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Q48296

- OTCC_HALSA

UniProt

Q48296 - OTCC_HALSA

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Protein
Ornithine carbamoyltransferase, catabolic
Gene
arcB, argB, VNG_6315G
Organism
Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) (Halobacterium halobium)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Reversibly catalyzes the transfer of the carbamoyl group from carbamoyl phosphate (CP) to the N(epsilon) atom of ornithine (ORN) to produce L-citrulline.1 Publication

Catalytic activityi

Carbamoyl phosphate + L-ornithine = phosphate + L-citrulline.UniRule annotation

Enzyme regulationi

Arginine lead to a slight activation. Inhibited by all nucleotide phosphates.1 Publication

Kineticsi

  1. KM=0.4 mM for carbamoyl phosphate1 Publication
  2. KM=8 mM for L-ornithine

pH dependencei

Optimum pH is 8.8. At the physiological pH of 7, activity is only about 30%.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei5 – 51Carbamoyl phosphate By similarity
Sitei26 – 261Important for structural integrity By similarity
Binding sitei65 – 651Carbamoyl phosphate By similarity
Binding sitei76 – 761Carbamoyl phosphate By similarity
Binding sitei100 – 1001Carbamoyl phosphate By similarity
Sitei140 – 1401Important for structural integrity By similarity
Binding sitei155 – 1551Ornithine By similarity
Binding sitei213 – 2131Ornithine By similarity
Binding sitei263 – 2631Carbamoyl phosphate By similarity
Binding sitei281 – 2811Carbamoyl phosphate By similarity

GO - Molecular functioni

  1. amino acid binding Source: InterPro
  2. ornithine carbamoyltransferase activity Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. arginine catabolic process to ornithine Source: UniProtKB-UniPathway
  2. arginine deiminase pathway Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Arginine metabolism

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-662.
UniPathwayiUPA00254; UER00365.

Names & Taxonomyi

Protein namesi
Recommended name:
Ornithine carbamoyltransferase, catabolic (EC:2.1.3.3)
Short name:
cOTCase
Gene namesi
Name:arcB
Synonyms:argB
Ordered Locus Names:VNG_6315G
Encoded oniPlasmid pNRC2001 Publication
OrganismiHalobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) (Halobacterium halobium)
Taxonomic identifieri64091 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeHalobacterium
ProteomesiUP000000554: Plasmid pNRC200

Subcellular locationi

Cytoplasm Inferred UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 295295Ornithine carbamoyltransferase, catabolicUniRule annotation
PRO_0000113063Add
BLAST

Proteomic databases

PaxDbiQ48296.
PRIDEiQ48296.

Interactioni

Subunit structurei

Homohexamer.1 Publication

Protein-protein interaction databases

STRINGi64091.VNG6315G.

Structurei

3D structure databases

ProteinModelPortaliQ48296.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni49 – 535Carbamoyl phosphate binding By similarity
Regioni127 – 1304Carbamoyl phosphate binding By similarity
Regioni217 – 2182Ornithine binding By similarity
Regioni252 – 2554Carbamoyl phosphate binding By similarity

Sequence similaritiesi

Belongs to the ATCase/OTCase family.

Phylogenomic databases

eggNOGiCOG0078.
KOiK00611.
OMAiTAKMMKI.
PhylomeDBiQ48296.

Family and domain databases

Gene3Di3.40.50.1370. 2 hits.
HAMAPiMF_01109. OTCase.
InterProiIPR006132. Asp/Orn_carbamoyltranf_P-bd.
IPR006130. Asp/Orn_carbamoylTrfase.
IPR006131. Asp_carbamoyltransf_Asp/Orn-bd.
IPR002292. Orn/put_carbamltrans.
IPR024904. Orn_carbamltrans.
[Graphical view]
PfamiPF00185. OTCace. 1 hit.
PF02729. OTCace_N. 1 hit.
[Graphical view]
PRINTSiPR00100. AOTCASE.
PR00102. OTCASE.
SUPFAMiSSF53671. SSF53671. 1 hit.
TIGRFAMsiTIGR00658. orni_carb_tr. 1 hit.
PROSITEiPS00097. CARBAMOYLTRANSFERASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q48296-1 [UniParc]FASTAAdd to Basket

« Hide

MEHLVDINDV ESEEIEQLLD LAASMKENPG EFSGVMDNKS LVMLFAKPST    50
RTRLSFETGM TQLGGHGIFF EMGSSQLSRG EPISDVSQVM SRYEDAIMAR 100
LFEHDEMMEL AENADVPVVN GLTDFLHPCQ ALTDMFTMQE KDRLDTLAFV 150
GDGNNVAHSL MQASAKMGVD CRIATPEGME PDEEIQDRVS DANVTVTNDP 200
YEAVDGATAV YGDVFVSMGE EEQREEKLAE FDGFQIDQDL MDAARDDAIF 250
MHCLPAHRGE EVTAEVADGP QSVIFDQAEN RMHVQKAIVH TLVNQ 295
Length:295
Mass (Da):32,676
Last modified:November 1, 1996 - v1
Checksum:iBFF6E002AC0289A2
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X81712 Genomic DNA. Translation: CAA57356.1.
X80931 Genomic DNA. Translation: CAA56906.1.
AE004438 Genomic DNA. Translation: AAG20946.1.
PIRiS49261.
RefSeqiNP_395811.1. NC_002608.1.
WP_010904159.1. NC_002608.1.

Genome annotation databases

EnsemblBacteriaiAAG20946; AAG20946; VNG_6315G.
GeneIDi1449194.
KEGGihal:VNG6315G.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X81712 Genomic DNA. Translation: CAA57356.1 .
X80931 Genomic DNA. Translation: CAA56906.1 .
AE004438 Genomic DNA. Translation: AAG20946.1 .
PIRi S49261.
RefSeqi NP_395811.1. NC_002608.1.
WP_010904159.1. NC_002608.1.

3D structure databases

ProteinModelPortali Q48296.
ModBasei Search...

Protein-protein interaction databases

STRINGi 64091.VNG6315G.

Proteomic databases

PaxDbi Q48296.
PRIDEi Q48296.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAG20946 ; AAG20946 ; VNG_6315G .
GeneIDi 1449194.
KEGGi hal:VNG6315G.

Phylogenomic databases

eggNOGi COG0078.
KOi K00611.
OMAi TAKMMKI.
PhylomeDBi Q48296.

Enzyme and pathway databases

UniPathwayi UPA00254 ; UER00365 .
BioCyci MetaCyc:MONOMER-662.

Family and domain databases

Gene3Di 3.40.50.1370. 2 hits.
HAMAPi MF_01109. OTCase.
InterProi IPR006132. Asp/Orn_carbamoyltranf_P-bd.
IPR006130. Asp/Orn_carbamoylTrfase.
IPR006131. Asp_carbamoyltransf_Asp/Orn-bd.
IPR002292. Orn/put_carbamltrans.
IPR024904. Orn_carbamltrans.
[Graphical view ]
Pfami PF00185. OTCace. 1 hit.
PF02729. OTCace_N. 1 hit.
[Graphical view ]
PRINTSi PR00100. AOTCASE.
PR00102. OTCASE.
SUPFAMi SSF53671. SSF53671. 1 hit.
TIGRFAMsi TIGR00658. orni_carb_tr. 1 hit.
PROSITEi PS00097. CARBAMOYLTRANSFERASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Catabolic ornithine transcarbamylase of Halobacterium halobium (salinarium): purification, characterization, sequence determination, and evolution."
    Ruepp A., Mueller H., Lottspeich F., Soppa J.
    J. Bacteriol. 177:1129-1136(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE, FUNCTION AS A COTCASE, BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION, SUBUNIT.
    Strain: R1 / S9 / L33.
  2. "Fermentative arginine degradation in Halobacterium salinarium (formerly Halobacterium halobium): genes, gene products, and transcripts of the arcRACB gene cluster."
    Ruepp A., Soppa J.
    J. Bacteriol. 178:4942-4947(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: R1 / S9 / L33.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700922 / JCM 11081 / NRC-1.
    Plasmid: pNRC200

Entry informationi

Entry nameiOTCC_HALSA
AccessioniPrimary (citable) accession number: Q48296
Secondary accession number(s): Q59454, Q9HHN0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: September 3, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Not stable below 1 M KCl (1 Publication).

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Plasmid, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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