Q48406 (BLAT_KLEOX) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-lactamase TEM-12
EC=3.5.2.6

Gene names

Name:

blaT-12b

Encoded on

Plasmid pOZ201 Ref.1

Organism

Klebsiella oxytoca

Taxonomic identifier

571 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Klebsiella

Protein attributes

Sequence length	286 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	TEM-type are the most prevalent beta-lactamases in enterobacteria; they hydrolyze the beta-lactam bond in susceptible beta-lactam antibiotics, thus conferring resistance to penicillins and cephalosporins such as ceftazidime. Ref.1
Catalytic activity	A beta-lactam + H2O = a substituted beta-amino acid.
Sequence similarities	Belongs to the class-A beta-lactamase family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Domain	Signal
Molecular function	Hydrolase
PTM	Disulfide bond
Technical term	Plasmid
Gene Ontology (GO)
Biological_process	beta-lactam antibiotic catabolic process Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	beta-lactamase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 286	263	Beta-lactamase TEM-12		PRO_0000364051
Regions
	Region	232 – 234	3	Substrate binding By similarity
Sites
	Active site	68	1	Acyl-ester intermediate By similarity
	Active site	166	1	Proton acceptor By similarity
Amino acid modifications
	Disulfide bond	75 ↔ 121		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q48406 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: BB6788F3BBA8924B	FASTA	286	31,446
	10 20 30 40 50 60 MSIQHFRVAL IPFFAAFCLP VFAHPETLVK VKDAEDQLGA RVGYIELDLN SGKILESFRP 70 80 90 100 110 120 EERFPMMSTF KVLLCGAVLS RVDAGQEQLG RRIHYSQNDL VEYSPVTEKH LTDGMTVREL 130 140 150 160 170 180 CSAAITMSDN TAANLLLTTI GGPKELTAFL HNMGDHVTRL DSWEPELNEA IPNDERDTTM 190 200 210 220 230 240 PAAMATTLRK LLTGELLTLA SRQQLIDWME ADKVAGPLLR SALPAGWFIA DKSGAGERGS 250 260 270 280 RGIIAALGPD GKPSRIVVIY TTGSQATMDE RNRQIAEIGA SLIKHW

References

[1]

"Transposition of the gene encoding a TEM-12 extended-spectrum beta-lactamase." Heritage J., Hawkey P.M., Todd N., Lewis I.J. Antimicrob. Agents Chemother. 36:1981-1986(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION IN RESISTANCE TO CEFTAZIDIME. Strain: 0400-1. Transposon: Tn841.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M88143 Genomic DNA. Translation: AAA25053.1.
3D structure databases
HSSP	HSSP built from PDB template 1XPB based on UniProtKB P62593.
ProteinModelPortal	Q48406.
SMR	Q48406. Positions 24-286.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.40.710.10. 1 hit.
InterPro	IPR012338. Beta-lactam/transpept-like. IPR000871. Beta-lactam_class-A/D. IPR023650. Beta-lactam_class-A_AS. [Graphical view]
PRINTS	PR00118. BLACTAMASEA.
SUPFAM	SSF56601. PBP_transp_fold. 1 hit.
PROSITE	PS00146. BETA_LACTAMASE_A. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BLAT_KLEOX

Accession

Primary (citable) accession number: Q48406

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 10, 2009
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program