Reviewed, UniProtKB/Swiss-Prot Q48296 (OTCC_HALSA)

Names and origin

Protein names

Recommended name:
    Ornithine carbamoyltransferase, catabolic
      Short name=OTCase
    EC=2.1.3.3

Gene names

Name:	arcB
Synonyms:	argB
Ordered Locus Names:	VNG_6315G

Encoded on

Plasmid pNRC200 Ref.3

Organism

Halobacterium salinarium (Halobacterium halobium) [Complete proteome] [HAMAP]

Taxonomic identifier

2242 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Halobacterium

Protein attributes

Sequence length	295 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Carbamoyl phosphate + L-ornithine = phosphate + L-citrulline. HAMAP MF_01109
Pathway	Amino-acid degradation; L-arginine degradation via ADI pathway; carbamoyl phosphate from L-arginine: step 2/2. HAMAP MF_01109
Subunit structure	Homohexamer. HAMAP MF_01109
Subcellular location	Cytoplasm Probable HAMAP MF_01109.
Sequence similarities	Belongs to the ATCase/OTCase family.

Ontologies

Keywords
Biological process	Arginine metabolism
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome Direct protein sequencing Plasmid
Gene Ontology (GO)
Biological process	arginine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	ornithine carbamoyltransferase complex Inferred from electronic annotation. Source: InterPro
Molecular function	amino acid binding Inferred from electronic annotation. Source: InterPro ornithine carbamoyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 295	295	Ornithine carbamoyltransferase, catabolic HAMAP MF_01109		PRO_0000113063
Regions
	Region	49 – 53	5	Carbamoyl phosphate binding By similarity
	Region	252 – 255	4	Ornithine binding By similarity
Sites
	Binding site	100	1	Carbamoyl phosphate By similarity
	Binding site	127	1	Carbamoyl phosphate By similarity
	Site	26	1	Important for structural integrity By similarity
	Site	140	1	Important for structural integrity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q48296-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: BFF6E002AC0289A2	FASTA	295	32,676
	10 20 30 40 50 60 MEHLVDINDV ESEEIEQLLD LAASMKENPG EFSGVMDNKS LVMLFAKPST RTRLSFETGM 70 80 90 100 110 120 TQLGGHGIFF EMGSSQLSRG EPISDVSQVM SRYEDAIMAR LFEHDEMMEL AENADVPVVN 130 140 150 160 170 180 GLTDFLHPCQ ALTDMFTMQE KDRLDTLAFV GDGNNVAHSL MQASAKMGVD CRIATPEGME 190 200 210 220 230 240 PDEEIQDRVS DANVTVTNDP YEAVDGATAV YGDVFVSMGE EEQREEKLAE FDGFQIDQDL 250 260 270 280 290 MDAARDDAIF MHCLPAHRGE EVTAEVADGP QSVIFDQAEN RMHVQKAIVH TLVNQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Catabolic ornithine transcarbamylase of Halobacterium halobium (salinarium): purification, characterization, sequence determination, and evolution." Ruepp A., Mueller H., Lottspeich F., Soppa J. J. Bacteriol. 177:1129-1136(1995) [PubMed: 7868583] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE, CHARACTERIZATION. Strain: L33.
[2]	"Fermentative arginine degradation in Halobacterium salinarium (formerly Halobacterium halobium): genes, gene products, and transcripts of the arcRACB gene cluster." Ruepp A., Soppa J. J. Bacteriol. 178:4942-4947(1996) [PubMed: 8759859] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: L33.
[3]	"Genome sequence of Halobacterium species NRC-1." Ng W.V., Kennedy S.P., Mahairas G.G., Berquist B., Pan M., Shukla H.D., Lasky S.R., Baliga N.S., Thorsson V., Sbrogna J., Swartzell S., Weir D., Hall J., Dahl T.A., Welti R., Goo Y.A., Leithauser B., Keller K. , Cruz R., Danson M.J., Hough D.W., Maddocks D.G., Jablonski P.E., Krebs M.P., Angevine C.M., Dale H., Isenbarger T.A., Peck R.F., Pohlschroder M., Spudich J.L., Jung K.-H., Alam M., Freitas T., Hou S., Daniels C.J., Dennis P.P., Omer A.D., Ebhardt H., Lowe T.M., Liang P., Riley M., Hood L., DasSarma S. Proc. Natl. Acad. Sci. U.S.A. 97:12176-12181(2000) [PubMed: 11016950] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700922 / JCM 11081 / NRC-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X81712 Genomic DNA. Translation: CAA57356.1. X80931 Genomic DNA. Translation: CAA56906.1. AE004438 Genomic DNA. Translation: AAG20946.1.
PIR	S49261.
RefSeq	NP_395811.1.
3D structure databases
SMR	Q48296. Positions 2-293.
ModBase	Search...
Genome annotation databases
GeneID	1449194.
GenomeReviews	Gene locus VNG_6315G in contig AE004438_GR.
KEGG	hal:VNG6315G.
NMPDR	fig|64091.1.peg.2495.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG579429.
OMA	TVAMIFS.
Enzyme and pathway databases
BioCyc	HSP64091:VNG6315G-MONOMER. MetaCyc:MONOMER-662.
Family and domain databases
HAMAP	MF_01109. OTCase. [Tree]
InterPro	IPR006132. Asp/Orn_carbamoyltranf_P_bd. IPR006130. Asp/Orn_carbamoylTrfase. IPR006131. Asp_carbamoyltransf_Asp/Orn_bd. IPR002292. Orn_carbamltrans. [Graphical view]
Pfam	PF00185. OTCace. 1 hit. PF02729. OTCace_N. 1 hit. [Graphical view]
PRINTS	PR00100. AOTCASE. PR00102. OTCASE.
TIGRFAMs	TIGR00658. orni_carb_tr. 1 hit.
PROSITE	PS00097. CARBAMOYLTRANSFERASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

OTCC_HALSA

Accession

Primary (citable) accession number: Q48296
Secondary accession number(s): Q59454, Q9HHN0

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	February 9, 2010
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)