Q481G3 (SYN_COLP3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Asparagine--tRNA ligase
EC=6.1.1.22

Alternative name(s):
Asparaginyl-tRNA synthetase
Short name=AsnRS

Gene names

Name:	asnS
Ordered Locus Names:	CPS_2591

Organism

Colwellia psychrerythraea (strain 34H / ATCC BAA-681) (Vibrio psychroerythus) [Complete proteome] [HAMAP]

Taxonomic identifier

167879 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Colwelliaceae › Colwellia

Protein attributes

Sequence length	466 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + L-asparaginyl-tRNA(Asn). HAMAP MF_00534
Subunit structure	Homodimer By similarity. HAMAP MF_00534
Subcellular location	Cytoplasm HAMAP MF_00534.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	asparaginyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW asparagine-tRNA ligase activity Inferred from electronic annotation. Source: EC nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 466	466	Asparagine--tRNA ligase HAMAP MF_00534		PRO_1000051388

Sequences

	Sequence		Length	Mass (Da)
	Q481G3 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: D2D1030A3F062DD7	FASTA	466	52,401
	10 20 30 40 50 60 MSVISITDVL AGNFPVNESI TIHGWIRTRR DSKAGISFLA LHDGSCFDAI QAIVPNELDN 70 80 90 100 110 120 YESDVLKLTT GCSVKVTGIL VESPGKGQAF EIQATEVEVL GFVEDPDTYP MAAKRHSIEF 130 140 150 160 170 180 LREQAHLRPR TNIGGAVTRV RNCLAQAVHR FLHSKGYFWI STPLITGSDC EGAGEMFRVS 190 200 210 220 230 240 TLDMENLPRN DEGKVDYNKD FFGKETFLTV SGQLNVETYC NALSKVYTFG PTFRAENSNT 250 260 270 280 290 300 TRHLAEFWMV EPEIAFADLS DAADLAEEML KYVFKAVLEE RPDDMAFFQQ RVDKTVLDRL 310 320 330 340 350 360 NSVINTDFVR LDYTDAITIL ENCGKKFENQ VSWGVDLNSE HERYLAEEHF NGPVVLQNYP 370 380 390 400 410 420 KDIKSFYMRL NDDGKTVAAM DILAPGIGEI IGGSQREERL DVLDSRLEEM GLDIADYGWY 430 440 450 460 RDLRRYGTVP HSGFGLGFER LVAYATGMQN VRDVIPFPRT PNNAAF

References

[1]

"The psychrophilic lifestyle as revealed by the genome sequence of Colwellia psychrerythraea 34H through genomic and proteomic analyses." Methe B.A., Nelson K.E., Deming J.W., Momen B., Melamud E., Zhang X., Moult J., Madupu R., Nelson W.C., Dodson R.J., Brinkac L.M., Daugherty S.C., Durkin A.S., DeBoy R.T., Kolonay J.F., Sullivan S.A., Zhou L., Davidsen T.M. , Wu M., Huston A.L., Lewis M., Weaver B., Weidman J.F., Khouri H., Utterback T.R., Feldblyum T.V., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 102:10913-10918(2005) [PubMed: 16043709] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 34H / ATCC BAA-681.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000083 Genomic DNA. Translation: AAZ27879.1.
RefSeq	YP_269306.1. NC_003910.7.
3D structure databases
HSSP	HSSP built from PDB template 1X56 based on UniProtKB O57980.
ProteinModelPortal	Q481G3.
ModBase	Search...
Protein-protein interaction databases
STRING	Q481G3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3522138.
GenomeReviews	Gene locus CPS_2591 in contig CP000083_GR.
KEGG	cps:CPS_2591.
NMPDR	fig|167879.3.peg.2872.
PATRIC	21468243. VBIColPsy94388_2355.
TIGR	CPS_2591.
Phylogenomic databases
eggNOG	COG0017.
HOGENOM	HBG745843.
OMA	AIHRFFH.
ProtClustDB	PRK03932.
Enzyme and pathway databases
BioCyc	CPSY167879:CPS_2591-MONOMER.
Family and domain databases
HAMAP	MF_00534. Asn_tRNA_synth. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II. IPR004522. Asn-tRNA-synth_IIb. IPR002312. Asp/Asn-tRNA-synth_IIb. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR004365. NA-bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KO	K01893.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit. PTHR22594:SF6. PTHR22594:SF6. 1 hit.
Pfam	PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit.
TIGRFAMs	TIGR00457. AsnS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYN_COLP3

Accession

Primary (citable) accession number: Q481G3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 13, 2005
Last modified:	January 25, 2012
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program