Reviewed, UniProtKB/Swiss-Prot Q48543 (PEPC_LACDL)

Names and origin

Protein names

Recommended name:
    Aminopeptidase C
    EC=3.4.22.40
Alternative name(s):
    Bleomycin hydrolase

Gene names

Name:

pepC

Organism

Lactobacillus delbrueckii subsp. lactis

Taxonomic identifier

29397 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Lactobacillaceae › Lactobacillus

Protein attributes

Sequence length	449 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Inactivates bleomycin B2 (a cytotoxic glycometallopeptide) by hydrolysis of a carboxyamide bond of beta-aminoalanine, but also shows general aminopeptidase activity. The specificity varies somewhat with source, but amino acid arylamides of Met, Leu and Ala are preferred.
Subunit structure	Homohexamer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the peptidase C1 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Aminopeptidase Hydrolase Protease Thiol protease
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW cysteine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 449	449	Aminopeptidase C		PRO_0000050590
Sites
	Active site	70	1	By similarity
	Active site	364	1	By similarity
	Active site	385	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q48543-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 6E825BBAD53CE0D8	FASTA	449	50,903
	10 20 30 40 50 60 MSKEISFDTI EDFTSNLSKH PAYGVAANAA QTNGIFKASQ STQSKVDLDP TFSVEIDTGS 70 80 90 100 110 120 VTNQKQSGRC WMFSALNTMR HSIQKEFKLK GFELSQSYTF FWDKFEKSNF FFENVIGSAD 130 140 150 160 170 180 KPLGDRKVSF LFATPQSDGG QWDMLCGLIE KYGIVPKKVY PETANSENSR ALNDTLNTML 190 200 210 220 230 240 RKGGLELRAL VNEGKSTEEV EAHKAELLDA IFRMLATSLG LPPKSFNFEY TDDDGNYHID 250 260 270 280 290 300 KDITPQDFFK KYVGWDLENY ISVINGPTAD KPYNKVFSVE YLGNVVGGRQ VRHLNLELSK 310 320 330 340 350 360 FKELIINQLK QGEVVWFGSD VSKGGDREAG LLDTKIYQRD QLFDYDFSMS KADRLDSGES 370 380 390 400 410 420 MMNHAMVITA VDLVDDKPTK WKIENSWGDK SGFKGYFVMS DEWFDQFVYQ AVLNKAFLPE 430 440 DVKKAYDEGK ENPIELLPWD PMGALAFDF

References

[1]	"Cloning and nucleotide sequence analysis of the Lactobacillus delbrueckii ssp. lactis DSM7290 cysteine aminopeptidase gene pepC." Klein J., Henrich B., Plapp R. FEMS Microbiol. Lett. 124:291-299(1994) [PubMed: 7851736] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DSM 7290.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X80643 Genomic DNA. Translation: CAA56689.1.
PIR	S52865.
3D structure databases
HSSP	HSSP built from PDB template 2CB5 based on UniProtKB Q13867.
ModBase	Search...
Protein family/group databases
MEROPS	C01.086.
Enzyme and pathway databases
BRENDA	3.4.22.40. 270587.
Family and domain databases
InterPro	IPR000169. Pept_cys_AS. IPR004134. Peptidase_C1B. [Graphical view]
PANTHER	PTHR10363. Peptidase_C1B. 1 hit.
Pfam	PF03051. Peptidase_C1_2. 1 hit. [Graphical view]
PIRSF	PIRSF005700. PepC. 1 hit.
PROSITE	PS00640. THIOL_PROTEASE_ASN. False negative. PS00139. THIOL_PROTEASE_CYS. 1 hit. PS00639. THIOL_PROTEASE_HIS. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PEPC_LACDL

Accession

Primary (citable) accession number: Q48543

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)