Q486R1 (ISPH_COLP3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-hydroxy-3-methylbut-2-enyl diphosphate reductase
EC=1.17.1.2

Gene names

Name:	ispH
Ordered Locus Names:	CPS_1211

Organism

Colwellia psychrerythraea (strain 34H / ATCC BAA-681) (Vibrio psychroerythus) [Complete proteome] [HAMAP]

Taxonomic identifier

167879 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Colwelliaceae › Colwellia › Colwellia psychrerythraea

Protein attributes

Sequence length	309 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) By similarity. HAMAP-Rule MF_00191
Catalytic activity	Isopentenyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP-Rule MF_00191 Dimethylallyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP-Rule MF_00191
Cofactor	Binds 1 3Fe-4S cluster per subunit By similarity.
Pathway	Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate: step 1/1. HAMAP-Rule MF_00191 Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 6/6. HAMAP-Rule MF_00191
Sequence similarities	Belongs to the IspH family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	3Fe-4S Iron Iron-sulfur Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	dimethylallyl diphosphate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway isopentenyl diphosphate biosynthetic process, mevalonate-independent pathway Inferred from electronic annotation. Source: HAMAP terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: HAMAP 4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 309	309	4-hydroxy-3-methylbut-2-enyl diphosphate reductase HAMAP-Rule MF_00191		PRO_1000021108
Regions
	Region	225 – 227	3	Substrate binding By similarity
Sites
	Metal binding	12	1	Iron-sulfur (3Fe-4S) By similarity
	Metal binding	96	1	Iron-sulfur (3Fe-4S) By similarity
	Metal binding	197	1	Iron-sulfur (3Fe-4S) By similarity
	Binding site	41	1	Substrate By similarity
	Binding site	74	1	Substrate By similarity
	Binding site	124	1	Substrate By similarity
	Binding site	167	1	Substrate By similarity
	Binding site	269	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q486R1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 3FEA7D6D3D3A1A2B	FASTA	309	33,589
	10 20 30 40 50 60 MEIILANPRG FCAGVDRAIS IVERALDLFG APIYVRHEVV HNKFVVDGLK DRGAIFVDEL 70 80 90 100 110 120 DEVPDDNTVI FSAHGVSKAV RQEAKTRALK VFDATCPLVT KVHMEVSRVS RKDIECILIG 130 140 150 160 170 180 HAGHPEVEGT MGQYSSDSAG IYLVESPEDV ASLVVKNPEK LYFCSQTTLS VDDTMDVIAA 190 200 210 220 230 240 LQAKFPLIEG PRKDDICYAT QNRQDAVREI ADKVDLMLVV GAKNSSNSNR LKELASKMGV 250 260 270 280 290 300 TSYLIDTAEN IETDWLTGVN KVGVTAGASA PAVLIKQVVE ALKSYGGHQV IEHPGKEESI VFAVPAELR

References

[1]

"The psychrophilic lifestyle as revealed by the genome sequence of Colwellia psychrerythraea 34H through genomic and proteomic analyses." Methe B.A., Nelson K.E., Deming J.W., Momen B., Melamud E., Zhang X., Moult J., Madupu R., Nelson W.C., Dodson R.J., Brinkac L.M., Daugherty S.C., Durkin A.S., DeBoy R.T., Kolonay J.F., Sullivan S.A., Zhou L., Davidsen T.M. , Wu M., Huston A.L., Lewis M., Weaver B., Weidman J.F., Khouri H., Utterback T.R., Feldblyum T.V., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 102:10913-10918(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 34H / ATCC BAA-681.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000083 Genomic DNA. Translation: AAZ24157.1.
RefSeq	YP_267954.1. NC_003910.7.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	167879.CPS_1211.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAZ24157; AAZ24157; CPS_1211.
GeneID	3519583.
KEGG	cps:CPS_1211.
PATRIC	21465667. VBIColPsy94388_1085.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0761.
HOGENOM	HOG000220192.
KO	K03527.
OMA	LVTKVHQ.
ProtClustDB	PRK01045.
Enzyme and pathway databases
BioCyc	CPSY167879:GI48-1292-MONOMER.
UniPathway	UPA00056; UER00097. UPA00059; UER00105.
Family and domain databases
HAMAP	MF_00191. IspH.
InterPro	IPR003451. LytB. [Graphical view]
Pfam	PF02401. LYTB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00216. ispH_lytB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPH_COLP3

Accession

Primary (citable) accession number: Q486R1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 13, 2005
Last modified:	May 1, 2013
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program