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Protein

UDP-galactopyranose mutase

Gene

rfbD

Organism
Klebsiella pneumoniae
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the biosynthesis of the galactose-containing O-side-chain polysaccharide backbone structure of D-galactan I which is a key component of lipopolysaccharide (LPS). Catalyzes the interconversion through a 2-keto intermediate of uridine diphosphogalactopyranose (UDP-GalP) into uridine diphosphogalactofuranose (UDP-GalF) which is the biosynthetic precursor of galactofuranosyl residues.1 Publication

Catalytic activityi

UDP-alpha-D-galactopyranose = UDP-alpha-D-galactofuranose.1 Publication

Cofactori

FAD5 PublicationsNote: Binds 1 FAD per subunit.5 Publications

Pathwayi: LPS O-antigen biosynthesis

This protein is involved in the pathway LPS O-antigen biosynthesis, which is part of Bacterial outer membrane biogenesis.
View all proteins of this organism that are known to be involved in the pathway LPS O-antigen biosynthesis and in Bacterial outer membrane biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei14FAD4 Publications1
Binding sitei41FAD; via amide nitrogen4 Publications1
Binding sitei84UDP-GalP1
Binding sitei151UDP-GalP; via carbonyl oxygen1
Binding sitei156UDP-GalP1
Binding sitei160UDP-GalP1
Binding sitei185UDP-GalP1
Binding sitei219FAD; via amide nitrogen4 Publications1
Binding sitei270UDP-GalP1
Binding sitei280UDP-GalP1
Binding sitei314UDP-GalP1
Binding sitei343FAD4 Publications1
Binding sitei349UDP-GalP1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi33 – 34FAD4 Publications2
Nucleotide bindingi60 – 61FAD4 Publications2
Nucleotide bindingi350 – 355FAD4 Publications6

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Lipopolysaccharide biosynthesis

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

BRENDAi5.4.99.9. 2814.
UniPathwayiUPA00281.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-galactopyranose mutase (EC:5.4.99.9)
Short name:
UGM
Alternative name(s):
UDP-GALP mutase
Uridine 5-diphosphate galactopyranose mutase
Gene namesi
Name:rfbD
OrganismiKlebsiella pneumoniae
Taxonomic identifieri573 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeKlebsiella

Pathology & Biotechi

Disruption phenotypei

Abolishes lipopolysaccharide (LPS) biosynthesis.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
ChainiPRO_000008750624 – 384UDP-galactopyranose mutaseAdd BLAST361

Interactioni

Subunit structurei

Homodimer.5 Publications

Structurei

Secondary structure

1384
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi5 – 9Combined sources5
Helixi13 – 23Combined sources11
Turni24 – 26Combined sources3
Beta strandi28 – 39Combined sources12
Helixi40 – 42Combined sources3
Beta strandi44 – 46Combined sources3
Turni48 – 50Combined sources3
Beta strandi53 – 55Combined sources3
Beta strandi62 – 65Combined sources4
Helixi67 – 74Combined sources8
Beta strandi79 – 81Combined sources3
Beta strandi86 – 90Combined sources5
Beta strandi93 – 98Combined sources6
Helixi101 – 107Combined sources7
Helixi114 – 124Combined sources11
Helixi135 – 143Combined sources9
Helixi145 – 151Combined sources7
Helixi153 – 160Combined sources8
Helixi164 – 166Combined sources3
Helixi169 – 171Combined sources3
Beta strandi179 – 181Combined sources3
Beta strandi189 – 194Combined sources6
Helixi197 – 206Combined sources10
Beta strandi211 – 216Combined sources6
Helixi221 – 226Combined sources6
Beta strandi227 – 232Combined sources6
Helixi236 – 239Combined sources4
Turni240 – 244Combined sources5
Beta strandi249 – 261Combined sources13
Beta strandi263 – 271Combined sources9
Beta strandi276 – 283Combined sources8
Helixi284 – 287Combined sources4
Beta strandi294 – 305Combined sources12
Helixi319 – 332Combined sources14
Beta strandi336 – 340Combined sources5
Helixi342 – 345Combined sources4
Helixi352 – 371Combined sources20

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WAMX-ray2.35A1-384[»]
2BI7X-ray2.00A1-384[»]
2BI8X-ray2.35A1-384[»]
3GF4X-ray2.45A/B1-384[»]
3INRX-ray2.30A/B1-383[»]
3INTX-ray2.51A/B1-384[»]
3KYBX-ray2.30A/B1-384[»]
ProteinModelPortaliQ48485.
SMRiQ48485.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ48485.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.40.50.720. 3 hits.
InterProiIPR016040. NAD(P)-bd_dom.
IPR004379. UDP-GALP_mutase.
IPR015899. UDP-GalPyranose_mutase_C.
[Graphical view]
PANTHERiPTHR21197. PTHR21197. 1 hit.
PfamiPF03275. GLF. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00031. UDP-GALP_mutase. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q48485-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSKKILIVG AGFSGAVIGR QLAEKGHQVH IIDQRDHIGG NSYDARDSET
60 70 80 90 100
NVMVHVYGPH IFHTDNETVW NYVNKHAEMM PYVNRVKATV NGQVFSLPIN
110 120 130 140 150
LHTINQFFSK TCSPDEARAL IAEKGDSTIA DPQTFEEQAL RFIGKELYEA
160 170 180 190 200
FFKGYTIKQW GMQPSELPAS ILKRLPVRFN YDDNYFNHKF QGMPKCGYTQ
210 220 230 240 250
MIKSILNHEN IKVDLQREFI VEERTHYDHV FYSGPLDAFY GYQYGRLGYR
260 270 280 290 300
TLDFKKFTYQ GDYQGCAVMN YCSVDVPYTR ITEHKYFSPW EQHDGSVCYK
310 320 330 340 350
EYSRACEEND IPYYPIRQMG EMALLEKYLS LAENETNITF VGRLGTYRYL
360 370 380
DMDVTIAEAL KTAEVYLNSL TENQPMPVFT VSVR
Length:384
Mass (Da):44,457
Last modified:November 1, 1997 - v1
Checksum:iB74EC65EA5B751AB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L31762 Genomic DNA. Translation: AAC98417.1.
RefSeqiWP_023284018.1. NZ_MAMU01000050.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L31762 Genomic DNA. Translation: AAC98417.1.
RefSeqiWP_023284018.1. NZ_MAMU01000050.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WAMX-ray2.35A1-384[»]
2BI7X-ray2.00A1-384[»]
2BI8X-ray2.35A1-384[»]
3GF4X-ray2.45A/B1-384[»]
3INRX-ray2.30A/B1-383[»]
3INTX-ray2.51A/B1-384[»]
3KYBX-ray2.30A/B1-384[»]
ProteinModelPortaliQ48485.
SMRiQ48485.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00281.
BRENDAi5.4.99.9. 2814.

Miscellaneous databases

EvolutionaryTraceiQ48485.

Family and domain databases

Gene3Di3.40.50.720. 3 hits.
InterProiIPR016040. NAD(P)-bd_dom.
IPR004379. UDP-GALP_mutase.
IPR015899. UDP-GalPyranose_mutase_C.
[Graphical view]
PANTHERiPTHR21197. PTHR21197. 1 hit.
PfamiPF03275. GLF. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00031. UDP-GALP_mutase. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGLF1_KLEPN
AccessioniPrimary (citable) accession number: Q48485
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.