Q47Z40 (SYQ_COLP3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamine--tRNA ligase
EC=6.1.1.18

Alternative name(s):
Glutaminyl-tRNA synthetase
Short name=GlnRS

Gene names

Name:	glnS
Ordered Locus Names:	CPS_3240

Organism

Colwellia psychrerythraea (strain 34H / ATCC BAA-681) (Vibrio psychroerythus) [Complete proteome] [HAMAP]

Taxonomic identifier

167879 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Colwelliaceae › Colwellia

Protein attributes

Sequence length	556 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln). HAMAP MF_00126
Subunit structure	Monomer By similarity. HAMAP MF_00126
Subcellular location	Cytoplasm By similarity HAMAP MF_00126.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutaminyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 556	556	Glutamine--tRNA ligase HAMAP MF_00126		PRO_1000095486
Regions
	Motif	34 – 44	11	"HIGH" region HAMAP MF_00126
	Motif	268 – 272	5	"KMSKS" region HAMAP MF_00126
Sites
	Binding site	271	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q47Z40 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: DD3778A8708F20FD	FASTA	556	63,750
	10 20 30 40 50 60 MSDTENRPTN FIRNIIDADL DSGKHTGVQT RFPPEPNGFL HIGHAKAICL NFGIAQDYNG 70 80 90 100 110 120 LCNLRFDDTN PEKEDIDYVH AIQKDVKWLG FEWAGEIHYS SNYFDQLHGF AVELIEKGLA 130 140 150 160 170 180 YVCFLNAEET REYRGTLNKP GKNSPYRDTS VEENLALFAK MKNGEFEEGI CALRAKIDMT 190 200 210 220 230 240 SSFMCLRDPI IYRVRFAHHH QTGDKWCIYP MYDFTHCLSD ALEGITHSIC TLEFQDNRRL 250 260 270 280 290 300 YDWVIEQVSV PSTPHQYEFS RLNLEYTLMS KRKLNTLVEE KLVDSWDDPR MPTIAAFRRR 310 320 330 340 350 360 GYTPASMREF AKRIGVTKME NTIEMSVLEA CIREDLNDNA PRAMAVLDPI KLVIENYPED 370 380 390 400 410 420 KNEDLIVKNH PSDDEQGTRI VPFSKELYIE AEDFREEANK KYKRLVIDKA VRLRGAYVVT 430 440 450 460 470 480 ATRCDKDEQG NVTTVYCTYN EDTLGKNPTD GTKPKGVIHW VDANKSLDAI VRLYDRLFTV 490 500 510 520 530 540 PNPAAADDFN SVINPQSLVT ITGAKVEPSL AEAKPEFAYQ FERQGYFCLD NDIKEPGALV 550 FNRTVGLRDT WAKISQ

References

[1]

"The psychrophilic lifestyle as revealed by the genome sequence of Colwellia psychrerythraea 34H through genomic and proteomic analyses." Methe B.A., Nelson K.E., Deming J.W., Momen B., Melamud E., Zhang X., Moult J., Madupu R., Nelson W.C., Dodson R.J., Brinkac L.M., Daugherty S.C., Durkin A.S., DeBoy R.T., Kolonay J.F., Sullivan S.A., Zhou L., Davidsen T.M. , Wu M., Huston A.L., Lewis M., Weaver B., Weidman J.F., Khouri H., Utterback T.R., Feldblyum T.V., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 102:10913-10918(2005) [PubMed: 16043709] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 34H / ATCC BAA-681.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000083 Genomic DNA. Translation: AAZ27222.1.
RefSeq	YP_269930.1. NC_003910.7.
3D structure databases
ProteinModelPortal	Q47Z40.
SMR	Q47Z40. Positions 9-550.
ModBase	Search...
Protein-protein interaction databases
STRING	Q47Z40.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3521478.
GenomeReviews	Gene locus CPS_3240 in contig CP000083_GR.
KEGG	cps:CPS_3240.
NMPDR	fig|167879.3.peg.3106.
PATRIC	21469457. VBIColPsy94388_2942.
TIGR	CPS_3240.
Phylogenomic databases
eggNOG	COG0008.
HOGENOM	HBG334108.
OMA	RMPTIAG.
ProtClustDB	PRK05347.
Enzyme and pathway databases
BioCyc	CPSY167879:CPS_3240-MONOMER.
Family and domain databases
HAMAP	MF_00126. Gln_tRNA_synth. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR004514. Gln-tRNA-synth_Ib. IPR022861. Gln_tRNA_synth_bac. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd. IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl. IPR011035. Ribosomal_L25/Gln-tRNA_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:2.40.240.10. Rbsml_L25/Gln-tRNA_synth_b-brl. 2 hits. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KO	K01886.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. PF03950. tRNA-synt_1c_C. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF50715. Ribosomal_L25rel. 1 hit.
TIGRFAMs	TIGR00440. GlnS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYQ_COLP3

Accession

Primary (citable) accession number: Q47Z40

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	September 13, 2005
Last modified:	January 25, 2012
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program