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Q48656

- AMPN_LACLL

UniProt

Q48656 - AMPN_LACLL

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Protein

Aminopeptidase N

Gene

pepN

Organism
Lactococcus lactis subsp. lactis (Streptococcus lactis)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Aminopeptidase with broad substrate specificity to several peptides. It has more affinity for oligopeptides than for dipeptides. It plays an essential role in the metabolism, it may be involved in nitrogen supply or protein turnover.

Catalytic activityi

Release of an N-terminal amino acid, Xaa-|-Yaa- from a peptide, amide or arylamide. Xaa is preferably Ala, but may be most amino acids including Pro (slow action). When a terminal hydrophobic residue is followed by a prolyl residue, the two may be released as an intact Xaa-Pro dipeptide.

Cofactori

Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei122 – 1221SubstrateBy similarity
Metal bindingi294 – 2941Zinc; catalyticPROSITE-ProRule annotation
Active sitei295 – 2951Proton acceptorPROSITE-ProRule annotation
Metal bindingi298 – 2981Zinc; catalyticPROSITE-ProRule annotation
Metal bindingi317 – 3171Zinc; catalyticPROSITE-ProRule annotation
Sitei382 – 3821Transition state stabilizerBy similarity

GO - Molecular functioni

  1. aminopeptidase activity Source: UniProtKB-KW
  2. metallopeptidase activity Source: UniProtKB-KW
  3. zinc ion binding Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Aminopeptidase, Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiM01.A25.

Names & Taxonomyi

Protein namesi
Recommended name:
Aminopeptidase N (EC:3.4.11.2)
Alternative name(s):
Alanine aminopeptidase
Lysyl aminopeptidase
Short name:
Lys-AP
Gene namesi
Name:pepN
OrganismiLactococcus lactis subsp. lactis (Streptococcus lactis)
Taxonomic identifieri1360 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus

Subcellular locationi

Cytoplasm
Note: It may be secreted through an unknown mechanism.

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed
Chaini2 – 849848Aminopeptidase NPRO_0000095074Add
BLAST

Interactioni

Subunit structurei

Monomer.

Structurei

3D structure databases

ProteinModelPortaliQ48656.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni259 – 2635Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the peptidase M1 family.Curated

Family and domain databases

InterProiIPR024571. ERAP1-like_C_dom.
IPR001930. Peptidase_M1.
IPR014782. Peptidase_M1_N.
[Graphical view]
PANTHERiPTHR11533. PTHR11533. 1 hit.
PfamiPF11838. ERAP1_C. 1 hit.
PF01433. Peptidase_M1. 1 hit.
[Graphical view]
PRINTSiPR00756. ALADIPTASE.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q48656-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTASVARFIE SFIPENYNLF LDINRSEKTF TGNVAITGEA IDNHISLHQK
60 70 80 90 100
DLTINSVLLD NESLNFQMDD ANEAFHIELP ETGVLTIFIE FSGRITDNMT
110 120 130 140 150
GIYPSYYTYN GEKKEIISTQ FEISHFAREA FPCVDEPEAK ATFDLSLKFD
160 170 180 190 200
AEEGDTALSN MPEINSHLRE ETGVWTFETT PRMSTYLLAF GFGALHGKTA
210 220 230 240 250
KTKNGTEVGV FATVAQAENS FDFALDIAVR VIEFYEDYFQ VKYPIPLSYH
260 270 280 290 300
LALPDFSAGA MENWGLVTYR EVYLLVDENS SAASRQQVAL VVAHELAHQW
310 320 330 340 350
FGNLVTMKWW DDLWLNESFA NMMEYVSVNA IEPSWNIFEG FPNKLGVPNA
360 370 380 390 400
LQRDATDGVQ SVHMEVSHPD EINTLFDSAI VYAKGSRLMH MLRRWLGDEA
410 420 430 440 450
FAKGLKAYFE KHQYNNTVGR DLWNALSEAS GKDVSSFMDT WLEQPGYPVV
460 470 480 490 500
SAEVVDDTLI LSQKQFFIGE HEDKGRLWEI PLNTNWNGLP DTLSGERIEI
510 520 530 540 550
PNYSQLATEN NGVLRLNTAN TAHYITDYQG QLLDNILEDF ANLDTVSKLQ
560 570 580 590 600
ILQERRLLAE SGRISYASLV GLLDLVEKEE SFFLISQAKS QILAGLKRFI
610 620 630 640 650
DEDTEAEVHY KALVRRQFQN DFERLGFDAK EGESDEDEMV RQTALSYLIE
660 670 680 690 700
ADYQPTVLAA ANVFQAHKEN IESIPASIRG LVLINQMKQE NSLSLVEEYI
710 720 730 740 750
NAYVATNDSN FRRQLTQALS YLKNQEGLDY VLGQLKDKNV VKPQDLYLWY
760 770 780 790 800
MNFLSKSFAQ ETVWDWAKEN WEWIKAALGG DMSFDSFVNI PAGIFKNQER
810 820 830 840
LDQYIAFFEP QTSDKALERN ILMGIKTIAA RVDLIEKEKA AVESALKDY
Length:849
Mass (Da):96,565
Last modified:January 23, 2007 - v2
Checksum:iE809F5437DFBD50F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D38040 Genomic DNA. Translation: BAA07234.1.
PIRiJU0191.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D38040 Genomic DNA. Translation: BAA07234.1 .
PIRi JU0191.

3D structure databases

ProteinModelPortali Q48656.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi M01.A25.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR024571. ERAP1-like_C_dom.
IPR001930. Peptidase_M1.
IPR014782. Peptidase_M1_N.
[Graphical view ]
PANTHERi PTHR11533. PTHR11533. 1 hit.
Pfami PF11838. ERAP1_C. 1 hit.
PF01433. Peptidase_M1. 1 hit.
[Graphical view ]
PRINTSi PR00756. ALADIPTASE.
PROSITEi PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning of lysyl-aminopeptidase gene from Lactococcus lactis."
    Tsukasaki F., Motoshima H., Minagawa E., Kaminogawa S.
    Submitted (APR-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
    Strain: YRC001.

Entry informationi

Entry nameiAMPN_LACLL
AccessioniPrimary (citable) accession number: Q48656
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 82 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3