Reviewed, UniProtKB/Swiss-Prot Q47Y99 (KDSA_COLP3)

Names and origin

Protein names

Recommended name:
    2-dehydro-3-deoxyphosphooctonate aldolase
    EC=2.5.1.55
Alternative name(s):
    Phospho-2-dehydro-3-deoxyoctonate aldolase
    3-deoxy-D-manno-octulosonic acid 8-phosphate synthetase
    KDO-8-phosphate synthetase
      Short name=KDO 8-P synthase
      Short name=KDOPS

Gene names

Name:	kdsA
Ordered Locus Names:	CPS_3547

Organism

Colwellia psychrerythraea (strain 34H / ATCC BAA-681) (Vibrio psychroerythus) [Complete proteome] [HAMAP]

Taxonomic identifier

167879 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Colwelliaceae › Colwellia

Protein attributes

Sequence length	280 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Phosphoenolpyruvate + D-arabinose 5-phosphate + H2O = 2-dehydro-3-deoxy-D-octonate 8-phosphate + phosphate. HAMAP MF_00056
Pathway	Carbohydrate biosynthesis; 3-deoxy-D-manno-octulosonate biosynthesis; 3-deoxy-D-manno-octulosonate from D-ribulose 5-phosphate: step 2/3. HAMAP MF_00056 Bacterial outer membrane biogenesis; lipopolysaccharide biosynthesis. HAMAP MF_00056
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the kdsA family.

Ontologies

Keywords
Biological process	Lipopolysaccharide biosynthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	keto-3-deoxy-D-manno-octulosonic acid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-deoxy-8-phosphooctulonate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 280	280	2-dehydro-3-deoxyphosphooctonate aldolase HAMAP MF_00056		PRO_0000304445

Sequences

	Sequence		Length	Mass (Da)
	Q47Y99-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 7F3F8A1CBE1B30DC	FASTA	280	30,431
	10 20 30 40 50 60 MTIKSVSVKG IDVANEQPFV LFGGMNVLES RDLAMKIAEH YVEVTQKLGI PYVFKASFDK 70 80 90 100 110 120 ANRSSVNSYR GPGLDEGLKI FEEIKSTFNV PIITDVHESY QAQPVSEVVD VIQLPAFLAR 130 140 150 160 170 180 QTDLVVAMAK TGAVINVKKP QFLAAHEMKH IITKFGEAGN ENIILCERGS CYGYNNLVVD 190 200 210 220 230 240 MLAMDEMKNY APVIFDATHA LQKPGGRSDS ADGRRAQAAQ LARSGMAIGI AGLFIEAHPD 250 260 270 280 PSAAKCDGPC ALPLDKLEPY LAQMKALDDL VKGFTPLITD

References

[1]

"The psychrophilic lifestyle as revealed by the genome sequence of Colwellia psychrerythraea 34H through genomic and proteomic analyses." Methe B.A., Nelson K.E., Deming J.W., Momen B., Melamud E., Zhang X., Moult J., Madupu R., Nelson W.C., Dodson R.J., Brinkac L.M., Daugherty S.C., Durkin A.S., DeBoy R.T., Kolonay J.F., Sullivan S.A., Zhou L., Davidsen T.M. , Wu M., Huston A.L., Lewis M., Weaver B., Weidman J.F., Khouri H., Utterback T.R., Feldblyum T.V., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 102:10913-10918(2005) [PubMed: 16043709] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000083 Genomic DNA. Translation: AAZ26459.1.
RefSeq	YP_270221.1.
3D structure databases
SMR	Q47Y99. Positions 1-279.
ModBase	Search...
Protein-protein interaction databases
STRING	Q47Y99.
Genome annotation databases
GeneID	3520710.
GenomeReviews	Gene locus CPS_3547 in contig CP000083_GR.
KEGG	cps:CPS_3547.
NMPDR	fig|167879.3.peg.3312.
TIGR	CPS_3547.
Phylogenomic databases
HOGENOM	Q47Y99.
OMA	VHEPHQA.
Enzyme and pathway databases
BioCyc	CPSY167879:CPS_3547-MON.
Family and domain databases
HAMAP	MF_00056. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR006218. DAHP1/KDSA. IPR006269. KDO8P_synthase. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHER	PTHR21057:SF2. KDO8P_synthase. 1 hit.
Pfam	PF00793. DAHP_synth_1. 1 hit. [Graphical view]
TIGRFAMs	TIGR01362. KDO8P_synth. 1 hit.
ProtoNet	Search...

Entry information

Entry name

KDSA_COLP3

Accession

Primary (citable) accession number: Q47Y99

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	September 13, 2005
Last modified:	November 3, 2009
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)