Q48656 (AMPN_LACLL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aminopeptidase N
EC=3.4.11.2

Alternative name(s):
Alanine aminopeptidase
Lysyl aminopeptidase
Short name=Lys-AP

Gene names

Name:

pepN

Organism

Lactococcus lactis subsp. lactis (Streptococcus lactis)

Taxonomic identifier

1360 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Lactococcus

Protein attributes

Sequence length	849 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Aminopeptidase with broad substrate specificity to several peptides. It has more affinity for oligopeptides than for dipeptides. It plays an essential role in the metabolism, it may be involved in nitrogen supply or protein turnover.
Catalytic activity	Release of an N-terminal amino acid, Xaa-|-Yaa- from a peptide, amide or arylamide. Xaa is preferably Ala, but may be most amino acids including Pro (slow action). When a terminal hydrophobic residue is followed by a prolyl residue, the two may be released as an intact Xaa-Pro dipeptide.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Monomer.
Subcellular location	Cytoplasm. Note: It may be secreted through an unknown mechanism.
Sequence similarities	Belongs to the peptidase M1 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding Zinc
Molecular function	Aminopeptidase Hydrolase Metalloprotease Protease
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW metallopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 849	848	Aminopeptidase N		PRO_0000095074
Regions
	Region	259 – 263	5	Substrate binding By similarity
Sites
	Active site	295	1	Proton acceptor By similarity
	Metal binding	294	1	Zinc; catalytic By similarity
	Metal binding	298	1	Zinc; catalytic By similarity
	Metal binding	317	1	Zinc; catalytic By similarity
	Binding site	122	1	Substrate By similarity
	Site	382	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q48656 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: E809F5437DFBD50F	FASTA	849	96,565
	10 20 30 40 50 60 MTASVARFIE SFIPENYNLF LDINRSEKTF TGNVAITGEA IDNHISLHQK DLTINSVLLD 70 80 90 100 110 120 NESLNFQMDD ANEAFHIELP ETGVLTIFIE FSGRITDNMT GIYPSYYTYN GEKKEIISTQ 130 140 150 160 170 180 FEISHFAREA FPCVDEPEAK ATFDLSLKFD AEEGDTALSN MPEINSHLRE ETGVWTFETT 190 200 210 220 230 240 PRMSTYLLAF GFGALHGKTA KTKNGTEVGV FATVAQAENS FDFALDIAVR VIEFYEDYFQ 250 260 270 280 290 300 VKYPIPLSYH LALPDFSAGA MENWGLVTYR EVYLLVDENS SAASRQQVAL VVAHELAHQW 310 320 330 340 350 360 FGNLVTMKWW DDLWLNESFA NMMEYVSVNA IEPSWNIFEG FPNKLGVPNA LQRDATDGVQ 370 380 390 400 410 420 SVHMEVSHPD EINTLFDSAI VYAKGSRLMH MLRRWLGDEA FAKGLKAYFE KHQYNNTVGR 430 440 450 460 470 480 DLWNALSEAS GKDVSSFMDT WLEQPGYPVV SAEVVDDTLI LSQKQFFIGE HEDKGRLWEI 490 500 510 520 530 540 PLNTNWNGLP DTLSGERIEI PNYSQLATEN NGVLRLNTAN TAHYITDYQG QLLDNILEDF 550 560 570 580 590 600 ANLDTVSKLQ ILQERRLLAE SGRISYASLV GLLDLVEKEE SFFLISQAKS QILAGLKRFI 610 620 630 640 650 660 DEDTEAEVHY KALVRRQFQN DFERLGFDAK EGESDEDEMV RQTALSYLIE ADYQPTVLAA 670 680 690 700 710 720 ANVFQAHKEN IESIPASIRG LVLINQMKQE NSLSLVEEYI NAYVATNDSN FRRQLTQALS 730 740 750 760 770 780 YLKNQEGLDY VLGQLKDKNV VKPQDLYLWY MNFLSKSFAQ ETVWDWAKEN WEWIKAALGG 790 800 810 820 830 840 DMSFDSFVNI PAGIFKNQER LDQYIAFFEP QTSDKALERN ILMGIKTIAA RVDLIEKEKA AVESALKDY

References

[1]	"Cloning of lysyl-aminopeptidase gene from Lactococcus lactis." Tsukasaki F., Motoshima H., Minagawa E., Kaminogawa S. Submitted (APR-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE. Strain: YRC001.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D38040 Genomic DNA. Translation: BAA07234.1.
PIR	JU0191.
3D structure databases
ProteinModelPortal	Q48656.
ModBase	Search...
Protein family/group databases
MEROPS	M01.002.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001930. Peptidase_M1. IPR014782. Peptidase_M1_N. [Graphical view]
PANTHER	PTHR11533. Peptidase_M1. 1 hit.
Pfam	PF01433. Peptidase_M1. 1 hit. [Graphical view]
PRINTS	PR00756. ALADIPTASE.
PROSITE	PS00142. ZINC_PROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPN_LACLL

Accession

Primary (citable) accession number: Q48656

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	January 23, 2007
Last modified:	November 16, 2011
This is version 69 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program