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Protein

Vacuolating cytotoxin autotransporter

Gene

vacA

Organism
Helicobacter pylori (Campylobacter pylori)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Induces vacuolation of eukaryotic cells. Causes ulceration and gastric lesions.

GO - Molecular functioni

Keywordsi

Molecular functionToxin

Protein family/group databases

TCDBi1.B.12.6.1. the autotransporter-1 (at-1) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolating cytotoxin autotransporter
Cleaved into the following 2 chains:
Gene namesi
Name:vacA
OrganismiHelicobacter pylori (Campylobacter pylori)
Taxonomic identifieri210 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter

Subcellular locationi

Vacuolating cytotoxin translocator :

GO - Cellular componenti

Keywords - Cellular componenti

Cell outer membrane, Membrane, Periplasm, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000002717? – 1287Vacuolating cytotoxin translocatorSequence analysis
Signal peptidei1 – 331 PublicationAdd BLAST33
ChainiPRO_000038758834 – 1287Vacuolating cytotoxinAdd BLAST1254
ChainiPRO_000000271634 – ?Vacuolating cytotoxin autotransporter

Interactioni

Protein-protein interaction databases

DIPiDIP-46228N.

Structurei

Secondary structure

11287
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi390 – 396Combined sources7
Beta strandi411 – 414Combined sources4
Beta strandi416 – 428Combined sources13
Beta strandi430 – 435Combined sources6
Beta strandi437 – 448Combined sources12
Beta strandi452 – 454Combined sources3
Beta strandi468 – 475Combined sources8
Turni476 – 478Combined sources3
Beta strandi482 – 485Combined sources4
Beta strandi495 – 510Combined sources16
Beta strandi515 – 541Combined sources27
Beta strandi543 – 552Combined sources10
Beta strandi563 – 568Combined sources6
Turni570 – 572Combined sources3
Beta strandi573 – 580Combined sources8
Beta strandi592 – 607Combined sources16
Beta strandi612 – 631Combined sources20
Beta strandi639 – 648Combined sources10
Beta strandi653 – 656Combined sources4
Beta strandi660 – 666Combined sources7
Beta strandi668 – 672Combined sources5
Beta strandi674 – 678Combined sources5
Beta strandi687 – 697Combined sources11
Turni703 – 705Combined sources3
Beta strandi706 – 708Combined sources3
Beta strandi711 – 715Combined sources5
Helixi717 – 719Combined sources3
Beta strandi722 – 730Combined sources9
Beta strandi732 – 738Combined sources7
Beta strandi742 – 745Combined sources4
Helixi751 – 756Combined sources6
Beta strandi759 – 763Combined sources5
Beta strandi769 – 775Combined sources7
Helixi776 – 786Combined sources11
Helixi789 – 793Combined sources5
Helixi795 – 801Combined sources7
Beta strandi804 – 807Combined sources4
Beta strandi811 – 815Combined sources5
Beta strandi817 – 824Combined sources8
Beta strandi837 – 839Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1SEWmodel-A/B/C/D/E/F39-62[»]
2QV3X-ray2.40A388-844[»]
ProteinModelPortaliQ48245.
SMRiQ48245.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ48245.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1014 – 1287AutotransporterPROSITE-ProRule annotationAdd BLAST274

Domaini

The signal peptide, cleaved at the inner membrane, guides the autotransporter protein to the periplasmic space. Then, insertion of the C-terminal translocator domain in the outer membrane forms a hydrophilic pore for the translocation of the passenger domain to the bacterial cell surface, with subsequent cleavage (By similarity).By similarity

Keywords - Domaini

Signal, Transmembrane, Transmembrane beta strand

Family and domain databases

InterProiView protein in InterPro
IPR005546. Autotransporte_beta.
IPR036709. Autotransporte_beta_dom_sf.
IPR006315. OM_autotransptr_brl.
IPR003842. Vacuolating_cytotoxin.
PfamiView protein in Pfam
PF02691. VacA. 1 hit.
PRINTSiPR01656. VACCYTOTOXIN.
SMARTiView protein in SMART
SM00869. Autotransporter. 1 hit.
SUPFAMiSSF103515. SSF103515. 1 hit.
TIGRFAMsiTIGR01414. autotrans_barl. 1 hit.
PROSITEiView protein in PROSITE
PS51208. AUTOTRANSPORTER. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q48245-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEIQQTHRKI NRPLVSLALV GALVSITPQQ SHAAFFTTVI IPAIVGGIAT
60 70 80 90 100
GTAVGTVSGL LGWGLKQAEE ANKTPDKPDK VWRIQAGKGF NEFPNKEYDL
110 120 130 140 150
YKSLLSSKID GGWDWGNAAT HYWIKGGQWN KLEVDMKDAV GTYKLSGLRN
160 170 180 190 200
FTGGDLDVNM QKATLRLGQF NGNSFTSYKD SADRTTRVDF NAKNILIDNF
210 220 230 240 250
LEINNRVGSG AGRKASSTVL TLQASEGITS SKNAEISLYD GATLNLASNS
260 270 280 290 300
VKLNGNVWMG RLQYVGAYLA PSYSTINTSK VTGEVNFNHL TVGDHNAAQA
310 320 330 340 350
GIIASNKTHI GTLDLWQSAG LNIIAPPEGG YKDKPNNTPS QSGAKNDKQE
360 370 380 390 400
SSQNNSNTQV INPPNSTQKT EVQPTQVIDG PFAGGKDTVV NIDRINTKAD
410 420 430 440 450
GTIKVGGFKA SLTTNAAHLN IGKGGVNLSN QASGRTLLVE NLTGNITVDG
460 470 480 490 500
PLRVNNQVGG YALAGSSANF EFKAGVDTKN GTATFNNDIS LGRFVNLKVD
510 520 530 540 550
AHTANFKGID TGNGGFNTLD FSGVTNKVNI NKLITASTNV AVKNFNINEL
560 570 580 590 600
IVKTNGVSVG EYTHFSEDIG SQSRINTVRL ETGTRSIFSG GVKFKSGEKL
610 620 630 640 650
VIDEFYYSPW NYFDARNIKN VEITRKFASS TPENPWGTSK LMFNNLTLGQ
660 670 680 690 700
NAVMDYSQFS NLTIQGDFIN NQGTINYLVR GGKVATLNVG NAAAMMFNND
710 720 730 740 750
IDSATGFYKP LIKINSAQDL IKNTEHVLLK AKIIGYGNVS TGTNGISNVN
760 770 780 790 800
LEEQFKERLA LYNNNNRMDT CVVRNTDDIK ACGMAIGNQS MVNNPDNYKY
810 820 830 840 850
LIGKAWKNIG ISKTANGSKI SVYYLGNSTP TENGGNTTNL PTNTTNNARF
860 870 880 890 900
ASYALIKNAP FAHSATPNLV AINQHDFGTI ESVFELANRS KDIDTLYANS
910 920 930 940 950
GAQGRDLLQT LLIDSHDAGY ARTMIDATSA NEITKQLNTA TTTLNNIASL
960 970 980 990 1000
EHKTSSLQTL SLSNAMILNS RLVNLSRRHT NNIDSFAKRL QALKDQRFAS
1010 1020 1030 1040 1050
LESAAEVLYQ FAPKYEKPTN VWANAIGGAS LNNGGNASLY GTSAGVDAYL
1060 1070 1080 1090 1100
NGQVEAIVGG FGSYGYSSFN NQANSLNSGA NNTNFGVYSR IFANQHEFDF
1110 1120 1130 1140 1150
EAQGALGSDQ SSLNFKSALL RDLNQSYNYL AYSAATRASY GYDFAFFRNA
1160 1170 1180 1190 1200
LVLKPSVGVS YNHLGSTNFK SNSTNKVALS NGSSSQHLFN ASANVEARYY
1210 1220 1230 1240 1250
YGDTSYFYMN AGVLQEFANF GSSNAVSLNT FKVNATRNPL NTHARVMMGG
1260 1270 1280
ELKLAKEVFL NLGVVYLHNL ISNIGHFASN LGMRYSF
Length:1,287
Mass (Da):139,041
Last modified:November 1, 1996 - v1
Checksum:i0007370062FCB71F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U05676 Unassigned DNA. Translation: AAA17657.1.
PIRiB53739.

Similar proteinsi

Entry informationi

Entry nameiVACA2_HELPX
AccessioniPrimary (citable) accession number: Q48245
Secondary accession number(s): Q9R5K9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: November 22, 2017
This is version 83 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references