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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Colwellia psychrerythraea (strain 34H / ATCC BAA-681) (Vibrio psychroerythus)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei565Aminoacyl-adenylateUniRule annotation1
Binding sitei609ATPUniRule annotation1
Metal bindingi916ZincUniRule annotation1
Metal bindingi919ZincUniRule annotation1
Metal bindingi936ZincUniRule annotation1
Metal bindingi939ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciCPSY167879:G1G4N-1076-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:CPS_1182
OrganismiColwellia psychrerythraea (strain 34H / ATCC BAA-681) (Vibrio psychroerythus)
Taxonomic identifieri167879 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesColwelliaceaeColwellia
Proteomesi
  • UP000000547 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000983761 – 953Isoleucine--tRNA ligaseAdd BLAST953

Proteomic databases

PRIDEiQ486U0

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi167879.CPS_1182

Structurei

3D structure databases

ProteinModelPortaliQ486U0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi58 – 68"HIGH" regionAdd BLAST11
Motifi606 – 610"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C07 Bacteria
COG0060 LUCA
HOGENOMiHOG000246402
KOiK01870
OMAiHLGTAWN
OrthoDBiPOG091H028I

Family and domain databases

CDDicd07960 Anticodon_Ia_Ile_BEm, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02002 Ile_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033708 Anticodon_Ile_BEm
IPR002301 Ile-tRNA-ligase
IPR023585 Ile-tRNA-ligase_type1
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
IPR010663 Znf_FPG/IleRS
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PF06827 zf-FPG_IleRS, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Q486U0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDYKQTLNL PATSFAMKGN MANREPNMLK YWAAKDLYGK IREAKKGKKS
60 70 80 90 100
FILHDGPPYA NGNIHLGHAV NKILKDIIVK SKNLSDFNSP FVPGWDCHGL
110 120 130 140 150
PIELMVEKKV GKPGHKISAS DFRQKCREYA AKQVNGQRED FKRLGIFADW
160 170 180 190 200
EKPYLTMDFG TEANIIRSLG KIAENGHLHQ GFKPVHWCTD CGSSLAEAEV
210 220 230 240 250
EYKDKQSPAI DVKFTISDES VADKFSHPEG HKGEGEIGAV IWTTTPWTLP
260 270 280 290 300
ANRAIAVNAE VEYTLVQCEQ AGEKQRLIIA SDLVTTCMDR FGFDKYHALG
310 320 330 340 350
FCKGSELELV QCQHPFYDFT VPVVLGEHVT TDSGTGCVHT APGHGVEDFV
360 370 380 390 400
VGKLYDLEVA NPVGANGVYL EDTPLLAGQH VFKANASVVE LLKEKGALVH
410 420 430 440 450
HHALDHSYPH CWRHKTPLIF RATPQWFISM DKKGLRQDSL NEIEKTQWIP
460 470 480 490 500
DWGQRRIESM VEGRPDWCIS RQRTWGVPMA LFIHQDSGAL HPRSIELIEE
510 520 530 540 550
VALLVEKSGI QAWFDLEAIE LIGDDAKEYI KVPDTLDVWF DSGTTHESVI
560 570 580 590 600
KARDEFDGIA DLYLEGSDQH RGWFMSSMIS SVAMNGAAPY KQVLTHGFVV
610 620 630 640 650
DAKGHKMSKS LGNVITPKEI TNNLGADILR LWTASVNYTQ EITAGDEIFK
660 670 680 690 700
RQADAYRRIR NTSRFLLSNL TGFEPANHMV AVEDMVALDR WVIDKAARLQ
710 720 730 740 750
EEIINAYDEY EFHVVVHKLM NFCTNELGGF YLDIIKDRQY TAKSDSNARR
760 770 780 790 800
SCQTAMYLIA EAMTAWMAPI LSFTAQEIWE ALPLPVSGER DEFVFTGVWF
810 820 830 840 850
DGLMKQESKQ DESTESSDEL GNEYWTELLT VRGEVNRALE QARKDKSVGK
860 870 880 890 900
ALEAQVTLFA TADLAAKLAK LGDELRFVLI TSKATIETVT SAPENALETE
910 920 930 940 950
VEGLWLTVAP AEGIKCERCW HVTTDIGESE KHPTLCGRCI TNIDGEGETR

QFA
Length:953
Mass (Da):106,844
Last modified:September 13, 2005 - v1
Checksum:iB6FDAC59AA903A26
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000083 Genomic DNA Translation: AAZ24004.1
RefSeqiWP_011042019.1, NC_003910.7

Genome annotation databases

EnsemblBacteriaiAAZ24004; AAZ24004; CPS_1182
KEGGicps:CPS_1182

Similar proteinsi

Entry informationi

Entry nameiSYI_COLP3
AccessioniPrimary (citable) accession number: Q486U0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: September 13, 2005
Last modified: May 23, 2018
This is version 94 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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