Reviewed, UniProtKB/Swiss-Prot Q48303 (DHDM_HYPSX)

Names and origin

Protein names

Recommended name:
    Dimethylamine dehydrogenase
      Short name=DMADh
    EC=1.5.8.1

Gene names

Name:

dmd

Organism

Hyphomicrobium sp. (strain x)

Taxonomic identifier

79673 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Hyphomicrobiaceae › Hyphomicrobium

Protein attributes

Sequence length	736 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Dimethylamine + H(2)O + electron-transferring flavoprotein = methylamine + formaldehyde + reduced electron-transferring flavoprotein.
Cofactor	Binds 1 FMN covalently per subunit By similarity. Binds 1 4Fe-4S cluster per subunit By similarity.
Sequence similarities	In the N-terminal section; belongs to the NADH:flavin oxidoreductase/NADH oxidase family.

Ontologies

Keywords
Ligand	4Fe-4S FMN Flavoprotein Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW FMN binding Inferred from electronic annotation. Source: InterPro dimethylamine dehydrogenase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 736	735	Dimethylamine dehydrogenase		PRO_0000194471
Regions
	Nucleotide binding	398 – 427	30	ADP By similarity
	Region	176 – 179	4	Substrate-binding By similarity
Sites
	Active site	181	1	Proton donor By similarity
	Metal binding	352	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	355	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	358	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	371	1	Iron-sulfur (4Fe-4S) By similarity
	Binding site	229	1	FMN By similarity
	Binding site	329	1	FMN By similarity
Amino acid modifications
	Modified residue	31	1	S-6-FMN cysteine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q48303-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: A3EE943F5617851B	FASTA	736	82,655
	10 20 30 40 50 60 MARDPRFDIL FTPLKLGSKT IRNRFYQVPH CNGAGTNSPG MNMAHRGIKA EGGWGAVNTE 70 80 90 100 110 120 QCSIHPECDD TLRITARIWD QGDMRNLRAM VDHVHSHGSL AGCELFYGGP HAPAIESRTI 130 140 150 160 170 180 SRGPSQYNSE FATVPGCPGF TYNHEADIDE LERLQQQYVD AALRARDTGF DLVNVYGAHA 190 200 210 220 230 240 YGPMQWLNPY YNRRTDKYGG SFDNRARFWI ETLEKIRRAV NDDVALVTRC ATDTLYGTKG 250 260 270 280 290 300 VELTEDGLRF IELASPYLDL WDVNIGDIAE WGEDAGPSRF YPIAHENDWI RHIKQATNKP 310 320 330 340 350 360 VVGVGRYYDP EKMLQVIKAG IIDIIGAARP SIADPWLPRK IDEGRVDDIR TCIGCNVCIS 370 380 390 400 410 420 RWEMGGVPFI CTQNATAGEE YRRGWHPEKF EPKKSDHDVL IVGAGPAGSE CARVLMERGY 430 440 450 460 470 480 TVHLVDTREK TGGYVNDVAT LPGLGEWSFH RDYRQTQLEK LLKKNPECQI ALKQKPMTAD 490 500 510 520 530 540 DILQYGASRV VIATGAKWST TGVNHRTHEP IPGADASLPH VLTPEQVYEG KKAVGKRVMI 550 560 570 580 590 600 INYDAYYTAP SLAEKFARAG HDVTVATVCG LGAYMEYTLE GANMQRLIHE LGIKVLGETG 610 620 630 640 650 660 CSRVEQGRVE LFNIWGEGYK RSYKGAGQLP RNENTSHEWH ECDTVILVTS RRSEDTLYRE 670 680 690 700 710 720 LKARKGEWEA NGITNVFVIG DAESPRIIAD ATFDGHRLAR EIEDADPQHQ KPYKREQRAW 730 GTAYNPDENP DLVWRV

References

[1]

"The primary structure of Hyphomicrobium X dimethylamine dehydrogenase. Relationship to trimethylamine dehydrogenase and implications for substrate recognition." Yang C.C., Packman L.C., Scrutton N.S. Eur. J. Biochem. 232:264-271(1995) [PubMed: 7556160] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	X89575 Genomic DNA. Translation: CAA61752.1.
3D structure databases
HSSP	HSSP built from PDB template 1O94 based on UniProtKB P16099.
ModBase	Search...
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR001155. OxRdtase_FMN_N. IPR001100. Pyr_nuc-diS_OxRdtase. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF00724. Oxidored_FMN. 1 hit. PF07992. Pyr_redox_2. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR. PR00411. PNDRDTASEI.
ProtoNet	Search...

Entry information

Entry name

DHDM_HYPSX

Accession

Primary (citable) accession number: Q48303

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 23, 2007
Last modified:	December 16, 2008
This is version 56 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)