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Q48656

- AMPN_LACLL

UniProt

Q48656 - AMPN_LACLL

Protein

Aminopeptidase N

Gene

pepN

Organism
Lactococcus lactis subsp. lactis (Streptococcus lactis)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 81 (01 Oct 2014)
      Sequence version 2 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Aminopeptidase with broad substrate specificity to several peptides. It has more affinity for oligopeptides than for dipeptides. It plays an essential role in the metabolism, it may be involved in nitrogen supply or protein turnover.

    Catalytic activityi

    Release of an N-terminal amino acid, Xaa-|-Yaa- from a peptide, amide or arylamide. Xaa is preferably Ala, but may be most amino acids including Pro (slow action). When a terminal hydrophobic residue is followed by a prolyl residue, the two may be released as an intact Xaa-Pro dipeptide.

    Cofactori

    Binds 1 zinc ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei122 – 1221SubstrateBy similarity
    Metal bindingi294 – 2941Zinc; catalyticPROSITE-ProRule annotation
    Active sitei295 – 2951Proton acceptorPROSITE-ProRule annotation
    Metal bindingi298 – 2981Zinc; catalyticPROSITE-ProRule annotation
    Metal bindingi317 – 3171Zinc; catalyticPROSITE-ProRule annotation
    Sitei382 – 3821Transition state stabilizerBy similarity

    GO - Molecular functioni

    1. aminopeptidase activity Source: UniProtKB-KW
    2. metallopeptidase activity Source: UniProtKB-KW
    3. zinc ion binding Source: InterPro

    Keywords - Molecular functioni

    Aminopeptidase, Hydrolase, Metalloprotease, Protease

    Keywords - Ligandi

    Metal-binding, Zinc

    Protein family/group databases

    MEROPSiM01.002.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Aminopeptidase N (EC:3.4.11.2)
    Alternative name(s):
    Alanine aminopeptidase
    Lysyl aminopeptidase
    Short name:
    Lys-AP
    Gene namesi
    Name:pepN
    OrganismiLactococcus lactis subsp. lactis (Streptococcus lactis)
    Taxonomic identifieri1360 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus

    Subcellular locationi

    Cytoplasm
    Note: It may be secreted through an unknown mechanism.

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed
    Chaini2 – 849848Aminopeptidase NPRO_0000095074Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.

    Structurei

    3D structure databases

    ProteinModelPortaliQ48656.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni259 – 2635Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the peptidase M1 family.Curated

    Family and domain databases

    InterProiIPR024571. ERAP1-like_C_dom.
    IPR001930. Peptidase_M1.
    IPR014782. Peptidase_M1_N.
    [Graphical view]
    PANTHERiPTHR11533. PTHR11533. 1 hit.
    PfamiPF11838. ERAP1_C. 1 hit.
    PF01433. Peptidase_M1. 1 hit.
    [Graphical view]
    PRINTSiPR00756. ALADIPTASE.
    PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q48656-1 [UniParc]FASTAAdd to Basket

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    MTASVARFIE SFIPENYNLF LDINRSEKTF TGNVAITGEA IDNHISLHQK    50
    DLTINSVLLD NESLNFQMDD ANEAFHIELP ETGVLTIFIE FSGRITDNMT 100
    GIYPSYYTYN GEKKEIISTQ FEISHFAREA FPCVDEPEAK ATFDLSLKFD 150
    AEEGDTALSN MPEINSHLRE ETGVWTFETT PRMSTYLLAF GFGALHGKTA 200
    KTKNGTEVGV FATVAQAENS FDFALDIAVR VIEFYEDYFQ VKYPIPLSYH 250
    LALPDFSAGA MENWGLVTYR EVYLLVDENS SAASRQQVAL VVAHELAHQW 300
    FGNLVTMKWW DDLWLNESFA NMMEYVSVNA IEPSWNIFEG FPNKLGVPNA 350
    LQRDATDGVQ SVHMEVSHPD EINTLFDSAI VYAKGSRLMH MLRRWLGDEA 400
    FAKGLKAYFE KHQYNNTVGR DLWNALSEAS GKDVSSFMDT WLEQPGYPVV 450
    SAEVVDDTLI LSQKQFFIGE HEDKGRLWEI PLNTNWNGLP DTLSGERIEI 500
    PNYSQLATEN NGVLRLNTAN TAHYITDYQG QLLDNILEDF ANLDTVSKLQ 550
    ILQERRLLAE SGRISYASLV GLLDLVEKEE SFFLISQAKS QILAGLKRFI 600
    DEDTEAEVHY KALVRRQFQN DFERLGFDAK EGESDEDEMV RQTALSYLIE 650
    ADYQPTVLAA ANVFQAHKEN IESIPASIRG LVLINQMKQE NSLSLVEEYI 700
    NAYVATNDSN FRRQLTQALS YLKNQEGLDY VLGQLKDKNV VKPQDLYLWY 750
    MNFLSKSFAQ ETVWDWAKEN WEWIKAALGG DMSFDSFVNI PAGIFKNQER 800
    LDQYIAFFEP QTSDKALERN ILMGIKTIAA RVDLIEKEKA AVESALKDY 849
    Length:849
    Mass (Da):96,565
    Last modified:January 23, 2007 - v2
    Checksum:iE809F5437DFBD50F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D38040 Genomic DNA. Translation: BAA07234.1.
    PIRiJU0191.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D38040 Genomic DNA. Translation: BAA07234.1 .
    PIRi JU0191.

    3D structure databases

    ProteinModelPortali Q48656.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi M01.002.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR024571. ERAP1-like_C_dom.
    IPR001930. Peptidase_M1.
    IPR014782. Peptidase_M1_N.
    [Graphical view ]
    PANTHERi PTHR11533. PTHR11533. 1 hit.
    Pfami PF11838. ERAP1_C. 1 hit.
    PF01433. Peptidase_M1. 1 hit.
    [Graphical view ]
    PRINTSi PR00756. ALADIPTASE.
    PROSITEi PS00142. ZINC_PROTEASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning of lysyl-aminopeptidase gene from Lactococcus lactis."
      Tsukasaki F., Motoshima H., Minagawa E., Kaminogawa S.
      Submitted (APR-1996) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
      Strain: YRC001.

    Entry informationi

    Entry nameiAMPN_LACLL
    AccessioniPrimary (citable) accession number: Q48656
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 81 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3