ID   GLF1_KLEPN              Reviewed;         384 AA.
AC   Q48485;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   16-JUN-2009, entry version 52.
DE   RecName: Full=Probable UDP-galactopyranose mutase;
DE            EC=5.4.99.9;
GN   Name=rfbD;
OS   Klebsiella pneumoniae.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Klebsiella.
OX   NCBI_TaxID=573;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=K20 / Serotype O1;
RX   MEDLINE=96032372; PubMed=7559323;
RA   Clarke B.R., Bronner D., Keenleyside W.J., Severn W.B., Richards J.C.,
RA   Whitfield C.;
RT   "Role of Rfe and RfbF in the initiation of biosynthesis of D-galactan
RT   I, the lipopolysaccharide O antigen from Klebsiella pneumoniae
RT   serotype O1.";
RL   J. Bacteriol. 177:5411-5418(1995).
CC   -!- FUNCTION: Involved in the conversion of UDP-GalP into UDP-GalF
CC       through a 2-keto intermediate.
CC   -!- CATALYTIC ACTIVITY: UDP-D-galactopyranose = UDP-D-galacto-1,4-
CC       furanose.
CC   -!- COFACTOR: FAD (By similarity).
CC   -!- PATHWAY: Bacterial outer membrane biogenesis; LPS O-antigen
CC       biosynthesis.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution-NoDerivs License
CC   -----------------------------------------------------------------------
DR   EMBL; L31762; AAC98417.1; -; Genomic_DNA.
DR   PDB; 1WAM; X-ray; 2.35 A; A=1-384.
DR   PDB; 2BI7; X-ray; 2.00 A; A=1-384.
DR   PDB; 2BI8; X-ray; 2.35 A; A=1-384.
DR   PDBsum; 1WAM; -.
DR   PDBsum; 2BI7; -.
DR   PDBsum; 2BI8; -.
DR   BRENDA; 5.4.99.9; 758.
DR   GO; GO:0005488; F:binding; IEA:InterPro.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:InterPro.
DR   GO; GO:0008767; F:UDP-galactopyranose mutase activity; IEA:EC.
DR   GO; GO:0009103; P:lipopolysaccharide biosynthetic process; IEA:UniProtKB-KW.
DR   InterPro; IPR006076; FAD-dep_OxRdtase.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   InterPro; IPR004379; UDP-GALP_mutase.
DR   InterPro; IPR015899; UDP-GalPyranose_mutase_C.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   Pfam; PF01266; DAO; 1.
DR   Pfam; PF03275; GLF; 1.
DR   TIGRFAMs; TIGR00031; UDP-GALP_mutase; 1.
PE   1: Evidence at protein level;
KW   3D-structure; FAD; Flavoprotein; Isomerase;
KW   Lipopolysaccharide biosynthesis.
FT   CHAIN         1    384       Probable UDP-galactopyranose mutase.
FT                                /FTId=PRO_0000087506.
FT   NP_BIND       5     33       FAD (Potential).
FT   STRAND        5      9
FT   HELIX        13     24
FT   STRAND       28     32
FT   STRAND       34     39
FT   HELIX        40     42
FT   STRAND       44     46
FT   STRAND       48     50
FT   STRAND       53     55
FT   STRAND       62     65
FT   HELIX        67     74
FT   STRAND       79     81
FT   STRAND       86     90
FT   STRAND       93     98
FT   HELIX       101    107
FT   HELIX       114    122
FT   HELIX       145    151
FT   HELIX       153    160
FT   STRAND      166    169
FT   STRAND      178    181
FT   STRAND      189    194
FT   HELIX       197    205
FT   STRAND      211    214
FT   HELIX       221    226
FT   STRAND      227    232
FT   HELIX       236    239
FT   TURN        240    244
FT   STRAND      249    261
FT   STRAND      263    271
FT   STRAND      276    283
FT   HELIX       284    287
FT   STRAND      294    305
FT   HELIX       319    332
FT   STRAND      336    340
FT   HELIX       342    345
FT   HELIX       352    371
SQ   SEQUENCE   384 AA;  44457 MW;  B74EC65EA5B751AB CRC64;
     MKSKKILIVG AGFSGAVIGR QLAEKGHQVH IIDQRDHIGG NSYDARDSET NVMVHVYGPH
     IFHTDNETVW NYVNKHAEMM PYVNRVKATV NGQVFSLPIN LHTINQFFSK TCSPDEARAL
     IAEKGDSTIA DPQTFEEQAL RFIGKELYEA FFKGYTIKQW GMQPSELPAS ILKRLPVRFN
     YDDNYFNHKF QGMPKCGYTQ MIKSILNHEN IKVDLQREFI VEERTHYDHV FYSGPLDAFY
     GYQYGRLGYR TLDFKKFTYQ GDYQGCAVMN YCSVDVPYTR ITEHKYFSPW EQHDGSVCYK
     EYSRACEEND IPYYPIRQMG EMALLEKYLS LAENETNITF VGRLGTYRYL DMDVTIAEAL
     KTAEVYLNSL TENQPMPVFT VSVR
//