Q47TT4 (MDH_THEFY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Malate dehydrogenase
EC=1.1.1.37

Gene names

Name:	mdh
Ordered Locus Names:	Tfu_0092

Organism

Thermobifida fusca (strain YX) [Complete proteome] [HAMAP]

Taxonomic identifier

269800 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptosporangineae › Nocardiopsaceae › Thermobifida

Protein attributes

Sequence length	330 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reversible oxidation of malate to oxaloacetate By similarity. HAMAP MF_01517
Catalytic activity	(S)-malate + NAD+ = oxaloacetate + NADH. HAMAP MF_01517
Subunit structure	Homodimer By similarity. HAMAP MF_01517
Sequence similarities	Belongs to the LDH/MDH superfamily. MDH type 2 family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro malate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	L-malate dehydrogenase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 330	330	Malate dehydrogenase HAMAP MF_01517		PRO_0000113396
Regions
	Nucleotide binding	12 – 18	7	NAD By similarity
	Nucleotide binding	130 – 132	3	NAD By similarity
Sites
	Active site	188	1	Proton acceptor By similarity
	Binding site	93	1	Substrate By similarity
	Binding site	99	1	Substrate By similarity
	Binding site	106	1	NAD By similarity
	Binding site	113	1	NAD By similarity
	Binding site	132	1	Substrate By similarity
	Binding site	163	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q47TT4 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 9DC57DB6E5917012	FASTA	330	35,126
	10 20 30 40 50 60 MAKAPVNVTV TGAAGQIGYA LLFRIASGQL LGVDTPVRLR LLEIPQAIKA AEGTAMELDD 70 80 90 100 110 120 CAFPLLAGVD IYDDPRKAFD GVNVALLVGA RPRTKGMERR DLLEANGGIF GPQGAAINDG 130 140 150 160 170 180 AADDVRVLVV GNPANTNALI AQAHAPDIPA DRFTAMTRLD HNRALSQLAA KLNVSVSDIK 190 200 210 220 230 240 KLTIWGNHSA TQYPDIFHAE VNGRSAVEAV NDEEWLRDTF IPTVAKRGAA IIEARGASSA 250 260 270 280 290 300 ASAANAAIDH VYDWVNGTPE GDWTSVALPS DGSYGVPEGL VSSFPVVSRN GSWEIVQGLE 310 320 330 INEFSRERID ASVRELEEER EAVRALGLIK

References

[1]

"Genome sequence and analysis of the soil cellulolytic actinomycete Thermobifida fusca YX." Lykidis A., Mavromatis K., Ivanova N., Anderson I., Land M., DiBartolo G., Martinez M., Lapidus A., Lucas S., Copeland A., Richardson P., Wilson D.B., Kyrpides N. J. Bacteriol. 189:2477-2486(2007) [PubMed: 17209016] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: YX.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000088 Genomic DNA. Translation: AAZ54130.1.
RefSeq	YP_288153.1. NC_007333.1.
3D structure databases
ProteinModelPortal	Q47TT4.
SMR	Q47TT4. Positions 3-329.
ModBase	Search...
Protein-protein interaction databases
STRING	Q47TT4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3579671.
GenomeReviews	Gene locus Tfu_0092 in contig CP000088_GR.
KEGG	tfu:Tfu_0092.
NMPDR	fig|269800.4.peg.428.
PATRIC	23900568. VBITheFus33945_0095.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0039.
HOGENOM	HBG289884.
OMA	DPNVAFK.
ProtClustDB	PRK05442.
Enzyme and pathway databases
BioCyc	TFUS269800:TFU_0092-MONOMER.
Family and domain databases
HAMAP	MF_01517. Malate_dehydrog_2. [Tree]
InterPro	IPR001557. L-lactate/malate_DH. IPR022383. Lactate/malate_DH_C. IPR001236. Lactate/malate_DH_N. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR001252. Malate_DH_AS. IPR010945. Malate_DH_type2. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00024.
PANTHER	PTHR23382. MDH_SF1. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
SUPFAM	SSF56327. Lactate_DH/Glyco_hydro_4_C. 1 hit.
TIGRFAMs	TIGR01759. MalateDH-SF1. 1 hit.
PROSITE	PS00068. MDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MDH_THEFY

Accession

Primary (citable) accession number: Q47TT4

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	September 13, 2005
Last modified:	January 25, 2012
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program