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Protein

Probable UDP-galactopyranose mutase

Gene

rfbD

Organism
Klebsiella pneumoniae
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the interconversion through a 2-keto intermediate of uridine diphosphogalactopyranose (UDP-GalP) into uridine diphosphogalactofuranose (UDP-GalF).By similarity

Catalytic activityi

UDP-alpha-D-galactopyranose = UDP-alpha-D-galactofuranose.

Cofactori

FADBy similarityNote: Binds 1 FAD per subunit.By similarity

Pathwayi: LPS O-antigen biosynthesis

This protein is involved in the pathway LPS O-antigen biosynthesis, which is part of Bacterial outer membrane biogenesis.
View all proteins of this organism that are known to be involved in the pathway LPS O-antigen biosynthesis and in Bacterial outer membrane biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei14FADBy similarity1
Binding sitei41FAD; via amide nitrogenBy similarity1
Binding sitei84UDP-GalPBy similarity1
Binding sitei151UDP-GalP; via carbonyl oxygenBy similarity1
Binding sitei156UDP-GalPBy similarity1
Binding sitei160UDP-GalPBy similarity1
Binding sitei185UDP-GalPBy similarity1
Binding sitei219FAD; via amide nitrogenBy similarity1
Binding sitei270UDP-GalPBy similarity1
Binding sitei280UDP-GalPBy similarity1
Binding sitei314UDP-GalPBy similarity1
Binding sitei343FADBy similarity1
Binding sitei349UDP-GalPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi33 – 34FADBy similarity2
Nucleotide bindingi60 – 61FADBy similarity2
Nucleotide bindingi350 – 355FADBy similarity6

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Lipopolysaccharide biosynthesis

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

UniPathwayiUPA00281.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable UDP-galactopyranose mutase (EC:5.4.99.9)
Short name:
UGM
Alternative name(s):
UDP-GALP mutase
Uridine 5-diphosphate galactopyranose mutase
Gene namesi
Name:rfbD
OrganismiKlebsiella pneumoniae
Taxonomic identifieri573 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeKlebsiella

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
ChainiPRO_000008750720 – 384Probable UDP-galactopyranose mutaseAdd BLAST365

Interactioni

Subunit structurei

Homodimer.By similarity

Chemistry databases

BindingDBiQ48481.

Structurei

3D structure databases

ProteinModelPortaliQ48481.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.40.50.720. 3 hits.
InterProiIPR016040. NAD(P)-bd_dom.
IPR004379. UDP-GALP_mutase.
IPR015899. UDP-GalPyranose_mutase_C.
[Graphical view]
PANTHERiPTHR21197. PTHR21197. 1 hit.
PfamiPF03275. GLF. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00031. UDP-GALP_mutase. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q48481-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNNKNIMIVG AGFSGVVIAR QLAEQGYTVK IIDRRDHIGG NSYDTRDPQT
60 70 80 90 100
DVMVHVYGPH IFHTDNETVW NYVNQYAEMM PYVNRVKATV NGQVFSLPIN
110 120 130 140 150
LHTINQFFAK TCSPDEARAL ISEKGDSSIV EPQTFEEQAL RFIGKELYEA
160 170 180 190 200
FFKGYTIKQW GMEPSELPAS ILKRLPVRFN YDDNYFNHKF QGMPKLGYTR
210 220 230 240 250
MIEAIADHEN ISIELQREFL PEEREDYAHV FYSGPLDAFY SYQYGRLGYR
260 270 280 290 300
TLDFEKFTYQ GDYQGCAVMN YCSIDVPYTR ITEHKYFSPW ESHEGSVCYK
310 320 330 340 350
EYSRACGEND IPYYPIRQMG EMALLEKYLS LAESEKNITF VGRLGTYRYL
360 370 380
DMDVTIAEAL KTADEFLSSV ANQEEMPVFT VPVR
Length:384
Mass (Da):44,475
Last modified:November 1, 1997 - v1
Checksum:iAA812676E6D060FF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L41518 Genomic DNA. Translation: AAC98408.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L41518 Genomic DNA. Translation: AAC98408.1.

3D structure databases

ProteinModelPortaliQ48481.
ModBaseiSearch...
MobiDBiSearch...

Chemistry databases

BindingDBiQ48481.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00281.

Family and domain databases

Gene3Di3.40.50.720. 3 hits.
InterProiIPR016040. NAD(P)-bd_dom.
IPR004379. UDP-GALP_mutase.
IPR015899. UDP-GalPyranose_mutase_C.
[Graphical view]
PANTHERiPTHR21197. PTHR21197. 1 hit.
PfamiPF03275. GLF. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00031. UDP-GALP_mutase. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGLF8_KLEPN
AccessioniPrimary (citable) accession number: Q48481
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.