Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ornithine carbamoyltransferase, catabolic

Gene

arcB

Organism
Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) (Halobacterium halobium)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Reversibly catalyzes the transfer of the carbamoyl group from carbamoyl phosphate (CP) to the N(epsilon) atom of ornithine (ORN) to produce L-citrulline.1 Publication

Catalytic activityi

Carbamoyl phosphate + L-ornithine = phosphate + L-citrulline.

Enzyme regulationi

Arginine lead to a slight activation. Inhibited by all nucleotide phosphates.1 Publication

Kineticsi

  1. KM=0.4 mM for carbamoyl phosphate1 Publication
  2. KM=8 mM for L-ornithine1 Publication

    pH dependencei

    Optimum pH is 8.8. At the physiological pH of 7, activity is only about 30%.1 Publication

    Pathway:iL-arginine degradation via ADI pathway

    This protein is involved in step 2 of the subpathway that synthesizes carbamoyl phosphate from L-arginine.
    Proteins known to be involved in the 2 steps of the subpathway in this organism are:
    1. no protein annotated in this organism
    2. Ornithine carbamoyltransferase, catabolic (arcB)
    This subpathway is part of the pathway L-arginine degradation via ADI pathway, which is itself part of Amino-acid degradation.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes carbamoyl phosphate from L-arginine, the pathway L-arginine degradation via ADI pathway and in Amino-acid degradation.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei5 – 51Carbamoyl phosphateBy similarity
    Sitei26 – 261Important for structural integrityBy similarity
    Binding sitei65 – 651Carbamoyl phosphateBy similarity
    Binding sitei76 – 761Carbamoyl phosphateBy similarity
    Binding sitei100 – 1001Carbamoyl phosphateBy similarity
    Sitei140 – 1401Important for structural integrityBy similarity
    Binding sitei155 – 1551OrnithineBy similarity
    Binding sitei213 – 2131OrnithineBy similarity
    Binding sitei263 – 2631Carbamoyl phosphateBy similarity
    Binding sitei281 – 2811Carbamoyl phosphateBy similarity

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Arginine metabolism

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-662.
    BRENDAi2.1.3.3. 2552.
    UniPathwayiUPA00254; UER00365.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ornithine carbamoyltransferase, catabolic (EC:2.1.3.3)
    Short name:
    cOTCase
    Gene namesi
    Name:arcB
    Synonyms:argB
    Ordered Locus Names:VNG_6315G
    Encoded oniPlasmid pNRC2001 Publication
    OrganismiHalobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) (Halobacterium halobium)
    Taxonomic identifieri64091 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeHalobacterium
    ProteomesiUP000000554 Componenti: Plasmid pNRC200

    Subcellular locationi

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 295295Ornithine carbamoyltransferase, catabolicPRO_0000113063Add
    BLAST

    Proteomic databases

    PaxDbiQ48296.
    PRIDEiQ48296.

    Interactioni

    Subunit structurei

    Homohexamer.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliQ48296.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni49 – 535Carbamoyl phosphate bindingBy similarity
    Regioni127 – 1304Carbamoyl phosphate bindingBy similarity
    Regioni217 – 2182Ornithine bindingBy similarity
    Regioni252 – 2554Carbamoyl phosphate bindingBy similarity

    Sequence similaritiesi

    Belongs to the ATCase/OTCase family.Curated

    Phylogenomic databases

    eggNOGiCOG0078.
    InParanoidiQ48296.
    KOiK00611.
    OMAiGIMIRTY.
    PhylomeDBiQ48296.

    Family and domain databases

    Gene3Di3.40.50.1370. 2 hits.
    HAMAPiMF_01109. OTCase.
    InterProiIPR006132. Asp/Orn_carbamoyltranf_P-bd.
    IPR006130. Asp/Orn_carbamoylTrfase.
    IPR006131. Asp_carbamoyltransf_Asp/Orn-bd.
    IPR002292. Orn/put_carbamltrans.
    IPR024904. OTCase_ArgI.
    [Graphical view]
    PfamiPF00185. OTCace. 1 hit.
    PF02729. OTCace_N. 1 hit.
    [Graphical view]
    PRINTSiPR00100. AOTCASE.
    PR00102. OTCASE.
    SUPFAMiSSF53671. SSF53671. 1 hit.
    TIGRFAMsiTIGR00658. orni_carb_tr. 1 hit.
    PROSITEiPS00097. CARBAMOYLTRANSFERASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q48296-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MEHLVDINDV ESEEIEQLLD LAASMKENPG EFSGVMDNKS LVMLFAKPST
    60 70 80 90 100
    RTRLSFETGM TQLGGHGIFF EMGSSQLSRG EPISDVSQVM SRYEDAIMAR
    110 120 130 140 150
    LFEHDEMMEL AENADVPVVN GLTDFLHPCQ ALTDMFTMQE KDRLDTLAFV
    160 170 180 190 200
    GDGNNVAHSL MQASAKMGVD CRIATPEGME PDEEIQDRVS DANVTVTNDP
    210 220 230 240 250
    YEAVDGATAV YGDVFVSMGE EEQREEKLAE FDGFQIDQDL MDAARDDAIF
    260 270 280 290
    MHCLPAHRGE EVTAEVADGP QSVIFDQAEN RMHVQKAIVH TLVNQ
    Length:295
    Mass (Da):32,676
    Last modified:November 1, 1996 - v1
    Checksum:iBFF6E002AC0289A2
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X81712 Genomic DNA. Translation: CAA57356.1.
    X80931 Genomic DNA. Translation: CAA56906.1.
    AE004438 Genomic DNA. Translation: AAG20946.1.
    PIRiS49261.
    RefSeqiNP_395811.1. NC_002608.1.
    WP_010904159.1. NC_002608.1.

    Genome annotation databases

    EnsemblBacteriaiAAG20946; AAG20946; VNG_6315G.
    GeneIDi1449194.
    KEGGihal:VNG6315G.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X81712 Genomic DNA. Translation: CAA57356.1.
    X80931 Genomic DNA. Translation: CAA56906.1.
    AE004438 Genomic DNA. Translation: AAG20946.1.
    PIRiS49261.
    RefSeqiNP_395811.1. NC_002608.1.
    WP_010904159.1. NC_002608.1.

    3D structure databases

    ProteinModelPortaliQ48296.
    ModBaseiSearch...
    MobiDBiSearch...

    Proteomic databases

    PaxDbiQ48296.
    PRIDEiQ48296.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAG20946; AAG20946; VNG_6315G.
    GeneIDi1449194.
    KEGGihal:VNG6315G.

    Phylogenomic databases

    eggNOGiCOG0078.
    InParanoidiQ48296.
    KOiK00611.
    OMAiGIMIRTY.
    PhylomeDBiQ48296.

    Enzyme and pathway databases

    UniPathwayiUPA00254; UER00365.
    BioCyciMetaCyc:MONOMER-662.
    BRENDAi2.1.3.3. 2552.

    Family and domain databases

    Gene3Di3.40.50.1370. 2 hits.
    HAMAPiMF_01109. OTCase.
    InterProiIPR006132. Asp/Orn_carbamoyltranf_P-bd.
    IPR006130. Asp/Orn_carbamoylTrfase.
    IPR006131. Asp_carbamoyltransf_Asp/Orn-bd.
    IPR002292. Orn/put_carbamltrans.
    IPR024904. OTCase_ArgI.
    [Graphical view]
    PfamiPF00185. OTCace. 1 hit.
    PF02729. OTCace_N. 1 hit.
    [Graphical view]
    PRINTSiPR00100. AOTCASE.
    PR00102. OTCASE.
    SUPFAMiSSF53671. SSF53671. 1 hit.
    TIGRFAMsiTIGR00658. orni_carb_tr. 1 hit.
    PROSITEiPS00097. CARBAMOYLTRANSFERASE. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "Catabolic ornithine transcarbamylase of Halobacterium halobium (salinarium): purification, characterization, sequence determination, and evolution."
      Ruepp A., Mueller H., Lottspeich F., Soppa J.
      J. Bacteriol. 177:1129-1136(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE, FUNCTION AS A COTCASE, BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION, SUBUNIT.
      Strain: R1 / S9 / L33.
    2. "Fermentative arginine degradation in Halobacterium salinarium (formerly Halobacterium halobium): genes, gene products, and transcripts of the arcRACB gene cluster."
      Ruepp A., Soppa J.
      J. Bacteriol. 178:4942-4947(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: R1 / S9 / L33.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700922 / JCM 11081 / NRC-1.
      Plasmid: pNRC200

    Entry informationi

    Entry nameiOTCC_HALSA
    AccessioniPrimary (citable) accession number: Q48296
    Secondary accession number(s): Q59454, Q9HHN0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: November 1, 1996
    Last modified: July 22, 2015
    This is version 112 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Not stable below 1 M KCl.1 Publication

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Plasmid, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.