Reviewed, UniProtKB/Swiss-Prot Q482M9 (RLMF_COLP3)

Names and origin

Protein names

Recommended name:
    Ribosomal RNA large subunit methyltransferase F
    EC=2.1.1.48
Alternative name(s):
    23S rRNA mA1618 methyltransferase
    rRNA adenine N-6-methyltransferase

Gene names

Name:	rlmF
Ordered Locus Names:	CPS_2268

Organism

Colwellia psychrerythraea (strain 34H / ATCC BAA-681) (Vibrio psychroerythus) [Complete proteome] [HAMAP]

Taxonomic identifier

167879 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Colwelliaceae › Colwellia

Protein attributes

Sequence length	301 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Specifically methylates the adenine in position 1618 of 23S rRNA By similarity.
Catalytic activity	S-adenosyl-L-methionine + rRNA = S-adenosyl-L-homocysteine + rRNA containing N(6)-methyladenine. HAMAP MF_01848
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the methyltransferase superfamily. METT10D/rlmF family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	rRNA modification Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	rRNA (adenine-N6-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 301	301	Ribosomal RNA large subunit methyltransferase F HAMAP MF_01848		PRO_0000349899

Sequences

	Sequence		Length	Mass (Da)
	Q482M9-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: A6A8292FC3745B7F	FASTA	301	34,029
	10 20 30 40 50 60 MHKKNRHNQG YNFTALVKAH PGLLQFIIKN QYNNQDTIDF ANPQAVKALN LSLLKSEYHV 70 80 90 100 110 120 KFWDIPDGYL CPAIPGRVDY IHHLQDLLAA TPKTLLPNKT PINVLDIGTG ASCIYPILGQ 130 140 150 160 170 180 REYDWHFVAS DVDPISIKVA KHIISSDKSL NRNINCRLQP NSNQIFNGII AEDEFYHLTI 190 200 210 220 230 240 CNPPFHSSLA EASKGTARKI KNLNKGNHSS KNQDKTLNFG GQKAELWCPG GELAFIGKMI 250 260 270 280 290 300 KESKAYQKQV LWFTCLVSKK DHLSKLKLSL KKSDAKQIKV IDMAQGQKIS RFIAWSFYDV N

References

[1]

"The psychrophilic lifestyle as revealed by the genome sequence of Colwellia psychrerythraea 34H through genomic and proteomic analyses." Methe B.A., Nelson K.E., Deming J.W., Momen B., Melamud E., Zhang X., Moult J., Madupu R., Nelson W.C., Dodson R.J., Brinkac L.M., Daugherty S.C., Durkin A.S., DeBoy R.T., Kolonay J.F., Sullivan S.A., Zhou L., Davidsen T.M. , Wu M., Huston A.L., Lewis M., Weaver B., Weidman J.F., Khouri H., Utterback T.R., Feldblyum T.V., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 102:10913-10918(2005) [PubMed: 16043709] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000083 Genomic DNA. Translation: AAZ28550.1.
RefSeq	YP_268988.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3522810.
GenomeReviews	Gene locus CPS_2268 in contig CP000083_GR.
KEGG	cps:CPS_2268.
NMPDR	fig|167879.3.peg.2205.
TIGR	CPS_2268.
Phylogenomic databases
HOGENOM	Q482M9.
OMA	Q482M9. LNFGGQN.
Family and domain databases
HAMAP	MF_01848. [Tree]
InterPro	IPR016909. S-AdoMet-dep_MeTrfase_YbiN_prd. IPR010286. SAM-Mtase_prd. [Graphical view]
PANTHER	PTHR13393. DUF890. 1 hit.
Pfam	PF05971. Methyltransf_10. 1 hit. [Graphical view]
PIRSF	PIRSF029038. Mtase_YbiN_prd. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RLMF_COLP3

Accession

Primary (citable) accession number: Q482M9

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	September 13, 2005
Last modified:	June 16, 2009
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)