Reviewed, UniProtKB/Swiss-Prot Q48739 (IPNS_LYSLA)

Names and origin

Protein names

Recommended name:
    Isopenicillin N synthetase
    EC=1.21.3.1
Alternative name(s):
    Isopenicillin N synthase
      Short name=IPNS

Gene names

Name:

pcbC

Organism

Lysobacter lactamgenus

Taxonomic identifier

39596 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Xanthomonadales › Xanthomonadaceae › Lysobacter

Protein attributes

Sequence length	326 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Removes, in the presence of oxygen, 4 hydrogen atoms from delta-L-(alpha-aminoadipyl)-L-cysteinyl-D-valine (ACV) to form the azetidinone and thiazolidine rings of isopenicillin.
Catalytic activity	N-((5S)-5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine + O2 = isopenicillin N + 2 H2O.
Cofactor	Iron. Ascorbate.
Pathway	Antibiotic biosynthesis; penicillin G biosynthesis; penicillin G from L-alpha-aminoadipate and L-cysteine and L-valine: step 2/3.
Sequence similarities	Belongs to the iron/ascorbate-dependent oxidoreductase family.

Ontologies

Keywords
Biological process	Antibiotic biosynthesis
Ligand	Iron Metal-binding Vitamin C
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	antibiotic biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	L-ascorbic acid binding Inferred from electronic annotation. Source: UniProtKB-KW iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW isopenicillin-N synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 326	326	Isopenicillin N synthetase		PRO_0000219501
Sites
	Metal binding	209	1	Iron By similarity
	Metal binding	211	1	Iron By similarity
	Metal binding	265	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q48739-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 25731F67173F8447	FASTA	326	36,564
	10 20 30 40 50 60 MNRHADVPVI DISGLSGNDM DVKKDIAARI DRACRGSGFF YAANHGVDLA ALQKFTTDWH 70 80 90 100 110 120 MAMSPEEKWE LAIRAYNPAN PRNRNGYYMA VEGKKANESF CYLNPSFDAD HATIKAGLPS 130 140 150 160 170 180 HEVNIWPDEA RHPGMRRFYE AYFSDVFDVA AVILRGFAIA LGREESFFER HFSMDDTLSA 190 200 210 220 230 240 VSLIRYPFLE NYPPLKLGPD GEKLSFEHHQ DVSLITVLYQ TAIPNLQVET AEGYLDIPVS 250 260 270 280 290 300 DEHFLVNCGT YMAHITNGYY PAPVHRVKYI NAERLSIPFF ANLSHASAID PFAPPPYAPA 310 320 RGNPTVSYGD YLQHGLLDLI RANGQT

References

[1]	"Molecular analysis of the gene cluster involved in cephalosporin biosynthesis from Lysobacter lactamgenus YK90." Kimura H., Izawa M., Sumino Y. Appl. Microbiol. Biotechnol. 44:589-596(1996) [PubMed: 8703429] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: YK90.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X56660 Genomic DNA. Translation: CAA39983.1.
PIR	S54099.
3D structure databases
HSSP	HSSP built from PDB template 1ODM based on UniProtKB P05326.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.21.3.1. 261148.
Family and domain databases
InterPro	IPR002057. Isopenicillin-N_synth_CS. IPR002283. Isopenicillin-N_synthase. IPR005123. Oxoglutarate/Fe-dep_Oase. [Graphical view]
Pfam	PF03171. 2OG-FeII_Oxy. 1 hit. [Graphical view]
PRINTS	PR00682. IPNSYNTHASE.
PROSITE	PS00185. IPNS_1. 1 hit. PS00186. IPNS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IPNS_LYSLA

Accession

Primary (citable) accession number: Q48739

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)