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Q482P5 (RLMI_COLP3) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 50. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Ribosomal RNA large subunit methyltransferase I
EC=2.1.1.191
Alternative name(s):
23S rRNA m5C1962 methyltransferase
rRNA (cytosine-C(5)-)-methyltransferase RlmI
Gene names
Name:rlmI
Ordered Locus Names:CPS_2247
OrganismColwellia psychrerythraea (strain 34H / ATCC BAA-681) (Vibrio psychroerythus) [Complete proteome] [HAMAP]
Taxonomic identifier167879 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Colwelliaceae › Colwellia ›

Protein attributes

Sequence length396 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA By similarity. HAMAP-Rule MF_01857

Catalytic activity

S-adenosyl-L-methionine + cytosine(1962) in 23S rRNA = S-adenosyl-L-homocysteine + 5-methylcytosine(1962) in 23S rRNA. HAMAP-Rule MF_01857

Subcellular location

Cytoplasm Potential HAMAP-Rule MF_01857.

Sequence similarities

Belongs to the methyltransferase superfamily. RlmI family.

Contains 1 PUA domain.

Ontologies

Keywords
   Biological processrRNA processing
   Cellular componentCytoplasm
   LigandRNA-binding
S-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionRNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

rRNA (cytosine) methyltransferase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 396396Ribosomal RNA large subunit methyltransferase I HAMAP-Rule MF_01857
PRO_0000366219

Regions

Domain2 – 7978PUA

Sequences

Sequence LengthMass (Da)Tools
Q482P5 [UniParc].

Last modified September 13, 2005. Version 1.
Checksum: 5ED389965472A3E4

FASTA39644,582
        10         20         30         40         50         60 
MSARISLNVG REKSLLRKHP WIFSKAVNKI KGNPMLGDTV DVFDSKGNWL AKGAYSPESQ 

        70         80         90        100        110        120 
IRIRVWSFDE HEEIDRDFFF AKLQSAQARR DWFISQGKLT GYRLIAGESD GLPGVTIDKY 

       130        140        150        160        170        180 
DNFIVCQLLS AGADFHRYTI VNCLTELYPG CHIYERSDVD VRKKEGLEPV TGWLTEPQDS 

       190        200        210        220        230        240 
TECIIEEHGI KIHVDIATGH KTGFYLDQRD SRLAAGKFAK DKTVLNCFSY TSTFALHCAA 

       250        260        270        280        290        300 
NNAKEVINVD VSQDALDMGE RNLALNGLSD ANVSFVKEDV FKLLRRYREE KRTFDMIILD 

       310        320        330        340        350        360 
PPKFVESKAQ LTGACRGYKD INMIAMQLLN PGGLLLTFSC SGLMEASLFQ KVVADAALDA 

       370        380        390 
KRKAYFVERL QQAADHPISS NYPEGYYLKG LVCQVE

« Hide

References

[1]"The psychrophilic lifestyle as revealed by the genome sequence of Colwellia psychrerythraea 34H through genomic and proteomic analyses."
Methe B.A., Nelson K.E., Deming J.W., Momen B., Melamud E., Zhang X., Moult J., Madupu R., Nelson W.C., Dodson R.J., Brinkac L.M., Daugherty S.C., Durkin A.S., DeBoy R.T., Kolonay J.F., Sullivan S.A., Zhou L., Davidsen T.M. expand/collapse author list , Wu M., Huston A.L., Lewis M., Weaver B., Weidman J.F., Khouri H., Utterback T.R., Feldblyum T.V., Fraser C.M.
Proc. Natl. Acad. Sci. U.S.A. 102:10913-10918(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 34H / ATCC BAA-681.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000083 Genomic DNA. Translation: AAZ28133.1.
RefSeqYP_268972.1. NC_003910.7.

3D structure databases

HSSPHSSP built from PDB template 2CWW based on UniProtKB Q5SIT4.
ProteinModelPortalQ482P5.
ModBaseSearch...

Protein-protein interaction databases

STRING167879.CPS_2247.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAZ28133; AAZ28133; CPS_2247.
GeneID3522392.
KEGGcps:CPS_2247.
PATRIC21467597. VBIColPsy94388_2039.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1092.
HOGENOMHOG000266009.
KOK06969.
OMACSGLMTT.

Enzyme and pathway databases

BioCycCPSY167879:GI48-2317-MONOMER.

Family and domain databases

HAMAPMF_01857. 23SrRNA_methyltr_I.
InterProIPR002478. PUA.
IPR015947. PUA-like_domain.
IPR023542. rRNA_lsu_MeTfrase_I.
IPR019614. SAM-dep_methyl-trfase.
[Graphical view]
PfamPF10672. Methyltrans_SAM. 1 hit.
[Graphical view]
SMARTSM00359. PUA. 1 hit.
[Graphical view]
SUPFAMSSF88697. PUA-like. 1 hit.
PROSITEPS50890. PUA. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRLMI_COLP3
AccessionPrimary (citable) accession number: Q482P5
Entry history
Integrated into UniProtKB/Swiss-Prot: March 3, 2009
Last sequence update: September 13, 2005
Last modified: May 1, 2013
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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