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Q48727

- BGAL_LACLA

UniProt

Q48727 - BGAL_LACLA

Protein

Beta-galactosidase

Gene

lacZ

Organism
Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 102 (01 Oct 2014)
      Sequence version 3 (26 Sep 2001)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei431 – 4311Proton donorBy similarity
    Active sitei508 – 5081NucleophileBy similarity

    GO - Molecular functioni

    1. beta-galactosidase activity Source: UniProtKB-EC
    2. carbohydrate binding Source: InterPro

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Enzyme and pathway databases

    BioCyciLLAC272623:GHSH-2108-MONOMER.
    SABIO-RKQ48727.

    Protein family/group databases

    CAZyiGH2. Glycoside Hydrolase Family 2.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-galactosidase (EC:3.2.1.23)
    Alternative name(s):
    Lactase
    Gene namesi
    Name:lacZ
    Ordered Locus Names:LL1980
    ORF Names:L0025
    OrganismiLactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)
    Taxonomic identifieri272623 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus
    ProteomesiUP000002196: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. beta-galactosidase complex Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 998998Beta-galactosidasePRO_0000057669Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi272623.L0025.

    Structurei

    3D structure databases

    ProteinModelPortaliQ48727.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 2 family.Curated

    Phylogenomic databases

    eggNOGiCOG3250.
    HOGENOMiHOG000252443.
    KOiK01190.
    OMAiNPPFVPK.
    OrthoDBiEOG6XWV0T.

    Family and domain databases

    Gene3Di2.60.120.260. 1 hit.
    2.60.40.320. 2 hits.
    2.70.98.10. 1 hit.
    3.20.20.80. 1 hit.
    InterProiIPR004199. B-gal_small/dom_5.
    IPR011013. Gal_mutarotase_SF_dom.
    IPR008979. Galactose-bd-like.
    IPR014718. Glyco_hydro-type_carb-bd_sub.
    IPR006101. Glyco_hydro_2.
    IPR013812. Glyco_hydro_2/20_Ig-like.
    IPR023232. Glyco_hydro_2_AS.
    IPR023230. Glyco_hydro_2_CS.
    IPR006102. Glyco_hydro_2_Ig-like.
    IPR006104. Glyco_hydro_2_N.
    IPR006103. Glyco_hydro_2_TIM.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF02929. Bgal_small_N. 1 hit.
    PF00703. Glyco_hydro_2. 1 hit.
    PF02836. Glyco_hydro_2_C. 1 hit.
    PF02837. Glyco_hydro_2_N. 1 hit.
    [Graphical view]
    PRINTSiPR00132. GLHYDRLASE2.
    SMARTiSM01038. Bgal_small_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF49303. SSF49303. 2 hits.
    SSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.
    SSF74650. SSF74650. 1 hit.
    PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
    PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q48727-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAMMTMIDVL ERKDWENPVV SNWNRLPMHT PMDLLEKQSL NGLWNFDHFS    50
    RISDVPKNWL ELTESKTEII VPSNWQIEFK DKSDVPIYTN VTYPIPIQPP 100
    YVPEANPVGA YSRYFDITKE WLESGHVHLT FEGVGSAFHF WLNGEYGGYS 150
    EDSRLPAEFD ISNLAKEGQN CLKVLVFRWS KVTYFEDQDM WRMSGIFRSV 200
    NLQWLPDNYL LDFSIKTDLD EDLDFANVKL QAYAKNIDDA CLEFKLYDDE 250
    QLIGECHGFD AEIGVVNPKL WSDEIPYLYR LELTLMDRSG AVFHKETKKI 300
    GIRKIAIEKG QLKINGKALL VRGVNKHEFT PEHGYVVSEE VMIKDIKLMK 350
    EHNFNAVRCS HYPNDSRWYE LCDEYGLYVM DEANIETHGM TPMNRLTNDP 400
    TYLPLMSERV TRMVMRDRNH PSIIIWSLGN ESGYGSNHQA LYDWCKSFDS 450
    SRPVHYEGGD DASRGATDAT DIICPMYARV DSPSINAPYS LKTWMGVAGE 500
    NRPLILCEYA HDMGNSLGGF GKYWQAFREI DRLQGGFIWD WVDQGLLKDG 550
    NYAYGGDFGD KPNDRQFSLN GLVFPNRQAK PALREAKYWQ QYYQFELEKT 600
    PLGQVFAFTV TNEYLFRSTD NEKLCYQLIN GLEVLWENEL ILNMPAGGSM 650
    RIDLSELPID GTDNLFLNIQ VKTIEKCNLL ESDFEVAHQQ FVLQEKINFT 700
    DRIDSNEEIT LFEDEELLTV RSAKQKFIFN KSNGNLSRWL DEKGNEKLLH 750
    ELSEQFTRAP LDNDIGVSEV EHIDPNAWLE RWKGIGFYEL KTLLKTMIIQ 800
    ATENEVIISV QTDYEAKGKI AFSTIREYHI FRNGELLLKV DFKRNIEFPE 850
    PARIGLSLQL AEKAENVTYF GLGPDENYPD RRGASLFGQW NLRITDMTTP 900
    YIFPSENGLR METRELNYDR LKVRAMGQSF AFNLSPYSQN QLAKKGHWHL 950
    LEEEAGTWLN IDGFHMGVGG DDSWSPSVAQ EYLLTKGNYH YEVSFKLT 998
    Length:998
    Mass (Da):115,440
    Last modified:September 26, 2001 - v3
    Checksum:i8989FD4287ADDA89
    GO

    Sequence cautioni

    The sequence AAK06078.1 differs from that shown. Reason: Erroneous initiation.
    The sequence CAA56341.1 differs from that shown. Reason: Erroneous initiation.

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti34 – 341L → F in strain: ATCC 7962.
    Natural varianti182 – 1821V → G in strain: ATCC 7962.
    Natural varianti237 – 2371I → M in strain: ATCC 7962.
    Natural varianti629 – 6291I → T in strain: ATCC 7962.
    Natural varianti677 – 6771C → Y in strain: ATCC 7962.
    Natural varianti702 – 7021R → K in strain: ATCC 7962.
    Natural varianti712 – 7121F → L in strain: ATCC 7962.
    Natural varianti784 – 7852GI → AV in strain: ATCC 7962.
    Natural varianti796 – 7961T → N in strain: ATCC 7962.
    Natural varianti919 – 9191D → G in strain: ATCC 7962.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U60828 Genomic DNA. Translation: AAD11504.1.
    X80037 Genomic DNA. Translation: CAA56341.1. Different initiation.
    AE005176 Genomic DNA. Translation: AAK06078.1. Different initiation.
    PIRiD86872.
    RefSeqiNP_268137.2. NC_002662.1.

    Genome annotation databases

    EnsemblBacteriaiAAK06078; AAK06078; L0025.
    GeneIDi1115654.
    KEGGilla:L0025.
    PATRICi22296478. VBILacLac136773_2132.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U60828 Genomic DNA. Translation: AAD11504.1 .
    X80037 Genomic DNA. Translation: CAA56341.1 . Different initiation.
    AE005176 Genomic DNA. Translation: AAK06078.1 . Different initiation.
    PIRi D86872.
    RefSeqi NP_268137.2. NC_002662.1.

    3D structure databases

    ProteinModelPortali Q48727.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 272623.L0025.

    Protein family/group databases

    CAZyi GH2. Glycoside Hydrolase Family 2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAK06078 ; AAK06078 ; L0025 .
    GeneIDi 1115654.
    KEGGi lla:L0025.
    PATRICi 22296478. VBILacLac136773_2132.

    Phylogenomic databases

    eggNOGi COG3250.
    HOGENOMi HOG000252443.
    KOi K01190.
    OMAi NPPFVPK.
    OrthoDBi EOG6XWV0T.

    Enzyme and pathway databases

    BioCyci LLAC272623:GHSH-2108-MONOMER.
    SABIO-RK Q48727.

    Family and domain databases

    Gene3Di 2.60.120.260. 1 hit.
    2.60.40.320. 2 hits.
    2.70.98.10. 1 hit.
    3.20.20.80. 1 hit.
    InterProi IPR004199. B-gal_small/dom_5.
    IPR011013. Gal_mutarotase_SF_dom.
    IPR008979. Galactose-bd-like.
    IPR014718. Glyco_hydro-type_carb-bd_sub.
    IPR006101. Glyco_hydro_2.
    IPR013812. Glyco_hydro_2/20_Ig-like.
    IPR023232. Glyco_hydro_2_AS.
    IPR023230. Glyco_hydro_2_CS.
    IPR006102. Glyco_hydro_2_Ig-like.
    IPR006104. Glyco_hydro_2_N.
    IPR006103. Glyco_hydro_2_TIM.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF02929. Bgal_small_N. 1 hit.
    PF00703. Glyco_hydro_2. 1 hit.
    PF02836. Glyco_hydro_2_C. 1 hit.
    PF02837. Glyco_hydro_2_N. 1 hit.
    [Graphical view ]
    PRINTSi PR00132. GLHYDRLASE2.
    SMARTi SM01038. Bgal_small_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49303. SSF49303. 2 hits.
    SSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.
    SSF74650. SSF74650. 1 hit.
    PROSITEi PS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
    PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and nucleotide sequence of the beta-galactosidase gene from Lactococcus lactis ssp. lactis ATCC7962."
      Lee J.M., Park J.H., Lee W.K., Chang H.C., Kim J.H., Chung D.K., Lee H.J.
      Biotechnol. Lett. 19:179-183(1997)
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 7962.
    2. "Cloning, DNA sequence, and regulation of expression of a gene encoding beta-galactosidase from Lactococcus lactis."
      Griffin H.G., Maccormick C.A., Gasson M.J.
      DNA Seq. 6:337-346(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 7962.
    3. "The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403."
      Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J., Ehrlich S.D., Sorokin A.
      Genome Res. 11:731-753(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: IL1403.

    Entry informationi

    Entry nameiBGAL_LACLA
    AccessioniPrimary (citable) accession number: Q48727
    Secondary accession number(s): P71448, Q9CE65
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: September 26, 2001
    Last modified: October 1, 2014
    This is version 102 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3