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Q484Q5 (SYL_COLP3) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:CPS_1722
OrganismColwellia psychrerythraea (strain 34H / ATCC BAA-681) (Vibrio psychroerythus) [Complete proteome] [HAMAP]
Taxonomic identifier167879 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesColwelliaceaeColwellia

Protein attributes

Sequence length863 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 863863Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000009332

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif618 – 6225"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6211ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q484Q5 [UniParc].

Last modified September 13, 2005. Version 1.
Checksum: 86F7EE2A5585161F

FASTA86397,091
        10         20         30         40         50         60 
MEAIYNPQAI EATVQKFWTD NNTFQAIENP DKEKFYCLAM FPYPSGRLHM GHVRNYSLGD 

        70         80         90        100        110        120 
VISRYQRMQG KNVMQPMGWD AFGLPAENAA IKNKTAPGKW TYENIDYMRN QLQSLGFGYD 

       130        140        150        160        170        180 
WGRELATCKP DYYRWEQWFF TQLFEKGLVY KKNATVNWDP VDQTVLANEQ VIDGRGWRSG 

       190        200        210        220        230        240 
ALVERKQIPQ WFIKITDYAE ELLDDLDQLT EWPEQVKTMQ RNWIGRSQGV EMTFAVADST 

       250        260        270        280        290        300 
ESFDIYTTRP DTLMGVTYVA LAAQHPLAVA AAVDNADLAA FIDECKNSKT TEADMAAMEK 

       310        320        330        340        350        360 
KGVDTGLKAI HPLTGKLVPV WAANFVLMDY GSGAVMSVPG HDQRDYEFAL KYGLAIEQVI 

       370        380        390        400        410        420 
AGQEADDINK AAITEKSTLI NSGEFDGLDF EEAFKAISDK LISENKGKTT TNYRLRDWGV 

       430        440        450        460        470        480 
SRQRYWGTPI PMINLANGES VPVPTNELPV VLPEDVVMNG TTSPIKADPE WAKTLYNGEE 

       490        500        510        520        530        540 
ALRETDTFDT FMESSWYYAR YCSPNDDTQM IDPAKANYWL PVDQYIGGIE HAILHLLYSR 

       550        560        570        580        590        600 
FFHKLLRDVG LVKCDEPFKK LLCQGMVLAE TYYREADNGA QEWIAPTDVE VERDEKGQIT 

       610        620        630        640        650        660 
SSISKIDGQP VLSAGMSKMS KSKNNGIDPQ EVIEKYGADT VRLFIMFTSP PEQTLEWSDA 

       670        680        690        700        710        720 
GVEGAHRFVK RVYKLAHEFV ESTNNSAVVD IAELTLNADH KKLRRELHKT IAKVTDDIGR 

       730        740        750        760        770        780 
RNTFNTAIAA IMELMNHLGK AKVNSDEDKA VMQEAVRAVV LMLTPITPHL CHHLWQLVGG 

       790        800        810        820        830        840 
SDENVEDASW PVVDNSALVE DEKLIIVQVN GKVRAKITVA ADASKEDVEA LGLNDESVLK 

       850        860 
FTDGNTIRKV IYIPGKLLNI VAN 

« Hide

References

[1]"The psychrophilic lifestyle as revealed by the genome sequence of Colwellia psychrerythraea 34H through genomic and proteomic analyses."
Methe B.A., Nelson K.E., Deming J.W., Momen B., Melamud E., Zhang X., Moult J., Madupu R., Nelson W.C., Dodson R.J., Brinkac L.M., Daugherty S.C., Durkin A.S., DeBoy R.T., Kolonay J.F., Sullivan S.A., Zhou L., Davidsen T.M. expand/collapse author list , Wu M., Huston A.L., Lewis M., Weaver B., Weidman J.F., Khouri H., Utterback T.R., Feldblyum T.V., Fraser C.M.
Proc. Natl. Acad. Sci. U.S.A. 102:10913-10918(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 34H / ATCC BAA-681.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000083 Genomic DNA. Translation: AAZ26868.1.
RefSeqYP_268460.1. NC_003910.7.

3D structure databases

ProteinModelPortalQ484Q5.
SMRQ484Q5. Positions 228-408.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING167879.CPS_1722.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAZ26868; AAZ26868; CPS_1722.
GeneID3521123.
KEGGcps:CPS_1722.
PATRIC21466613. VBIColPsy94388_1554.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycCPSY167879:GI48-1722-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_COLP3
AccessionPrimary (citable) accession number: Q484Q5
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: September 13, 2005
Last modified: April 16, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries