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Q48296

- OTCC_HALSA

UniProt

Q48296 - OTCC_HALSA

Protein

Ornithine carbamoyltransferase, catabolic

Gene

arcB

Organism
Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) (Halobacterium halobium)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 106 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Reversibly catalyzes the transfer of the carbamoyl group from carbamoyl phosphate (CP) to the N(epsilon) atom of ornithine (ORN) to produce L-citrulline.1 Publication

    Catalytic activityi

    Carbamoyl phosphate + L-ornithine = phosphate + L-citrulline.

    Enzyme regulationi

    Arginine lead to a slight activation. Inhibited by all nucleotide phosphates.1 Publication

    Kineticsi

    1. KM=0.4 mM for carbamoyl phosphate1 Publication
    2. KM=8 mM for L-ornithine1 Publication

    pH dependencei

    Optimum pH is 8.8. At the physiological pH of 7, activity is only about 30%.1 Publication

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei5 – 51Carbamoyl phosphateBy similarity
    Sitei26 – 261Important for structural integrityBy similarity
    Binding sitei65 – 651Carbamoyl phosphateBy similarity
    Binding sitei76 – 761Carbamoyl phosphateBy similarity
    Binding sitei100 – 1001Carbamoyl phosphateBy similarity
    Sitei140 – 1401Important for structural integrityBy similarity
    Binding sitei155 – 1551OrnithineBy similarity
    Binding sitei213 – 2131OrnithineBy similarity
    Binding sitei263 – 2631Carbamoyl phosphateBy similarity
    Binding sitei281 – 2811Carbamoyl phosphateBy similarity

    GO - Molecular functioni

    1. amino acid binding Source: InterPro
    2. ornithine carbamoyltransferase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. arginine catabolic process to ornithine Source: UniProtKB-UniPathway
    2. arginine deiminase pathway Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Arginine metabolism

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-662.
    UniPathwayiUPA00254; UER00365.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ornithine carbamoyltransferase, catabolic (EC:2.1.3.3)
    Short name:
    cOTCase
    Gene namesi
    Name:arcB
    Synonyms:argB
    Ordered Locus Names:VNG_6315G
    Encoded oniPlasmid pNRC2001 Publication
    OrganismiHalobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) (Halobacterium halobium)
    Taxonomic identifieri64091 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeHalobacterium
    ProteomesiUP000000554: Plasmid pNRC200

    Subcellular locationi

    Cytoplasm Curated

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 295295Ornithine carbamoyltransferase, catabolicPRO_0000113063Add
    BLAST

    Proteomic databases

    PaxDbiQ48296.
    PRIDEiQ48296.

    Interactioni

    Subunit structurei

    Homohexamer.1 Publication

    Protein-protein interaction databases

    STRINGi64091.VNG6315G.

    Structurei

    3D structure databases

    ProteinModelPortaliQ48296.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni49 – 535Carbamoyl phosphate bindingBy similarity
    Regioni127 – 1304Carbamoyl phosphate bindingBy similarity
    Regioni217 – 2182Ornithine bindingBy similarity
    Regioni252 – 2554Carbamoyl phosphate bindingBy similarity

    Sequence similaritiesi

    Belongs to the ATCase/OTCase family.Curated

    Phylogenomic databases

    eggNOGiCOG0078.
    KOiK00611.
    OMAiTAKMMKI.
    PhylomeDBiQ48296.

    Family and domain databases

    Gene3Di3.40.50.1370. 2 hits.
    HAMAPiMF_01109. OTCase.
    InterProiIPR006132. Asp/Orn_carbamoyltranf_P-bd.
    IPR006130. Asp/Orn_carbamoylTrfase.
    IPR006131. Asp_carbamoyltransf_Asp/Orn-bd.
    IPR002292. Orn/put_carbamltrans.
    IPR024904. Orn_carbamltrans.
    [Graphical view]
    PfamiPF00185. OTCace. 1 hit.
    PF02729. OTCace_N. 1 hit.
    [Graphical view]
    PRINTSiPR00100. AOTCASE.
    PR00102. OTCASE.
    SUPFAMiSSF53671. SSF53671. 1 hit.
    TIGRFAMsiTIGR00658. orni_carb_tr. 1 hit.
    PROSITEiPS00097. CARBAMOYLTRANSFERASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q48296-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEHLVDINDV ESEEIEQLLD LAASMKENPG EFSGVMDNKS LVMLFAKPST    50
    RTRLSFETGM TQLGGHGIFF EMGSSQLSRG EPISDVSQVM SRYEDAIMAR 100
    LFEHDEMMEL AENADVPVVN GLTDFLHPCQ ALTDMFTMQE KDRLDTLAFV 150
    GDGNNVAHSL MQASAKMGVD CRIATPEGME PDEEIQDRVS DANVTVTNDP 200
    YEAVDGATAV YGDVFVSMGE EEQREEKLAE FDGFQIDQDL MDAARDDAIF 250
    MHCLPAHRGE EVTAEVADGP QSVIFDQAEN RMHVQKAIVH TLVNQ 295
    Length:295
    Mass (Da):32,676
    Last modified:November 1, 1996 - v1
    Checksum:iBFF6E002AC0289A2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X81712 Genomic DNA. Translation: CAA57356.1.
    X80931 Genomic DNA. Translation: CAA56906.1.
    AE004438 Genomic DNA. Translation: AAG20946.1.
    PIRiS49261.
    RefSeqiNP_395811.1. NC_002608.1.
    WP_010904159.1. NC_002608.1.

    Genome annotation databases

    EnsemblBacteriaiAAG20946; AAG20946; VNG_6315G.
    GeneIDi1449194.
    KEGGihal:VNG6315G.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X81712 Genomic DNA. Translation: CAA57356.1 .
    X80931 Genomic DNA. Translation: CAA56906.1 .
    AE004438 Genomic DNA. Translation: AAG20946.1 .
    PIRi S49261.
    RefSeqi NP_395811.1. NC_002608.1.
    WP_010904159.1. NC_002608.1.

    3D structure databases

    ProteinModelPortali Q48296.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 64091.VNG6315G.

    Proteomic databases

    PaxDbi Q48296.
    PRIDEi Q48296.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAG20946 ; AAG20946 ; VNG_6315G .
    GeneIDi 1449194.
    KEGGi hal:VNG6315G.

    Phylogenomic databases

    eggNOGi COG0078.
    KOi K00611.
    OMAi TAKMMKI.
    PhylomeDBi Q48296.

    Enzyme and pathway databases

    UniPathwayi UPA00254 ; UER00365 .
    BioCyci MetaCyc:MONOMER-662.

    Family and domain databases

    Gene3Di 3.40.50.1370. 2 hits.
    HAMAPi MF_01109. OTCase.
    InterProi IPR006132. Asp/Orn_carbamoyltranf_P-bd.
    IPR006130. Asp/Orn_carbamoylTrfase.
    IPR006131. Asp_carbamoyltransf_Asp/Orn-bd.
    IPR002292. Orn/put_carbamltrans.
    IPR024904. Orn_carbamltrans.
    [Graphical view ]
    Pfami PF00185. OTCace. 1 hit.
    PF02729. OTCace_N. 1 hit.
    [Graphical view ]
    PRINTSi PR00100. AOTCASE.
    PR00102. OTCASE.
    SUPFAMi SSF53671. SSF53671. 1 hit.
    TIGRFAMsi TIGR00658. orni_carb_tr. 1 hit.
    PROSITEi PS00097. CARBAMOYLTRANSFERASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Catabolic ornithine transcarbamylase of Halobacterium halobium (salinarium): purification, characterization, sequence determination, and evolution."
      Ruepp A., Mueller H., Lottspeich F., Soppa J.
      J. Bacteriol. 177:1129-1136(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE, FUNCTION AS A COTCASE, BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION, SUBUNIT.
      Strain: R1 / S9 / L33.
    2. "Fermentative arginine degradation in Halobacterium salinarium (formerly Halobacterium halobium): genes, gene products, and transcripts of the arcRACB gene cluster."
      Ruepp A., Soppa J.
      J. Bacteriol. 178:4942-4947(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: R1 / S9 / L33.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700922 / JCM 11081 / NRC-1.
      Plasmid: pNRC200

    Entry informationi

    Entry nameiOTCC_HALSA
    AccessioniPrimary (citable) accession number: Q48296
    Secondary accession number(s): Q59454, Q9HHN0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 106 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Not stable below 1 M KCl.1 Publication

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Plasmid, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3