Q48271 (COPP_HELPY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
COP-associated protein

Alternative name(s):
Copper ion-binding protein

Gene names

Name:	copP
Ordered Locus Names:	HP_1073

Organism

Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) [Reference proteome] [HAMAP]

Taxonomic identifier

85962 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › delta/epsilon subdivisions › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter › Helicobacter pylori

Protein attributes

Sequence length	66 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Part of a cation-transporting system which is associated with copper export out of the H.pylori cells.
Miscellaneous	Copper (2+) binding can oxidyze the Cys residues at the metal-binding site, leading to the formation of a disulfide bond.
Sequence similarities	Contains 1 HMA domain.

Ontologies

Keywords
Ligand	Copper Metal-binding
PTM	Disulfide bond
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	copper ion transport Inferred from electronic annotation. Source: InterPro
Molecular_function	copper ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 66

66

COP-associated protein

PRO_0000079246

Regions

Domain

2 – 66

65

HMA

Sites

Metal binding

12

1

Copper

Metal binding

15

1

Copper

Amino acid modifications

Disulfide bond

12 ↔ 15

Alternate Ref.5

Natural variations

Natural variant

3

1

A → V in strain: A68, NCTC 11638 and NCTC 11639.

Natural variant

13

1

N → D in strain: NCTC 11638.

Natural variant

13

1

N → S in strain: NCTC 11639.

Natural variant

34

1

V → A in strain: A68, NCTC 11638 and NCTC 11639.

Natural variant

35

1

S → N in strain: NCTC 11639.

Natural variant

66

1

V → I in strain: A68, NCTC 11638 and NCTC 11639.

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

66

Helix Strand Turn

Details...

Beta strand

2 – 5

4

Helix

14 – 23

10

Beta strand

26 – 35

10

Turn

36 – 39

4

Beta strand

40 – 45

6

Helix

51 – 61

11

Sequences

	Sequence		Length	Mass (Da)
	Q48271 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: 353E2C3D392816F1	FASTA	66	7,196
	10 20 30 40 50 60 MKATFQVPSI TCNHCVDKIE KFVGEIEGVS FIDVSVEKKS VVVEFDAPAT QDLIKEALLD AGQEVV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and membrane topology of a P type ATPase from Helicobacter pylori." Melchers K., Weitzenegger T., Buhmann A., Steinhilber W., Sachs G., Schaefer K.P. J. Biol. Chem. 271:446-457(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: A68.
[2]	"Nucleotide sequence and mutational analysis indicate that two Helicobacter pylori genes encode a P-type ATPase and a cation-binding protein associated with copper transport." Ge Z., Hiratsuka K., Taylor D.E. Mol. Microbiol. 15:97-106(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 43629 / JCM 7656 / NCTC 11639 / UA802.
[3]	"Identification and characterization of an operon of Helicobacter pylori that is involved in motility and stress adaptation." Beier D., Spohn G., Rappuoli R., Scarlato V. J. Bacteriol. 179:4676-4683(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DSM 4867 / CCUG 17874 / NCTC 11638.
[4]	"The complete genome sequence of the gastric pathogen Helicobacter pylori." Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G., Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A., Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N., Loftus B.J., Richardson D.L., Dodson R.J. , Khalak H.G., Glodek A., McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E., Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D., Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S., Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C. Nature 388:539-547(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700392 / 26695.
[5]	"Structural insight into the distinct properties of copper transport by the Helicobacter pylori CopP protein." Park S.J., Jung Y.-S., Kim J.-S., Seo M.-D., Lee B.J. Proteins 71:1007-1019(2008) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR IN COMPLEX WITH COPPER IONS, DISULFIDE BOND.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U59625 Genomic DNA. Translation: AAB05476.1.
U97567 Genomic DNA. Translation: AAB66381.1.
L33259 Genomic DNA. Translation: AAB67321.1.
AE000511 Genomic DNA. Translation: AAD08120.1.

PIR

A64654.

RefSeq

NP_207864.1. NC_000915.1.
YP_006934989.1. NC_018939.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1YG0	NMR	-	A	1-66	[»]

ProteinModelPortal

Q48271.

SMR

Q48271. Positions 1-66.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-3638N.

MINT

MINT-161359.

STRING

85962.HP1073.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAD08120; AAD08120; HP_1073.

GeneID

13870267.
899609.

KEGG

heo:C694_05545.
hpy:HP1073.

PATRIC

20593485. VBIHelPyl33062_1122.

Phylogenomic databases

eggNOG

COG2608.

OMA

KVTFQVP.

ProtClustDB

CLSK496016.

Family and domain databases

InterPro

IPR000428. Cu-bd.
IPR017969. Heavy-metal-associated_CS.
IPR006121. HeavyMe-assoc_HMA.
IPR006122. HMA_Cu_ion-bd.
[Graphical view]

Pfam

PF00403. HMA. 1 hit.
[Graphical view]

PRINTS

PR00944. CUEXPORT.

SUPFAM

SSF55008. HeavyMe_transpt. 1 hit.

TIGRFAMs

TIGR00003. TIGR00003. 1 hit.

PROSITE

PS01047. HMA_1. 1 hit.
PS50846. HMA_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q48271.

Entry information

Entry name

COPP_HELPY

Accession

Primary (citable) accession number: Q48271
Secondary accession number(s): O07682, Q48257

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 86 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program