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Q484R8

- LIPA_COLP3

UniProt

Q484R8 - LIPA_COLP3

Protein

Lipoyl synthase

Gene

lipA

Organism
Colwellia psychrerythraea (strain 34H / ATCC BAA-681) (Vibrio psychroerythus)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 62 (01 Oct 2014)
      Sequence version 1 (13 Sep 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

    Catalytic activityi

    Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi75 – 751Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi80 – 801Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi86 – 861Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi101 – 1011Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi105 – 1051Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi108 – 1081Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. lipoate synthase activity Source: UniProtKB-HAMAP
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. protein lipoylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciCPSY167879:GI48-1709-MONOMER.
    UniPathwayiUPA00538; UER00593.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lipoyl synthaseUniRule annotation (EC:2.8.1.8UniRule annotation)
    Alternative name(s):
    Lip-synUniRule annotation
    Short name:
    LSUniRule annotation
    Lipoate synthaseUniRule annotation
    Lipoic acid synthaseUniRule annotation
    Sulfur insertion protein LipAUniRule annotation
    Gene namesi
    Name:lipAUniRule annotation
    Ordered Locus Names:CPS_1709
    OrganismiColwellia psychrerythraea (strain 34H / ATCC BAA-681) (Vibrio psychroerythus)
    Taxonomic identifieri167879 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesColwelliaceaeColwellia
    ProteomesiUP000000547: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 328328Lipoyl synthasePRO_0000325240Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi167879.CPS_1709.

    Structurei

    3D structure databases

    ProteinModelPortaliQ484R8.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0320.
    HOGENOMiHOG000235997.
    KOiK03644.
    OMAiHPHIPTK.
    OrthoDBiEOG6038ZS.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_00206. Lipoyl_synth.
    InterProiIPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view]
    PANTHERiPTHR10949. PTHR10949. 1 hit.
    PfamiPF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
    SMARTiSM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00510. lipA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q484R8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSNDLTSESK TANRAAGEKF RDADKLAHIP IKVVSSTKAT MLRKPSWLRI    50
    KLPKSSERID NIKANLRKND LHSVCEEASC PNLSECFNHG TATFMILGDI 100
    CTRRCPFCDV GHGRPLAPKS DEPKKLANSL KDMGLKYVVI TSVDRDDLRD 150
    GGAQQFADCV KEIGEQAPNT KVEILVPDFR GRMDRALEIL NQNPPHVFNH 200
    NMETAPRLYT KVRPGANYQW SLDLLKRFGE ANPDVTTKSG LMVGLGETNE 250
    EILEVMQDLR DHGVTMLTVG QYLQPSKDHL AVERYVHPDD FAMFEREAKK 300
    MGYEHAACGP LVRSSYHADK QAAGEEVK 328
    Length:328
    Mass (Da):36,707
    Last modified:September 13, 2005 - v1
    Checksum:i320F57FC37FA4B91
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000083 Genomic DNA. Translation: AAZ28477.1.
    RefSeqiYP_268447.1. NC_003910.7.

    Genome annotation databases

    EnsemblBacteriaiAAZ28477; AAZ28477; CPS_1709.
    GeneIDi3522737.
    KEGGicps:CPS_1709.
    PATRICi21466589. VBIColPsy94388_1542.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000083 Genomic DNA. Translation: AAZ28477.1 .
    RefSeqi YP_268447.1. NC_003910.7.

    3D structure databases

    ProteinModelPortali Q484R8.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 167879.CPS_1709.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAZ28477 ; AAZ28477 ; CPS_1709 .
    GeneIDi 3522737.
    KEGGi cps:CPS_1709.
    PATRICi 21466589. VBIColPsy94388_1542.

    Phylogenomic databases

    eggNOGi COG0320.
    HOGENOMi HOG000235997.
    KOi K03644.
    OMAi HPHIPTK.
    OrthoDBi EOG6038ZS.

    Enzyme and pathway databases

    UniPathwayi UPA00538 ; UER00593 .
    BioCyci CPSY167879:GI48-1709-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_00206. Lipoyl_synth.
    InterProi IPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view ]
    PANTHERi PTHR10949. PTHR10949. 1 hit.
    Pfami PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
    SMARTi SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00510. lipA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 34H / ATCC BAA-681.

    Entry informationi

    Entry nameiLIPA_COLP3
    AccessioniPrimary (citable) accession number: Q484R8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 18, 2008
    Last sequence update: September 13, 2005
    Last modified: October 1, 2014
    This is version 62 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3