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Q48727

- BGAL_LACLA

UniProt

Q48727 - BGAL_LACLA

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Protein

Beta-galactosidase

Gene

lacZ

Organism
Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei431 – 4311Proton donorBy similarity
Active sitei508 – 5081NucleophileBy similarity

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC
  2. carbohydrate binding Source: InterPro

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BioCyciLLAC272623:GHSH-2108-MONOMER.
SABIO-RKQ48727.

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase (EC:3.2.1.23)
Alternative name(s):
Lactase
Gene namesi
Name:lacZ
Ordered Locus Names:LL1980
ORF Names:L0025
OrganismiLactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)
Taxonomic identifieri272623 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus
ProteomesiUP000002196: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. beta-galactosidase complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 998998Beta-galactosidasePRO_0000057669Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi272623.L0025.

Structurei

3D structure databases

ProteinModelPortaliQ48727.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 2 family.Curated

Phylogenomic databases

eggNOGiCOG3250.
HOGENOMiHOG000252443.
KOiK01190.
OMAiNPPFVPK.
OrthoDBiEOG6XWV0T.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q48727-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAMMTMIDVL ERKDWENPVV SNWNRLPMHT PMDLLEKQSL NGLWNFDHFS
60 70 80 90 100
RISDVPKNWL ELTESKTEII VPSNWQIEFK DKSDVPIYTN VTYPIPIQPP
110 120 130 140 150
YVPEANPVGA YSRYFDITKE WLESGHVHLT FEGVGSAFHF WLNGEYGGYS
160 170 180 190 200
EDSRLPAEFD ISNLAKEGQN CLKVLVFRWS KVTYFEDQDM WRMSGIFRSV
210 220 230 240 250
NLQWLPDNYL LDFSIKTDLD EDLDFANVKL QAYAKNIDDA CLEFKLYDDE
260 270 280 290 300
QLIGECHGFD AEIGVVNPKL WSDEIPYLYR LELTLMDRSG AVFHKETKKI
310 320 330 340 350
GIRKIAIEKG QLKINGKALL VRGVNKHEFT PEHGYVVSEE VMIKDIKLMK
360 370 380 390 400
EHNFNAVRCS HYPNDSRWYE LCDEYGLYVM DEANIETHGM TPMNRLTNDP
410 420 430 440 450
TYLPLMSERV TRMVMRDRNH PSIIIWSLGN ESGYGSNHQA LYDWCKSFDS
460 470 480 490 500
SRPVHYEGGD DASRGATDAT DIICPMYARV DSPSINAPYS LKTWMGVAGE
510 520 530 540 550
NRPLILCEYA HDMGNSLGGF GKYWQAFREI DRLQGGFIWD WVDQGLLKDG
560 570 580 590 600
NYAYGGDFGD KPNDRQFSLN GLVFPNRQAK PALREAKYWQ QYYQFELEKT
610 620 630 640 650
PLGQVFAFTV TNEYLFRSTD NEKLCYQLIN GLEVLWENEL ILNMPAGGSM
660 670 680 690 700
RIDLSELPID GTDNLFLNIQ VKTIEKCNLL ESDFEVAHQQ FVLQEKINFT
710 720 730 740 750
DRIDSNEEIT LFEDEELLTV RSAKQKFIFN KSNGNLSRWL DEKGNEKLLH
760 770 780 790 800
ELSEQFTRAP LDNDIGVSEV EHIDPNAWLE RWKGIGFYEL KTLLKTMIIQ
810 820 830 840 850
ATENEVIISV QTDYEAKGKI AFSTIREYHI FRNGELLLKV DFKRNIEFPE
860 870 880 890 900
PARIGLSLQL AEKAENVTYF GLGPDENYPD RRGASLFGQW NLRITDMTTP
910 920 930 940 950
YIFPSENGLR METRELNYDR LKVRAMGQSF AFNLSPYSQN QLAKKGHWHL
960 970 980 990
LEEEAGTWLN IDGFHMGVGG DDSWSPSVAQ EYLLTKGNYH YEVSFKLT
Length:998
Mass (Da):115,440
Last modified:September 26, 2001 - v3
Checksum:i8989FD4287ADDA89
GO

Sequence cautioni

The sequence AAK06078.1 differs from that shown. Reason: Erroneous initiation.
The sequence CAA56341.1 differs from that shown. Reason: Erroneous initiation.

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti34 – 341L → F in strain: ATCC 7962.
Natural varianti182 – 1821V → G in strain: ATCC 7962.
Natural varianti237 – 2371I → M in strain: ATCC 7962.
Natural varianti629 – 6291I → T in strain: ATCC 7962.
Natural varianti677 – 6771C → Y in strain: ATCC 7962.
Natural varianti702 – 7021R → K in strain: ATCC 7962.
Natural varianti712 – 7121F → L in strain: ATCC 7962.
Natural varianti784 – 7852GI → AV in strain: ATCC 7962.
Natural varianti796 – 7961T → N in strain: ATCC 7962.
Natural varianti919 – 9191D → G in strain: ATCC 7962.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U60828 Genomic DNA. Translation: AAD11504.1.
X80037 Genomic DNA. Translation: CAA56341.1. Different initiation.
AE005176 Genomic DNA. Translation: AAK06078.1. Different initiation.
PIRiD86872.
RefSeqiNP_268137.2. NC_002662.1.

Genome annotation databases

EnsemblBacteriaiAAK06078; AAK06078; L0025.
GeneIDi1115654.
KEGGilla:L0025.
PATRICi22296478. VBILacLac136773_2132.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U60828 Genomic DNA. Translation: AAD11504.1 .
X80037 Genomic DNA. Translation: CAA56341.1 . Different initiation.
AE005176 Genomic DNA. Translation: AAK06078.1 . Different initiation.
PIRi D86872.
RefSeqi NP_268137.2. NC_002662.1.

3D structure databases

ProteinModelPortali Q48727.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 272623.L0025.

Protein family/group databases

CAZyi GH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAK06078 ; AAK06078 ; L0025 .
GeneIDi 1115654.
KEGGi lla:L0025.
PATRICi 22296478. VBILacLac136773_2132.

Phylogenomic databases

eggNOGi COG3250.
HOGENOMi HOG000252443.
KOi K01190.
OMAi NPPFVPK.
OrthoDBi EOG6XWV0T.

Enzyme and pathway databases

BioCyci LLAC272623:GHSH-2108-MONOMER.
SABIO-RK Q48727.

Family and domain databases

Gene3Di 2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
InterProi IPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view ]
PRINTSi PR00132. GLHYDRLASE2.
SMARTi SM01038. Bgal_small_N. 1 hit.
[Graphical view ]
SUPFAMi SSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEi PS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and nucleotide sequence of the beta-galactosidase gene from Lactococcus lactis ssp. lactis ATCC7962."
    Lee J.M., Park J.H., Lee W.K., Chang H.C., Kim J.H., Chung D.K., Lee H.J.
    Biotechnol. Lett. 19:179-183(1997)
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 7962.
  2. "Cloning, DNA sequence, and regulation of expression of a gene encoding beta-galactosidase from Lactococcus lactis."
    Griffin H.G., Maccormick C.A., Gasson M.J.
    DNA Seq. 6:337-346(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 7962.
  3. "The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403."
    Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J., Ehrlich S.D., Sorokin A.
    Genome Res. 11:731-753(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: IL1403.

Entry informationi

Entry nameiBGAL_LACLA
AccessioniPrimary (citable) accession number: Q48727
Secondary accession number(s): P71448, Q9CE65
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: September 26, 2001
Last modified: October 1, 2014
This is version 102 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3