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Q47VR1 (DDL_COLP3) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:CPS_4463
OrganismColwellia psychrerythraea (strain 34H / ATCC BAA-681) (Vibrio psychroerythus) [Complete proteome] [HAMAP]
Taxonomic identifier167879 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesColwelliaceaeColwellia

Protein attributes

Sequence length317 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 317317D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_0000341084

Regions

Domain113 – 312200ATP-grasp
Nucleotide binding143 – 19856ATP By similarity

Sites

Metal binding2661Magnesium or manganese 1 By similarity
Metal binding2791Magnesium or manganese 1 By similarity
Metal binding2791Magnesium or manganese 2 By similarity
Metal binding2811Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q47VR1 [UniParc].

Last modified September 13, 2005. Version 1.
Checksum: BD2E2B364169E9B9

FASTA31734,454
        10         20         30         40         50         60 
MVKALDRSLA LLKQEKIAVL YGGNSAEREV SLNSGQAIAK GLEDAGFNVV LIDTKVVPLT 

        70         80         90        100        110        120 
DLVSNKIDRV FIALHGRGGE DGCLQGALEY LDIPYTGSNV LGSSLSMDKV RSKQIFKACG 

       130        140        150        160        170        180 
IPTAPFTVVN KAEFSTLSLE NILADLGGRV MVKPANEGSS IGMAQAKTIA QLRNALIEAF 

       190        200        210        220        230        240 
GFDTQVLLEA WIDGPEYTVT ILGDKALPAI HMETPREFYD YEAKYQSTST QYHCPCGLNE 

       250        260        270        280        290        300 
RDENEIKSLS MKAFNATGAQ GWGRVDIMRN KTGEWQILEV NTVPGMTETS LVPKAAKVFG 

       310 
LNFSELVTQI LQLSVKE 

« Hide

References

[1]"The psychrophilic lifestyle as revealed by the genome sequence of Colwellia psychrerythraea 34H through genomic and proteomic analyses."
Methe B.A., Nelson K.E., Deming J.W., Momen B., Melamud E., Zhang X., Moult J., Madupu R., Nelson W.C., Dodson R.J., Brinkac L.M., Daugherty S.C., Durkin A.S., DeBoy R.T., Kolonay J.F., Sullivan S.A., Zhou L., Davidsen T.M. expand/collapse author list , Wu M., Huston A.L., Lewis M., Weaver B., Weidman J.F., Khouri H., Utterback T.R., Feldblyum T.V., Fraser C.M.
Proc. Natl. Acad. Sci. U.S.A. 102:10913-10918(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 34H / ATCC BAA-681.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000083 Genomic DNA. Translation: AAZ27413.1.
RefSeqYP_271111.1. NC_003910.7.

3D structure databases

ProteinModelPortalQ47VR1.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING167879.CPS_4463.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAZ27413; AAZ27413; CPS_4463.
GeneID3521671.
KEGGcps:CPS_4463.
PATRIC21471759. VBIColPsy94388_4060.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011592.
KOK01921.
OMAEDPARES.
OrthoDBEOG6ND0KB.

Enzyme and pathway databases

BioCycCPSY167879:GI48-4464-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 2 hits.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_COLP3
AccessionPrimary (citable) accession number: Q47VR1
Entry history
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: September 13, 2005
Last modified: May 14, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways