Reviewed, UniProtKB/Swiss-Prot Q47XB6 (HIS7_COLP3)

Names and origin

Protein names

Recommended name:
    Histidine biosynthesis bifunctional protein hisB
Including the following 2 domains:
    1- Recommended name:
            Histidinol-phosphatase
              EC=3.1.3.15
    2- Recommended name:
            Imidazoleglycerol-phosphate dehydratase
                Short name=IGPD
              EC=4.2.1.19

Gene names

Name:	hisB
Ordered Locus Names:	CPS_3892

Organism

Colwellia psychrerythraea (strain 34H / ATCC BAA-681) (Vibrio psychroerythus) [Complete proteome] [HAMAP]

Taxonomic identifier

167879 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Colwelliaceae › Colwellia

Protein attributes

Sequence length	361 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + H2O. HAMAP MF_01022 L-histidinol phosphate + H2O = L-histidinol + phosphate. HAMAP MF_01022
Pathway	Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 6/9. HAMAP MF_01022 Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 8/9.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	In the N-terminal section; belongs to the histidinol-phosphatase family. In the C-terminal section; belongs to the imidazoleglycerol-phosphate dehydratase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Histidine biosynthesis
Cellular component	Cytoplasm
Molecular function	Hydrolase Lyase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	histidine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	histidinol-phosphatase activity Inferred from electronic annotation. Source: HAMAP imidazoleglycerol-phosphate dehydratase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 361	361	Histidine biosynthesis bifunctional protein hisB HAMAP MF_01022		PRO_0000319738
Regions
	Region	1 – 172	172	Histidinol-phosphatase HAMAP MF_01022
	Region	173 – 361	189	Imidazoleglycerol-phosphate dehydratase HAMAP MF_01022

Sequences

	Sequence		Length	Mass (Da)
	Q47XB6-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 0DAD638A63B8A9F1	FASTA	361	39,901
	10 20 30 40 50 60 MSNTQEKILF IDRDGTLVEE PAIDKQLDTL EKLVFEPNVI AELLKLQAKG FKLVMVSNQD 70 80 90 100 110 120 GLGTNSFPQA DFDLPHNKMM DLFSSQGVHF QDVLLCPHFD EDNCNCRKPK LGLVSEYLQQ 130 140 150 160 170 180 GRVDFANSFV IGDRETDMGL AANMGIVGIK YDPETLNWAQ VSEQIITQLE QPRIATVTRT 190 200 210 220 230 240 TKETDITVTV NLDKAGESSI DTGLGFFDHM LDQISTHGGF SLQCHVSGDY HIDEHHSVED 250 260 270 280 290 300 TALALGQALK QALGNKRGIN RFGFTIPMDE CRAECAIDLS GRPWLEFDAD FTSANVGTMS 310 320 330 340 350 360 TQMVPHFFRS LADSMLITLH LSTSKGNCHH QVESLFKVFG RALGQAIKVD GDAMPSSKGT L

References

[1]

"The psychrophilic lifestyle as revealed by the genome sequence of Colwellia psychrerythraea 34H through genomic and proteomic analyses." Methe B.A., Nelson K.E., Deming J.W., Momen B., Melamud E., Zhang X., Moult J., Madupu R., Nelson W.C., Dodson R.J., Brinkac L.M., Daugherty S.C., Durkin A.S., DeBoy R.T., Kolonay J.F., Sullivan S.A., Zhou L., Davidsen T.M. , Wu M., Huston A.L., Lewis M., Weaver B., Weidman J.F., Khouri H., Utterback T.R., Feldblyum T.V., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 102:10913-10918(2005) [PubMed: 16043709] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000083 Genomic DNA. Translation: AAZ28659.1.
RefSeq	YP_270554.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3522920.
GenomeReviews	Gene locus CPS_3892 in contig CP000083_GR.
KEGG	cps:CPS_3892.
NMPDR	fig|167879.3.peg.3532.
TIGR	CPS_3892.
Phylogenomic databases
HOGENOM	Q47XB6.
OMA	Q47XB6. MVSNQDG.
Enzyme and pathway databases
BioCyc	CPSY167879:CPS_3892-MON.
Family and domain databases
HAMAP	MF_01022. [Tree]
InterPro	IPR006549. HAD-SF_hydro_IIIA. IPR005954. HisB_N. IPR006543. Histidinol-phos. IPR000807. Imidazole_glycer-P_deHydtase. IPR013954. PNK3P_central-region. [Graphical view]
PANTHER	PTHR23133:SF2. Imidazole-GPD. 1 hit.
Pfam	PF00475. IGPD. 1 hit. PF08645. PNK3P. 1 hit. [Graphical view]
ProDom	PD002282. IGPD. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01662. HAD-SF-IIIA. 1 hit. TIGR01261. hisB_Nterm. 1 hit. TIGR01656. Histidinol-ppas. 1 hit.
PROSITE	PS00954. IGP_DEHYDRATASE_1. 1 hit. PS00955. IGP_DEHYDRATASE_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HIS7_COLP3

Accession

Primary (citable) accession number: Q47XB6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	September 13, 2005
Last modified:	June 16, 2009
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)