Q47VD0 (PCKA_COLP3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoenolpyruvate carboxykinase [ATP]
Short name=PEP carboxykinase
Short name=PEPCK
EC=4.1.1.49

Alternative name(s):
Phosphoenolpyruvate carboxylase

Gene names

Name:	pckA
Ordered Locus Names:	CPS_4595

Organism

Colwellia psychrerythraea (strain 34H / ATCC BAA-681) (Vibrio psychroerythus) [Complete proteome] [HAMAP]

Taxonomic identifier

167879 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Colwelliaceae › Colwellia

Protein attributes

Sequence length	536 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + oxaloacetate = ADP + phosphoenolpyruvate + CO2. HAMAP MF_00453
Pathway	Carbohydrate biosynthesis; gluconeogenesis. HAMAP MF_00453
Subcellular location	Cytoplasm By similarity HAMAP MF_00453.
Sequence similarities	Belongs to the phosphoenolpyruvate carboxykinase [ATP] family.

Ontologies

Keywords
Biological process	Gluconeogenesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Decarboxylase Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	gluconeogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoenolpyruvate carboxykinase (ATP) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 536	536	Phosphoenolpyruvate carboxykinase [ATP] HAMAP MF_00453		PRO_0000236922
Regions
	Nucleotide binding	244 – 251	8	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q47VD0 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: B1B699E4134D59D6	FASTA	536	59,306
	10 20 30 40 50 60 MTAPKNSIDL SQYGIDDVNE VVYNPSYELL FSEETKAGLE GFDKGIVTEL GAVNVDTGIF 70 80 90 100 110 120 TGRSPKDKYI VRDDVTRDTV WWSDQGKNDN KAMTPETWDH LKGLVTTQLS GQRLFVVDTF 130 140 150 160 170 180 CGADEATRLK VRFITQVAWQ AHFVKNMFIR PTDAELENYE PDFVVMNGAK TVNDKWEEQG 190 200 210 220 230 240 LNSENFVAFN LTEKIQLIGG TWYGGEMKKG MFSMMNYYLP LQGIASMHCS ANVGEDGDTA 250 260 270 280 290 300 IFFGLSGTGK TTLSTDPKRQ LIGDDEHGWD DNGVFNFEGG CYAKTINLSK ENEPDIYNAI 310 320 330 340 350 360 RRDALLENVT VDANGKIDFD DNSKTENTRV SYPIHHIDNI VKPVSRAGHA KKVIFLTADA 370 380 390 400 410 420 FGVLPPVAKL TPEQTEYYFL SGFTAKLAGT ERGITEPTPT FSSCFGAAFL SLHPTQYAEV 430 440 450 460 470 480 LHKRMDDAGA EAYLVNTGWN GTGKRISIKA TRAIIDAILD GSIDNAETET VPFFNLEVPK 490 500 510 520 530 VIAGVEGDIL DPRNTYEDPS VWNDKAVDLA KRFVNNFDKF TDTDNGKALV AAGPQL

References

[1]

"The psychrophilic lifestyle as revealed by the genome sequence of Colwellia psychrerythraea 34H through genomic and proteomic analyses." Methe B.A., Nelson K.E., Deming J.W., Momen B., Melamud E., Zhang X., Moult J., Madupu R., Nelson W.C., Dodson R.J., Brinkac L.M., Daugherty S.C., Durkin A.S., DeBoy R.T., Kolonay J.F., Sullivan S.A., Zhou L., Davidsen T.M. , Wu M., Huston A.L., Lewis M., Weaver B., Weidman J.F., Khouri H., Utterback T.R., Feldblyum T.V., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 102:10913-10918(2005) [PubMed: 16043709] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 34H / ATCC BAA-681.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000083 Genomic DNA. Translation: AAZ28633.1.
RefSeq	YP_271242.1. NC_003910.7.
3D structure databases
ProteinModelPortal	Q47VD0.
SMR	Q47VD0. Positions 9-536.
ModBase	Search...
Protein-protein interaction databases
STRING	Q47VD0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3522894.
GenomeReviews	Gene locus CPS_4595 in contig CP000083_GR.
KEGG	cps:CPS_4595.
NMPDR	fig|167879.3.peg.4457.
PATRIC	21472013. VBIColPsy94388_4187.
TIGR	CPS_4595.
Phylogenomic databases
eggNOG	COG1866.
HOGENOM	HBG293387.
OMA	VYNPSYE.
ProtClustDB	PRK09344.
Enzyme and pathway databases
BioCyc	CPSY167879:CPS_4595-MONOMER.
Family and domain databases
HAMAP	MF_00453. PEPCK_ATP. [Tree]
InterPro	IPR001272. PEP_carboxykinase_ATP. IPR013035. PEP_carboxykinase_C. IPR008210. PEP_carboxykinase_N. IPR015994. PEPCK_ATP_CS. [Graphical view]
Gene3D	G3DSA:3.90.228.20. PEP_carboxykinase_C. 2 hits. G3DSA:3.40.449.10. PEP_carboxykinase_N. 1 hit.
KO	K01610.
Pfam	PF01293. PEPCK_ATP. 1 hit. [Graphical view]
PIRSF	PIRSF006294. PEP_crbxkin. 1 hit.
SUPFAM	SSF68923. PEP_carboxykinase_N. 1 hit.
TIGRFAMs	TIGR00224. PckA. 1 hit.
PROSITE	PS00532. PEPCK_ATP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PCKA_COLP3

Accession

Primary (citable) accession number: Q47VD0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	September 13, 2005
Last modified:	January 25, 2012
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program