Reviewed, UniProtKB/Swiss-Prot Q47Z78 (PUR5_COLP3)

Names and origin

Protein names

Recommended name:
    Phosphoribosylformylglycinamidine cyclo-ligase
    EC=6.3.3.1
Alternative name(s):
    AIRS
    Phosphoribosyl-aminoimidazole synthetase
    AIR synthase

Gene names

Name:	purM
Ordered Locus Names:	CPS_3196

Organism

Colwellia psychrerythraea (strain 34H / ATCC BAA-681) (Vibrio psychroerythus) [Complete proteome] [HAMAP]

Taxonomic identifier

167879 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Colwelliaceae › Colwellia

Protein attributes

Sequence length	346 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole. HAMAP MF_00741
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/5. HAMAP MF_00741
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the AIR synthase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine cyclo-ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 346	346	Phosphoribosylformylglycinamidine cyclo-ligase HAMAP MF_00741		PRO_0000258348

Sequences

	Sequence		Length	Mass (Da)
	Q47Z78-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: D2E9B0EFE11AC0DC	FASTA	346	36,849
	10 20 30 40 50 60 MSEQKQSLSY KDAGVDIDAG NALVENIKGA VKRTTRPEVM GGLGGFGSVC QLPTGYKEPV 70 80 90 100 110 120 LVAGTDGVGT KLRLAIDLAK HDTVGIDLVA MCVNDLIVQG AEPLFFLDYY ATAKLDVAVA 130 140 150 160 170 180 SSVVEGIAEG CIQSGCALVG GETAEMPGMY HKGDYDIAGF CVGVAEKSRL IDGTNVAAGD 190 200 210 220 230 240 QLIALGASGP HSNGFSLIRK VLEVNNTDTN ELLEGKKIAD HLLEPTKIYV KSVLELLKNV 250 260 270 280 290 300 DVHALSHITG GGFWENIPRV LPETAQAVIK GDSWQWPSIF NWLQENGNIT EHEMYRTFNC 310 320 330 340 GVGMVIVVPA DKVAQSIEVL TAHGENAWHL GEIADKADGE EQVVFA

References

[1]

"The psychrophilic lifestyle as revealed by the genome sequence of Colwellia psychrerythraea 34H through genomic and proteomic analyses." Methe B.A., Nelson K.E., Deming J.W., Momen B., Melamud E., Zhang X., Moult J., Madupu R., Nelson W.C., Dodson R.J., Brinkac L.M., Daugherty S.C., Durkin A.S., DeBoy R.T., Kolonay J.F., Sullivan S.A., Zhou L., Davidsen T.M. , Wu M., Huston A.L., Lewis M., Weaver B., Weidman J.F., Khouri H., Utterback T.R., Feldblyum T.V., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 102:10913-10918(2005) [PubMed: 16043709] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000083 Genomic DNA. Translation: AAZ25488.1.
RefSeq	YP_269892.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3519204.
GenomeReviews	Gene locus CPS_3196 in contig CP000083_GR.
KEGG	cps:CPS_3196.
NMPDR	fig|167879.3.peg.2832.
TIGR	CPS_3196.
Phylogenomic databases
HOGENOM	Q47Z78.
OMA	Q47Z78. CGKLDPE.
Enzyme and pathway databases
BioCyc	CPSY167879:CPS_3196-MON.
Family and domain databases
HAMAP	MF_00741. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR004733. PurM_cligase. [Graphical view]
Pfam	PF00586. AIRS. 1 hit. PF02769. AIRS_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR00878. purM. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PUR5_COLP3

Accession

Primary (citable) accession number: Q47Z78

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	September 13, 2005
Last modified:	June 16, 2009
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)