Reviewed, UniProtKB/Swiss-Prot Q47XX7 (PUR4_COLP3)

Names and origin

Protein names

Recommended name:
    Phosphoribosylformylglycinamidine synthase
      Short name=FGAM synthase
      Short name=FGAMS
    EC=6.3.5.3
Alternative name(s):
    Formylglycinamide ribotide amidotransferase
      Short name=FGARAT
    Formylglycinamide ribotide synthetase

Gene names

Name:	purL
Ordered Locus Names:	CPS_3675

Organism

Colwellia psychrerythraea (strain 34H / ATCC BAA-681) (Vibrio psychroerythus) [Complete proteome] [HAMAP]

Taxonomic identifier

167879 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Colwelliaceae › Colwellia

Protein attributes

Sequence length	1320 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00419
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00419
Subunit structure	Monomer. HAMAP MF_00419
Subcellular location	Cytoplasm. HAMAP MF_00419
Sequence similarities	In the N-terminal section; belongs to the FGAMS family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1320	1320	Phosphoribosylformylglycinamidine synthase HAMAP MF_00419		PRO_0000264571
Regions
	Domain	1067 – 1320	254	Glutamine amidotransferase type-1
	Nucleotide binding	310 – 321	12	ATP Potential
Sites
	Active site	1160	1	Nucleophile By similarity
	Active site	1285	1	By similarity
	Active site	1287	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q47XX7-1 [UniParc]. Last modified December 12, 2006. Version 2. Checksum: B101D5EE17AD23F8	FASTA	1,320	144,259
	10 20 30 40 50 60 MIKNLRGAPA LSDFRVKKLL AQCEQLQLPV NDIYAEFAHF TKLNEELSTS EEKVLQQLLT 70 80 90 100 110 120 YGPTIEEHQP AGLFLLVTPR PGTISPWSSK STDIAHNCGL AKVERLERGI AYYVTLENDA 130 140 150 160 170 180 QLSTSQEAQL NTLLHDRMME SIFNDFAQAS TLFASSEPGE LTAIDIESGG KNALVQANIE 190 200 210 220 230 240 LGLALAEDEV NYLFENFTKL GRNPHDIELY MFAQANSEHC RHKIFNAEWT IDGVKQEKSL 250 260 270 280 290 300 FKMIRNTHEI NPDYVLSAYK DNAAVMVGNK GGRFFPNPET NVYGYNHEDI QILMKVETHN 310 320 330 340 350 360 HPTAISPYPG AATGSGGEIR DEGATGIGSK PKAGLVGFSV SNLRIPDFVQ PWETDFGKPS 370 380 390 400 410 420 RIVTAFDIMI EGPLGGAAFN NEFGRPAILG YFRTYEEEVN SFNGKEVRGY HKPIMLAGGL 430 440 450 460 470 480 GNIRDEHVQK REIIVGANLI ALGGPAMNIG LGGGAASSMA SGQSAESLDF ASVQRENPEM 490 500 510 520 530 540 ERRCQEVIDK CWQLGEENPI AFIHDVGAGG LSNAFPELVA DGGRGGIFEL RNVPNDERSM 550 560 570 580 590 600 APHEIWCNES QERYVIAVSD KNLATFEQIC QRERAPYSVV GRATEEEHLT VTDSHFSDNE 610 620 630 640 650 660 KLNTPIDLPL DVLLGKTPKI YKDVKTATAA GDSLDLSTVT LADAADRILS LPTVAEKTFL 670 680 690 700 710 720 ITIGDRSVTG MVNRDQMVGP WQVPVADCGV TASALDSYHG EAMSLGERTP VALLNFGASA 730 740 750 760 770 780 RLAVAESLTN IAGTDIGDLN RIKLSANWMS PAGHPGEDAG LYEAVKAIGE ELCPALGLTI 790 800 810 820 830 840 PVGKDSMSMK TQWEENGEQK SVTSPLSLVI TAFGVVEDIR KTVTPELRTD KGDTRLVAID 850 860 870 880 890 900 LSKGKKRLGG SCLAQVYKQL GSETPDVDDA EVLKGFFNAM QTLVRAEKVI AYHDISDGGL 910 920 930 940 950 960 FTTVTEMAFA GHTGVDIDIS KLSNGANDDL ATLFNEELGG VIQIRESDVD AIHAILAQHG 970 980 990 1000 1010 1020 ILENCTDIGR LNNEDTIRFS RDGEVVLENS RTYYRTVWAQ TTYRMQSLRD NPECAQQEHD 1030 1040 1050 1060 1070 1080 VKFDTEDPGL NTELTFDINE DIVADLIIRD AVKDAENSAN DITNPRVAIL REQGVNSHVE 1090 1100 1110 1120 1130 1140 MAAAFDRAGF VAIDVHMSDI LSGRADLADF NGLVACGGFS YGDVLGAGEG WAKSILFNAN 1150 1160 1170 1180 1190 1200 ARTMFKTFFE REDTFTLGVC NGCQMLSNLK DIIPGSEHWP HFVQNKSERF EARFSLVEIQ 1210 1220 1230 1240 1250 1260 ESPSVLFKGM EGSRMPIAVS HGEGHAEFSS DAAIDAANNS GTVSMRYVNN YGDVTETYPA 1270 1280 1290 1300 1310 1320 NPNGSVDGIT SLTTTDGRVT IMMPHPERVF RTVANSWHPD SWGEDSPWVR MFRNARAFIG

References

[1]

"The psychrophilic lifestyle as revealed by the genome sequence of Colwellia psychrerythraea 34H through genomic and proteomic analyses." Methe B.A., Nelson K.E., Deming J.W., Momen B., Melamud E., Zhang X., Moult J., Madupu R., Nelson W.C., Dodson R.J., Brinkac L.M., Daugherty S.C., Durkin A.S., DeBoy R.T., Kolonay J.F., Sullivan S.A., Zhou L., Davidsen T.M. , Wu M., Huston A.L., Lewis M., Weaver B., Weidman J.F., Khouri H., Utterback T.R., Feldblyum T.V., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 102:10913-10918(2005) [PubMed: 16043709] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000083 Genomic DNA. Translation: AAZ24314.1.
RefSeq	YP_270343.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q47XX7.
Genome annotation databases
GeneID	3519702.
GenomeReviews	Gene locus CPS_3675 in contig CP000083_GR.
KEGG	cps:CPS_3675.
NMPDR	fig|167879.3.peg.2601.
TIGR	CPS_3675.
Phylogenomic databases
HOGENOM	Q47XX7.
Enzyme and pathway databases
BioCyc	CPSY167879:CPS_3675-MON.
Family and domain databases
HAMAP	MF_00419. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR017926. GATASE_1. IPR010073. PRibForGlyAmidine_synth. [Graphical view]
Pfam	PF00586. AIRS. 1 hit. PF02769. AIRS_C. 2 hits. [Graphical view]
TIGRFAMs	TIGR01735. FGAM_synt. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR4_COLP3

Accession

Primary (citable) accession number: Q47XX7

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	December 12, 2006
Last modified:	September 1, 2009
This is version 29 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)