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Q48745 (DDL_LEUME) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
OrganismLeuconostoc mesenteroides
Taxonomic identifier1245 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesLeuconostoc

Protein attributes

Sequence length377 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 377377D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_0000177836

Regions

Domain140 – 349210ATP-grasp
Nucleotide binding170 – 22556ATP By similarity

Sites

Metal binding3031Magnesium or manganese 1 By similarity
Metal binding3161Magnesium or manganese 1 By similarity
Metal binding3161Magnesium or manganese 2 By similarity
Metal binding3181Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q48745 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: A4EA56586FE0B65E

FASTA37741,914
        10         20         30         40         50         60 
MTKKRVALIF GGNSSEHDVS KRSAQNFYNA IEATDKYEII VFAIAQNGFF LDTESSKKIL 

        70         80         90        100        110        120 
ALEDEQPIVD AFMKTVDTSD PLARIHALKS AGDFDIFFPV VHGNLGEDGT LQGLFKLLDK 

       130        140        150        160        170        180 
PYVGAPLRGH AVSFDKALTK ELLTVNGIRN TKYIVVDPES ANNWSWDKIV AELGNIVFVK 

       190        200        210        220        230        240 
AANQGSSVGI SRVTNAEEYT EALSDSFQYD YKVLIEEAVN GARELEVGVI GNDQPLVSEI 

       250        260        270        280        290        300 
GAHTVPNQGS GDGWYDYNNK FVDNSAVHFE IPAQLSPEVT KEVKQMALDA YKVLNLRGEA 

       310        320        330        340        350        360 
RMDFLLDENN VPYLGEPNTL PGFTNMSLFK RLWDYSDINN AKLVDMLIDY GFEDFAQNKK 

       370 
LSYSFVSLGE EKIGKFN 

« Hide

References

[1]"Analysis of genes encoding D-alanine:D-alanine ligase-related enzymes in Leuconostoc mesenteroides and Lactobacillus spp."
Elisha B.G., Courvalin P.
Gene 152:79-83(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: BM4298.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U08909 Genomic DNA. Translation: AAA87679.1.

3D structure databases

ProteinModelPortalQ48745.
SMRQ48745. Positions 3-362.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_LEUME
AccessionPrimary (citable) accession number: Q48745
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 1, 1996
Last modified: February 19, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways