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Q486U0

- SYI_COLP3

UniProt

Q486U0 - SYI_COLP3

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Colwellia psychrerythraea (strain 34H / ATCC BAA-681) (Vibrio psychroerythus)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei565 – 5651Aminoacyl-adenylateUniRule annotation
Binding sitei609 – 6091ATPUniRule annotation
Metal bindingi916 – 9161ZincUniRule annotation
Metal bindingi919 – 9191ZincUniRule annotation
Metal bindingi936 – 9361ZincUniRule annotation
Metal bindingi939 – 9391ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciCPSY167879:GI48-1182-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:CPS_1182
OrganismiColwellia psychrerythraea (strain 34H / ATCC BAA-681) (Vibrio psychroerythus)
Taxonomic identifieri167879 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesColwelliaceaeColwellia
ProteomesiUP000000547: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 953953Isoleucine--tRNA ligasePRO_0000098376Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi167879.CPS_1182.

Structurei

3D structure databases

ProteinModelPortaliQ486U0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionAdd
BLAST
Motifi606 – 6105"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q486U0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSDYKQTLNL PATSFAMKGN MANREPNMLK YWAAKDLYGK IREAKKGKKS
60 70 80 90 100
FILHDGPPYA NGNIHLGHAV NKILKDIIVK SKNLSDFNSP FVPGWDCHGL
110 120 130 140 150
PIELMVEKKV GKPGHKISAS DFRQKCREYA AKQVNGQRED FKRLGIFADW
160 170 180 190 200
EKPYLTMDFG TEANIIRSLG KIAENGHLHQ GFKPVHWCTD CGSSLAEAEV
210 220 230 240 250
EYKDKQSPAI DVKFTISDES VADKFSHPEG HKGEGEIGAV IWTTTPWTLP
260 270 280 290 300
ANRAIAVNAE VEYTLVQCEQ AGEKQRLIIA SDLVTTCMDR FGFDKYHALG
310 320 330 340 350
FCKGSELELV QCQHPFYDFT VPVVLGEHVT TDSGTGCVHT APGHGVEDFV
360 370 380 390 400
VGKLYDLEVA NPVGANGVYL EDTPLLAGQH VFKANASVVE LLKEKGALVH
410 420 430 440 450
HHALDHSYPH CWRHKTPLIF RATPQWFISM DKKGLRQDSL NEIEKTQWIP
460 470 480 490 500
DWGQRRIESM VEGRPDWCIS RQRTWGVPMA LFIHQDSGAL HPRSIELIEE
510 520 530 540 550
VALLVEKSGI QAWFDLEAIE LIGDDAKEYI KVPDTLDVWF DSGTTHESVI
560 570 580 590 600
KARDEFDGIA DLYLEGSDQH RGWFMSSMIS SVAMNGAAPY KQVLTHGFVV
610 620 630 640 650
DAKGHKMSKS LGNVITPKEI TNNLGADILR LWTASVNYTQ EITAGDEIFK
660 670 680 690 700
RQADAYRRIR NTSRFLLSNL TGFEPANHMV AVEDMVALDR WVIDKAARLQ
710 720 730 740 750
EEIINAYDEY EFHVVVHKLM NFCTNELGGF YLDIIKDRQY TAKSDSNARR
760 770 780 790 800
SCQTAMYLIA EAMTAWMAPI LSFTAQEIWE ALPLPVSGER DEFVFTGVWF
810 820 830 840 850
DGLMKQESKQ DESTESSDEL GNEYWTELLT VRGEVNRALE QARKDKSVGK
860 870 880 890 900
ALEAQVTLFA TADLAAKLAK LGDELRFVLI TSKATIETVT SAPENALETE
910 920 930 940 950
VEGLWLTVAP AEGIKCERCW HVTTDIGESE KHPTLCGRCI TNIDGEGETR

QFA
Length:953
Mass (Da):106,844
Last modified:September 13, 2005 - v1
Checksum:iB6FDAC59AA903A26
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000083 Genomic DNA. Translation: AAZ24004.1.
RefSeqiYP_267925.1. NC_003910.7.

Genome annotation databases

EnsemblBacteriaiAAZ24004; AAZ24004; CPS_1182.
GeneIDi3518669.
KEGGicps:CPS_1182.
PATRICi21465613. VBIColPsy94388_1058.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000083 Genomic DNA. Translation: AAZ24004.1 .
RefSeqi YP_267925.1. NC_003910.7.

3D structure databases

ProteinModelPortali Q486U0.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 167879.CPS_1182.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAZ24004 ; AAZ24004 ; CPS_1182 .
GeneIDi 3518669.
KEGGi cps:CPS_1182.
PATRICi 21465613. VBIColPsy94388_1058.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci CPSY167879:GI48-1182-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 34H / ATCC BAA-681.

Entry informationi

Entry nameiSYI_COLP3
AccessioniPrimary (citable) accession number: Q486U0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: September 13, 2005
Last modified: November 26, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3