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Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene

glnD

Organism
Colwellia psychrerythraea (strain 34H / ATCC BAA-681) (Vibrio psychroerythus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity.UniRule annotation

GO - Molecular functioni

  1. [protein-PII] uridylyltransferase activity Source: TIGR
  2. amino acid binding Source: InterPro
  3. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. nitrogen compound metabolic process Source: InterPro
  2. regulation of nitrogen utilization Source: TIGR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciCPSY167879:GI48-1549-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
Short name:
UTase/URUniRule annotation
Alternative name(s):
Bifunctional [protein-PII] modification enzymeUniRule annotation
Bifunctional nitrogen sensor proteinUniRule annotation
Including the following 2 domains:
[Protein-PII] uridylyltransferaseUniRule annotation (EC:2.7.7.59UniRule annotation)
Short name:
PII uridylyltransferaseUniRule annotation
Short name:
UTaseUniRule annotation
[Protein-PII]-UMP uridylyl-removing enzymeUniRule annotation (EC:3.1.4.-UniRule annotation)
Short name:
URUniRule annotation
Gene namesi
Name:glnDUniRule annotation
Ordered Locus Names:CPS_1549
OrganismiColwellia psychrerythraea (strain 34H / ATCC BAA-681) (Vibrio psychroerythus)
Taxonomic identifieri167879 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesColwelliaceaeColwellia
ProteomesiUP000000547 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 878878Bifunctional uridylyltransferase/uridylyl-removing enzymePRO_0000192729Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi167879.CPS_1549.

Structurei

3D structure databases

ProteinModelPortaliQ485H4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini460 – 567108HDUniRule annotationAdd
BLAST
Domaini701 – 77979ACT 1UniRule annotationAdd
BLAST
Domaini809 – 87870ACT 2UniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 342342UridylyltransferaseAdd
BLAST
Regioni343 – 700358Uridylyl-removingAdd
BLAST

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.UniRule annotation
Contains 2 ACT domains.UniRule annotation
Contains 1 HD domain.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG2844.
HOGENOMiHOG000261778.
KOiK00990.
OMAiSLWNNWK.
OrthoDBiEOG6CCH44.

Family and domain databases

Gene3Di1.10.3210.10. 1 hit.
HAMAPiMF_00277. PII_uridylyl_transf.
InterProiIPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view]
PfamiPF01842. ACT. 1 hit.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiPS51671. ACT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q485H4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSHPVSVHI VPSHFINQLA ISADFLSTRD ICQLSQSFNT WLKDVFIEND
60 70 80 90 100
INDLLSARAV FVDAILKKLW CQHHLDEFQI TLIAVGGYGR GELHPQSDVD
110 120 130 140 150
ILLLTQEEVD LELEEKISSF ITQLWDIKLD IGHSVRSIKE CLKQAVKEVT
160 170 180 190 200
VATNLMEMRQ VAGNETLTQQ LTPLLSEDVF WTSEKFFIAK CKEQEARHQQ
210 220 230 240 250
YRGAAYTLEP NLKANPGGLR DIQTIAWVAK RHFSADSLEE LVEHDYLYPN
260 270 280 290 300
EFFELLESQD YLWRMRFALH FVAGRSENRL LFDYQADVAK MMGFGDEGKA
310 320 330 340 350
PVERMMKRFF RIIARVTELN TMLLQHFEQA IIKKPELSNI SIINQDFELV
360 370 380 390 400
DKLINTRNDR IFMRPVKMIE MFLIIAQEPG IKGIHSHTMR LMRNARRRLI
410 420 430 440 450
SGLIDYAECR RMFMAIIRHP RGLGLALTLM HRHSILSSYL PLWRNIAGQM
460 470 480 490 500
QFDLFHAYSV DEHSYRVIKN LHQFSQKEHN HKFPLCSKIV QKIRKPEVLY
510 520 530 540 550
LAGFFHDIGK GRGGDHAKLG AVDALTFCLS HQLSKHDSNM VAWLVEHHLL
560 570 580 590 600
MSVTAQRRDI NDENVIRTFG EIVRDEAHLN YLYCLTVADM RGTNESLWNN
610 620 630 640 650
WKANLLEELY FNTLSAFRHG LEKPVEVRSK IRENQQQALA LLNENNVDEQ
660 670 680 690 700
SIKALWREFR IDYFLRYSPE QIARQCQNIV EHDREKPLVL ISPIPYRGGT
710 720 730 740 750
EVFIFTKEKN NTFASTVSFL VTKKLSIHDA KIITTKTGYT VNTFVVLDSR
760 770 780 790 800
NKPLRERFYT KEMSQALVDR LQQVNICELP EPKLARHMKK FKVPLRVNFI
810 820 830 840 850
KIHAKNRTMI EIIALDRPGL LSNISQVFLE ARVNIHSAKI TTFGEKADDV
860 870
FTISTEEDDA LTTQEKEALA LRLTQEID
Length:878
Mass (Da):101,844
Last modified:September 12, 2005 - v1
Checksum:i90026DD7C373EF21
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000083 Genomic DNA. Translation: AAZ26298.1.
RefSeqiYP_268291.1. NC_003910.7.

Genome annotation databases

EnsemblBacteriaiAAZ26298; AAZ26298; CPS_1549.
KEGGicps:CPS_1549.
PATRICi21466307. VBIColPsy94388_1405.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000083 Genomic DNA. Translation: AAZ26298.1.
RefSeqiYP_268291.1. NC_003910.7.

3D structure databases

ProteinModelPortaliQ485H4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi167879.CPS_1549.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAZ26298; AAZ26298; CPS_1549.
KEGGicps:CPS_1549.
PATRICi21466307. VBIColPsy94388_1405.

Phylogenomic databases

eggNOGiCOG2844.
HOGENOMiHOG000261778.
KOiK00990.
OMAiSLWNNWK.
OrthoDBiEOG6CCH44.

Enzyme and pathway databases

BioCyciCPSY167879:GI48-1549-MONOMER.

Family and domain databases

Gene3Di1.10.3210.10. 1 hit.
HAMAPiMF_00277. PII_uridylyl_transf.
InterProiIPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view]
PfamiPF01842. ACT. 1 hit.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiPS51671. ACT. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 34H / ATCC BAA-681.

Entry informationi

Entry nameiGLND_COLP3
AccessioniPrimary (citable) accession number: Q485H4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 7, 2005
Last sequence update: September 12, 2005
Last modified: March 31, 2015
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.