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Q48727

- BGAL_LACLA

UniProt

Q48727 - BGAL_LACLA

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Protein
Beta-galactosidase
Gene
lacZ, LL1980, L0025
Organism
Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei431 – 4311Proton donor By similarity
Active sitei508 – 5081Nucleophile By similarity

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC
  2. carbohydrate binding Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BioCyciLLAC272623:GHSH-2108-MONOMER.
SABIO-RKQ48727.

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase (EC:3.2.1.23)
Alternative name(s):
Lactase
Gene namesi
Name:lacZ
Ordered Locus Names:LL1980
ORF Names:L0025
OrganismiLactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)
Taxonomic identifieri272623 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus
ProteomesiUP000002196: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. beta-galactosidase complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 998998Beta-galactosidase
PRO_0000057669Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi272623.L0025.

Structurei

3D structure databases

ProteinModelPortaliQ48727.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG3250.
HOGENOMiHOG000252443.
KOiK01190.
OMAiNPPFVPK.
OrthoDBiEOG6XWV0T.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q48727-1 [UniParc]FASTAAdd to Basket

« Hide

MAMMTMIDVL ERKDWENPVV SNWNRLPMHT PMDLLEKQSL NGLWNFDHFS    50
RISDVPKNWL ELTESKTEII VPSNWQIEFK DKSDVPIYTN VTYPIPIQPP 100
YVPEANPVGA YSRYFDITKE WLESGHVHLT FEGVGSAFHF WLNGEYGGYS 150
EDSRLPAEFD ISNLAKEGQN CLKVLVFRWS KVTYFEDQDM WRMSGIFRSV 200
NLQWLPDNYL LDFSIKTDLD EDLDFANVKL QAYAKNIDDA CLEFKLYDDE 250
QLIGECHGFD AEIGVVNPKL WSDEIPYLYR LELTLMDRSG AVFHKETKKI 300
GIRKIAIEKG QLKINGKALL VRGVNKHEFT PEHGYVVSEE VMIKDIKLMK 350
EHNFNAVRCS HYPNDSRWYE LCDEYGLYVM DEANIETHGM TPMNRLTNDP 400
TYLPLMSERV TRMVMRDRNH PSIIIWSLGN ESGYGSNHQA LYDWCKSFDS 450
SRPVHYEGGD DASRGATDAT DIICPMYARV DSPSINAPYS LKTWMGVAGE 500
NRPLILCEYA HDMGNSLGGF GKYWQAFREI DRLQGGFIWD WVDQGLLKDG 550
NYAYGGDFGD KPNDRQFSLN GLVFPNRQAK PALREAKYWQ QYYQFELEKT 600
PLGQVFAFTV TNEYLFRSTD NEKLCYQLIN GLEVLWENEL ILNMPAGGSM 650
RIDLSELPID GTDNLFLNIQ VKTIEKCNLL ESDFEVAHQQ FVLQEKINFT 700
DRIDSNEEIT LFEDEELLTV RSAKQKFIFN KSNGNLSRWL DEKGNEKLLH 750
ELSEQFTRAP LDNDIGVSEV EHIDPNAWLE RWKGIGFYEL KTLLKTMIIQ 800
ATENEVIISV QTDYEAKGKI AFSTIREYHI FRNGELLLKV DFKRNIEFPE 850
PARIGLSLQL AEKAENVTYF GLGPDENYPD RRGASLFGQW NLRITDMTTP 900
YIFPSENGLR METRELNYDR LKVRAMGQSF AFNLSPYSQN QLAKKGHWHL 950
LEEEAGTWLN IDGFHMGVGG DDSWSPSVAQ EYLLTKGNYH YEVSFKLT 998
Length:998
Mass (Da):115,440
Last modified:September 26, 2001 - v3
Checksum:i8989FD4287ADDA89
GO

Sequence cautioni

The sequence AAK06078.1 differs from that shown. Reason: Erroneous initiation.
The sequence CAA56341.1 differs from that shown. Reason: Erroneous initiation.

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti34 – 341L → F in strain: ATCC 7962.
Natural varianti182 – 1821V → G in strain: ATCC 7962.
Natural varianti237 – 2371I → M in strain: ATCC 7962.
Natural varianti629 – 6291I → T in strain: ATCC 7962.
Natural varianti677 – 6771C → Y in strain: ATCC 7962.
Natural varianti702 – 7021R → K in strain: ATCC 7962.
Natural varianti712 – 7121F → L in strain: ATCC 7962.
Natural varianti784 – 7852GI → AV in strain: ATCC 7962.
Natural varianti796 – 7961T → N in strain: ATCC 7962.
Natural varianti919 – 9191D → G in strain: ATCC 7962.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U60828 Genomic DNA. Translation: AAD11504.1.
X80037 Genomic DNA. Translation: CAA56341.1. Different initiation.
AE005176 Genomic DNA. Translation: AAK06078.1. Different initiation.
PIRiD86872.
RefSeqiNP_268137.2. NC_002662.1.

Genome annotation databases

EnsemblBacteriaiAAK06078; AAK06078; L0025.
GeneIDi1115654.
KEGGilla:L0025.
PATRICi22296478. VBILacLac136773_2132.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U60828 Genomic DNA. Translation: AAD11504.1 .
X80037 Genomic DNA. Translation: CAA56341.1 . Different initiation.
AE005176 Genomic DNA. Translation: AAK06078.1 . Different initiation.
PIRi D86872.
RefSeqi NP_268137.2. NC_002662.1.

3D structure databases

ProteinModelPortali Q48727.
ModBasei Search...

Protein-protein interaction databases

STRINGi 272623.L0025.

Protein family/group databases

CAZyi GH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAK06078 ; AAK06078 ; L0025 .
GeneIDi 1115654.
KEGGi lla:L0025.
PATRICi 22296478. VBILacLac136773_2132.

Phylogenomic databases

eggNOGi COG3250.
HOGENOMi HOG000252443.
KOi K01190.
OMAi NPPFVPK.
OrthoDBi EOG6XWV0T.

Enzyme and pathway databases

BioCyci LLAC272623:GHSH-2108-MONOMER.
SABIO-RK Q48727.

Family and domain databases

Gene3Di 2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
InterProi IPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view ]
PRINTSi PR00132. GLHYDRLASE2.
SMARTi SM01038. Bgal_small_N. 1 hit.
[Graphical view ]
SUPFAMi SSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEi PS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and nucleotide sequence of the beta-galactosidase gene from Lactococcus lactis ssp. lactis ATCC7962."
    Lee J.M., Park J.H., Lee W.K., Chang H.C., Kim J.H., Chung D.K., Lee H.J.
    Biotechnol. Lett. 19:179-183(1997)
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 7962.
  2. "Cloning, DNA sequence, and regulation of expression of a gene encoding beta-galactosidase from Lactococcus lactis."
    Griffin H.G., Maccormick C.A., Gasson M.J.
    DNA Seq. 6:337-346(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 7962.
  3. "The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403."
    Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J., Ehrlich S.D., Sorokin A.
    Genome Res. 11:731-753(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: IL1403.

Entry informationi

Entry nameiBGAL_LACLA
AccessioniPrimary (citable) accession number: Q48727
Secondary accession number(s): P71448, Q9CE65
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: September 26, 2001
Last modified: May 14, 2014
This is version 101 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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