Q48211 (LST_HAEIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase
Short name=Alpha 2,3-ST
Short name=Beta-galactoside alpha-2,3-sialyltransferase
EC=2.4.99.-

Alternative name(s):
Lipooligosaccharide sialyltransferase

Gene names

Name:	lst
Ordered Locus Names:	HI_1699

Organism

Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)

Taxonomic identifier

71421 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	304 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Transfers sialic acid from the substrate CMP-sialic acid donor to the terminal beta-D-galactosyl-1,4-acetyl-beta-D-glucosamine on the lacto-N-neotetraose branch of the lipooligosaccharide.
Catalytic activity	CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-acetyl-beta-D-glucosamine = CMP + alpha-N-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine.
Pathway	Bacterial outer membrane biogenesis; lipopolysaccharide biosynthesis.
Sequence similarities	Belongs to the glycosyltransferase 52 family.

Ontologies

Keywords
Biological process	Lipopolysaccharide biosynthesis
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	lipopolysaccharide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	transferase activity, transferring glycosyl groups Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 304	304	CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase		PRO_0000080571
Experimental info
	Sequence conflict	4	1	M → I in AAA24979. Ref.1
	Sequence conflict	68	1	F → S in AAA24979. Ref.1
	Sequence conflict	130	1	G → D in AAA24979. Ref.1
	Sequence conflict	220	1	Y → C in AAA24979. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q48211 [UniParc]. Last modified June 1, 2001. Version 2. Checksum: B6D03890AC1CDD28	FASTA	304	35,706
	10 20 30 40 50 60 MNLMLCCTPL QVLIARKIIE LHPNEQFFGV MFGGVWDKKR TLYASKLAEV CSDSMNIDTG 70 80 90 100 110 120 KDLKGFDFLK LMRQLKNKIT HKGFDKVFLA NLNSLWLQTY LSHVSFKELY TFDDGSDNIF 130 140 150 160 170 180 PHPNLLREPG TFKYKLIKAF IGDKYSVNKL FKKIKKHYTV YPNYKNIVSN IEPISLWDNQ 190 200 210 220 230 240 IDCEIDGEVS FFIGQPLLNT KEENISLIKK LKDQIPFDYY FPHPAEDYRV DGVNYVESEL 250 260 270 280 290 300 IFEDYVFKHL SNKKIIIYTF FSSVAFNLLS HPNVEIRFIR TSIPRWQFCY DSFPDLGLTI YKEI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization and sequence of the lsg locus from Haemophilus influenzae." McLaughlin R., Abu Kwaik Y., Young R., Spinola S., Apicella M. Submitted (JUN-1992) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: A2.
[2]	"Whole-genome random sequencing and assembly of Haemophilus influenzae Rd." Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F., Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M., McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D., Scott J.D., Shirley R., Liu L.-I. , Glodek A., Kelley J.M., Weidman J.F., Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R., Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D., Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A., Small K.V., Fraser C.M., Smith H.O., Venter J.C. Science 269:496-512(1995) [PubMed: 7542800] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M94855 Genomic DNA. Translation: AAA24979.1. L42023 Genomic DNA. Translation: AAC23345.1.
PIR	G64175.
RefSeq	NP_439841.1. NC_000907.1.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT52. Glycosyltransferase Family 52.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	950520.
GenomeReviews	Gene locus HI_1699 in contig L42023_GR.
KEGG	hin:HI1699.
PATRIC	20192149. VBIHaeInf48452_1778.
TIGR	HI_1699.
Phylogenomic databases
HOGENOM	HBG495263.
OMA	ERIMAQH.
ProtClustDB	CLSK870133.
Enzyme and pathway databases
BioCyc	HINF71421:HI_1699-MONOMER.
Family and domain databases
InterPro	IPR012477. Glyco_transf_52. [Graphical view]
KO	K00785.
Pfam	PF07922. Glyco_transf_52. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LST_HAEIN

Accession

Primary (citable) accession number: Q48211
Secondary accession number(s): O05084

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 2001
Last sequence update:	June 1, 2001
Last modified:	January 25, 2012
This is version 61 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program