Q485F5 (LPXB_COLP3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lipid-A-disaccharide synthase
EC=2.4.1.182

Gene names

Name:	lpxB
Ordered Locus Names:	CPS_1568

Organism

Colwellia psychrerythraea (strain 34H / ATCC BAA-681) (Vibrio psychroerythus) [Complete proteome] [HAMAP]

Taxonomic identifier

167879 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Colwelliaceae › Colwellia › Colwellia psychrerythraea

Protein attributes

Sequence length	393 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Condensation of UDP-2,3-diacylglucosamine and 2,3-diacylglucosamine-1-phosphate to form lipid A disaccharide, a precursor of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell By similarity. HAMAP-Rule MF_00392
Catalytic activity	UDP-2,3-bis(3-hydroxytetradecanoyl)glucosamine + 2,3-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl 1-phosphate = UDP + 2,3-bis(3-hydroxytetradecanoyl)-D-glucosaminyl-1,6-beta-D-2,3-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl 1-phosphate. HAMAP-Rule MF_00392
Pathway	Glycolipid biosynthesis; lipid IV(A) biosynthesis; lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine: step 5/6. HAMAP-Rule MF_00392
Sequence similarities	Belongs to the LpxB family.

Ontologies

Keywords
Biological process	Lipid A biosynthesis Lipid biosynthesis Lipid metabolism
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	lipid A biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	lipid-A-disaccharide synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 393	393	Lipid-A-disaccharide synthase HAMAP-Rule MF_00392		PRO_0000255175

Sequences

	Sequence		Length	Mass (Da)
	Q485F5 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 380EDA95FF7BB4CC	FASTA	393	43,815
	10 20 30 40 50 60 MTTQNQNTHQ QTVFAMVVGE HSGDTLGAGL ITSLRQTHPH AKFIGIGGPK MLALGFESLF 70 80 90 100 110 120 AMDELSVMGL VEVLGRIRRL LHVRKTLTDF FITNKPDVFI GIDAPDFNIG LELKLKVKGI 130 140 150 160 170 180 KTVHYVSPSV WAWREKRIFK IAKATDMVLA LLPFEKAFYD KHNVPCTFVG HPLADDIPMQ 190 200 210 220 230 240 SDKVLARDKL GLAQDKKILA LMPGSRGGEL SRLLEDFFES AKQLQAQDSE LLFVAPMISE 250 260 270 280 290 300 QRANQFNALK AELAPDLDIE IVLNQTQQVM AASDCLLTAS GTVTLEAALI KRPMVICYKF 310 320 330 340 350 360 SPITFFLGRR FVKLKWFSLP NLLTNKSLVP ELLQKDVCPE NIVPLVKERL YQDQSQLNDS 370 380 390 FTAIHQQLKC DASKQAAKAV LDVLSSKLLS NNK

References

[1]

"The psychrophilic lifestyle as revealed by the genome sequence of Colwellia psychrerythraea 34H through genomic and proteomic analyses." Methe B.A., Nelson K.E., Deming J.W., Momen B., Melamud E., Zhang X., Moult J., Madupu R., Nelson W.C., Dodson R.J., Brinkac L.M., Daugherty S.C., Durkin A.S., DeBoy R.T., Kolonay J.F., Sullivan S.A., Zhou L., Davidsen T.M. , Wu M., Huston A.L., Lewis M., Weaver B., Weidman J.F., Khouri H., Utterback T.R., Feldblyum T.V., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 102:10913-10918(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 34H / ATCC BAA-681.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000083 Genomic DNA. Translation: AAZ28777.1.
RefSeq	YP_268310.1. NC_003910.7.
3D structure databases
ProteinModelPortal	Q485F5.
ModBase	Search...
Protein-protein interaction databases
STRING	167879.CPS_1568.
Protein family/group databases
CAZy	GT19. Glycosyltransferase Family 19.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAZ28777; AAZ28777; CPS_1568.
GeneID	3523039.
KEGG	cps:CPS_1568.
PATRIC	21466341. VBIColPsy94388_1422.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0763.
HOGENOM	HOG000018003.
KO	K00748.
OMA	PTVWAWR.
ProtClustDB	PRK00025.
Enzyme and pathway databases
BioCyc	CPSY167879:GI48-1649-MONOMER.
UniPathway	UPA00359; UER00481.
Family and domain databases
HAMAP	MF_00392. LpxB.
InterPro	IPR003835. Glyco_trans_19. [Graphical view]
Pfam	PF02684. LpxB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00215. lpxB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LPXB_COLP3

Accession

Primary (citable) accession number: Q485F5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	September 13, 2005
Last modified:	May 1, 2013
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program