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Q485H4

- GLND_COLP3

UniProt

Q485H4 - GLND_COLP3

Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene

glnD

Organism
Colwellia psychrerythraea (strain 34H / ATCC BAA-681) (Vibrio psychroerythus)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 65 (01 Oct 2014)
      Sequence version 1 (13 Sep 2005)
      Previous versions | rss
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    Functioni

    Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism.UniRule annotation

    Catalytic activityi

    UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
    Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Enzyme regulationi

    Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity.UniRule annotation

    GO - Molecular functioni

    1. [protein-PII] uridylyltransferase activity Source: UniProtKB-HAMAP
    2. amino acid binding Source: InterPro
    3. metal ion binding Source: InterPro
    4. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. nitrogen compound metabolic process Source: InterPro
    2. regulation of nitrogen utilization Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Hydrolase, Nucleotidyltransferase, Transferase

    Keywords - Ligandi

    Magnesium

    Enzyme and pathway databases

    BioCyciCPSY167879:GI48-1549-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
    Short name:
    UTase/URUniRule annotation
    Alternative name(s):
    Bifunctional [protein-PII] modification enzymeUniRule annotation
    Bifunctional nitrogen sensor proteinUniRule annotation
    Including the following 2 domains:
    [Protein-PII] uridylyltransferaseUniRule annotation (EC:2.7.7.59UniRule annotation)
    Short name:
    PII uridylyltransferaseUniRule annotation
    Short name:
    UTaseUniRule annotation
    [Protein-PII]-UMP uridylyl-removing enzymeUniRule annotation (EC:3.1.4.-UniRule annotation)
    Short name:
    URUniRule annotation
    Gene namesi
    Name:glnDUniRule annotation
    Ordered Locus Names:CPS_1549
    OrganismiColwellia psychrerythraea (strain 34H / ATCC BAA-681) (Vibrio psychroerythus)
    Taxonomic identifieri167879 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesColwelliaceaeColwellia
    ProteomesiUP000000547: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 878878Bifunctional uridylyltransferase/uridylyl-removing enzymePRO_0000192729Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi167879.CPS_1549.

    Structurei

    3D structure databases

    ProteinModelPortaliQ485H4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini460 – 567108HDUniRule annotationAdd
    BLAST
    Domaini701 – 77979ACT 1UniRule annotationAdd
    BLAST
    Domaini809 – 87870ACT 2UniRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 342342UridylyltransferaseAdd
    BLAST
    Regioni343 – 700358Uridylyl-removingAdd
    BLAST

    Domaini

    Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing.UniRule annotation

    Sequence similaritiesi

    Belongs to the GlnD family.UniRule annotation
    Contains 2 ACT domains.UniRule annotation
    Contains 1 HD domain.UniRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG2844.
    HOGENOMiHOG000261778.
    KOiK00990.
    OMAiHHLLMSV.
    OrthoDBiEOG6CCH44.

    Family and domain databases

    Gene3Di1.10.3210.10. 1 hit.
    HAMAPiMF_00277. PII_uridylyl_transf.
    InterProiIPR002912. ACT_dom.
    IPR010043. GlnD_Uridyltrans.
    IPR003607. HD/PDEase_dom.
    IPR006674. HD_domain.
    IPR002934. Nucleotidyltransferase.
    IPR013546. PII_UdlTrfase/GS_AdlTrfase.
    [Graphical view]
    PfamiPF01842. ACT. 1 hit.
    PF08335. GlnD_UR_UTase. 1 hit.
    PF01966. HD. 1 hit.
    PF01909. NTP_transf_2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
    SMARTiSM00471. HDc. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
    PROSITEiPS51671. ACT. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q485H4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTSHPVSVHI VPSHFINQLA ISADFLSTRD ICQLSQSFNT WLKDVFIEND    50
    INDLLSARAV FVDAILKKLW CQHHLDEFQI TLIAVGGYGR GELHPQSDVD 100
    ILLLTQEEVD LELEEKISSF ITQLWDIKLD IGHSVRSIKE CLKQAVKEVT 150
    VATNLMEMRQ VAGNETLTQQ LTPLLSEDVF WTSEKFFIAK CKEQEARHQQ 200
    YRGAAYTLEP NLKANPGGLR DIQTIAWVAK RHFSADSLEE LVEHDYLYPN 250
    EFFELLESQD YLWRMRFALH FVAGRSENRL LFDYQADVAK MMGFGDEGKA 300
    PVERMMKRFF RIIARVTELN TMLLQHFEQA IIKKPELSNI SIINQDFELV 350
    DKLINTRNDR IFMRPVKMIE MFLIIAQEPG IKGIHSHTMR LMRNARRRLI 400
    SGLIDYAECR RMFMAIIRHP RGLGLALTLM HRHSILSSYL PLWRNIAGQM 450
    QFDLFHAYSV DEHSYRVIKN LHQFSQKEHN HKFPLCSKIV QKIRKPEVLY 500
    LAGFFHDIGK GRGGDHAKLG AVDALTFCLS HQLSKHDSNM VAWLVEHHLL 550
    MSVTAQRRDI NDENVIRTFG EIVRDEAHLN YLYCLTVADM RGTNESLWNN 600
    WKANLLEELY FNTLSAFRHG LEKPVEVRSK IRENQQQALA LLNENNVDEQ 650
    SIKALWREFR IDYFLRYSPE QIARQCQNIV EHDREKPLVL ISPIPYRGGT 700
    EVFIFTKEKN NTFASTVSFL VTKKLSIHDA KIITTKTGYT VNTFVVLDSR 750
    NKPLRERFYT KEMSQALVDR LQQVNICELP EPKLARHMKK FKVPLRVNFI 800
    KIHAKNRTMI EIIALDRPGL LSNISQVFLE ARVNIHSAKI TTFGEKADDV 850
    FTISTEEDDA LTTQEKEALA LRLTQEID 878
    Length:878
    Mass (Da):101,844
    Last modified:September 13, 2005 - v1
    Checksum:i90026DD7C373EF21
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000083 Genomic DNA. Translation: AAZ26298.1.
    RefSeqiYP_268291.1. NC_003910.7.

    Genome annotation databases

    EnsemblBacteriaiAAZ26298; AAZ26298; CPS_1549.
    GeneIDi3520548.
    KEGGicps:CPS_1549.
    PATRICi21466307. VBIColPsy94388_1405.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000083 Genomic DNA. Translation: AAZ26298.1 .
    RefSeqi YP_268291.1. NC_003910.7.

    3D structure databases

    ProteinModelPortali Q485H4.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 167879.CPS_1549.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAZ26298 ; AAZ26298 ; CPS_1549 .
    GeneIDi 3520548.
    KEGGi cps:CPS_1549.
    PATRICi 21466307. VBIColPsy94388_1405.

    Phylogenomic databases

    eggNOGi COG2844.
    HOGENOMi HOG000261778.
    KOi K00990.
    OMAi HHLLMSV.
    OrthoDBi EOG6CCH44.

    Enzyme and pathway databases

    BioCyci CPSY167879:GI48-1549-MONOMER.

    Family and domain databases

    Gene3Di 1.10.3210.10. 1 hit.
    HAMAPi MF_00277. PII_uridylyl_transf.
    InterProi IPR002912. ACT_dom.
    IPR010043. GlnD_Uridyltrans.
    IPR003607. HD/PDEase_dom.
    IPR006674. HD_domain.
    IPR002934. Nucleotidyltransferase.
    IPR013546. PII_UdlTrfase/GS_AdlTrfase.
    [Graphical view ]
    Pfami PF01842. ACT. 1 hit.
    PF08335. GlnD_UR_UTase. 1 hit.
    PF01966. HD. 1 hit.
    PF01909. NTP_transf_2. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF006288. PII_uridyltransf. 1 hit.
    SMARTi SM00471. HDc. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR01693. UTase_glnD. 1 hit.
    PROSITEi PS51671. ACT. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 34H / ATCC BAA-681.

    Entry informationi

    Entry nameiGLND_COLP3
    AccessioniPrimary (citable) accession number: Q485H4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 8, 2005
    Last sequence update: September 13, 2005
    Last modified: October 1, 2014
    This is version 65 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3