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Q486U0

- SYI_COLP3

UniProt

Q486U0 - SYI_COLP3

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Protein
Isoleucine--tRNA ligase
Gene
ileS, CPS_1182
Organism
Colwellia psychrerythraea (strain 34H / ATCC BAA-681) (Vibrio psychroerythus)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei565 – 5651Aminoacyl-adenylate By similarity
Binding sitei609 – 6091ATP By similarity
Metal bindingi916 – 9161Zinc By similarity
Metal bindingi919 – 9191Zinc By similarity
Metal bindingi936 – 9361Zinc By similarity
Metal bindingi939 – 9391Zinc By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciCPSY167879:GI48-1182-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:CPS_1182
OrganismiColwellia psychrerythraea (strain 34H / ATCC BAA-681) (Vibrio psychroerythus)
Taxonomic identifieri167879 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesColwelliaceaeColwellia
ProteomesiUP000000547: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 953953Isoleucine--tRNA ligaseUniRule annotation
PRO_0000098376Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi167879.CPS_1182.

Structurei

3D structure databases

ProteinModelPortaliQ486U0.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionUniRule annotation
Add
BLAST
Motifi606 – 6105"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q486U0-1 [UniParc]FASTAAdd to Basket

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MSDYKQTLNL PATSFAMKGN MANREPNMLK YWAAKDLYGK IREAKKGKKS    50
FILHDGPPYA NGNIHLGHAV NKILKDIIVK SKNLSDFNSP FVPGWDCHGL 100
PIELMVEKKV GKPGHKISAS DFRQKCREYA AKQVNGQRED FKRLGIFADW 150
EKPYLTMDFG TEANIIRSLG KIAENGHLHQ GFKPVHWCTD CGSSLAEAEV 200
EYKDKQSPAI DVKFTISDES VADKFSHPEG HKGEGEIGAV IWTTTPWTLP 250
ANRAIAVNAE VEYTLVQCEQ AGEKQRLIIA SDLVTTCMDR FGFDKYHALG 300
FCKGSELELV QCQHPFYDFT VPVVLGEHVT TDSGTGCVHT APGHGVEDFV 350
VGKLYDLEVA NPVGANGVYL EDTPLLAGQH VFKANASVVE LLKEKGALVH 400
HHALDHSYPH CWRHKTPLIF RATPQWFISM DKKGLRQDSL NEIEKTQWIP 450
DWGQRRIESM VEGRPDWCIS RQRTWGVPMA LFIHQDSGAL HPRSIELIEE 500
VALLVEKSGI QAWFDLEAIE LIGDDAKEYI KVPDTLDVWF DSGTTHESVI 550
KARDEFDGIA DLYLEGSDQH RGWFMSSMIS SVAMNGAAPY KQVLTHGFVV 600
DAKGHKMSKS LGNVITPKEI TNNLGADILR LWTASVNYTQ EITAGDEIFK 650
RQADAYRRIR NTSRFLLSNL TGFEPANHMV AVEDMVALDR WVIDKAARLQ 700
EEIINAYDEY EFHVVVHKLM NFCTNELGGF YLDIIKDRQY TAKSDSNARR 750
SCQTAMYLIA EAMTAWMAPI LSFTAQEIWE ALPLPVSGER DEFVFTGVWF 800
DGLMKQESKQ DESTESSDEL GNEYWTELLT VRGEVNRALE QARKDKSVGK 850
ALEAQVTLFA TADLAAKLAK LGDELRFVLI TSKATIETVT SAPENALETE 900
VEGLWLTVAP AEGIKCERCW HVTTDIGESE KHPTLCGRCI TNIDGEGETR 950
QFA 953
Length:953
Mass (Da):106,844
Last modified:September 13, 2005 - v1
Checksum:iB6FDAC59AA903A26
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000083 Genomic DNA. Translation: AAZ24004.1.
RefSeqiYP_267925.1. NC_003910.7.

Genome annotation databases

EnsemblBacteriaiAAZ24004; AAZ24004; CPS_1182.
GeneIDi3518669.
KEGGicps:CPS_1182.
PATRICi21465613. VBIColPsy94388_1058.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000083 Genomic DNA. Translation: AAZ24004.1 .
RefSeqi YP_267925.1. NC_003910.7.

3D structure databases

ProteinModelPortali Q486U0.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 167879.CPS_1182.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAZ24004 ; AAZ24004 ; CPS_1182 .
GeneIDi 3518669.
KEGGi cps:CPS_1182.
PATRICi 21465613. VBIColPsy94388_1058.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci CPSY167879:GI48-1182-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 34H / ATCC BAA-681.

Entry informationi

Entry nameiSYI_COLP3
AccessioniPrimary (citable) accession number: Q486U0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: September 13, 2005
Last modified: May 14, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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