Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q48661

- DEF_LACLA

UniProt

Q48661 - DEF_LACLA

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Peptide deformylase

Gene

def

Organism
Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions (By similarity).By similarity

Catalytic activityi

Formyl-L-methionyl peptide + H2O = formate + methionyl peptide.

Cofactori

Binds 1 Fe2+ ion.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi123 – 1231IronBy similarity
Metal bindingi166 – 1661IronBy similarity
Active sitei167 – 1671By similarity
Metal bindingi170 – 1701IronBy similarity

GO - Molecular functioni

  1. iron ion binding Source: InterPro
  2. peptide deformylase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. translation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BioCyciLLAC272623:GHSH-617-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Peptide deformylase (EC:3.5.1.88)
Short name:
PDF
Alternative name(s):
Polypeptide deformylase
Gene namesi
Name:def
Ordered Locus Names:LL0560
ORF Names:L154885
OrganismiLactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)
Taxonomic identifieri272623 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus
ProteomesiUP000002196: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 196196Peptide deformylasePRO_0000082792Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi272623.L154885.

Structurei

3D structure databases

ProteinModelPortaliQ48661.
SMRiQ48661. Positions 1-195.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the polypeptide deformylase family.Curated

Phylogenomic databases

eggNOGiCOG0242.
HOGENOMiHOG000243507.
KOiK01462.
OMAiSQDPKIA.
OrthoDBiEOG6PZXGQ.

Family and domain databases

Gene3Di3.90.45.10. 1 hit.
HAMAPiMF_00163. Pep_deformylase.
InterProiIPR000181. Fmet_deformylase.
IPR023635. Peptide_deformylase.
[Graphical view]
PANTHERiPTHR10458. PTHR10458. 1 hit.
PfamiPF01327. Pep_deformylase. 1 hit.
[Graphical view]
PIRSFiPIRSF004749. Pep_def. 1 hit.
PRINTSiPR01576. PDEFORMYLASE.
SUPFAMiSSF56420. SSF56420. 1 hit.
TIGRFAMsiTIGR00079. pept_deformyl. 1 hit.

Sequencei

Sequence statusi: Complete.

Q48661-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MISMDDIIRE GYPTLREVAN DVTLPLSDED IILGEKMLQF LHNSQDPVMA
60 70 80 90 100
EKMGLRGGVG LAANQLGLLK KVIAVLIPNE PEVDEDGNEI PPKEAYKMRE
110 120 130 140 150
IMYNAKVVSH SVQDAAVEGG EGCLSVDREV PGYVVRHARV TVEYYNKEGE
160 170 180 190
KKKIRLKDFP AICVQHEIDH TNGVMFYDHI NMNDPWEIKD GMIIVK
Length:196
Mass (Da):22,047
Last modified:April 27, 2001 - v3
Checksum:iC5914BB3A92BF243
GO

Sequence cautioni

The sequence AAC41454.1 differs from that shown. Reason: Erroneous initiation.
The sequence AAK04658.1 differs from that shown. Reason: Erroneous initiation.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti86 – 861D → E in AAC41454. (PubMed:7773380)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L36907 Genomic DNA. Translation: AAC41454.1. Different initiation.
AE005176 Genomic DNA. Translation: AAK04658.1. Different initiation.
PIRiH86694.
RefSeqiNP_266716.1. NC_002662.1.

Genome annotation databases

EnsemblBacteriaiAAK04658; AAK04658; L154885.
GeneIDi1114179.
KEGGilla:L154885.
PATRICi22293378. VBILacLac136773_0598.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L36907 Genomic DNA. Translation: AAC41454.1 . Different initiation.
AE005176 Genomic DNA. Translation: AAK04658.1 . Different initiation.
PIRi H86694.
RefSeqi NP_266716.1. NC_002662.1.

3D structure databases

ProteinModelPortali Q48661.
SMRi Q48661. Positions 1-195.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 272623.L154885.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAK04658 ; AAK04658 ; L154885 .
GeneIDi 1114179.
KEGGi lla:L154885.
PATRICi 22293378. VBILacLac136773_0598.

Phylogenomic databases

eggNOGi COG0242.
HOGENOMi HOG000243507.
KOi K01462.
OMAi SQDPKIA.
OrthoDBi EOG6PZXGQ.

Enzyme and pathway databases

BioCyci LLAC272623:GHSH-617-MONOMER.

Family and domain databases

Gene3Di 3.90.45.10. 1 hit.
HAMAPi MF_00163. Pep_deformylase.
InterProi IPR000181. Fmet_deformylase.
IPR023635. Peptide_deformylase.
[Graphical view ]
PANTHERi PTHR10458. PTHR10458. 1 hit.
Pfami PF01327. Pep_deformylase. 1 hit.
[Graphical view ]
PIRSFi PIRSF004749. Pep_def. 1 hit.
PRINTSi PR01576. PDEFORMYLASE.
SUPFAMi SSF56420. SSF56420. 1 hit.
TIGRFAMsi TIGR00079. pept_deformyl. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Lactococcus lactis glyceraldehyde-3-phosphate dehydrogenase gene, gap: further evidence for strongly biased codon usage in glycolytic pathway genes."
    Cancilla M.R., Hillier A.J., Davidson B.E.
    Microbiology 141:1027-1036(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: LM0230.
  2. "The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403."
    Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J., Ehrlich S.D., Sorokin A.
    Genome Res. 11:731-753(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: IL1403.

Entry informationi

Entry nameiDEF_LACLA
AccessioniPrimary (citable) accession number: Q48661
Secondary accession number(s): Q9CI08
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: April 27, 2001
Last modified: October 1, 2014
This is version 100 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3