Q48662 (MLES_LACLA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Malolactic enzyme
EC=1.-.-.-

Gene names

Name:	mleS
Ordered Locus Names:	LL0900
ORF Names:	L121483

Organism

Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)

Taxonomic identifier

272623 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Lactococcus

Protein attributes

Sequence length	540 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Key enzyme in the degradation of L-malic acid by lactic acid bacteria.
Catalytic activity	(S)-malate + NAD+ = lactate + CO2 + NADH.
Cofactor	Divalent metal cations. Prefers magnesium or manganese By similarity.
Sequence similarities	Belongs to the malic enzymes family.
Sequence caution	The sequence CAA50716.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Ligand	Metal-binding NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	malate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro malate dehydrogenase (oxaloacetate-decarboxylating) activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 540	540	Malolactic enzyme		PRO_0000160249
Sites
	Active site	90	1	Proton donor By similarity
	Active site	163	1	Proton acceptor By similarity
	Metal binding	234	1	Divalent metal cation By similarity
	Metal binding	235	1	Divalent metal cation By similarity
	Metal binding	258	1	Divalent metal cation By similarity
	Binding site	258	1	NAD By similarity
	Binding site	403	1	NAD By similarity
	Site	258	1	Important for activity By similarity
Experimental info
	Sequence conflict	44	1	E → V Ref.1
	Sequence conflict	128	1	N → S Ref.1
	Sequence conflict	452	1	Y → H Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q48662 [UniParc]. Last modified April 27, 2001. Version 2. Checksum: D7ED86C76BA676D8	FASTA	540	59,517
	10 20 30 40 50 60 MRAHEILNNP FLNKGTAFTM KERQELGLIG LLPPTVQTIE EQAEQTYEQY LTKPSDLEKR 70 80 90 100 110 120 HFLMEIFNTN RTLFYYLFNK HIVEFNPVVY DPTIADTIEN YSHLFVDPQY AAYLDINHPE 130 140 150 160 170 180 NITETLKNAA GDREIRLIVV TDAEGILGIG DWGTQGVDIS VGKLMIYTAA AGIDPASVLP 190 200 210 220 230 240 VVIDAGTNRK ELLEDHLYLG NHQERIYGDQ YYSFVDQFVE TAESIFPKLY LHWEDFGRSN 250 260 270 280 290 300 AATILNNYKT KIPTFNDDIQ GTGIVVLGGI FGSLDITGEK LTDQVYLCYG GGSAGAGIAG 310 320 330 340 350 360 RVHAEMVSEG LSEEEAYKHF FMIDQQGLLF DDMEDLTPAQ KPFAKKRADY KDAGDMTDLL 370 380 390 400 410 420 NVVKTVKPTI LVGTSTNPGA FTKEVVEAMC ANTERPVIFP ISNPTKKMET TAEQVIEWSD 430 440 450 460 470 480 GKAFVATGVP SGTISYKGVD YQIGQANNSL IYPGLGLGML ASEAKLLTDE MIGAAAHSLS 490 500 510 520 530 540 GLVDPGKPGA PVLPPFEFVA DVSIKVAEAV AKKAQEQGLT ESKETDMAKA VRDLKWYPEY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, sequence and expression of the gene encoding the malolactic enzyme from Lactococcus lactis." Ansanay V., Dequin S., Blondin B., Barre P. FEBS Lett. 332:74-80(1993) [PubMed: 8405453] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: IL1441.
[2]	"Cloning and sequence analysis of the gene encoding Lactococcus lactis malolactic enzyme: relationships with malic enzymes." Denayrolles M., Aigle M., Lonvaud-Funel A. FEMS Microbiol. Lett. 116:79-86(1994) [PubMed: 8132158] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: IL1441.
[3]	"The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403." Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J., Ehrlich S.D., Sorokin A. Genome Res. 11:731-753(2001) [PubMed: 11337471] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: IL1403.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X75982 Genomic DNA. Translation: CAA53589.1. X71897 Genomic DNA. Translation: CAA50716.1. Different initiation. AE005176 Genomic DNA. Translation: AAK04998.1.
PIR	D86737. S38728.
RefSeq	NP_267056.1. NC_002662.1.
3D structure databases
ProteinModelPortal	Q48662.
ModBase	Search...
PTM databases
PhosSite	Q48662.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1114530.
GenomeReviews	Gene locus LL0900 in contig AE005176_GR.
KEGG	lla:L121483.
NMPDR	fig|272623.1.peg.922.
PATRIC	22294116. VBILacLac136773_0965.
Phylogenomic databases
HOGENOM	HBG289821.
OMA	QDNNETL.
ProtClustDB	PRK13529.
Enzyme and pathway databases
BioCyc	LLAC272623:L121483-MONOMER.
Family and domain databases
InterPro	IPR015884. Malic_enzyme_CS. IPR012301. Malic_N. IPR012302. Malic_NAD-bd. IPR001891. Malic_OxRdtase. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.10380. G3DSA:3.40.50.10380. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00027.
Pfam	PF00390. malic. 1 hit. PF03949. Malic_M. 1 hit. [Graphical view]
PIRSF	PIRSF000106. ME. 1 hit.
PRINTS	PR00072. MALOXRDTASE.
SMART	SM00919. Malic_M. 1 hit. [Graphical view]
PROSITE	PS00331. MALIC_ENZYMES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MLES_LACLA

Accession

Primary (citable) accession number: Q48662
Secondary accession number(s): Q48645

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	April 27, 2001
Last modified:	January 25, 2012
This is version 87 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program