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Protein

Ornithine carbamoyltransferase, catabolic

Gene

arcB

Organism
Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) (Halobacterium halobium)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Reversibly catalyzes the transfer of the carbamoyl group from carbamoyl phosphate (CP) to the N(epsilon) atom of ornithine (ORN) to produce L-citrulline.1 Publication

Miscellaneous

Not stable below 1 M KCl.1 Publication

Catalytic activityi

Carbamoyl phosphate + L-ornithine = phosphate + L-citrulline.

Enzyme regulationi

Arginine lead to a slight activation. Inhibited by all nucleotide phosphates.1 Publication

Kineticsi

  1. KM=0.4 mM for carbamoyl phosphate1 Publication
  2. KM=8 mM for L-ornithine1 Publication

    pH dependencei

    Optimum pH is 8.8. At the physiological pH of 7, activity is only about 30%.1 Publication

    Pathwayi: L-arginine degradation via ADI pathway

    This protein is involved in step 2 of the subpathway that synthesizes carbamoyl phosphate from L-arginine.
    Proteins known to be involved in the 2 steps of the subpathway in this organism are:
    1. no protein annotated in this organism
    2. Ornithine carbamoyltransferase, catabolic (arcB)
    This subpathway is part of the pathway L-arginine degradation via ADI pathway, which is itself part of Amino-acid degradation.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes carbamoyl phosphate from L-arginine, the pathway L-arginine degradation via ADI pathway and in Amino-acid degradation.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei76Carbamoyl phosphateUniRule annotation1
    Binding sitei100Carbamoyl phosphateUniRule annotation1
    Binding sitei155OrnithineUniRule annotation1
    Binding sitei213OrnithineUniRule annotation1
    Binding sitei281Carbamoyl phosphateUniRule annotation1

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionTransferase
    Biological processArginine metabolism

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-662
    BRENDAi2.1.3.3 2552
    UniPathwayiUPA00254; UER00365

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ornithine carbamoyltransferase, catabolic (EC:2.1.3.3)
    Short name:
    cOTCase
    Gene namesi
    Name:arcB
    Synonyms:argB
    Ordered Locus Names:VNG_6315G
    Encoded oniPlasmid pNRC2001 Publication
    OrganismiHalobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) (Halobacterium halobium)
    Taxonomic identifieri64091 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeHalobacterium
    Proteomesi
    • UP000000554 Componenti: Plasmid pNRC200

    Subcellular locationi

    GO - Cellular componenti

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001130631 – 295Ornithine carbamoyltransferase, catabolicAdd BLAST295

    Proteomic databases

    PRIDEiQ48296

    Interactioni

    Subunit structurei

    Homohexamer.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliQ48296
    SMRiQ48296
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni49 – 52Carbamoyl phosphate bindingUniRule annotation4
    Regioni127 – 130Carbamoyl phosphate bindingUniRule annotation4
    Regioni217 – 218Ornithine bindingUniRule annotation2
    Regioni253 – 254Carbamoyl phosphate bindingUniRule annotation2

    Sequence similaritiesi

    Belongs to the ATCase/OTCase family.Curated

    Phylogenomic databases

    InParanoidiQ48296
    KOiK00611
    OMAiDGNNVCN
    OrthoDBiPOG093Z05BF
    PhylomeDBiQ48296

    Family and domain databases

    Gene3Di3.40.50.1370, 2 hits
    HAMAPiMF_01109 OTCase, 1 hit
    InterProiView protein in InterPro
    IPR006132 Asp/Orn_carbamoyltranf_P-bd
    IPR006130 Asp/Orn_carbamoylTrfase
    IPR036901 Asp/Orn_carbamoylTrfase_sf
    IPR006131 Asp_carbamoyltransf_Asp/Orn-bd
    IPR002292 Orn/put_carbamltrans
    IPR024904 OTCase_ArgI
    PfamiView protein in Pfam
    PF00185 OTCace, 1 hit
    PF02729 OTCace_N, 1 hit
    PRINTSiPR00100 AOTCASE
    PR00102 OTCASE
    SUPFAMiSSF53671 SSF53671, 1 hit
    TIGRFAMsiTIGR00658 orni_carb_tr, 1 hit
    PROSITEiView protein in PROSITE
    PS00097 CARBAMOYLTRANSFERASE, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Q48296-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MEHLVDINDV ESEEIEQLLD LAASMKENPG EFSGVMDNKS LVMLFAKPST
    60 70 80 90 100
    RTRLSFETGM TQLGGHGIFF EMGSSQLSRG EPISDVSQVM SRYEDAIMAR
    110 120 130 140 150
    LFEHDEMMEL AENADVPVVN GLTDFLHPCQ ALTDMFTMQE KDRLDTLAFV
    160 170 180 190 200
    GDGNNVAHSL MQASAKMGVD CRIATPEGME PDEEIQDRVS DANVTVTNDP
    210 220 230 240 250
    YEAVDGATAV YGDVFVSMGE EEQREEKLAE FDGFQIDQDL MDAARDDAIF
    260 270 280 290
    MHCLPAHRGE EVTAEVADGP QSVIFDQAEN RMHVQKAIVH TLVNQ
    Length:295
    Mass (Da):32,676
    Last modified:November 1, 1996 - v1
    Checksum:iBFF6E002AC0289A2
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X81712 Genomic DNA Translation: CAA57356.1
    X80931 Genomic DNA Translation: CAA56906.1
    AE004438 Genomic DNA Translation: AAG20946.1
    PIRiS49261
    RefSeqiWP_010904159.1, NC_002608.1

    Genome annotation databases

    EnsemblBacteriaiAAG20946; AAG20946; VNG_6315G
    GeneIDi5954933
    KEGGihal:VNG_6315G
    PATRICifig|64091.14.peg.2292

    Similar proteinsi

    Entry informationi

    Entry nameiOTCC_HALSA
    AccessioniPrimary (citable) accession number: Q48296
    Secondary accession number(s): Q59454, Q9HHN0
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: November 1, 1996
    Last modified: March 28, 2018
    This is version 123 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Plasmid, Reference proteome
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health