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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Colwellia psychrerythraea (strain 34H / ATCC BAA-681) (Vibrio psychroerythus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei565 – 5651Aminoacyl-adenylateUniRule annotation
Binding sitei609 – 6091ATPUniRule annotation
Metal bindingi916 – 9161ZincUniRule annotation
Metal bindingi919 – 9191ZincUniRule annotation
Metal bindingi936 – 9361ZincUniRule annotation
Metal bindingi939 – 9391ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciCPSY167879:GI48-1182-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:CPS_1182
OrganismiColwellia psychrerythraea (strain 34H / ATCC BAA-681) (Vibrio psychroerythus)
Taxonomic identifieri167879 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesColwelliaceaeColwellia
ProteomesiUP000000547: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 953953Isoleucine--tRNA ligasePRO_0000098376Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi167879.CPS_1182.

Structurei

3D structure databases

ProteinModelPortaliQ486U0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionAdd
BLAST
Motifi606 – 6105"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q486U0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSDYKQTLNL PATSFAMKGN MANREPNMLK YWAAKDLYGK IREAKKGKKS
60 70 80 90 100
FILHDGPPYA NGNIHLGHAV NKILKDIIVK SKNLSDFNSP FVPGWDCHGL
110 120 130 140 150
PIELMVEKKV GKPGHKISAS DFRQKCREYA AKQVNGQRED FKRLGIFADW
160 170 180 190 200
EKPYLTMDFG TEANIIRSLG KIAENGHLHQ GFKPVHWCTD CGSSLAEAEV
210 220 230 240 250
EYKDKQSPAI DVKFTISDES VADKFSHPEG HKGEGEIGAV IWTTTPWTLP
260 270 280 290 300
ANRAIAVNAE VEYTLVQCEQ AGEKQRLIIA SDLVTTCMDR FGFDKYHALG
310 320 330 340 350
FCKGSELELV QCQHPFYDFT VPVVLGEHVT TDSGTGCVHT APGHGVEDFV
360 370 380 390 400
VGKLYDLEVA NPVGANGVYL EDTPLLAGQH VFKANASVVE LLKEKGALVH
410 420 430 440 450
HHALDHSYPH CWRHKTPLIF RATPQWFISM DKKGLRQDSL NEIEKTQWIP
460 470 480 490 500
DWGQRRIESM VEGRPDWCIS RQRTWGVPMA LFIHQDSGAL HPRSIELIEE
510 520 530 540 550
VALLVEKSGI QAWFDLEAIE LIGDDAKEYI KVPDTLDVWF DSGTTHESVI
560 570 580 590 600
KARDEFDGIA DLYLEGSDQH RGWFMSSMIS SVAMNGAAPY KQVLTHGFVV
610 620 630 640 650
DAKGHKMSKS LGNVITPKEI TNNLGADILR LWTASVNYTQ EITAGDEIFK
660 670 680 690 700
RQADAYRRIR NTSRFLLSNL TGFEPANHMV AVEDMVALDR WVIDKAARLQ
710 720 730 740 750
EEIINAYDEY EFHVVVHKLM NFCTNELGGF YLDIIKDRQY TAKSDSNARR
760 770 780 790 800
SCQTAMYLIA EAMTAWMAPI LSFTAQEIWE ALPLPVSGER DEFVFTGVWF
810 820 830 840 850
DGLMKQESKQ DESTESSDEL GNEYWTELLT VRGEVNRALE QARKDKSVGK
860 870 880 890 900
ALEAQVTLFA TADLAAKLAK LGDELRFVLI TSKATIETVT SAPENALETE
910 920 930 940 950
VEGLWLTVAP AEGIKCERCW HVTTDIGESE KHPTLCGRCI TNIDGEGETR

QFA
Length:953
Mass (Da):106,844
Last modified:September 13, 2005 - v1
Checksum:iB6FDAC59AA903A26
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000083 Genomic DNA. Translation: AAZ24004.1.
RefSeqiYP_267925.1. NC_003910.7.

Genome annotation databases

EnsemblBacteriaiAAZ24004; AAZ24004; CPS_1182.
GeneIDi3518669.
KEGGicps:CPS_1182.
PATRICi21465613. VBIColPsy94388_1058.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000083 Genomic DNA. Translation: AAZ24004.1.
RefSeqiYP_267925.1. NC_003910.7.

3D structure databases

ProteinModelPortaliQ486U0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi167879.CPS_1182.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAZ24004; AAZ24004; CPS_1182.
GeneIDi3518669.
KEGGicps:CPS_1182.
PATRICi21465613. VBIColPsy94388_1058.

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Enzyme and pathway databases

BioCyciCPSY167879:GI48-1182-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 34H / ATCC BAA-681.

Entry informationi

Entry nameiSYI_COLP3
AccessioniPrimary (citable) accession number: Q486U0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: September 13, 2005
Last modified: January 7, 2015
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.