Reviewed, UniProtKB/Swiss-Prot Q48743 (AMPC_LYSLA)

Names and origin

Protein names	Recommended name:     Beta-lactamase     EC=3.5.2.6 Alternative name(s):     Cephalosporinase
Organism	Lysobacter lactamgenus
Taxonomic identifier	39596 [NCBI]
Taxonomic lineage	Bacteria › Proteobacteria › Gammaproteobacteria › Xanthomonadales › Xanthomonadaceae › Lysobacter

Protein attributes

Sequence length	385 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This protein is a serine beta-lactamase with a substrate specificity for cephalosporins.
Catalytic activity	A beta-lactam + H2O = a substituted beta-amino acid.
Subcellular location	Periplasm By similarity.
Sequence similarities	Belongs to the class-C beta-lactamase family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Cellular component	Periplasm
Domain	Signal
Molecular function	Hydrolase
Gene Ontology (GO)
Biological process	antibiotic catabolic process Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	outer membrane-bounded periplasmic space Inferred from electronic annotation. Source: InterPro
Molecular function	beta-lactamase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	By similarity
	Chain	21 – 385	365	Beta-lactamase		PRO_0000016960
Regions
	Region	335 – 337	3	Substrate binding By similarity
Sites
	Active site	84	1	Acyl-ester intermediate By similarity
	Active site	170	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q48743-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 33DA95118FC2B207	FASTA	385	41,878
	10 20 30 40 50 60 MKRLLAFCLL FFAALGQAKV PPPARSAADA EIQRAVAAFM QQYQVPGVAV GITVDGAERY 70 80 90 100 110 120 YNYGVSSRKT QAKVGANTLF EVGSVSKTFT ATLASYAQVN QQLSLADHPG KYLPEMKGHD 130 140 150 160 170 180 FDKVTLLNLG THTAGGFPMQ VPTQVKTDQQ LTAYFQSWHP QYPAGTKRTY ANPGIGMLGV 190 200 210 220 230 240 IAAKSMRMPF QKAMTGVLLP KLGLTNTYLT VPPAKMAFYA QGYDDKGQPV RMSPGALWEP 250 260 270 280 290 300 TYGIKTTARD LLRFVEINLD QVKVEPKLKR AIDGTHVGYY RLGEMTQGLV WEQLPYPASE 310 320 330 340 350 360 TSLQANSSQK VIFESNAVAA LTPPRPPQAN VLINKTGSTR GFGAYVAFNP ARKIGIVLLM 370 380 NRSVPMDGRI KLAHTILDTA GGMAK

References

[1]	"Molecular analysis of the gene cluster involved in cephalosporin biosynthesis from Lysobacter lactamgenus YK90." Kimura H., Izawa M., Sumino Y. Appl. Microbiol. Biotechnol. 44:589-596(1996) [PubMed: 8703429] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: YK90.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X56660 Genomic DNA. Translation: CAA39987.1.
PIR	S54103.
3D structure databases
HSSP	HSSP built from PDB template 1GA9 based on UniProtKB P00811.
ModBase	Search...
Protein family/group databases
MEROPS	S12.006.
Enzyme and pathway databases
BRENDA	3.5.2.6. 261148.
Family and domain databases
InterPro	IPR001586. Beta_lactam_C_AS. IPR001466. Beta_lactamase-related. [Graphical view]
Pfam	PF00144. Beta-lactamase. 1 hit. [Graphical view]
PROSITE	PS00336. BETA_LACTAMASE_C. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPC_LYSLA

Accession

Primary (citable) accession number: Q48743

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)