Q48727 (BGAL_LACLA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-galactosidase
EC=3.2.1.23

Alternative name(s):
Lactase

Gene names

Name:	lacZ
Ordered Locus Names:	LL1980
ORF Names:	L0025

Organism

Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)

Taxonomic identifier

272623 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Lactococcus

Protein attributes

Sequence length	998 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.
Sequence similarities	Belongs to the glycosyl hydrolase 2 family.
Sequence caution	The sequence AAK06078.1 differs from that shown. Reason: Erroneous initiation. The sequence CAA56341.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	beta-galactosidase complex Inferred from electronic annotation. Source: InterPro
Molecular function	beta-galactosidase activity Inferred from electronic annotation. Source: EC carbohydrate binding Inferred from electronic annotation. Source: InterPro cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 998	998	Beta-galactosidase		PRO_0000057669
Sites
	Active site	431	1	Proton donor By similarity
	Active site	508	1	Nucleophile By similarity
Natural variations
	Natural variant	34	1	L → F in strain: ATCC 7962.
	Natural variant	182	1	V → G in strain: ATCC 7962.
	Natural variant	237	1	I → M in strain: ATCC 7962.
	Natural variant	629	1	I → T in strain: ATCC 7962.
	Natural variant	677	1	C → Y in strain: ATCC 7962.
	Natural variant	702	1	R → K in strain: ATCC 7962.
	Natural variant	712	1	F → L in strain: ATCC 7962.
	Natural variant	784 – 785	2	GI → AV in strain: ATCC 7962.
	Natural variant	796	1	T → N in strain: ATCC 7962.
	Natural variant	919	1	D → G in strain: ATCC 7962.

Sequences

	Sequence		Length	Mass (Da)
	Q48727 [UniParc]. Last modified September 26, 2001. Version 3. Checksum: 8989FD4287ADDA89	FASTA	998	115,440
	10 20 30 40 50 60 MAMMTMIDVL ERKDWENPVV SNWNRLPMHT PMDLLEKQSL NGLWNFDHFS RISDVPKNWL 70 80 90 100 110 120 ELTESKTEII VPSNWQIEFK DKSDVPIYTN VTYPIPIQPP YVPEANPVGA YSRYFDITKE 130 140 150 160 170 180 WLESGHVHLT FEGVGSAFHF WLNGEYGGYS EDSRLPAEFD ISNLAKEGQN CLKVLVFRWS 190 200 210 220 230 240 KVTYFEDQDM WRMSGIFRSV NLQWLPDNYL LDFSIKTDLD EDLDFANVKL QAYAKNIDDA 250 260 270 280 290 300 CLEFKLYDDE QLIGECHGFD AEIGVVNPKL WSDEIPYLYR LELTLMDRSG AVFHKETKKI 310 320 330 340 350 360 GIRKIAIEKG QLKINGKALL VRGVNKHEFT PEHGYVVSEE VMIKDIKLMK EHNFNAVRCS 370 380 390 400 410 420 HYPNDSRWYE LCDEYGLYVM DEANIETHGM TPMNRLTNDP TYLPLMSERV TRMVMRDRNH 430 440 450 460 470 480 PSIIIWSLGN ESGYGSNHQA LYDWCKSFDS SRPVHYEGGD DASRGATDAT DIICPMYARV 490 500 510 520 530 540 DSPSINAPYS LKTWMGVAGE NRPLILCEYA HDMGNSLGGF GKYWQAFREI DRLQGGFIWD 550 560 570 580 590 600 WVDQGLLKDG NYAYGGDFGD KPNDRQFSLN GLVFPNRQAK PALREAKYWQ QYYQFELEKT 610 620 630 640 650 660 PLGQVFAFTV TNEYLFRSTD NEKLCYQLIN GLEVLWENEL ILNMPAGGSM RIDLSELPID 670 680 690 700 710 720 GTDNLFLNIQ VKTIEKCNLL ESDFEVAHQQ FVLQEKINFT DRIDSNEEIT LFEDEELLTV 730 740 750 760 770 780 RSAKQKFIFN KSNGNLSRWL DEKGNEKLLH ELSEQFTRAP LDNDIGVSEV EHIDPNAWLE 790 800 810 820 830 840 RWKGIGFYEL KTLLKTMIIQ ATENEVIISV QTDYEAKGKI AFSTIREYHI FRNGELLLKV 850 860 870 880 890 900 DFKRNIEFPE PARIGLSLQL AEKAENVTYF GLGPDENYPD RRGASLFGQW NLRITDMTTP 910 920 930 940 950 960 YIFPSENGLR METRELNYDR LKVRAMGQSF AFNLSPYSQN QLAKKGHWHL LEEEAGTWLN 970 980 990 IDGFHMGVGG DDSWSPSVAQ EYLLTKGNYH YEVSFKLT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and nucleotide sequence of the beta-galactosidase gene from Lactococcus lactis ssp. lactis ATCC7962." Lee J.M., Park J.H., Lee W.K., Chang H.C., Kim J.H., Chung D.K., Lee H.J. Biotechnol. Lett. 19:179-183(1997) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 7962.
[2]	"Cloning, DNA sequence, and regulation of expression of a gene encoding beta-galactosidase from Lactococcus lactis." Griffin H.G., Maccormick C.A., Gasson M.J. DNA Seq. 6:337-346(1996) [PubMed: 8988372] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 7962.
[3]	"The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403." Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J., Ehrlich S.D., Sorokin A. Genome Res. 11:731-753(2001) [PubMed: 11337471] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: IL1403.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U60828 Genomic DNA. Translation: AAD11504.1. X80037 Genomic DNA. Translation: CAA56341.1. Different initiation. AE005176 Genomic DNA. Translation: AAK06078.1. Different initiation.
PIR	D86872.
RefSeq	NP_268137.2. NC_002662.1.
3D structure databases
ProteinModelPortal	Q48727.
ModBase	Search...
Protein family/group databases
CAZy	GH2. Glycoside Hydrolase Family 2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1115654.
GenomeReviews	Gene locus LL1980 in contig AE005176_GR.
KEGG	lla:L0025.
NMPDR	fig|272623.1.peg.2033.
PATRIC	22296478. VBILacLac136773_2132.
Phylogenomic databases
HOGENOM	HBG670643.
OMA	IKITNTH.
ProtClustDB	PRK09525.
Enzyme and pathway databases
BioCyc	LLAC272623:L0025-MONOMER.
Family and domain databases
InterPro	IPR004199. B-gal_small/dom_5. IPR008979. Galactose-bd-like. IPR011013. Glyco_hydro-type_carb-bd. IPR014718. Glyco_hydro-type_carb-bd_sub. IPR006101. Glyco_hydro_2. IPR013812. Glyco_hydro_2/20_Ig-like. IPR023232. Glyco_hydro_2_AS. IPR023230. Glyco_hydro_2_CS. IPR006102. Glyco_hydro_2_Ig-like. IPR006104. Glyco_hydro_2_N. IPR006103. Glyco_hydro_2_TIM. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Gene3D	G3DSA:2.60.40.320. Glyco_hydro_2/20_Ig-like. 2 hits. G3DSA:2.70.98.10. Glyco_hydro_42_D5. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
KO	K01190.
Pfam	PF02929. Bgal_small_N. 1 hit. PF00703. Glyco_hydro_2. 1 hit. PF02836. Glyco_hydro_2_C. 1 hit. PF02837. Glyco_hydro_2_N. 1 hit. [Graphical view]
PRINTS	PR00132. GLHYDRLASE2.
SMART	SM01038. Bgal_small_N. 1 hit. [Graphical view]
SUPFAM	SSF49785. Gal_bind_like. 1 hit. SSF74650. Gal_mut_like. 1 hit. SSF49303. Glyco_hydro_2Ig. 2 hits. SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00719. GLYCOSYL_HYDROL_F2_1. 1 hit. PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BGAL_LACLA

Accession

Primary (citable) accession number: Q48727
Secondary accession number(s): P71448, Q9CE65

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	September 26, 2001
Last modified:	January 25, 2012
This is version 85 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program