Reviewed, UniProtKB/Swiss-Prot Q47YB3 (QUEF_COLP3)

Names and origin

Protein names

Recommended name:
    NADPH-dependent 7-cyano-7-deazaguanine reductase
    EC=1.7.1.13
Alternative name(s):
    7-cyano-7-carbaguanine reductase
    PreQ(0) reductase
    NADPH-dependent nitrile oxidoreductase

Gene names

Name:	queF
Ordered Locus Names:	CPS_3533

Organism

Colwellia psychrerythraea (strain 34H / ATCC BAA-681) (Vibrio psychroerythus) [Complete proteome] [HAMAP]

Taxonomic identifier

167879 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Colwelliaceae › Colwellia

Protein attributes

Sequence length	286 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) By similarity.
Catalytic activity	7-aminomethyl-7-carbaguanine + 2 NADP(+) = 7-cyano-7-carbaguanine + 2 NADPH.
Pathway	tRNA modification; queuosine-tRNA biosynthesis.
Subcellular location	CytoplasmProbable.
Sequence similarities	Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily.

Ontologies

Keywords
Biological process	Queuosine biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic compound biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW queuosine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	GTP cyclohydrolase I activity Inferred from electronic annotation. Source: InterPro oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor Inferred from electronic annotation. Source: HAMAP queuine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 286	286	NADPH-dependent 7-cyano-7-deazaguanine reductase		PRO_0000163027

Sequences

	Sequence		Length	Mass (Da)
	Q47YB3-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 08C25CF58576F6A8	FASTA	286	32,614
	10 20 30 40 50 60 MANYQNATEL SKLTLGKSTQ YCSEYTADLL QGVPRSLNRD DLALNQSNLP FVGEDVWYGY 70 80 90 100 110 120 ELSWLNGKGK PVVAVAEFRF ACTSDNIVES KSFKLYLNSF NQTRFSSIKD VEKVLTKDLS 130 140 150 160 170 180 KIAGSEASVN LFGVDHCPAL DIAKKSDKCI CIDGEDISID NYQYDPQLLA TAQDERSGSQ 190 200 210 220 230 240 IEEYLVSHLL KSNCLITNQP DWASIYIHYR GKAIDHSSLL KYLISFRQHN EFHEQCVERI 250 260 270 280 YCDLQQFCQL DELTIFARYT RRGGLDINPF RSSHIEQAPF ARTLRQ

References

[1]

"The psychrophilic lifestyle as revealed by the genome sequence of Colwellia psychrerythraea 34H through genomic and proteomic analyses." Methe B.A., Nelson K.E., Deming J.W., Momen B., Melamud E., Zhang X., Moult J., Madupu R., Nelson W.C., Dodson R.J., Brinkac L.M., Daugherty S.C., Durkin A.S., DeBoy R.T., Kolonay J.F., Sullivan S.A., Zhou L., Davidsen T.M. , Wu M., Huston A.L., Lewis M., Weaver B., Weidman J.F., Khouri H., Utterback T.R., Feldblyum T.V., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 102:10913-10918(2005) [PubMed: 16043709] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000083 Genomic DNA. Translation: AAZ24878.1.
RefSeq	YP_270207.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3518726.
GenomeReviews	Gene locus CPS_3533 in contig CP000083_GR.
KEGG	cps:CPS_3533.
NMPDR	fig|167879.3.peg.3298.
TIGR	CPS_3533.
Phylogenomic databases
HOGENOM	Q47YB3.
Enzyme and pathway databases
BioCyc	CPSY167879:CPS_3533-MON.
Family and domain databases
HAMAP	MF_00817. [Tree]
InterPro	IPR016428. CN_OxRdtase_NADPH-dep_YqcD. IPR001474. GTP_CycOHase_I. [Graphical view]
Pfam	PF01227. GTP_cyclohydroI. 1 hit. [Graphical view]
PIRSF	PIRSF004750. Nitrile_oxidored_YqcD_prd. 1 hit.
TIGRFAMs	TIGR03138. QueF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

QUEF_COLP3

Accession

Primary (citable) accession number: Q47YB3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	September 13, 2005
Last modified:	November 25, 2008
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)