Reviewed, UniProtKB/Swiss-Prot Q48335 (G3P_HALVA)

Names and origin

Protein names

Recommended name:
    Glyceraldehyde-3-phosphate dehydrogenase
    EC=1.2.1.59
Alternative name(s):
    NAD(P)-dependent glyceraldehyde-3-phosphate dehydrogenase
      Short name=GAPDH

Gene names

Name:

gap

Organism

Haloarcula vallismortis

Taxonomic identifier

28442 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Haloarcula

Protein attributes

Sequence length	335 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate + phosphate + NAD(P)(+) = 3-phospho-D-glyceroyl phosphate + NAD(P)H.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
Subunit structure	Homotetramer By similarity.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	NAD NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro glyceraldehyde-3-phosphate dehydrogenase (NAD(P)+) (phosphorylating) activity Inferred from electronic annotation. Source: EC glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 335	335	Glyceraldehyde-3-phosphate dehydrogenase		PRO_0000145739
Regions
	Nucleotide binding	15 – 16	2	NAD By similarity
	Region	155 – 157	3	Glyceraldehyde 3-phosphate binding By similarity
	Region	214 – 215	2	Glyceraldehyde 3-phosphate binding By similarity
Sites
	Active site	156	1	Nucleophile By similarity
	Binding site	37	1	NAD By similarity
	Binding site	186	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	201	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	237	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	301	1	NAD; via carbonyl oxygen By similarity
	Binding site	318	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q48335-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: F25BF308255E03BB	FASTA	335	35,941
	10 20 30 40 50 60 MMSEPVRVGL NGFGRIGRNV FRASLHSDDV EIVGINDVMD DSEIDYFAQY DSVMGELEGA 70 80 90 100 110 120 SVDDGVLTVD GTDFEAGIFH ETDPTQLPWD DLDVDVAFEA TGIFRTKEDA SQHLDAGADK 130 140 150 160 170 180 VLISAPPKGD EPVKQLVYGV NHDEYDGEDV VSNASCTTNS ITPVAKVLDE EFGINAGQLT 190 200 210 220 230 240 TVHAYTGSQN LMDGPNGKPR RRRAAAENII PTSTGAAQAA TEVLPELEGK LDGMAIRVPV 250 260 270 280 290 300 PNGSITEFVV DLDDDVTESD VNAAFEDAAA GELEGVLGVT SDDVVSSDIL GDPYSTQVDL 310 320 330 QSTNVVSGMT KILTWYDNEY GFSNRMLDVA EYITE

References

[1]

"Higher-plant chloroplast and cytosolic 3-phosphoglycerate kinases: a case of endosymbiotic gene replacement." Brinkmann H., Martin W. Plant Mol. Biol. 30:65-75(1996) [PubMed: 8616244] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 29715 / DSM 3756 / IFO 14741 / JCM 8877 / NCMB 2082.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L47295 Genomic DNA. Translation: AAB03730.1.
PIR	S65043.
3D structure databases
HSSP	HSSP built from PDB template 1NQO based on UniProtKB P00362.
ModBase	Search...
Family and domain databases
InterPro	IPR000173. GlycerAld_3-P_DHase. IPR006424. Glyceraldehyde-3-P_DHase_1. [Graphical view]
PANTHER	PTHR10836. GAP_DH. 1 hit.
Pfam	PF02800. Gp_dh_C. 1 hit. PF00044. Gp_dh_N. 1 hit. [Graphical view]
PIRSF	PIRSF000149. GAP_DH. 1 hit.
PRINTS	PR00078. G3PDHDRGNASE.
TIGRFAMs	TIGR01534. GAPDH-I. 1 hit.
PROSITE	PS00071. GAPDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G3P_HALVA

Accession

Primary (citable) accession number: Q48335

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	November 25, 2008
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)