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Protein

Beta-lactamase

Gene
N/A
Organism
Lysobacter lactamgenus
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

This protein is a serine beta-lactamase with a substrate specificity for cephalosporins.

Catalytic activityi

A beta-lactam + H2O = a substituted beta-amino acid.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei84 – 841Acyl-ester intermediatePROSITE-ProRule annotation
Active sitei170 – 1701Proton acceptorBy similarity

GO - Molecular functioni

  1. beta-lactamase activity Source: UniProtKB-EC

GO - Biological processi

  1. antibiotic catabolic process Source: InterPro
  2. response to antibiotic Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Antibiotic resistance

Protein family/group databases

MEROPSiS12.006.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-lactamase (EC:3.5.2.6)
Alternative name(s):
Cephalosporinase
OrganismiLysobacter lactamgenus
Taxonomic identifieri39596 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaXanthomonadalesXanthomonadaceaeLysobacter

Subcellular locationi

Periplasm By similarity

GO - Cellular componenti

  1. outer membrane-bounded periplasmic space Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020By similarityAdd
BLAST
Chaini21 – 385365Beta-lactamasePRO_0000016960Add
BLAST

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni335 – 3373Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the class-C beta-lactamase family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR001466. Beta-lactam-related.
IPR012338. Beta-lactam/transpept-like.
IPR001586. Beta-lactam_class-C_AS.
[Graphical view]
PfamiPF00144. Beta-lactamase. 1 hit.
[Graphical view]
SUPFAMiSSF56601. SSF56601. 1 hit.
PROSITEiPS00336. BETA_LACTAMASE_C. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q48743-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRLLAFCLL FFAALGQAKV PPPARSAADA EIQRAVAAFM QQYQVPGVAV
60 70 80 90 100
GITVDGAERY YNYGVSSRKT QAKVGANTLF EVGSVSKTFT ATLASYAQVN
110 120 130 140 150
QQLSLADHPG KYLPEMKGHD FDKVTLLNLG THTAGGFPMQ VPTQVKTDQQ
160 170 180 190 200
LTAYFQSWHP QYPAGTKRTY ANPGIGMLGV IAAKSMRMPF QKAMTGVLLP
210 220 230 240 250
KLGLTNTYLT VPPAKMAFYA QGYDDKGQPV RMSPGALWEP TYGIKTTARD
260 270 280 290 300
LLRFVEINLD QVKVEPKLKR AIDGTHVGYY RLGEMTQGLV WEQLPYPASE
310 320 330 340 350
TSLQANSSQK VIFESNAVAA LTPPRPPQAN VLINKTGSTR GFGAYVAFNP
360 370 380
ARKIGIVLLM NRSVPMDGRI KLAHTILDTA GGMAK
Length:385
Mass (Da):41,878
Last modified:October 31, 1996 - v1
Checksum:i33DA95118FC2B207
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56660 Genomic DNA. Translation: CAA39987.1.
PIRiS54103.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56660 Genomic DNA. Translation: CAA39987.1.
PIRiS54103.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiS12.006.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR001466. Beta-lactam-related.
IPR012338. Beta-lactam/transpept-like.
IPR001586. Beta-lactam_class-C_AS.
[Graphical view]
PfamiPF00144. Beta-lactamase. 1 hit.
[Graphical view]
SUPFAMiSSF56601. SSF56601. 1 hit.
PROSITEiPS00336. BETA_LACTAMASE_C. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular analysis of the gene cluster involved in cephalosporin biosynthesis from Lysobacter lactamgenus YK90."
    Kimura H., Izawa M., Sumino Y.
    Appl. Microbiol. Biotechnol. 44:589-596(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: YK90.

Entry informationi

Entry nameiAMPC_LYSLA
AccessioniPrimary (citable) accession number: Q48743
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2000
Last sequence update: October 31, 1996
Last modified: March 31, 2015
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.