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Q48727 (BGAL_LACLA) Reviewed, UniProtKB/Swiss-Prot

Last modified November 13, 2013. Version 100. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-galactosidase

EC=3.2.1.23
Alternative name(s):
Lactase
Gene names
Name:lacZ
Ordered Locus Names:LL1980
ORF Names:L0025
OrganismLactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis) [Reference proteome] [HAMAP]
Taxonomic identifier272623 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus

Protein attributes

Sequence length998 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Sequence similarities

Belongs to the glycosyl hydrolase 2 family.

Sequence caution

The sequence AAK06078.1 differs from that shown. Reason: Erroneous initiation.

The sequence CAA56341.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Molecular functionGlycosidase
Hydrolase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentbeta-galactosidase complex

Inferred from electronic annotation. Source: InterPro

   Molecular_functionbeta-galactosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

carbohydrate binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 998998Beta-galactosidase
PRO_0000057669

Sites

Active site4311Proton donor By similarity
Active site5081Nucleophile By similarity

Natural variations

Natural variant341L → F in strain: ATCC 7962.
Natural variant1821V → G in strain: ATCC 7962.
Natural variant2371I → M in strain: ATCC 7962.
Natural variant6291I → T in strain: ATCC 7962.
Natural variant6771C → Y in strain: ATCC 7962.
Natural variant7021R → K in strain: ATCC 7962.
Natural variant7121F → L in strain: ATCC 7962.
Natural variant784 – 7852GI → AV in strain: ATCC 7962.
Natural variant7961T → N in strain: ATCC 7962.
Natural variant9191D → G in strain: ATCC 7962.

Sequences

Sequence LengthMass (Da)Tools
Q48727 [UniParc].

Last modified September 26, 2001. Version 3.
Checksum: 8989FD4287ADDA89

FASTA998115,440
        10         20         30         40         50         60 
MAMMTMIDVL ERKDWENPVV SNWNRLPMHT PMDLLEKQSL NGLWNFDHFS RISDVPKNWL 

        70         80         90        100        110        120 
ELTESKTEII VPSNWQIEFK DKSDVPIYTN VTYPIPIQPP YVPEANPVGA YSRYFDITKE 

       130        140        150        160        170        180 
WLESGHVHLT FEGVGSAFHF WLNGEYGGYS EDSRLPAEFD ISNLAKEGQN CLKVLVFRWS 

       190        200        210        220        230        240 
KVTYFEDQDM WRMSGIFRSV NLQWLPDNYL LDFSIKTDLD EDLDFANVKL QAYAKNIDDA 

       250        260        270        280        290        300 
CLEFKLYDDE QLIGECHGFD AEIGVVNPKL WSDEIPYLYR LELTLMDRSG AVFHKETKKI 

       310        320        330        340        350        360 
GIRKIAIEKG QLKINGKALL VRGVNKHEFT PEHGYVVSEE VMIKDIKLMK EHNFNAVRCS 

       370        380        390        400        410        420 
HYPNDSRWYE LCDEYGLYVM DEANIETHGM TPMNRLTNDP TYLPLMSERV TRMVMRDRNH 

       430        440        450        460        470        480 
PSIIIWSLGN ESGYGSNHQA LYDWCKSFDS SRPVHYEGGD DASRGATDAT DIICPMYARV 

       490        500        510        520        530        540 
DSPSINAPYS LKTWMGVAGE NRPLILCEYA HDMGNSLGGF GKYWQAFREI DRLQGGFIWD 

       550        560        570        580        590        600 
WVDQGLLKDG NYAYGGDFGD KPNDRQFSLN GLVFPNRQAK PALREAKYWQ QYYQFELEKT 

       610        620        630        640        650        660 
PLGQVFAFTV TNEYLFRSTD NEKLCYQLIN GLEVLWENEL ILNMPAGGSM RIDLSELPID 

       670        680        690        700        710        720 
GTDNLFLNIQ VKTIEKCNLL ESDFEVAHQQ FVLQEKINFT DRIDSNEEIT LFEDEELLTV 

       730        740        750        760        770        780 
RSAKQKFIFN KSNGNLSRWL DEKGNEKLLH ELSEQFTRAP LDNDIGVSEV EHIDPNAWLE 

       790        800        810        820        830        840 
RWKGIGFYEL KTLLKTMIIQ ATENEVIISV QTDYEAKGKI AFSTIREYHI FRNGELLLKV 

       850        860        870        880        890        900 
DFKRNIEFPE PARIGLSLQL AEKAENVTYF GLGPDENYPD RRGASLFGQW NLRITDMTTP 

       910        920        930        940        950        960 
YIFPSENGLR METRELNYDR LKVRAMGQSF AFNLSPYSQN QLAKKGHWHL LEEEAGTWLN 

       970        980        990 
IDGFHMGVGG DDSWSPSVAQ EYLLTKGNYH YEVSFKLT 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and nucleotide sequence of the beta-galactosidase gene from Lactococcus lactis ssp. lactis ATCC7962."
Lee J.M., Park J.H., Lee W.K., Chang H.C., Kim J.H., Chung D.K., Lee H.J.
Biotechnol. Lett. 19:179-183(1997)
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 7962.
[2]"Cloning, DNA sequence, and regulation of expression of a gene encoding beta-galactosidase from Lactococcus lactis."
Griffin H.G., Maccormick C.A., Gasson M.J.
DNA Seq. 6:337-346(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 7962.
[3]"The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403."
Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J., Ehrlich S.D., Sorokin A.
Genome Res. 11:731-753(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: IL1403.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U60828 Genomic DNA. Translation: AAD11504.1.
X80037 Genomic DNA. Translation: CAA56341.1. Different initiation.
AE005176 Genomic DNA. Translation: AAK06078.1. Different initiation.
PIRD86872.
RefSeqNP_268137.2. NC_002662.1.

3D structure databases

ProteinModelPortalQ48727.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272623.L0025.

Protein family/group databases

CAZyGH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAK06078; AAK06078; L0025.
GeneID1115654.
KEGGlla:L0025.
PATRIC22296478. VBILacLac136773_2132.

Phylogenomic databases

eggNOGCOG3250.
HOGENOMHOG000252443.
KOK01190.
OMAHRCAWDQ.
OrthoDBEOG6XWV0T.
ProtClustDBPRK09525.

Enzyme and pathway databases

BioCycLLAC272623:GHSH-2108-MONOMER.
SABIO-RKQ48727.

Family and domain databases

Gene3D2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
InterProIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSPR00132. GLHYDRLASE2.
SMARTSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBGAL_LACLA
AccessionPrimary (citable) accession number: Q48727
Secondary accession number(s): P71448, Q9CE65
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: September 26, 2001
Last modified: November 13, 2013
This is version 100 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries