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Q48656

- AMPN_LACLL

UniProt

Q48656 - AMPN_LACLL

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Protein

Aminopeptidase N

Gene
pepN
Organism
Lactococcus lactis subsp. lactis (Streptococcus lactis)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Aminopeptidase with broad substrate specificity to several peptides. It has more affinity for oligopeptides than for dipeptides. It plays an essential role in the metabolism, it may be involved in nitrogen supply or protein turnover.

Catalytic activityi

Release of an N-terminal amino acid, Xaa-|-Yaa- from a peptide, amide or arylamide. Xaa is preferably Ala, but may be most amino acids including Pro (slow action). When a terminal hydrophobic residue is followed by a prolyl residue, the two may be released as an intact Xaa-Pro dipeptide.

Cofactori

Binds 1 zinc ion per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei122 – 1221Substrate By similarity
Metal bindingi294 – 2941Zinc; catalytic By similarity
Active sitei295 – 2951Proton acceptor By similarity
Metal bindingi298 – 2981Zinc; catalytic By similarity
Metal bindingi317 – 3171Zinc; catalytic By similarity
Sitei382 – 3821Transition state stabilizer By similarity

GO - Molecular functioni

  1. aminopeptidase activity Source: UniProtKB-KW
  2. metallopeptidase activity Source: UniProtKB-KW
  3. zinc ion binding Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Aminopeptidase, Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiM01.002.

Names & Taxonomyi

Protein namesi
Recommended name:
Aminopeptidase N (EC:3.4.11.2)
Alternative name(s):
Alanine aminopeptidase
Lysyl aminopeptidase
Short name:
Lys-AP
Gene namesi
Name:pepN
OrganismiLactococcus lactis subsp. lactis (Streptococcus lactis)
Taxonomic identifieri1360 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus

Subcellular locationi

Cytoplasm
Note: It may be secreted through an unknown mechanism.

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed
Chaini2 – 849848Aminopeptidase NPRO_0000095074Add
BLAST

Interactioni

Subunit structurei

Monomer.

Structurei

3D structure databases

ProteinModelPortaliQ48656.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni259 – 2635Substrate binding By similarity

Sequence similaritiesi

Belongs to the peptidase M1 family.

Family and domain databases

InterProiIPR024571. ERAP1-like_C_dom.
IPR001930. Peptidase_M1.
IPR014782. Peptidase_M1_N.
[Graphical view]
PANTHERiPTHR11533. PTHR11533. 1 hit.
PfamiPF11838. ERAP1_C. 1 hit.
PF01433. Peptidase_M1. 1 hit.
[Graphical view]
PRINTSiPR00756. ALADIPTASE.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q48656-1 [UniParc]FASTAAdd to Basket

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MTASVARFIE SFIPENYNLF LDINRSEKTF TGNVAITGEA IDNHISLHQK    50
DLTINSVLLD NESLNFQMDD ANEAFHIELP ETGVLTIFIE FSGRITDNMT 100
GIYPSYYTYN GEKKEIISTQ FEISHFAREA FPCVDEPEAK ATFDLSLKFD 150
AEEGDTALSN MPEINSHLRE ETGVWTFETT PRMSTYLLAF GFGALHGKTA 200
KTKNGTEVGV FATVAQAENS FDFALDIAVR VIEFYEDYFQ VKYPIPLSYH 250
LALPDFSAGA MENWGLVTYR EVYLLVDENS SAASRQQVAL VVAHELAHQW 300
FGNLVTMKWW DDLWLNESFA NMMEYVSVNA IEPSWNIFEG FPNKLGVPNA 350
LQRDATDGVQ SVHMEVSHPD EINTLFDSAI VYAKGSRLMH MLRRWLGDEA 400
FAKGLKAYFE KHQYNNTVGR DLWNALSEAS GKDVSSFMDT WLEQPGYPVV 450
SAEVVDDTLI LSQKQFFIGE HEDKGRLWEI PLNTNWNGLP DTLSGERIEI 500
PNYSQLATEN NGVLRLNTAN TAHYITDYQG QLLDNILEDF ANLDTVSKLQ 550
ILQERRLLAE SGRISYASLV GLLDLVEKEE SFFLISQAKS QILAGLKRFI 600
DEDTEAEVHY KALVRRQFQN DFERLGFDAK EGESDEDEMV RQTALSYLIE 650
ADYQPTVLAA ANVFQAHKEN IESIPASIRG LVLINQMKQE NSLSLVEEYI 700
NAYVATNDSN FRRQLTQALS YLKNQEGLDY VLGQLKDKNV VKPQDLYLWY 750
MNFLSKSFAQ ETVWDWAKEN WEWIKAALGG DMSFDSFVNI PAGIFKNQER 800
LDQYIAFFEP QTSDKALERN ILMGIKTIAA RVDLIEKEKA AVESALKDY 849
Length:849
Mass (Da):96,565
Last modified:January 23, 2007 - v2
Checksum:iE809F5437DFBD50F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D38040 Genomic DNA. Translation: BAA07234.1.
PIRiJU0191.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D38040 Genomic DNA. Translation: BAA07234.1 .
PIRi JU0191.

3D structure databases

ProteinModelPortali Q48656.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi M01.002.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR024571. ERAP1-like_C_dom.
IPR001930. Peptidase_M1.
IPR014782. Peptidase_M1_N.
[Graphical view ]
PANTHERi PTHR11533. PTHR11533. 1 hit.
Pfami PF11838. ERAP1_C. 1 hit.
PF01433. Peptidase_M1. 1 hit.
[Graphical view ]
PRINTSi PR00756. ALADIPTASE.
PROSITEi PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning of lysyl-aminopeptidase gene from Lactococcus lactis."
    Tsukasaki F., Motoshima H., Minagawa E., Kaminogawa S.
    Submitted (APR-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
    Strain: YRC001.

Entry informationi

Entry nameiAMPN_LACLL
AccessioniPrimary (citable) accession number: Q48656
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 23, 2007
Last modified: May 14, 2014
This is version 80 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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