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Q486U0

- SYI_COLP3

UniProt

Q486U0 - SYI_COLP3

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Colwellia psychrerythraea (strain 34H / ATCC BAA-681) (Vibrio psychroerythus)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 69 (01 Oct 2014)
      Sequence version 1 (13 Sep 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei565 – 5651Aminoacyl-adenylateUniRule annotation
    Binding sitei609 – 6091ATPUniRule annotation
    Metal bindingi916 – 9161ZincUniRule annotation
    Metal bindingi919 – 9191ZincUniRule annotation
    Metal bindingi936 – 9361ZincUniRule annotation
    Metal bindingi939 – 9391ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciCPSY167879:GI48-1182-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:CPS_1182
    OrganismiColwellia psychrerythraea (strain 34H / ATCC BAA-681) (Vibrio psychroerythus)
    Taxonomic identifieri167879 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesColwelliaceaeColwellia
    ProteomesiUP000000547: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 953953Isoleucine--tRNA ligasePRO_0000098376Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi167879.CPS_1182.

    Structurei

    3D structure databases

    ProteinModelPortaliQ486U0.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi58 – 6811"HIGH" regionAdd
    BLAST
    Motifi606 – 6105"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q486U0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSDYKQTLNL PATSFAMKGN MANREPNMLK YWAAKDLYGK IREAKKGKKS    50
    FILHDGPPYA NGNIHLGHAV NKILKDIIVK SKNLSDFNSP FVPGWDCHGL 100
    PIELMVEKKV GKPGHKISAS DFRQKCREYA AKQVNGQRED FKRLGIFADW 150
    EKPYLTMDFG TEANIIRSLG KIAENGHLHQ GFKPVHWCTD CGSSLAEAEV 200
    EYKDKQSPAI DVKFTISDES VADKFSHPEG HKGEGEIGAV IWTTTPWTLP 250
    ANRAIAVNAE VEYTLVQCEQ AGEKQRLIIA SDLVTTCMDR FGFDKYHALG 300
    FCKGSELELV QCQHPFYDFT VPVVLGEHVT TDSGTGCVHT APGHGVEDFV 350
    VGKLYDLEVA NPVGANGVYL EDTPLLAGQH VFKANASVVE LLKEKGALVH 400
    HHALDHSYPH CWRHKTPLIF RATPQWFISM DKKGLRQDSL NEIEKTQWIP 450
    DWGQRRIESM VEGRPDWCIS RQRTWGVPMA LFIHQDSGAL HPRSIELIEE 500
    VALLVEKSGI QAWFDLEAIE LIGDDAKEYI KVPDTLDVWF DSGTTHESVI 550
    KARDEFDGIA DLYLEGSDQH RGWFMSSMIS SVAMNGAAPY KQVLTHGFVV 600
    DAKGHKMSKS LGNVITPKEI TNNLGADILR LWTASVNYTQ EITAGDEIFK 650
    RQADAYRRIR NTSRFLLSNL TGFEPANHMV AVEDMVALDR WVIDKAARLQ 700
    EEIINAYDEY EFHVVVHKLM NFCTNELGGF YLDIIKDRQY TAKSDSNARR 750
    SCQTAMYLIA EAMTAWMAPI LSFTAQEIWE ALPLPVSGER DEFVFTGVWF 800
    DGLMKQESKQ DESTESSDEL GNEYWTELLT VRGEVNRALE QARKDKSVGK 850
    ALEAQVTLFA TADLAAKLAK LGDELRFVLI TSKATIETVT SAPENALETE 900
    VEGLWLTVAP AEGIKCERCW HVTTDIGESE KHPTLCGRCI TNIDGEGETR 950
    QFA 953
    Length:953
    Mass (Da):106,844
    Last modified:September 13, 2005 - v1
    Checksum:iB6FDAC59AA903A26
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000083 Genomic DNA. Translation: AAZ24004.1.
    RefSeqiYP_267925.1. NC_003910.7.

    Genome annotation databases

    EnsemblBacteriaiAAZ24004; AAZ24004; CPS_1182.
    GeneIDi3518669.
    KEGGicps:CPS_1182.
    PATRICi21465613. VBIColPsy94388_1058.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000083 Genomic DNA. Translation: AAZ24004.1 .
    RefSeqi YP_267925.1. NC_003910.7.

    3D structure databases

    ProteinModelPortali Q486U0.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 167879.CPS_1182.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAZ24004 ; AAZ24004 ; CPS_1182 .
    GeneIDi 3518669.
    KEGGi cps:CPS_1182.
    PATRICi 21465613. VBIColPsy94388_1058.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci CPSY167879:GI48-1182-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 34H / ATCC BAA-681.

    Entry informationi

    Entry nameiSYI_COLP3
    AccessioniPrimary (citable) accession number: Q486U0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: September 13, 2005
    Last modified: October 1, 2014
    This is version 69 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3