Q48739 (IPNS_LYSLA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Isopenicillin N synthase
Short name=IPNS
EC=1.21.3.1

Gene names

Name:

pcbC

Organism

Lysobacter lactamgenus

Taxonomic identifier

39596 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Xanthomonadales › Xanthomonadaceae › Lysobacter

Protein attributes

Sequence length	326 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Removes, in the presence of oxygen, 4 hydrogen atoms from delta-L-(alpha-aminoadipyl)-L-cysteinyl-D-valine (ACV) to form the azetidinone and thiazolidine rings of isopenicillin.
Catalytic activity	N-((5S)-5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine + O2 = isopenicillin N + 2 H2O.
Cofactor	Iron. Ascorbate.
Pathway	Antibiotic biosynthesis; penicillin G biosynthesis; penicillin G from L-alpha-aminoadipate and L-cysteine and L-valine: step 2/3.
Sequence similarities	Belongs to the iron/ascorbate-dependent oxidoreductase family. Contains 1 Fe2OG dioxygenase domain.

Ontologies

Keywords
Biological process	Antibiotic biosynthesis
Ligand	Iron Metal-binding Vitamin C
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	antibiotic biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	L-ascorbic acid binding Inferred from electronic annotation. Source: UniProtKB-KW iron ion binding Inferred from electronic annotation. Source: InterPro isopenicillin-N synthase activity Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 326	326	Isopenicillin N synthase		PRO_0000219501
Regions
	Domain	183 – 283	101	Fe2OG dioxygenase
Sites
	Metal binding	209	1	Iron By similarity
	Metal binding	211	1	Iron By similarity
	Metal binding	265	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q48739 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 25731F67173F8447	FASTA	326	36,564
	10 20 30 40 50 60 MNRHADVPVI DISGLSGNDM DVKKDIAARI DRACRGSGFF YAANHGVDLA ALQKFTTDWH 70 80 90 100 110 120 MAMSPEEKWE LAIRAYNPAN PRNRNGYYMA VEGKKANESF CYLNPSFDAD HATIKAGLPS 130 140 150 160 170 180 HEVNIWPDEA RHPGMRRFYE AYFSDVFDVA AVILRGFAIA LGREESFFER HFSMDDTLSA 190 200 210 220 230 240 VSLIRYPFLE NYPPLKLGPD GEKLSFEHHQ DVSLITVLYQ TAIPNLQVET AEGYLDIPVS 250 260 270 280 290 300 DEHFLVNCGT YMAHITNGYY PAPVHRVKYI NAERLSIPFF ANLSHASAID PFAPPPYAPA 310 320 RGNPTVSYGD YLQHGLLDLI RANGQT

References

[1]	"Molecular analysis of the gene cluster involved in cephalosporin biosynthesis from Lysobacter lactamgenus YK90." Kimura H., Izawa M., Sumino Y. Appl. Microbiol. Biotechnol. 44:589-596(1996) [PubMed: 8703429] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: YK90.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X56660 Genomic DNA. Translation: CAA39983.1.
PIR	S54099.
3D structure databases
ProteinModelPortal	Q48739.
SMR	Q48739. Positions 4-326.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR002057. Isopenicillin-N_synth_CS. IPR002283. Isopenicillin-N_synthase. IPR005123. Oxoglutarate/Fe-dep_oxygenase. [Graphical view]
Pfam	PF03171. 2OG-FeII_Oxy. 1 hit. [Graphical view]
PRINTS	PR00682. IPNSYNTHASE.
PROSITE	PS51471. FE2OG_OXY. 1 hit. PS00185. IPNS_1. 1 hit. PS00186. IPNS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IPNS_LYSLA

Accession

Primary (citable) accession number: Q48739

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	November 1, 1996
Last modified:	October 19, 2011
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program