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Reviewed, UniProtKB/Swiss-Prot Q47UW8 (CYSI_COLP3)

Last modified November 25, 2008. Version 20. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Sulfite reductase [NADPH] hemoprotein beta-component
      Short name=SIR-HP
      Short name=SIRHP
    EC=1.8.1.2
Gene names
Name: cysI
Ordered Locus Names: CPS_4760
OrganismColwellia psychrerythraea (strain 34H / ATCC BAA-681) (Vibrio psychroerythus) [Complete proteome] [HAMAP]
Taxonomic identifier167879 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesColwelliaceaeColwellia

Protein attributes

Sequence length584 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

General annotation (Comments)

Function

This enzyme catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate By similarity.

Catalytic activity

H(2)S + 3 NADP(+) + 3 H(2)O = sulfite + 3 NADPH. HAMAP MF_01540

Cofactor

Binds 1 siroheme per subunit By similarity.

Binds 1 4Fe-4S cluster per subunit By similarity.

Subunit structure

Alpha(8)-beta(4). The alpha component is a flavoprotein, the beta component is a hemoprotein By similarity.

Sequence similarities

Belongs to the nitrite and sulfite reductase 4Fe-4S domain family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 584584Sulfite reductase [NADPH] hemoprotein beta-component HAMAP MF_01540
PRO_0000292960

Sites

Metal binding4471Iron-sulfur (4Fe-4S) By similarity
Metal binding4531Iron-sulfur (4Fe-4S) By similarity
Metal binding4921Iron-sulfur (4Fe-4S) By similarity
Metal binding4961Iron (siroheme axial ligand) By similarity
Metal binding4961Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q47UW8-1 [UniParc].

Last modified September 13, 2005. Version 1.
Checksum: 136505C82F1E25E8

FASTA58464,788
        10         20         30         40         50         60 
MSNNFTKVEN PVLIVEGKQA DNERLKAESD YLRGTIKDDL QDRMTGGFTS DNFQLIRTHG 

        70         80         90        100        110        120 
MYQQDDRDIR AERQKQKLEP LHNVMLRARL PGGIINPTQW LAIDKFADDY TSYGSIRLTT 

       130        140        150        160        170        180 
RQTFQFHGVL KPNIKLMHQT LNSVGLDSIA TAGDVNRNVL CTSNPVESAL HQEAYEWATK 

       190        200        210        220        230        240 
ISEHLLPKTR AYAEIWLDEE KVETTEADEI EPILGSNYLP RKFKTTVVIP PNNDIDVHAN 

       250        260        270        280        290        300 
DLNFVAISEG GELIGFNVLV GGGLAMTHGD KATYPRCADD FGFIPKEHTL AIAAAVVTTQ 

       310        320        330        340        350        360 
RDWGNRVNRK NAKTKYTLDR VGVDTFKAEV ERRAGIKFSE SRSYEFTHRG DSFGWVEGID 

       370        380        390        400        410        420 
GKNHLTLFIE NGRILDFNGA NSDSKALKTG MREIAKIHKG DFRLTANQNL IVAGVSAEDK 

       430        440        450        460        470        480 
TIIEQLAREH GLINDGVSNQ RKSSMACVAF PTCPLAMAEA ERYLPGLVDD VEAILEKNGL 

       490        500        510        520        530        540 
KDDSIILRVT GCPNGCGRAM LAEIGLVGKG PGKYNMYLGS DLAGSRVPKL YKENVDEAGV 

       550        560        570        580 
LSEIDALSAR WSAERNDGEA FGDFVIRAGI VEQVIVSFRD FHHA 

« Hide

References

[1]"The psychrophilic lifestyle as revealed by the genome sequence of Colwellia psychrerythraea 34H through genomic and proteomic analyses."
Methe B.A., Nelson K.E., Deming J.W., Momen B., Melamud E., Zhang X., Moult J., Madupu R., Nelson W.C., Dodson R.J., Brinkac L.M., Daugherty S.C., Durkin A.S., DeBoy R.T., Kolonay J.F., Sullivan S.A., Zhou L., Davidsen T.M. expand/collapse author list , Wu M., Huston A.L., Lewis M., Weaver B., Weidman J.F., Khouri H., Utterback T.R., Feldblyum T.V., Fraser C.M.
Proc. Natl. Acad. Sci. U.S.A. 102:10913-10918(2005) [PubMed: 16043709] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

CP000083 Genomic DNA. Translation: AAZ25063.1.
RefSeqYP_271404.1.

3D structure databases

ModBaseSearch...

Genome annotation databases

GeneID3518522.
GenomeReviewsGene locus CPS_4760 in contig CP000083_GR.
KEGGcps:CPS_4760.
NMPDRfig|167879.3.peg.4622.
TIGRCPS_4760.

Phylogenomic databases

HOGENOMQ47UW8.

Enzyme and pathway databases

BioCycCPSY167879:CPS_4760-MON.

Family and domain databases

HAMAPMF_01540.
[Tree]
InterProIPR011786. CysI.
IPR006066. Nir_Si_BS.
IPR006067. Nir_Sir_4Fe4S.
IPR005117. NiRdtase/SiRdtase_haem-b_fer.
[Graphical view]
PfamPF01077. NIR_SIR. 1 hit.
PF03460. NIR_SIR_ferr. 2 hits.
[Graphical view]
PRINTSPR00397. SIROHAEM.
TIGRFAMsTIGR02041. CysI. 1 hit.
PROSITEPS00365. NIR_SIR. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCYSI_COLP3
AccessionPrimary (citable) accession number: Q47UW8
Entry history
Integrated into UniProtKB/Swiss-Prot: June 26, 2007
Last sequence update: September 13, 2005
Last modified: November 25, 2008
This is version 20 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents