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Q47VJ9 (PDXA_COLP3) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 57. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
4-hydroxythreonine-4-phosphate dehydrogenase

EC=1.1.1.262
Alternative name(s):
4-(phosphohydroxy)-L-threonine dehydrogenase
Gene names
Name:pdxA
Ordered Locus Names:CPS_4525
OrganismColwellia psychrerythraea (strain 34H / ATCC BAA-681) (Vibrio psychroerythus) [Complete proteome] [HAMAP]
Taxonomic identifier167879 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesColwelliaceaeColwellia

Protein attributes

Sequence length326 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the NAD(P)-dependent oxidation of 4-(phosphohydroxy)-L-threonine (HTP) into 2-amino-3-oxo-4-(phosphohydroxy)butyric acid which spontaneously decarboxylates to form 3-amino-2-oxopropyl phosphate (AHAP) By similarity. HAMAP-Rule MF_00536

Catalytic activity

4-phosphonooxy-L-threonine + NAD+ = 3-amino-2-oxopropyl phosphate + CO2 + NADH. HAMAP-Rule MF_00536

Cofactor

Binds 1 divalent metal cation per subunit. Can use ions such as zinc, magnesium or cobalt By similarity.

Pathway

Cofactor biosynthesis; pyridoxine 5'-phosphate biosynthesis; pyridoxine 5'-phosphate from D-erythrose 4-phosphate: step 4/5. HAMAP-Rule MF_00536

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00536

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00536.

Miscellaneous

The active site is located at the dimer interface By similarity.

Sequence similarities

Belongs to the PdxA family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 3263264-hydroxythreonine-4-phosphate dehydrogenase HAMAP-Rule MF_00536
PRO_1000128239

Sites

Metal binding1641Divalent metal cation; shared with dimeric partner By similarity
Metal binding2091Divalent metal cation; shared with dimeric partner By similarity
Metal binding2641Divalent metal cation; shared with dimeric partner By similarity
Binding site1341Substrate By similarity
Binding site1351Substrate By similarity
Binding site2721Substrate By similarity
Binding site2811Substrate By similarity
Binding site2901Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q47VJ9 [UniParc].

Last modified September 13, 2005. Version 1.
Checksum: C7BD6E284AC6B7DB

FASTA32634,947
        10         20         30         40         50         60 
MTQRIAITPG EPAGVGPDLI ITIAQQDWPV EMVVIASKAL LQERSKALSL PLTIIDYDQH 

        70         80         90        100        110        120 
APAKSQKSGS LTVLDVELTE PCVPGTLNSA NGSYVVETLR IASEKNISGE FDAIVTGPVH 

       130        140        150        160        170        180 
KGLINKAGIA FSGHTEYFAT QANCSDVVMM LATKGLRVAL VTTHIPLAYV SKAITYERLQ 

       190        200        210        220        230        240 
KVTRILHKDL QEKFGIKSPK IYACGINPHA GEDGHLGREE IEIMEPAFAE LRADGIDIIG 

       250        260        270        280        290        300 
PLPADTIFQE KYLAEADAIL AMYHDQGLPV LKYKGFGSSV NITLGLPFIR TSVDHGTALE 

       310        320 
LAGKGTADSG SFIEAMNNAI NLASNK 

« Hide

References

[1]"The psychrophilic lifestyle as revealed by the genome sequence of Colwellia psychrerythraea 34H through genomic and proteomic analyses."
Methe B.A., Nelson K.E., Deming J.W., Momen B., Melamud E., Zhang X., Moult J., Madupu R., Nelson W.C., Dodson R.J., Brinkac L.M., Daugherty S.C., Durkin A.S., DeBoy R.T., Kolonay J.F., Sullivan S.A., Zhou L., Davidsen T.M. expand/collapse author list , Wu M., Huston A.L., Lewis M., Weaver B., Weidman J.F., Khouri H., Utterback T.R., Feldblyum T.V., Fraser C.M.
Proc. Natl. Acad. Sci. U.S.A. 102:10913-10918(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 34H / ATCC BAA-681.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000083 Genomic DNA. Translation: AAZ28186.1.
RefSeqYP_271173.1. NC_003910.7.

3D structure databases

ProteinModelPortalQ47VJ9.
SMRQ47VJ9. Positions 2-325.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING167879.CPS_4525.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAZ28186; AAZ28186; CPS_4525.
GeneID3522445.
KEGGcps:CPS_4525.
PATRIC21471875. VBIColPsy94388_4118.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1995.
HOGENOMHOG000221592.
KOK00097.
OMADTLFQDK.
OrthoDBEOG6GN6ZC.

Enzyme and pathway databases

BioCycCPSY167879:GI48-4526-MONOMER.
UniPathwayUPA00244; UER00312.

Family and domain databases

Gene3D3.40.718.10. 1 hit.
HAMAPMF_00536. PdxA.
InterProIPR024084. IsoPropMal-DH-like_dom.
IPR005255. PdxA.
[Graphical view]
PfamPF04166. PdxA. 1 hit.
[Graphical view]
TIGRFAMsTIGR00557. pdxA. 1 hit.
ProtoNetSearch...

Entry information

Entry namePDXA_COLP3
AccessionPrimary (citable) accession number: Q47VJ9
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: September 13, 2005
Last modified: February 19, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways