By similarity
	Binding site	338	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q487A4-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 71EF3D2F0FA2D5BE	FASTA	589	68,578
	10 20 30 40 50 60 MKDELALTIA NTIINGFERH FAIFTEITQS ARNRFQQCQW NEIHRSARAR TNFYDERVKE 70 80 90 100 110 120 TFNDIKEDFN ISSLDDALWQ RVKAVYSDLL INHKQPELAE TFYNSVFCHL FERKYYHNDY 130 140 150 160 170 180 IYVESTAHRL DDKTQPEIYT SYQPKELGLK QTICDIMNSH RTVIPFEDLD RDVDALINTF 190 200 210 220 230 240 RRKAHKTRVK LEDLKFDILN FTFYRNKGAY LIGRVLSPAG ETPFIIAVLN NEKGGLYIDA 250 260 270 280 290 300 LLTSSESMAV VFGFARAYFF VDCEHPYALV NFLQGLMPHK TKADLYSAIG FHKQGKTQFY 310 320 330 340 350 360 RDFLNHLDSS DDQFELAAGI KGMVMSVFTL PSYPYVFKII KDKFSPSKNI TKKDVKGKYR 370 380 390 400 410 420 LVKLHDRVGR MADTMEYSEV AFPKSRFNDE LLAELQKVAP SIIRYEGEGE EALIIIEHLY 430 440 450 460 470 480 IERRMVPLNL YLMDALKNKA QQKIDDALFG YGQAIKQLIS ADIFPGDMLL KNFGVTRHGR 490 500 510 520 530 540 VIFYDYDEIA YMNEINFRVK PKAVTEEQLY AAEPWYSVMP GDMFPEELAT FALANPSYLK 550 560 570 580 AFKIHHEDLL TAAYWQQCQQ DVANGIYKDV FPYPDKYRFC NLSFGSIKR

References

[1]

"The psychrophilic lifestyle as revealed by the genome sequence of Colwellia psychrerythraea 34H through genomic and proteomic analyses." Methe B.A., Nelson K.E., Deming J.W., Momen B., Melamud E., Zhang X., Moult J., Madupu R., Nelson W.C., Dodson R.J., Brinkac L.M., Daugherty S.C., Durkin A.S., DeBoy R.T., Kolonay J.F., Sullivan S.A., Zhou L., Davidsen T.M. , Wu M., Huston A.L., Lewis M., Weaver B., Weidman J.F., Khouri H., Utterback T.R., Feldblyum T.V., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 102:10913-10918(2005) [PubMed: 16043709] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000083 Genomic DNA. Translation: AAZ27754.1.
RefSeq	YP_267861.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q487A4.
Genome annotation databases
GeneID	3522013.
GenomeReviews	Gene locus CPS_1118 in contig CP000083_GR.
KEGG	cps:CPS_1118.
NMPDR	fig|167879.3.peg.1079.
TIGR	CPS_1118.
Phylogenomic databases
eggNOG	COG4579.
HOGENOM	HBG298593.
OMA	RMTPLNI.
Enzyme and pathway databases
BioCyc	CPSY167879:CPS_1118-MONOMER.
Family and domain databases
HAMAP	MF_00747. AceK. [Tree]
InterPro	IPR010452. Isocitrate_DH_AceK. [Graphical view]
Pfam	PF06315. AceK. 1 hit. [Graphical view]
PIRSF	PIRSF000719. AceK. 1 hit.
ProDom	PD043552. Isocitrate_DH_AceK. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

ACEK_COLP3

Accession

Primary (citable) accession number: Q487A4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	September 13, 2005
Last modified:	January 19, 2010
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)