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Protein

Beta-galactosidase

Gene

lacZ

Organism
Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei431 – 4311Proton donorBy similarity
Active sitei508 – 5081NucleophileBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BioCyciLLAC272623:GHSH-2108-MONOMER.
SABIO-RKQ48727.

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase (EC:3.2.1.23)
Alternative name(s):
Lactase
Gene namesi
Name:lacZ
Ordered Locus Names:LL1980
ORF Names:L0025
OrganismiLactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)
Taxonomic identifieri272623 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus
ProteomesiUP000002196 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 998998Beta-galactosidasePRO_0000057669Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi272623.L0025.

Structurei

3D structure databases

ProteinModelPortaliQ48727.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 2 family.Curated

Phylogenomic databases

eggNOGiCOG3250.
HOGENOMiHOG000252443.
KOiK01190.
OMAiCEYAHDM.
OrthoDBiEOG6XWV0T.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q48727-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAMMTMIDVL ERKDWENPVV SNWNRLPMHT PMDLLEKQSL NGLWNFDHFS
60 70 80 90 100
RISDVPKNWL ELTESKTEII VPSNWQIEFK DKSDVPIYTN VTYPIPIQPP
110 120 130 140 150
YVPEANPVGA YSRYFDITKE WLESGHVHLT FEGVGSAFHF WLNGEYGGYS
160 170 180 190 200
EDSRLPAEFD ISNLAKEGQN CLKVLVFRWS KVTYFEDQDM WRMSGIFRSV
210 220 230 240 250
NLQWLPDNYL LDFSIKTDLD EDLDFANVKL QAYAKNIDDA CLEFKLYDDE
260 270 280 290 300
QLIGECHGFD AEIGVVNPKL WSDEIPYLYR LELTLMDRSG AVFHKETKKI
310 320 330 340 350
GIRKIAIEKG QLKINGKALL VRGVNKHEFT PEHGYVVSEE VMIKDIKLMK
360 370 380 390 400
EHNFNAVRCS HYPNDSRWYE LCDEYGLYVM DEANIETHGM TPMNRLTNDP
410 420 430 440 450
TYLPLMSERV TRMVMRDRNH PSIIIWSLGN ESGYGSNHQA LYDWCKSFDS
460 470 480 490 500
SRPVHYEGGD DASRGATDAT DIICPMYARV DSPSINAPYS LKTWMGVAGE
510 520 530 540 550
NRPLILCEYA HDMGNSLGGF GKYWQAFREI DRLQGGFIWD WVDQGLLKDG
560 570 580 590 600
NYAYGGDFGD KPNDRQFSLN GLVFPNRQAK PALREAKYWQ QYYQFELEKT
610 620 630 640 650
PLGQVFAFTV TNEYLFRSTD NEKLCYQLIN GLEVLWENEL ILNMPAGGSM
660 670 680 690 700
RIDLSELPID GTDNLFLNIQ VKTIEKCNLL ESDFEVAHQQ FVLQEKINFT
710 720 730 740 750
DRIDSNEEIT LFEDEELLTV RSAKQKFIFN KSNGNLSRWL DEKGNEKLLH
760 770 780 790 800
ELSEQFTRAP LDNDIGVSEV EHIDPNAWLE RWKGIGFYEL KTLLKTMIIQ
810 820 830 840 850
ATENEVIISV QTDYEAKGKI AFSTIREYHI FRNGELLLKV DFKRNIEFPE
860 870 880 890 900
PARIGLSLQL AEKAENVTYF GLGPDENYPD RRGASLFGQW NLRITDMTTP
910 920 930 940 950
YIFPSENGLR METRELNYDR LKVRAMGQSF AFNLSPYSQN QLAKKGHWHL
960 970 980 990
LEEEAGTWLN IDGFHMGVGG DDSWSPSVAQ EYLLTKGNYH YEVSFKLT
Length:998
Mass (Da):115,440
Last modified:September 26, 2001 - v3
Checksum:i8989FD4287ADDA89
GO

Sequence cautioni

The sequence AAK06078.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence CAA56341.1 differs from that shown. Reason: Erroneous initiation. Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti34 – 341L → F in strain: ATCC 7962.
Natural varianti182 – 1821V → G in strain: ATCC 7962.
Natural varianti237 – 2371I → M in strain: ATCC 7962.
Natural varianti629 – 6291I → T in strain: ATCC 7962.
Natural varianti677 – 6771C → Y in strain: ATCC 7962.
Natural varianti702 – 7021R → K in strain: ATCC 7962.
Natural varianti712 – 7121F → L in strain: ATCC 7962.
Natural varianti784 – 7852GI → AV in strain: ATCC 7962.
Natural varianti796 – 7961T → N in strain: ATCC 7962.
Natural varianti919 – 9191D → G in strain: ATCC 7962.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U60828 Genomic DNA. Translation: AAD11504.1.
X80037 Genomic DNA. Translation: CAA56341.1. Different initiation.
AE005176 Genomic DNA. Translation: AAK06078.1. Different initiation.
PIRiD86872.
RefSeqiNP_268137.2. NC_002662.1.
WP_010906246.1. NC_002662.1.

Genome annotation databases

EnsemblBacteriaiAAK06078; AAK06078; L0025.
GeneIDi1115654.
KEGGilla:L0025.
PATRICi22296478. VBILacLac136773_2132.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U60828 Genomic DNA. Translation: AAD11504.1.
X80037 Genomic DNA. Translation: CAA56341.1. Different initiation.
AE005176 Genomic DNA. Translation: AAK06078.1. Different initiation.
PIRiD86872.
RefSeqiNP_268137.2. NC_002662.1.
WP_010906246.1. NC_002662.1.

3D structure databases

ProteinModelPortaliQ48727.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272623.L0025.

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK06078; AAK06078; L0025.
GeneIDi1115654.
KEGGilla:L0025.
PATRICi22296478. VBILacLac136773_2132.

Phylogenomic databases

eggNOGiCOG3250.
HOGENOMiHOG000252443.
KOiK01190.
OMAiCEYAHDM.
OrthoDBiEOG6XWV0T.

Enzyme and pathway databases

BioCyciLLAC272623:GHSH-2108-MONOMER.
SABIO-RKQ48727.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and nucleotide sequence of the beta-galactosidase gene from Lactococcus lactis ssp. lactis ATCC7962."
    Lee J.M., Park J.H., Lee W.K., Chang H.C., Kim J.H., Chung D.K., Lee H.J.
    Biotechnol. Lett. 19:179-183(1997)
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 7962.
  2. "Cloning, DNA sequence, and regulation of expression of a gene encoding beta-galactosidase from Lactococcus lactis."
    Griffin H.G., Maccormick C.A., Gasson M.J.
    DNA Seq. 6:337-346(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 7962.
  3. "The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403."
    Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J., Ehrlich S.D., Sorokin A.
    Genome Res. 11:731-753(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: IL1403.

Entry informationi

Entry nameiBGAL_LACLA
AccessioniPrimary (citable) accession number: Q48727
Secondary accession number(s): P71448, Q9CE65
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: September 26, 2001
Last modified: May 27, 2015
This is version 104 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.