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Q48485

- GLF1_KLEPN

UniProt

Q48485 - GLF1_KLEPN

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Protein
UDP-galactopyranose mutase
Gene
rfbD
Organism
Klebsiella pneumoniae
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in the biosynthesis of the galactose-containing O-side-chain polysaccharide backbone structure of D-galactan I which is a key component of lipopolysaccharide (LPS). Catalyzes the interconversion through a 2-keto intermediate of uridine diphosphogalactopyranose (UDP-GalP) into uridine diphosphogalactofuranose (UDP-GalF) which is the biosynthetic precursor of galactofuranosyl residues.1 Publication

Catalytic activityi

UDP-alpha-D-galactopyranose = UDP-alpha-D-galactofuranose.1 Publication

Cofactori

Binds 1 FAD per subunit.5 Publications

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei14 – 141FAD
Binding sitei41 – 411FAD; via amide nitrogen
Binding sitei84 – 841UDP-GalP
Binding sitei151 – 1511UDP-GalP; via carbonyl oxygen
Binding sitei156 – 1561UDP-GalP
Binding sitei160 – 1601UDP-GalP
Binding sitei185 – 1851UDP-GalP
Binding sitei219 – 2191FAD; via amide nitrogen
Binding sitei270 – 2701UDP-GalP
Binding sitei280 – 2801UDP-GalP
Binding sitei314 – 3141UDP-GalP
Binding sitei343 – 3431FAD
Binding sitei349 – 3491UDP-GalP

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi33 – 342FAD
Nucleotide bindingi60 – 612FAD
Nucleotide bindingi350 – 3556FAD

GO - Molecular functioni

  1. UDP-galactopyranose mutase activity Source: UniProtKB-EC

GO - Biological processi

  1. O antigen biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Lipopolysaccharide biosynthesis

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

UniPathwayiUPA00281.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-galactopyranose mutase (EC:5.4.99.9)
Short name:
UGM
Alternative name(s):
UDP-GALP mutase
Uridine 5-diphosphate galactopyranose mutase
Gene namesi
Name:rfbD
OrganismiKlebsiella pneumoniae
Taxonomic identifieri573 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeKlebsiella

Pathology & Biotechi

Disruption phenotypei

Abolishes lipopolysaccharide (LPS) biosynthesis.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323 Reviewed prediction
Add
BLAST
Chaini24 – 384361UDP-galactopyranose mutase
PRO_0000087506Add
BLAST

Interactioni

Subunit structurei

Homodimer.4 Publications

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi5 – 95
Helixi13 – 2311
Turni24 – 263
Beta strandi28 – 3912
Helixi40 – 423
Beta strandi44 – 463
Turni48 – 503
Beta strandi53 – 553
Beta strandi62 – 654
Helixi67 – 748
Beta strandi79 – 813
Beta strandi86 – 905
Beta strandi93 – 986
Helixi101 – 1077
Helixi114 – 12411
Helixi135 – 1439
Helixi145 – 1517
Helixi153 – 1608
Helixi164 – 1663
Helixi169 – 1713
Beta strandi179 – 1813
Beta strandi189 – 1946
Helixi197 – 20610
Beta strandi211 – 2166
Helixi221 – 2266
Beta strandi227 – 2326
Helixi236 – 2394
Turni240 – 2445
Beta strandi249 – 26113
Beta strandi263 – 2719
Beta strandi276 – 2838
Helixi284 – 2874
Beta strandi294 – 30512
Helixi319 – 33214
Beta strandi336 – 3405
Helixi342 – 3454
Helixi352 – 37120

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1WAMX-ray2.35A1-384[»]
2BI7X-ray2.00A1-384[»]
2BI8X-ray2.35A1-384[»]
3GF4X-ray2.45A/B1-384[»]
3INRX-ray2.30A/B1-383[»]
3INTX-ray2.51A/B1-384[»]
3KYBX-ray2.30A/B1-384[»]
ProteinModelPortaliQ48485.
SMRiQ48485. Positions 2-384.

Miscellaneous databases

EvolutionaryTraceiQ48485.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.40.50.720. 3 hits.
InterProiIPR016040. NAD(P)-bd_dom.
IPR004379. UDP-GALP_mutase.
IPR015899. UDP-GalPyranose_mutase_C.
[Graphical view]
PANTHERiPTHR21197. PTHR21197. 1 hit.
PfamiPF03275. GLF. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00031. UDP-GALP_mutase. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q48485-1 [UniParc]FASTAAdd to Basket

« Hide

MKSKKILIVG AGFSGAVIGR QLAEKGHQVH IIDQRDHIGG NSYDARDSET    50
NVMVHVYGPH IFHTDNETVW NYVNKHAEMM PYVNRVKATV NGQVFSLPIN 100
LHTINQFFSK TCSPDEARAL IAEKGDSTIA DPQTFEEQAL RFIGKELYEA 150
FFKGYTIKQW GMQPSELPAS ILKRLPVRFN YDDNYFNHKF QGMPKCGYTQ 200
MIKSILNHEN IKVDLQREFI VEERTHYDHV FYSGPLDAFY GYQYGRLGYR 250
TLDFKKFTYQ GDYQGCAVMN YCSVDVPYTR ITEHKYFSPW EQHDGSVCYK 300
EYSRACEEND IPYYPIRQMG EMALLEKYLS LAENETNITF VGRLGTYRYL 350
DMDVTIAEAL KTAEVYLNSL TENQPMPVFT VSVR 384
Length:384
Mass (Da):44,457
Last modified:November 1, 1997 - v1
Checksum:iB74EC65EA5B751AB
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L31762 Genomic DNA. Translation: AAC98417.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L31762 Genomic DNA. Translation: AAC98417.1 .

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1WAM X-ray 2.35 A 1-384 [» ]
2BI7 X-ray 2.00 A 1-384 [» ]
2BI8 X-ray 2.35 A 1-384 [» ]
3GF4 X-ray 2.45 A/B 1-384 [» ]
3INR X-ray 2.30 A/B 1-383 [» ]
3INT X-ray 2.51 A/B 1-384 [» ]
3KYB X-ray 2.30 A/B 1-384 [» ]
ProteinModelPortali Q48485.
SMRi Q48485. Positions 2-384.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00281 .

Miscellaneous databases

EvolutionaryTracei Q48485.

Family and domain databases

Gene3Di 3.40.50.720. 3 hits.
InterProi IPR016040. NAD(P)-bd_dom.
IPR004379. UDP-GALP_mutase.
IPR015899. UDP-GalPyranose_mutase_C.
[Graphical view ]
PANTHERi PTHR21197. PTHR21197. 1 hit.
Pfami PF03275. GLF. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00031. UDP-GALP_mutase. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Role of Rfe and RfbF in the initiation of biosynthesis of D-galactan I, the lipopolysaccharide O antigen from Klebsiella pneumoniae serotype O1."
    Clarke B.R., Bronner D., Keenleyside W.J., Severn W.B., Richards J.C., Whitfield C.
    J. Bacteriol. 177:5411-5418(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K20 / Serotype O1.
  2. "UDP-galactofuranose precursor required for formation of the lipopolysaccharide O antigen of Klebsiella pneumoniae serotype O1 is synthesized by the product of the rfbDKPO1 gene."
    Koplin R., Brisson J.R., Whitfield C.
    J. Biol. Chem. 272:4121-4128(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, DISRUPTION PHENOTYPE, COFACTOR, SUBUNIT.
    Strain: K20 / Serotype O1.
  3. Cited for: REACTION MECHANISM.
    Strain: K20 / Serotype O1.
  4. "Crystal structures of Mycobacteria tuberculosis and Klebsiella pneumoniae UDP-galactopyranose mutase in the oxidised state and Klebsiella pneumoniae UDP-galactopyranose mutase in the (active) reduced state."
    Beis K., Srikannathasan V., Liu H., Fullerton S.W., Bamford V.A., Sanders D.A., Whitfield C., McNeil M.R., Naismith J.H.
    J. Mol. Biol. 348:971-982(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.35 ANGSTROMS) IN COMPLEX WITH FAD, COFACTOR, SUBUNIT.
  5. "X-ray crystallography reveals a reduced substrate complex of UDP-galactopyranose mutase poised for covalent catalysis by flavin."
    Gruber T.D., Westler W.M., Kiessling L.L., Forest K.T.
    Biochemistry 48:9171-9173(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 1-383 IN COMPLEX WITH FAD AND SUBSTRATE, COFACTOR, SUBUNIT.
  6. "Ligand binding and substrate discrimination by UDP-galactopyranose mutase."
    Gruber T.D., Borrok M.J., Westler W.M., Forest K.T., Kiessling L.L.
    J. Mol. Biol. 391:327-340(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.45 ANGSTROMS) OF 1-383 IN COMPLEX WITH FAD AND SUBSTRATE, COFACTOR.
  7. "Structure of UDP-galactopyranose mutase bound to flavin mononucleotide."
    Gruber T.D., Dimond M.C., Kiessling L.L., Forest K.T.
    Submitted (DEC-2009) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.30 ANGSTROMS) OF 1-383 IN COMPLEX WITH FAD, COFACTOR, SUBUNIT.

Entry informationi

Entry nameiGLF1_KLEPN
AccessioniPrimary (citable) accession number: Q48485
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: July 9, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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