Reviewed, UniProtKB/Swiss-Prot Q47UW8 (CYSI_COLP3)

Names and origin

Protein names

Recommended name:
    Sulfite reductase [NADPH] hemoprotein beta-component
      Short name=SiR-HP
      Short name=SiRHP
    EC=1.8.1.2

Gene names

Name:	cysI
Ordered Locus Names:	CPS_4760

Organism

Colwellia psychrerythraea (strain 34H / ATCC BAA-681) (Vibrio psychroerythus) [Complete proteome] [HAMAP]

Taxonomic identifier

167879 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Colwelliaceae › Colwellia

Protein attributes

Sequence length	584 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate By similarity. HAMAP MF_01540
Catalytic activity	H2S + 3 NADP+ + 3 H2O = sulfite + 3 NADPH. HAMAP MF_01540
Cofactor	Binds 1 siroheme per subunit By similarity. HAMAP MF_01540 Binds 1 4Fe-4S cluster per subunit By similarity. HAMAP MF_01540
Pathway	Sulfur metabolism; hydrogen sulfide biosynthesis; hydrogen sulfide from sulfite (NADPH route): step 1/1. HAMAP MF_01540
Subunit structure	Alpha(8)-beta8. The alpha component is a flavoprotein, the beta component is a hemoprotein By similarity. HAMAP MF_01540
Sequence similarities	Belongs to the nitrite and sulfite reductase 4Fe-4S domain family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Cysteine biosynthesis
Ligand	4Fe-4S Heme Iron Iron-sulfur Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW hydrogen sulfide biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW sulfate assimilation Inferred from electronic annotation. Source: HAMAP
Cellular component	sulfite reductase complex (NADPH) Inferred from electronic annotation. Source: InterPro
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NADP or NADPH binding Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro sulfite reductase (NADPH) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 584	584	Sulfite reductase [NADPH] hemoprotein beta-component HAMAP MF_01540		PRO_0000292960
Sites
	Metal binding	447	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	453	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	492	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	496	1	Iron (siroheme axial ligand) By similarity
	Metal binding	496	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q47UW8-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 136505C82F1E25E8	FASTA	584	64,788
	10 20 30 40 50 60 MSNNFTKVEN PVLIVEGKQA DNERLKAESD YLRGTIKDDL QDRMTGGFTS DNFQLIRTHG 70 80 90 100 110 120 MYQQDDRDIR AERQKQKLEP LHNVMLRARL PGGIINPTQW LAIDKFADDY TSYGSIRLTT 130 140 150 160 170 180 RQTFQFHGVL KPNIKLMHQT LNSVGLDSIA TAGDVNRNVL CTSNPVESAL HQEAYEWATK 190 200 210 220 230 240 ISEHLLPKTR AYAEIWLDEE KVETTEADEI EPILGSNYLP RKFKTTVVIP PNNDIDVHAN 250 260 270 280 290 300 DLNFVAISEG GELIGFNVLV GGGLAMTHGD KATYPRCADD FGFIPKEHTL AIAAAVVTTQ 310 320 330 340 350 360 RDWGNRVNRK NAKTKYTLDR VGVDTFKAEV ERRAGIKFSE SRSYEFTHRG DSFGWVEGID 370 380 390 400 410 420 GKNHLTLFIE NGRILDFNGA NSDSKALKTG MREIAKIHKG DFRLTANQNL IVAGVSAEDK 430 440 450 460 470 480 TIIEQLAREH GLINDGVSNQ RKSSMACVAF PTCPLAMAEA ERYLPGLVDD VEAILEKNGL 490 500 510 520 530 540 KDDSIILRVT GCPNGCGRAM LAEIGLVGKG PGKYNMYLGS DLAGSRVPKL YKENVDEAGV 550 560 570 580 LSEIDALSAR WSAERNDGEA FGDFVIRAGI VEQVIVSFRD FHHA

References

[1]

"The psychrophilic lifestyle as revealed by the genome sequence of Colwellia psychrerythraea 34H through genomic and proteomic analyses." Methe B.A., Nelson K.E., Deming J.W., Momen B., Melamud E., Zhang X., Moult J., Madupu R., Nelson W.C., Dodson R.J., Brinkac L.M., Daugherty S.C., Durkin A.S., DeBoy R.T., Kolonay J.F., Sullivan S.A., Zhou L., Davidsen T.M. , Wu M., Huston A.L., Lewis M., Weaver B., Weidman J.F., Khouri H., Utterback T.R., Feldblyum T.V., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 102:10913-10918(2005) [PubMed: 16043709] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000083 Genomic DNA. Translation: AAZ25063.1.
RefSeq	YP_271404.1.
3D structure databases
SMR	Q47UW8. Positions 85-581.
ModBase	Search...
Protein-protein interaction databases
STRING	Q47UW8.
Genome annotation databases
GeneID	3518522.
GenomeReviews	Gene locus CPS_4760 in contig CP000083_GR.
KEGG	cps:CPS_4760.
NMPDR	fig|167879.3.peg.4622.
TIGR	CPS_4760.
Phylogenomic databases
eggNOG	COG0155.
HOGENOM	HBG626671.
OMA	PGDNSVD.
Enzyme and pathway databases
BioCyc	CPSY167879:CPS_4760-MONOMER.
Family and domain databases
HAMAP	MF_01540. CysI. [Tree]
InterPro	IPR011786. CysI. IPR005117. NiRdtase/SiRdtase_haem-b_fer. IPR006067. NO2/SO3_Rdtase_4Fe4S_dom. IPR006066. NO2/SO3_Rdtase_FeS/sirohaem_BS. [Graphical view]
Pfam	PF01077. NIR_SIR. 1 hit. PF03460. NIR_SIR_ferr. 2 hits. [Graphical view]
PRINTS	PR00397. SIROHAEM.
TIGRFAMs	TIGR02041. CysI. 1 hit.
PROSITE	PS00365. NIR_SIR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYSI_COLP3

Accession

Primary (citable) accession number: Q47UW8

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 26, 2007
Last sequence update:	September 13, 2005
Last modified:	February 9, 2010
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)