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Protein

Acetyl-coenzyme A synthetase

Gene

acs

Organism
Colwellia psychrerythraea (strain 34H / ATCC BAA-681) (Vibrio psychroerythus)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. AcsA undergoes a two-step reaction. In the first half reaction, AcsA combines acetate with ATP to form acetyl-adenylate (AcAMP) intermediate. In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA.UniRule annotation

Catalytic activityi

ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei309Coenzyme AUniRule annotation1
Binding sitei498ATPUniRule annotation1
Binding sitei513ATPUniRule annotation1
Binding sitei521Coenzyme A; via carbonyl oxygenUniRule annotation1
Binding sitei524ATPUniRule annotation1
Metal bindingi535Magnesium; via carbonyl oxygenUniRule annotation1
Metal bindingi537Magnesium; via carbonyl oxygenUniRule annotation1
Metal bindingi540Magnesium; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi385 – 387ATPUniRule annotation3
Nucleotide bindingi409 – 414ATPUniRule annotation6

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

LigaseUniRule annotationImported

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Acetyl-coenzyme A synthetaseUniRule annotation (EC:6.2.1.1UniRule annotation)
Short name:
AcCoA synthetaseUniRule annotation
Short name:
AcsUniRule annotation
Alternative name(s):
Acetate--CoA ligaseUniRule annotation
Acyl-activating enzymeUniRule annotation
Gene namesi
Name:acsImported
Synonyms:acsAUniRule annotation
Ordered Locus Names:CPS_3955Imported
OrganismiColwellia psychrerythraea (strain 34H / ATCC BAA-681) (Vibrio psychroerythus)Imported
Taxonomic identifieri167879 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesColwelliaceaeColwellia
Proteomesi
  • UP000000547 Componenti: Chromosome

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei607N6-acetyllysineUniRule annotation1

Post-translational modificationi

Acetylated. Deacetylation by the SIR2-homolog deacetylase activates the enzyme.UniRule annotation

Keywords - PTMi

AcetylationUniRule annotation

Interactioni

Protein-protein interaction databases

STRINGi167879.CPS_3955.

Structurei

3D structure databases

ProteinModelPortaliQ47X57.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini25 – 81ACAS_NInterPro annotationAdd BLAST57
Domaini84 – 520AMP-bindingInterPro annotationAdd BLAST437
Domaini529 – 607AMP-binding_CInterPro annotationAdd BLAST79

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni191 – 194Coenzyme A bindingUniRule annotation4

Sequence similaritiesi

Belongs to the ATP-dependent AMP-binding enzyme family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108IQF. Bacteria.
COG0365. LUCA.
HOGENOMiHOG000229981.
KOiK01895.
OMAiIKNMCEY.
OrthoDBiPOG091H059D.

Family and domain databases

CDDicd05966. ACS. 1 hit.
HAMAPiMF_01123. Ac_CoA_synth. 1 hit.
InterProiIPR011904. Ac_CoA_lig.
IPR032387. ACAS_N.
IPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamiPF16177. ACAS_N. 1 hit.
PF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02188. Ac_CoA_lig_AcsA. 1 hit.
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q47X57-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKELKSCYPV SEKAKAHTHI DAATYDAMYK KSIEQPDEFW GEQAKEFIDW
60 70 80 90 100
YKPWDSVSKV DLKNSEINWF SGGKLNVSYN CIDRHLATKA NDTAIIFEGD
110 120 130 140 150
DPNDDSKVTY QELHDHVCRF ANLLKERGVK KGDRVCIYMP MIPEVGYAML
160 170 180 190 200
ACARIGAIHS VVFGGFSTES IKARVLDADC KVVITADQSL RGGKRIPLKS
210 220 230 240 250
NVDAAVIDCP NVHSVIVVAR TGGDVAWNDK VDINYEEAVA KQSAICEPEV
260 270 280 290 300
MDAEDPLFVL YTSGSTGTPK GVVHTCGGYI LYAAMTHKYV FDYKEGEVYW
310 320 330 340 350
CTADAGWITG HSYIFYGPLA NGATTLVFEG VPTYPDAGRF WQVCEKHKVN
360 370 380 390 400
VFYTAPTAIR ALMSIGDDLV NQADLSSLRL LGTVGEPINP EAWHWYYEVV
410 420 430 440 450
GKSNCPIVDT WWQTETGGIL ITSLPGAVDM KPGCAGKPFF GVQPALFDKD
460 470 480 490 500
GNTLEGENAG LLVMTASWPG QLRTVYGDHN RFYQTYLGQY PGNYFTGDGA
510 520 530 540 550
KRDEDGYYWI TGRVDDVLNV SGHRLGTAEI ESALVLHPAV AEAAVVGYPH
560 570 580 590 600
EIKGQGVYCY VTLMGNATES DELNVELREF VAKELGRFAK PDYIQWSPGL
610 620 630 640
PKTRSGKIMR RILRKIAEND VDSLGDTSTL ADPSVVENLI DKRIIHGA
Length:648
Mass (Da):71,614
Last modified:September 13, 2005 - v1
Checksum:i12B79CF080B776AB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000083 Genomic DNA. Translation: AAZ26507.1.
RefSeqiWP_011044699.1. NC_003910.7.

Genome annotation databases

EnsemblBacteriaiAAZ26507; AAZ26507; CPS_3955.
KEGGicps:CPS_3955.
PATRICi21470797. VBIColPsy94388_3583.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000083 Genomic DNA. Translation: AAZ26507.1.
RefSeqiWP_011044699.1. NC_003910.7.

3D structure databases

ProteinModelPortaliQ47X57.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi167879.CPS_3955.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAZ26507; AAZ26507; CPS_3955.
KEGGicps:CPS_3955.
PATRICi21470797. VBIColPsy94388_3583.

Phylogenomic databases

eggNOGiENOG4108IQF. Bacteria.
COG0365. LUCA.
HOGENOMiHOG000229981.
KOiK01895.
OMAiIKNMCEY.
OrthoDBiPOG091H059D.

Family and domain databases

CDDicd05966. ACS. 1 hit.
HAMAPiMF_01123. Ac_CoA_synth. 1 hit.
InterProiIPR011904. Ac_CoA_lig.
IPR032387. ACAS_N.
IPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamiPF16177. ACAS_N. 1 hit.
PF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02188. Ac_CoA_lig_AcsA. 1 hit.
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiQ47X57_COLP3
AccessioniPrimary (citable) accession number: Q47X57
Entry historyi
Integrated into UniProtKB/TrEMBL: September 13, 2005
Last sequence update: September 13, 2005
Last modified: November 2, 2016
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.