Reviewed, UniProtKB/Swiss-Prot Q47WB0 (MAO1_COLP3)

Names and origin

Protein names

Recommended name:
    NAD-dependent malic enzyme
      Short name=NAD-ME
    EC=1.1.1.38

Gene names

Name:	sfcA
Ordered Locus Names:	CPS_4262

Organism

Colwellia psychrerythraea (strain 34H / ATCC BAA-681) (Vibrio psychroerythus) [Complete proteome] [HAMAP]

Taxonomic identifier

167879 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Colwelliaceae › Colwellia

Protein attributes

Sequence length	562 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-malate + NAD+ = pyruvate + CO2 + NADH. HAMAP MF_01619
Cofactor	Divalent metal cations. Prefers magnesium or manganese By similarity. HAMAP MF_01619
Subunit structure	Homotetramer By similarity. HAMAP MF_01619
Sequence similarities	Belongs to the malic enzymes family.

Ontologies

Keywords
Ligand	Metal-binding NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	malate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro malate dehydrogenase (oxaloacetate-decarboxylating) activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 562	562	NAD-dependent malic enzyme HAMAP MF_01619		PRO_0000160214
Sites
	Active site	101	1	Proton donor By similarity
	Active site	172	1	Proton acceptor By similarity
	Metal binding	243	1	Divalent metal cation By similarity
	Metal binding	244	1	Divalent metal cation By similarity
	Metal binding	267	1	Divalent metal cation By similarity
	Binding site	154	1	NAD By similarity
	Binding site	267	1	NAD By similarity
	Binding site	415	1	NAD By similarity
	Site	267	1	Important for activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q47WB0-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 2A8446B4882AD4A8	FASTA	562	62,024
	10 20 30 40 50 60 MPQSQRPLYI PYAGPSLLET PLLNKGSAFS KEERGSFNLT GLLPPRFESI DEQAERAFRQ 70 80 90 100 110 120 YSCFQTNINK HIYLRAIHDN NETLFFKLVQ NNLAEMMPII YTPTVGDACE QFSDIYRSSR 130 140 150 160 170 180 GLFISYEDRF NIDDMLRNAT KNKVKVIVVT DGERILGLGD QGIGGMGIPI GKLSLYTACG 190 200 210 220 230 240 GISPAHTLPV MLDVGTNNQK LLDDPMYMGA RHKRIDQDSY DEFLELFISA VKRRWPNVLL 250 260 270 280 290 300 QFEDFAQPNA MPLLQRYKDR ICCFNDDIQG TASVTVGTLL AACRSKGSKL SELNVAFVGA 310 320 330 340 350 360 GSAGCGIAEQ IISQMMNEGL GAEQARSQVF MVDRFGLLTQ GMGELRDFQQ KLVQSNEAIA 370 380 390 400 410 420 EWDIAGEFAS LVEVMHGAKP DILIGVSGQA GLFTEKVITA MKSHCEMPII FPLSNPSRQV 430 440 450 460 470 480 EATPSQVINW TQGQVIIATG SPFDPIEYQG KTFPIAQCNN SYIFPGVGLA VVAANISRIT 490 500 510 520 530 540 DGMLQVASET LAAASPLANG ESDELLPPLT SIAQLSRDIA FAIAKVAYKQ GLALELTDDE 550 560 LLAKIEHNFW KPEYRQYRRT SL

References

[1]

"The psychrophilic lifestyle as revealed by the genome sequence of Colwellia psychrerythraea 34H through genomic and proteomic analyses." Methe B.A., Nelson K.E., Deming J.W., Momen B., Melamud E., Zhang X., Moult J., Madupu R., Nelson W.C., Dodson R.J., Brinkac L.M., Daugherty S.C., Durkin A.S., DeBoy R.T., Kolonay J.F., Sullivan S.A., Zhou L., Davidsen T.M. , Wu M., Huston A.L., Lewis M., Weaver B., Weidman J.F., Khouri H., Utterback T.R., Feldblyum T.V., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 102:10913-10918(2005) [PubMed: 16043709] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000083 Genomic DNA. Translation: AAZ25596.1.
RefSeq	YP_270912.1.
3D structure databases
SMR	Q47WB0. Positions 13-561.
ModBase	Search...
Protein-protein interaction databases
STRING	Q47WB0.
Genome annotation databases
GeneID	3519838.
GenomeReviews	Gene locus CPS_4262 in contig CP000083_GR.
KEGG	cps:CPS_4262.
NMPDR	fig|167879.3.peg.3841.
TIGR	CPS_4262.
Phylogenomic databases
eggNOG	COG0281.
HOGENOM	HBG289821.
OMA	IQDNNET.
Enzyme and pathway databases
BioCyc	CPSY167879:CPS_4262-MONOMER.
Family and domain databases
HAMAP	MF_01619. NAD_malic_enz. [Tree]
InterPro	IPR015884. Malic_enzyme_CS. IPR012301. Malic_N. IPR012302. Malic_NAD_bd. IPR001891. Malic_OxRdtase. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00390. malic. 1 hit. PF03949. Malic_M. 1 hit. [Graphical view]
PRINTS	PR00072. MALOXRDTASE.
PROSITE	PS00331. MALIC_ENZYMES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MAO1_COLP3

Accession

Primary (citable) accession number: Q47WB0

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	September 13, 2005
Last modified:	January 19, 2010
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)