Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Probable UDP-galactopyranose mutase

Gene

rfbD

Organism
Klebsiella pneumoniae
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the interconversion through a 2-keto intermediate of uridine diphosphogalactopyranose (UDP-GalP) into uridine diphosphogalactofuranose (UDP-GalF).By similarity

Catalytic activityi

UDP-alpha-D-galactopyranose = UDP-alpha-D-galactofuranose.

Cofactori

FADBy similarityNote: Binds 1 FAD per subunit.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei14 – 141FADBy similarity
Binding sitei41 – 411FAD; via amide nitrogenBy similarity
Binding sitei84 – 841UDP-GalPBy similarity
Binding sitei151 – 1511UDP-GalP; via carbonyl oxygenBy similarity
Binding sitei156 – 1561UDP-GalPBy similarity
Binding sitei160 – 1601UDP-GalPBy similarity
Binding sitei185 – 1851UDP-GalPBy similarity
Binding sitei219 – 2191FAD; via amide nitrogenBy similarity
Binding sitei270 – 2701UDP-GalPBy similarity
Binding sitei280 – 2801UDP-GalPBy similarity
Binding sitei314 – 3141UDP-GalPBy similarity
Binding sitei343 – 3431FADBy similarity
Binding sitei349 – 3491UDP-GalPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi33 – 342FADBy similarity
Nucleotide bindingi60 – 612FADBy similarity
Nucleotide bindingi350 – 3556FADBy similarity

GO - Molecular functioni

  1. UDP-galactopyranose mutase activity Source: UniProtKB-EC

GO - Biological processi

  1. O antigen biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Lipopolysaccharide biosynthesis

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

UniPathwayiUPA00281.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable UDP-galactopyranose mutase (EC:5.4.99.9)
Short name:
UGM
Alternative name(s):
UDP-GALP mutase
Uridine 5-diphosphate galactopyranose mutase
Gene namesi
Name:rfbD
OrganismiKlebsiella pneumoniae
Taxonomic identifieri573 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeKlebsiella

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Sequence AnalysisAdd
BLAST
Chaini20 – 384365Probable UDP-galactopyranose mutasePRO_0000087507Add
BLAST

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ48481.
SMRiQ48481. Positions 3-384.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.40.50.720. 3 hits.
InterProiIPR016040. NAD(P)-bd_dom.
IPR004379. UDP-GALP_mutase.
IPR015899. UDP-GalPyranose_mutase_C.
[Graphical view]
PANTHERiPTHR21197. PTHR21197. 1 hit.
PfamiPF03275. GLF. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00031. UDP-GALP_mutase. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q48481-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNNKNIMIVG AGFSGVVIAR QLAEQGYTVK IIDRRDHIGG NSYDTRDPQT
60 70 80 90 100
DVMVHVYGPH IFHTDNETVW NYVNQYAEMM PYVNRVKATV NGQVFSLPIN
110 120 130 140 150
LHTINQFFAK TCSPDEARAL ISEKGDSSIV EPQTFEEQAL RFIGKELYEA
160 170 180 190 200
FFKGYTIKQW GMEPSELPAS ILKRLPVRFN YDDNYFNHKF QGMPKLGYTR
210 220 230 240 250
MIEAIADHEN ISIELQREFL PEEREDYAHV FYSGPLDAFY SYQYGRLGYR
260 270 280 290 300
TLDFEKFTYQ GDYQGCAVMN YCSIDVPYTR ITEHKYFSPW ESHEGSVCYK
310 320 330 340 350
EYSRACGEND IPYYPIRQMG EMALLEKYLS LAESEKNITF VGRLGTYRYL
360 370 380
DMDVTIAEAL KTADEFLSSV ANQEEMPVFT VPVR
Length:384
Mass (Da):44,475
Last modified:November 1, 1997 - v1
Checksum:iAA812676E6D060FF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L41518 Genomic DNA. Translation: AAC98408.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L41518 Genomic DNA. Translation: AAC98408.1.

3D structure databases

ProteinModelPortaliQ48481.
SMRiQ48481. Positions 3-384.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00281.

Family and domain databases

Gene3Di3.40.50.720. 3 hits.
InterProiIPR016040. NAD(P)-bd_dom.
IPR004379. UDP-GALP_mutase.
IPR015899. UDP-GalPyranose_mutase_C.
[Graphical view]
PANTHERiPTHR21197. PTHR21197. 1 hit.
PfamiPF03275. GLF. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00031. UDP-GALP_mutase. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Clonally diverse rfb gene clusters are involved in expression of a family of related D-galactan O antigens in Klebsiella species."
    Kelly R.F., Whitfield C.
    J. Bacteriol. 178:5205-5214(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Serotype O8.

Entry informationi

Entry nameiGLF8_KLEPN
AccessioniPrimary (citable) accession number: Q48481
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 26, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.