Reviewed, UniProtKB/Swiss-Prot Q48768 (CHEA_LISMO)

Names and origin

Protein names

Recommended name:
    Chemotaxis protein cheA
    EC=2.7.13.3

Gene names

Name:	cheA
Ordered Locus Names:	lmo0692

Organism

Listeria monocytogenes [Complete proteome] [HAMAP]

Taxonomic identifier

1639 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Listeriaceae › Listeria

Protein attributes

Sequence length	618 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in the transmission of sensory signals from the chemoreceptors to the flagellar motors. CheA is autophosphorylated; it can transfer its phosphate group to either cheB or cheY By similarity.
Catalytic activity	ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Contains 1 cheW-like domain. Contains 1 histidine kinase domain. Contains 1 HPt domain.

Ontologies

Keywords
Biological process	Chemotaxis Two-component regulatory system
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell motion Inferred from electronic annotation. Source: InterPro chemotaxis Inferred from electronic annotation. Source: UniProtKB-KW peptidyl-histidine phosphorylation Inferred from electronic annotation. Source: InterPro two-component signal transduction system (phosphorelay) Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW two-component sensor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 618	618	Chemotaxis protein cheA		PRO_0000074715
Regions
	Domain	1 – 102	102	HPt
	Domain	233 – 488	256	Histidine kinase
	Domain	490 – 618	129	CheW-like
Amino acid modifications
	Modified residue	45	1	Phosphohistidine; by autocatalysis By similarity
Experimental info
	Sequence conflict	132	1	T → A in CAA53765. Ref.1
	Sequence conflict	140	1	A → Q in CAA53765. Ref.1
	Sequence conflict	197	1	D → E in CAA53765. Ref.1
	Sequence conflict	200	1	K → E in CAA53765. Ref.1
	Sequence conflict	203	1	I → V in CAA53765. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q48768-1 [UniParc]. Last modified December 5, 2001. Version 2. Checksum: 63B560C05D8E941C	FASTA	618	68,628
	10 20 30 40 50 60 MTTNMLDLFI EEASEHLQAL NDNLLQLEKD PTNGQLVSEI FRSAHTFKGM SATMGFQQVA 70 80 90 100 110 120 DLTHAMENVL DEVRNNRLAV TEHLVDIIFT CTSHLETMVS DIQHGGQGAA DISKTVADLE 130 140 150 160 170 180 ALLHPEQETD LTVEKTYRIA IQIEEAAILK AVRAVMCLER LAEMGIISET TPDREAIELE 190 200 210 220 230 240 EFEQSFEVVL ESAQTKDEIK AVILDISEIE KVTVTEEVEE VQVIEPIKKA AKQTTKRLEN 250 260 270 280 290 300 KTIRVQLEKI EKLMNVFEES VIERARIDEI AEKTNNKELM EHLGRFSSIS KEIQNGLLNM 310 320 330 340 350 360 RMVPVDSVFN RFPKMVRTLA KELGKKIDLV IEGADTEVDK IVIDEIGDPL VHLIRNSVDH 370 380 390 400 410 420 GAETVEVRRK NGKNETATIN LKAFHSGNNV VIEIADDGAG INKRKVLEKA IAKNVVTRAE 430 440 450 460 470 480 STKMTDAEIF DLLFDSGFST ADQVSDLSGR GVGLDVVRNT ILKIGGKISV ESSENAGSTF 490 500 510 520 530 540 RIEIPLTLSI IQSMLVATSE RRYAVPLANV AEAITINPAD IQHVHGKDLI NYRETIIEVL 550 560 570 580 590 600 DLGECFHETP LNDTDELLLL VVKNAKRTFG LIIKDIIGQR EIVLKTLGGF FSESQIAFSG 610 ATILGDGRVV LILNLETF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of two putative Listeria monocytogenes genes encoding polypeptides homologous to the sensor protein CheA and the response regulator CheY of chemotaxis." Dons L., Olsen J.E., Rasmussen O.F. DNA Seq. 4:301-311(1994) [PubMed: 7803815] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 12067.
[2]	"Comparative genomics of Listeria species." Glaser P., Frangeul L., Buchrieser C., Rusniok C., Amend A., Baquero F., Berche P., Bloecker H., Brandt P., Chakraborty T., Charbit A., Chetouani F., Couve E., de Daruvar A., Dehoux P., Domann E., Dominguez-Bernal G., Duchaud E. , Durant L., Dussurget O., Entian K.-D., Fsihi H., Garcia-del Portillo F., Garrido P., Gautier L., Goebel W., Gomez-Lopez N., Hain T., Hauf J., Jackson D., Jones L.-M., Kaerst U., Kreft J., Kuhn M., Kunst F., Kurapkat G., Madueno E., Maitournam A., Mata Vicente J., Ng E., Nedjari H., Nordsiek G., Novella S., de Pablos B., Perez-Diaz J.-C., Purcell R., Remmel B., Rose M., Schlueter T., Simoes N., Tierrez A., Vazquez-Boland J.-A., Voss H., Wehland J., Cossart P. Science 294:849-852(2001) [PubMed: 11679669] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-679 / EGD-e / Serovar 1/2a.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X76170 Genomic DNA. Translation: CAA53765.1. AL591976 Genomic DNA. Translation: CAC98770.1.
PIR	AD1161.
RefSeq	NP_464219.1.
3D structure databases
HSSP	HSSP built from PDB template 1I58 based on UniProtKB Q56310.
ModBase	Search...
Genome annotation databases
GeneID	985032.
GenomeReviews	Gene locus lmo0692 in contig AL591824_GR.
KEGG	lmo:lmo0692.
Organism-specific databases
ListiList	LMO00692.
CMR	Search...
Phylogenomic databases
HOGENOM	Q48768.
OMA	ATSERRY.
Enzyme and pathway databases
BioCyc	LMON169963:LMO0692-MON.
BRENDA	2.7.13.3. 96770.
Family and domain databases
InterPro	IPR003594. ATP_bd_ATPase. IPR002545. CheW. IPR004358. Sig_transdc_His_kin-like_C. IPR008207. Sig_transdc_His_kin_P-trf. IPR004105. Sig_transdc_His_kin_subgr_dim. IPR005467. Sig_transdc_His_kinase_core. IPR010808. Sig_transdc_His_kinase_P2-bd. [Graphical view]
Gene3D	G3DSA:3.30.565.10. ATP_bd_ATPase. 1 hit.
Pfam	PF01584. CheW. 1 hit. PF02895. H-kinase_dim. 1 hit. PF02518. HATPase_c. 1 hit. PF01627. Hpt. 1 hit. PF07194. P2. 1 hit. [Graphical view]
PRINTS	PR00344. BCTRLSENSOR.
ProDom	PD003142. Hpt_N. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00260. CheW. 1 hit. SM00387. HATPase_c. 1 hit. SM00073. HPT. 1 hit. [Graphical view]
PROSITE	PS50851. CHEW. 1 hit. PS50109. HIS_KIN. 1 hit. PS50894. HPT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHEA_LISMO

Accession

Primary (citable) accession number: Q48768

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	December 5, 2001
Last modified:	November 3, 2009
This is version 70 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)