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Q48481 (GLF8_KLEPN) Reviewed, UniProtKB/Swiss-Prot

Last modified January 22, 2014. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable UDP-galactopyranose mutase

Short name=UGM
EC=5.4.99.9
Alternative name(s):
UDP-GALP mutase
Uridine 5-diphosphate galactopyranose mutase
Gene names
Name:rfbD
OrganismKlebsiella pneumoniae
Taxonomic identifier573 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeKlebsiella

Protein attributes

Sequence length384 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the interconversion through a 2-keto intermediate of uridine diphosphogalactopyranose (UDP-GalP) into uridine diphosphogalactofuranose (UDP-GalF) By similarity.

Catalytic activity

UDP-alpha-D-galactopyranose = UDP-alpha-D-galactofuranose.

Cofactor

Binds 1 FAD per subunit By similarity.

Pathway

Bacterial outer membrane biogenesis; LPS O-antigen biosynthesis.

Subunit structure

Homodimer By similarity.

Sequence similarities

Belongs to the UDP-galactopyranose/dTDP-fucopyranose mutase family.

Ontologies

Keywords
   Biological processLipopolysaccharide biosynthesis
   DomainSignal
   LigandFAD
Flavoprotein
   Molecular functionIsomerase
Gene Ontology (GO)
   Biological_processO antigen biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_functionUDP-galactopyranose mutase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919 Potential
Chain20 – 384365Probable UDP-galactopyranose mutase
PRO_0000087507

Regions

Nucleotide binding33 – 342FAD By similarity
Nucleotide binding60 – 612FAD By similarity
Nucleotide binding350 – 3556FAD By similarity

Sites

Binding site141FAD By similarity
Binding site411FAD; via amide nitrogen By similarity
Binding site841UDP-GalP By similarity
Binding site1511UDP-GalP; via carbonyl oxygen By similarity
Binding site1561UDP-GalP By similarity
Binding site1601UDP-GalP By similarity
Binding site1851UDP-GalP By similarity
Binding site2191FAD; via amide nitrogen By similarity
Binding site2701UDP-GalP By similarity
Binding site2801UDP-GalP By similarity
Binding site3141UDP-GalP By similarity
Binding site3431FAD By similarity
Binding site3491UDP-GalP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q48481 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: AA812676E6D060FF

FASTA38444,475
        10         20         30         40         50         60 
MNNKNIMIVG AGFSGVVIAR QLAEQGYTVK IIDRRDHIGG NSYDTRDPQT DVMVHVYGPH 

        70         80         90        100        110        120 
IFHTDNETVW NYVNQYAEMM PYVNRVKATV NGQVFSLPIN LHTINQFFAK TCSPDEARAL 

       130        140        150        160        170        180 
ISEKGDSSIV EPQTFEEQAL RFIGKELYEA FFKGYTIKQW GMEPSELPAS ILKRLPVRFN 

       190        200        210        220        230        240 
YDDNYFNHKF QGMPKLGYTR MIEAIADHEN ISIELQREFL PEEREDYAHV FYSGPLDAFY 

       250        260        270        280        290        300 
SYQYGRLGYR TLDFEKFTYQ GDYQGCAVMN YCSIDVPYTR ITEHKYFSPW ESHEGSVCYK 

       310        320        330        340        350        360 
EYSRACGEND IPYYPIRQMG EMALLEKYLS LAESEKNITF VGRLGTYRYL DMDVTIAEAL 

       370        380 
KTADEFLSSV ANQEEMPVFT VPVR 

« Hide

References

[1]"Clonally diverse rfb gene clusters are involved in expression of a family of related D-galactan O antigens in Klebsiella species."
Kelly R.F., Whitfield C.
J. Bacteriol. 178:5205-5214(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Serotype O8.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L41518 Genomic DNA. Translation: AAC98408.1.

3D structure databases

ProteinModelPortalQ48481.
SMRQ48481. Positions 3-384.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00281.

Family and domain databases

Gene3D3.40.50.720. 3 hits.
InterProIPR016040. NAD(P)-bd_dom.
IPR004379. UDP-GALP_mutase.
IPR015899. UDP-GalPyranose_mutase_C.
[Graphical view]
PANTHERPTHR21197. PTHR21197. 1 hit.
PfamPF03275. GLF. 1 hit.
[Graphical view]
TIGRFAMsTIGR00031. UDP-GALP_mutase. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLF8_KLEPN
AccessionPrimary (citable) accession number: Q48481
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: January 22, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways