Reviewed, UniProtKB/Swiss-Prot Q481S6 (GLYA2_COLP3)

Names and origin

Protein names

Recommended name:
    Serine hydroxymethyltransferase 2
      Short name=Serine methylase 2
      Short name=SHMT 2
    EC=2.1.2.1

Gene names

Name:	glyA2
Ordered Locus Names:	CPS_2477

Organism

Colwellia psychrerythraea (strain 34H / ATCC BAA-681) (Vibrio psychroerythus) [Complete proteome] [HAMAP]

Taxonomic identifier

167879 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Colwelliaceae › Colwellia

Protein attributes

Sequence length	417 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Interconversion of serine and glycine. HAMAP MF_00051
Catalytic activity	5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine. HAMAP MF_00051
Cofactor	Pyridoxal phosphate By similarity. HAMAP MF_00051
Pathway	One-carbon metabolism; tetrahydrofolate interconversion. HAMAP MF_00051
Subunit structure	Homotetramer By similarity. HAMAP MF_00051
Subcellular location	Cytoplasm By similarity HAMAP MF_00051.
Sequence similarities	Belongs to the SHMT family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	L-serine metabolic process Inferred from electronic annotation. Source: InterPro glycine metabolic process Inferred from electronic annotation. Source: InterPro one-carbon metabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glycine hydroxymethyltransferase activity Inferred from electronic annotation. Source: HAMAP pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 417	417	Serine hydroxymethyltransferase 2 HAMAP MF_00051		PRO_0000234968
Amino acid modifications
	Modified residue	230	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q481S6-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 492CADB4737489D5	FASTA	417	45,458
	10 20 30 40 50 60 MFYKNDQIAG FDDSIWQAME QEDKRQQDHV ELIASENYTS ARVMQAQGSQ LTNKYAEGYP 70 80 90 100 110 120 GKRYYGGCEH VDVIEQLAID RAKELFGADY ANVQPHSGSQ ANAAVFMALL KPGETVLGMS 130 140 150 160 170 180 LAHGGHLTHG SKVSFSGKIY NAVQYGLNEA TGEIDYEEVE RLAKEHQPKM IIAGFSAYSR 190 200 210 220 230 240 VVDWQRFRDI ADSIGAWLFV DMAHVAGLVA AGLYPNPVPI ADVVTTTTHK TLRGPRGGLI 250 260 270 280 290 300 LAKQNDELAK KLNSAVFPAG QGGPLMHVIA AKAICFKEAL GEGYVEYQQQ VIDNAREMAK 310 320 330 340 350 360 TFQTRGYNVV SGGTDNHLFL LDLIDKGITG KDADAALGRA NITVNKNSVP NDPQSPFVTS 370 380 390 400 410 GLRIGTPAIT SRGFGLEEAA ALTGWICDVL DDISNEQVID DVRSKVLDLC EKNPVYR

References

[1]

"The psychrophilic lifestyle as revealed by the genome sequence of Colwellia psychrerythraea 34H through genomic and proteomic analyses." Methe B.A., Nelson K.E., Deming J.W., Momen B., Melamud E., Zhang X., Moult J., Madupu R., Nelson W.C., Dodson R.J., Brinkac L.M., Daugherty S.C., Durkin A.S., DeBoy R.T., Kolonay J.F., Sullivan S.A., Zhou L., Davidsen T.M. , Wu M., Huston A.L., Lewis M., Weaver B., Weidman J.F., Khouri H., Utterback T.R., Feldblyum T.V., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 102:10913-10918(2005) [PubMed: 16043709] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000083 Genomic DNA. Translation: AAZ25763.1.
RefSeq	YP_269193.1.
3D structure databases
SMR	Q481S6. Positions 1-416.
ModBase	Search...
Protein-protein interaction databases
STRING	Q481S6.
Genome annotation databases
GeneID	3520009.
GenomeReviews	Gene locus CPS_2477 in contig CP000083_GR.
KEGG	cps:CPS_2477.
NMPDR	fig|167879.3.peg.2411.
TIGR	CPS_2477.
Phylogenomic databases
eggNOG	COG0112.
HOGENOM	HBG301263.
Enzyme and pathway databases
BioCyc	CPSY167879:CPS_2477-MONOMER.
Family and domain databases
HAMAP	MF_00051_B. SHMT_B. [Tree]
InterPro	IPR015424. PyrdxlP-dep_Trfase_major_dom. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR001085. Ser_HO-MeTrfase. IPR019798. Ser_HO-MeTrfase_PLP_BS. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
PANTHER	PTHR11680. Gly_HO-Metrfase. 1 hit.
Pfam	PF00464. SHMT. 1 hit. [Graphical view]
PIRSF	PIRSF000412. SHMT. 1 hit.
PROSITE	PS00096. SHMT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLYA2_COLP3

Accession

Primary (citable) accession number: Q481S6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2006
Last sequence update:	September 13, 2005
Last modified:	January 19, 2010
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)