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Q48406 (BLAT_KLEOX) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-lactamase TEM-12

EC=3.5.2.6
Gene names
Name:blaT-12b
Encoded onPlasmid pOZ201 Ref.1
OrganismKlebsiella oxytoca
Taxonomic identifier571 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeKlebsiella

Protein attributes

Sequence length286 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

TEM-type are the most prevalent beta-lactamases in enterobacteria; they hydrolyze the beta-lactam bond in susceptible beta-lactam antibiotics, thus conferring resistance to penicillins and cephalosporins such as ceftazidime. Ref.1

Catalytic activity

A beta-lactam + H2O = a substituted beta-amino acid.

Sequence similarities

Belongs to the class-A beta-lactamase family.

Ontologies

Keywords
   Biological processAntibiotic resistance
   DomainSignal
   Molecular functionHydrolase
   PTMDisulfide bond
   Technical termPlasmid
Gene Ontology (GO)
   Biological_processbeta-lactam antibiotic catabolic process

Inferred from electronic annotation. Source: InterPro

response to antibiotic

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionbeta-lactamase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 Potential
Chain24 – 286263Beta-lactamase TEM-12
PRO_0000364051

Regions

Region232 – 2343Substrate binding By similarity

Sites

Active site681Acyl-ester intermediate By similarity
Active site1661Proton acceptor By similarity

Amino acid modifications

Disulfide bond75 ↔ 121 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q48406 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: BB6788F3BBA8924B

FASTA28631,446
        10         20         30         40         50         60 
MSIQHFRVAL IPFFAAFCLP VFAHPETLVK VKDAEDQLGA RVGYIELDLN SGKILESFRP 

        70         80         90        100        110        120 
EERFPMMSTF KVLLCGAVLS RVDAGQEQLG RRIHYSQNDL VEYSPVTEKH LTDGMTVREL 

       130        140        150        160        170        180 
CSAAITMSDN TAANLLLTTI GGPKELTAFL HNMGDHVTRL DSWEPELNEA IPNDERDTTM 

       190        200        210        220        230        240 
PAAMATTLRK LLTGELLTLA SRQQLIDWME ADKVAGPLLR SALPAGWFIA DKSGAGERGS 

       250        260        270        280 
RGIIAALGPD GKPSRIVVIY TTGSQATMDE RNRQIAEIGA SLIKHW 

« Hide

References

[1]"Transposition of the gene encoding a TEM-12 extended-spectrum beta-lactamase."
Heritage J., Hawkey P.M., Todd N., Lewis I.J.
Antimicrob. Agents Chemother. 36:1981-1986(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION IN RESISTANCE TO CEFTAZIDIME.
Strain: 0400-1.
Transposon: Tn841.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M88143 Genomic DNA. Translation: AAA25053.1.

3D structure databases

ProteinModelPortalQ48406.
SMRQ48406. Positions 24-286.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.40.710.10. 1 hit.
InterProIPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A/D.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]
PRINTSPR00118. BLACTAMASEA.
SUPFAMSSF56601. SSF56601. 1 hit.
PROSITEPS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBLAT_KLEOX
AccessionPrimary (citable) accession number: Q48406
Entry history
Integrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: November 1, 1996
Last modified: July 9, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families