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Q48485

- GLF1_KLEPN

UniProt

Q48485 - GLF1_KLEPN

Protein

UDP-galactopyranose mutase

Gene

rfbD

Organism
Klebsiella pneumoniae
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 79 (01 Oct 2014)
      Sequence version 1 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Involved in the biosynthesis of the galactose-containing O-side-chain polysaccharide backbone structure of D-galactan I which is a key component of lipopolysaccharide (LPS). Catalyzes the interconversion through a 2-keto intermediate of uridine diphosphogalactopyranose (UDP-GalP) into uridine diphosphogalactofuranose (UDP-GalF) which is the biosynthetic precursor of galactofuranosyl residues.1 Publication

    Catalytic activityi

    UDP-alpha-D-galactopyranose = UDP-alpha-D-galactofuranose.1 Publication

    Cofactori

    Binds 1 FAD per subunit.5 Publications

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei14 – 141FAD4 Publications
    Binding sitei41 – 411FAD; via amide nitrogen4 Publications
    Binding sitei84 – 841UDP-GalP
    Binding sitei151 – 1511UDP-GalP; via carbonyl oxygen
    Binding sitei156 – 1561UDP-GalP
    Binding sitei160 – 1601UDP-GalP
    Binding sitei185 – 1851UDP-GalP
    Binding sitei219 – 2191FAD; via amide nitrogen4 Publications
    Binding sitei270 – 2701UDP-GalP
    Binding sitei280 – 2801UDP-GalP
    Binding sitei314 – 3141UDP-GalP
    Binding sitei343 – 3431FAD4 Publications
    Binding sitei349 – 3491UDP-GalP

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi33 – 342FAD4 Publications
    Nucleotide bindingi60 – 612FAD4 Publications
    Nucleotide bindingi350 – 3556FAD4 Publications

    GO - Molecular functioni

    1. UDP-galactopyranose mutase activity Source: UniProtKB-EC

    GO - Biological processi

    1. O antigen biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Isomerase

    Keywords - Biological processi

    Lipopolysaccharide biosynthesis

    Keywords - Ligandi

    FAD, Flavoprotein

    Enzyme and pathway databases

    UniPathwayiUPA00281.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    UDP-galactopyranose mutase (EC:5.4.99.9)
    Short name:
    UGM
    Alternative name(s):
    UDP-GALP mutase
    Uridine 5-diphosphate galactopyranose mutase
    Gene namesi
    Name:rfbD
    OrganismiKlebsiella pneumoniae
    Taxonomic identifieri573 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeKlebsiella

    Pathology & Biotechi

    Disruption phenotypei

    Abolishes lipopolysaccharide (LPS) biosynthesis.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2323Sequence AnalysisAdd
    BLAST
    Chaini24 – 384361UDP-galactopyranose mutasePRO_0000087506Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.5 Publications

    Structurei

    Secondary structure

    1
    384
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi5 – 95
    Helixi13 – 2311
    Turni24 – 263
    Beta strandi28 – 3912
    Helixi40 – 423
    Beta strandi44 – 463
    Turni48 – 503
    Beta strandi53 – 553
    Beta strandi62 – 654
    Helixi67 – 748
    Beta strandi79 – 813
    Beta strandi86 – 905
    Beta strandi93 – 986
    Helixi101 – 1077
    Helixi114 – 12411
    Helixi135 – 1439
    Helixi145 – 1517
    Helixi153 – 1608
    Helixi164 – 1663
    Helixi169 – 1713
    Beta strandi179 – 1813
    Beta strandi189 – 1946
    Helixi197 – 20610
    Beta strandi211 – 2166
    Helixi221 – 2266
    Beta strandi227 – 2326
    Helixi236 – 2394
    Turni240 – 2445
    Beta strandi249 – 26113
    Beta strandi263 – 2719
    Beta strandi276 – 2838
    Helixi284 – 2874
    Beta strandi294 – 30512
    Helixi319 – 33214
    Beta strandi336 – 3405
    Helixi342 – 3454
    Helixi352 – 37120

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1WAMX-ray2.35A1-384[»]
    2BI7X-ray2.00A1-384[»]
    2BI8X-ray2.35A1-384[»]
    3GF4X-ray2.45A/B1-384[»]
    3INRX-ray2.30A/B1-383[»]
    3INTX-ray2.51A/B1-384[»]
    3KYBX-ray2.30A/B1-384[»]
    ProteinModelPortaliQ48485.
    SMRiQ48485. Positions 2-384.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ48485.

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di3.40.50.720. 3 hits.
    InterProiIPR016040. NAD(P)-bd_dom.
    IPR004379. UDP-GALP_mutase.
    IPR015899. UDP-GalPyranose_mutase_C.
    [Graphical view]
    PANTHERiPTHR21197. PTHR21197. 1 hit.
    PfamiPF03275. GLF. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00031. UDP-GALP_mutase. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q48485-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKSKKILIVG AGFSGAVIGR QLAEKGHQVH IIDQRDHIGG NSYDARDSET    50
    NVMVHVYGPH IFHTDNETVW NYVNKHAEMM PYVNRVKATV NGQVFSLPIN 100
    LHTINQFFSK TCSPDEARAL IAEKGDSTIA DPQTFEEQAL RFIGKELYEA 150
    FFKGYTIKQW GMQPSELPAS ILKRLPVRFN YDDNYFNHKF QGMPKCGYTQ 200
    MIKSILNHEN IKVDLQREFI VEERTHYDHV FYSGPLDAFY GYQYGRLGYR 250
    TLDFKKFTYQ GDYQGCAVMN YCSVDVPYTR ITEHKYFSPW EQHDGSVCYK 300
    EYSRACEEND IPYYPIRQMG EMALLEKYLS LAENETNITF VGRLGTYRYL 350
    DMDVTIAEAL KTAEVYLNSL TENQPMPVFT VSVR 384
    Length:384
    Mass (Da):44,457
    Last modified:November 1, 1997 - v1
    Checksum:iB74EC65EA5B751AB
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L31762 Genomic DNA. Translation: AAC98417.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L31762 Genomic DNA. Translation: AAC98417.1 .

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1WAM X-ray 2.35 A 1-384 [» ]
    2BI7 X-ray 2.00 A 1-384 [» ]
    2BI8 X-ray 2.35 A 1-384 [» ]
    3GF4 X-ray 2.45 A/B 1-384 [» ]
    3INR X-ray 2.30 A/B 1-383 [» ]
    3INT X-ray 2.51 A/B 1-384 [» ]
    3KYB X-ray 2.30 A/B 1-384 [» ]
    ProteinModelPortali Q48485.
    SMRi Q48485. Positions 2-384.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00281 .

    Miscellaneous databases

    EvolutionaryTracei Q48485.

    Family and domain databases

    Gene3Di 3.40.50.720. 3 hits.
    InterProi IPR016040. NAD(P)-bd_dom.
    IPR004379. UDP-GALP_mutase.
    IPR015899. UDP-GalPyranose_mutase_C.
    [Graphical view ]
    PANTHERi PTHR21197. PTHR21197. 1 hit.
    Pfami PF03275. GLF. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00031. UDP-GALP_mutase. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Role of Rfe and RfbF in the initiation of biosynthesis of D-galactan I, the lipopolysaccharide O antigen from Klebsiella pneumoniae serotype O1."
      Clarke B.R., Bronner D., Keenleyside W.J., Severn W.B., Richards J.C., Whitfield C.
      J. Bacteriol. 177:5411-5418(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K20 / Serotype O1.
    2. "UDP-galactofuranose precursor required for formation of the lipopolysaccharide O antigen of Klebsiella pneumoniae serotype O1 is synthesized by the product of the rfbDKPO1 gene."
      Koplin R., Brisson J.R., Whitfield C.
      J. Biol. Chem. 272:4121-4128(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, DISRUPTION PHENOTYPE, COFACTOR, SUBUNIT.
      Strain: K20 / Serotype O1.
    3. Cited for: REACTION MECHANISM.
      Strain: K20 / Serotype O1.
    4. "Crystal structures of Mycobacteria tuberculosis and Klebsiella pneumoniae UDP-galactopyranose mutase in the oxidised state and Klebsiella pneumoniae UDP-galactopyranose mutase in the (active) reduced state."
      Beis K., Srikannathasan V., Liu H., Fullerton S.W., Bamford V.A., Sanders D.A., Whitfield C., McNeil M.R., Naismith J.H.
      J. Mol. Biol. 348:971-982(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.35 ANGSTROMS) IN COMPLEX WITH FAD, COFACTOR, SUBUNIT.
    5. "X-ray crystallography reveals a reduced substrate complex of UDP-galactopyranose mutase poised for covalent catalysis by flavin."
      Gruber T.D., Westler W.M., Kiessling L.L., Forest K.T.
      Biochemistry 48:9171-9173(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 1-383 IN COMPLEX WITH FAD AND SUBSTRATE, COFACTOR, SUBUNIT.
    6. "Ligand binding and substrate discrimination by UDP-galactopyranose mutase."
      Gruber T.D., Borrok M.J., Westler W.M., Forest K.T., Kiessling L.L.
      J. Mol. Biol. 391:327-340(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.45 ANGSTROMS) OF 1-383 IN COMPLEX WITH FAD AND SUBSTRATE, COFACTOR.
    7. "Structure of UDP-galactopyranose mutase bound to flavin mononucleotide."
      Gruber T.D., Dimond M.C., Kiessling L.L., Forest K.T.
      Submitted (DEC-2009) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (2.30 ANGSTROMS) OF 1-383 IN COMPLEX WITH FAD, COFACTOR, SUBUNIT.

    Entry informationi

    Entry nameiGLF1_KLEPN
    AccessioniPrimary (citable) accession number: Q48485
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 79 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3