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Q482M9 (RLMF_COLP3) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 36. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Ribosomal RNA large subunit methyltransferase F

EC=2.1.1.181
Alternative name(s):
23S rRNA mA1618 methyltransferase
rRNA adenine N-6-methyltransferase
Gene names
Name:rlmF
Ordered Locus Names:CPS_2268
OrganismColwellia psychrerythraea (strain 34H / ATCC BAA-681) (Vibrio psychroerythus) [Complete proteome] [HAMAP]
Taxonomic identifier167879 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesColwelliaceaeColwellia

Protein attributes

Sequence length301 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Specifically methylates the adenine in position 1618 of 23S rRNA By similarity. HAMAP MF_01848

Catalytic activity

S-adenosyl-L-methionine + adenine(1618) in 23S rRNA = S-adenosyl-L-homocysteine + rRNA containing N(6)-methyladenine(1618) in 23S rRNA. HAMAP MF_01848

Subcellular location

Cytoplasm Potential HAMAP MF_01848.

Sequence similarities

Belongs to the methyltransferase superfamily. METTL16/rlmF family.

Ontologies

Keywords
   Biological processrRNA processing
   Cellular componentCytoplasm
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionrRNA (adenine-N6-)-methyltransferase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 301301Ribosomal RNA large subunit methyltransferase F HAMAP MF_01848
PRO_0000349899

Sequences

Sequence LengthMass (Da)Tools
Q482M9 [UniParc].

Last modified September 13, 2005. Version 1.
Checksum: A6A8292FC3745B7F

FASTA30134,029
        10         20         30         40         50         60 
MHKKNRHNQG YNFTALVKAH PGLLQFIIKN QYNNQDTIDF ANPQAVKALN LSLLKSEYHV 

        70         80         90        100        110        120 
KFWDIPDGYL CPAIPGRVDY IHHLQDLLAA TPKTLLPNKT PINVLDIGTG ASCIYPILGQ 

       130        140        150        160        170        180 
REYDWHFVAS DVDPISIKVA KHIISSDKSL NRNINCRLQP NSNQIFNGII AEDEFYHLTI 

       190        200        210        220        230        240 
CNPPFHSSLA EASKGTARKI KNLNKGNHSS KNQDKTLNFG GQKAELWCPG GELAFIGKMI 

       250        260        270        280        290        300 
KESKAYQKQV LWFTCLVSKK DHLSKLKLSL KKSDAKQIKV IDMAQGQKIS RFIAWSFYDV 


N 

« Hide

References

[1]"The psychrophilic lifestyle as revealed by the genome sequence of Colwellia psychrerythraea 34H through genomic and proteomic analyses."
Methe B.A., Nelson K.E., Deming J.W., Momen B., Melamud E., Zhang X., Moult J., Madupu R., Nelson W.C., Dodson R.J., Brinkac L.M., Daugherty S.C., Durkin A.S., DeBoy R.T., Kolonay J.F., Sullivan S.A., Zhou L., Davidsen T.M. expand/collapse author list , Wu M., Huston A.L., Lewis M., Weaver B., Weidman J.F., Khouri H., Utterback T.R., Feldblyum T.V., Fraser C.M.
Proc. Natl. Acad. Sci. U.S.A. 102:10913-10918(2005) [PubMed: 16043709] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 34H / ATCC BAA-681.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000083 Genomic DNA. Translation: AAZ28550.1.
RefSeqYP_268988.1. NC_003910.7.

3D structure databases

ProteinModelPortalQ482M9.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ482M9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3522810.
GenomeReviewsGene locus CPS_2268 in contig CP000083_GR.
KEGGcps:CPS_2268.
NMPDRfig|167879.3.peg.2205.
PATRIC21467639. VBIColPsy94388_2055.
TIGRCPS_2268.

Phylogenomic databases

eggNOGCOG3129.
HOGENOMHBG747880.
OMADFANPEA.
ProtClustDBPRK11727.

Enzyme and pathway databases

BioCycCPSY167879:CPS_2268-MONOMER.

Family and domain databases

HAMAPMF_01848. 23SrRNA_methyltr_F.
[Tree]
InterProIPR016909. rRNA_lsu_methyltransferase_F.
IPR010286. SAM-Mtase_prd.
[Graphical view]
KOK06970.
PANTHERPTHR13393. DUF890. 1 hit.
PfamPF05971. Methyltransf_10. 1 hit.
[Graphical view]
PIRSFPIRSF029038. Mtase_YbiN_prd. 1 hit.
ProtoNetSearch...

Entry information

Entry nameRLMF_COLP3
AccessionPrimary (citable) accession number: Q482M9
Entry history
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: September 13, 2005
Last modified: January 25, 2012
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families