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Q48743 (AMPC_LYSLA) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-lactamase

EC=3.5.2.6
Alternative name(s):
Cephalosporinase
OrganismLysobacter lactamgenus
Taxonomic identifier39596 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaXanthomonadalesXanthomonadaceaeLysobacter

Protein attributes

Sequence length385 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

This protein is a serine beta-lactamase with a substrate specificity for cephalosporins.

Catalytic activity

A beta-lactam + H2O = a substituted beta-amino acid.

Subcellular location

Periplasm By similarity.

Sequence similarities

Belongs to the class-C beta-lactamase family.

Ontologies

Keywords
   Biological processAntibiotic resistance
   Cellular componentPeriplasm
   DomainSignal
   Molecular functionHydrolase
Gene Ontology (GO)
   Biological_processantibiotic catabolic process

Inferred from electronic annotation. Source: InterPro

response to antibiotic

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentouter membrane-bounded periplasmic space

Inferred from electronic annotation. Source: InterPro

   Molecular_functionbeta-lactamase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2020 By similarity
Chain21 – 385365Beta-lactamase
PRO_0000016960

Regions

Region335 – 3373Substrate binding By similarity

Sites

Active site841Acyl-ester intermediate By similarity
Active site1701Proton acceptor By similarity

Sequences

Sequence LengthMass (Da)Tools
Q48743 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 33DA95118FC2B207

FASTA38541,878
        10         20         30         40         50         60 
MKRLLAFCLL FFAALGQAKV PPPARSAADA EIQRAVAAFM QQYQVPGVAV GITVDGAERY 

        70         80         90        100        110        120 
YNYGVSSRKT QAKVGANTLF EVGSVSKTFT ATLASYAQVN QQLSLADHPG KYLPEMKGHD 

       130        140        150        160        170        180 
FDKVTLLNLG THTAGGFPMQ VPTQVKTDQQ LTAYFQSWHP QYPAGTKRTY ANPGIGMLGV 

       190        200        210        220        230        240 
IAAKSMRMPF QKAMTGVLLP KLGLTNTYLT VPPAKMAFYA QGYDDKGQPV RMSPGALWEP 

       250        260        270        280        290        300 
TYGIKTTARD LLRFVEINLD QVKVEPKLKR AIDGTHVGYY RLGEMTQGLV WEQLPYPASE 

       310        320        330        340        350        360 
TSLQANSSQK VIFESNAVAA LTPPRPPQAN VLINKTGSTR GFGAYVAFNP ARKIGIVLLM 

       370        380 
NRSVPMDGRI KLAHTILDTA GGMAK 

« Hide

References

[1]"Molecular analysis of the gene cluster involved in cephalosporin biosynthesis from Lysobacter lactamgenus YK90."
Kimura H., Izawa M., Sumino Y.
Appl. Microbiol. Biotechnol. 44:589-596(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: YK90.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X56660 Genomic DNA. Translation: CAA39987.1.
PIRS54103.

3D structure databases

ProteinModelPortalQ48743.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSS12.006.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.40.710.10. 1 hit.
InterProIPR001466. Beta-lactam-related.
IPR012338. Beta-lactam/transpept-like.
IPR001586. Beta-lactam_class-C_AS.
[Graphical view]
PfamPF00144. Beta-lactamase. 1 hit.
[Graphical view]
SUPFAMSSF56601. SSF56601. 1 hit.
PROSITEPS00336. BETA_LACTAMASE_C. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameAMPC_LYSLA
AccessionPrimary (citable) accession number: Q48743
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: October 16, 2013
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families