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Q48406

- BLAT_KLEOX

UniProt

Q48406 - BLAT_KLEOX

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Protein
Beta-lactamase TEM-12
Gene
blaT-12b
Organism
Klebsiella oxytoca
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

TEM-type are the most prevalent beta-lactamases in enterobacteria; they hydrolyze the beta-lactam bond in susceptible beta-lactam antibiotics, thus conferring resistance to penicillins and cephalosporins such as ceftazidime.1 Publication

Catalytic activityi

A beta-lactam + H2O = a substituted beta-amino acid.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei68 – 681Acyl-ester intermediate By similarity
Active sitei166 – 1661Proton acceptor By similarity

GO - Molecular functioni

  1. beta-lactamase activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. beta-lactam antibiotic catabolic process Source: InterPro
  2. response to antibiotic Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Antibiotic resistance

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-lactamase TEM-12 (EC:3.5.2.6)
Gene namesi
Name:blaT-12b
Encoded oniPlasmid pOZ2011 Publication
OrganismiKlebsiella oxytoca
Taxonomic identifieri571 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeKlebsiella

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323 Reviewed prediction
Add
BLAST
Chaini24 – 286263Beta-lactamase TEM-12
PRO_0000364051Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi75 ↔ 121 By similarity

Keywords - PTMi

Disulfide bond

Structurei

3D structure databases

ProteinModelPortaliQ48406.
SMRiQ48406. Positions 24-286.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni232 – 2343Substrate binding By similarity

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A/D.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]
PRINTSiPR00118. BLACTAMASEA.
SUPFAMiSSF56601. SSF56601. 1 hit.
PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q48406-1 [UniParc]FASTAAdd to Basket

« Hide

MSIQHFRVAL IPFFAAFCLP VFAHPETLVK VKDAEDQLGA RVGYIELDLN    50
SGKILESFRP EERFPMMSTF KVLLCGAVLS RVDAGQEQLG RRIHYSQNDL 100
VEYSPVTEKH LTDGMTVREL CSAAITMSDN TAANLLLTTI GGPKELTAFL 150
HNMGDHVTRL DSWEPELNEA IPNDERDTTM PAAMATTLRK LLTGELLTLA 200
SRQQLIDWME ADKVAGPLLR SALPAGWFIA DKSGAGERGS RGIIAALGPD 250
GKPSRIVVIY TTGSQATMDE RNRQIAEIGA SLIKHW 286
Length:286
Mass (Da):31,446
Last modified:November 1, 1996 - v1
Checksum:iBB6788F3BBA8924B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M88143 Genomic DNA. Translation: AAA25053.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M88143 Genomic DNA. Translation: AAA25053.1 .

3D structure databases

ProteinModelPortali Q48406.
SMRi Q48406. Positions 24-286.
ModBasei Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.710.10. 1 hit.
InterProi IPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A/D.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view ]
PRINTSi PR00118. BLACTAMASEA.
SUPFAMi SSF56601. SSF56601. 1 hit.
PROSITEi PS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Transposition of the gene encoding a TEM-12 extended-spectrum beta-lactamase."
    Heritage J., Hawkey P.M., Todd N., Lewis I.J.
    Antimicrob. Agents Chemother. 36:1981-1986(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION IN RESISTANCE TO CEFTAZIDIME.
    Strain: 0400-1.
    Transposon: Tn841.
    Plasmid: pOZ201

Entry informationi

Entry nameiBLAT_KLEOX
AccessioniPrimary (citable) accession number: Q48406
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: November 1, 1996
Last modified: July 9, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Plasmid

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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