Reviewed, UniProtKB/Swiss-Prot Q486J8 (GCST_COLP3)

Names and origin

Protein names

Recommended name:
    Aminomethyltransferase
    EC=2.1.2.10
Alternative name(s):
    Glycine cleavage system T protein

Gene names

Name:	gcvT
Ordered Locus Names:	CPS_1274

Organism

Colwellia psychrerythraea (strain 34H / ATCC BAA-681) (Vibrio psychroerythus) [Complete proteome] [HAMAP]

Taxonomic identifier

167879 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Colwelliaceae › Colwellia

Protein attributes

Sequence length	362 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	The glycine cleavage system catalyzes the degradation of glycine By similarity.
Catalytic activity	[Protein]-S(8)-aminomethyldihydrolipoyllysine + tetrahydrofolate = [protein]-dihydrolipoyllysine + 5,10-methylenetetrahydrofolate + NH3. HAMAP MF_00259
Subunit structure	The glycine cleavage system is composed of four proteins: P, T, L and H By similarity.
Sequence similarities	Belongs to the gcvT family.

Ontologies

Keywords
Molecular function	Aminotransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycine decarboxylation via glycine cleavage system Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	aminomethyltransferase activity Inferred from electronic annotation. Source: HAMAP transaminase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 362	362	Aminomethyltransferase HAMAP MF_00259		PRO_1000047659

Sequences

	Sequence		Length	Mass (Da)
	Q486J8-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: D74FA34F38C42F5A	FASTA	362	39,590
	10 20 30 40 50 60 MTNKTVLHAK HLASGAKMVD FFGWDMPINY GSQIEEHHAV RTDAGMFDVS HMTIVDVQGA 70 80 90 100 110 120 DAKAFLRRLV INDVAKLATP GKALYTGMLN EEGGVIDDLI IYFFSDTDYR LVVNSATRVK 130 140 150 160 170 180 DLAWMTKQST GFDITITERP EFGMLAVQGP EAKAKVAKLL TAEQIEAVEG MKPFFGVQVG 190 200 210 220 230 240 DLFIATTGYT GEDGYEIIVP NNSAEDFWQK LLDEGVVPCG LGARDTLRLE AGMNLYGLDM 250 260 270 280 290 300 DETVSPLAAN MAWTISWEPT DRDFIGRDVL TAQKAAGDQP KLVGLVLEAK GVLRSHQVVV 310 320 330 340 350 360 TEFGNGEITS GTFSPTLGHS VALARVPRSV KVGDTIEVEM RKKLIKVQVT KPSFVRNGKK VF

References

[1]

"The psychrophilic lifestyle as revealed by the genome sequence of Colwellia psychrerythraea 34H through genomic and proteomic analyses." Methe B.A., Nelson K.E., Deming J.W., Momen B., Melamud E., Zhang X., Moult J., Madupu R., Nelson W.C., Dodson R.J., Brinkac L.M., Daugherty S.C., Durkin A.S., DeBoy R.T., Kolonay J.F., Sullivan S.A., Zhou L., Davidsen T.M. , Wu M., Huston A.L., Lewis M., Weaver B., Weidman J.F., Khouri H., Utterback T.R., Feldblyum T.V., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 102:10913-10918(2005) [PubMed: 16043709] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000083 Genomic DNA. Translation: AAZ28390.1.
RefSeq	YP_268017.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3522650.
GenomeReviews	Gene locus CPS_1274 in contig CP000083_GR.
KEGG	cps:CPS_1274.
NMPDR	fig|167879.3.peg.1235.
TIGR	CPS_1274.
Phylogenomic databases
HOGENOM	Q486J8.
OMA	Q486J8. VEIRGKW.
Enzyme and pathway databases
BioCyc	CPSY167879:CPS_1274-MON.
Family and domain databases
HAMAP	MF_00259. [Tree]
InterPro	IPR013977. GCV_T_C. IPR006222. GCV_T_N. IPR006223. GcvT. [Graphical view]
Pfam	PF01571. GCV_T. 1 hit. PF08669. GCV_T_C. 1 hit. [Graphical view]
PIRSF	PIRSF006487. GcvT. 1 hit.
TIGRFAMs	TIGR00528. gcvT. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GCST_COLP3

Accession

Primary (citable) accession number: Q486J8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 13, 2005
Last modified:	June 16, 2009
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)