Q47SX2 (ISPE_THEFY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
Short name=CMK
EC=2.7.1.148

Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Gene names

Name:	ispE
Ordered Locus Names:	Tfu_0407

Organism

Thermobifida fusca (strain YX) [Complete proteome] [HAMAP]

Taxonomic identifier

269800 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptosporangineae › Nocardiopsaceae › Thermobifida

Protein attributes

Sequence length	307 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP MF_00061
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 307	307	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061		PRO_0000235147
Regions
	Nucleotide binding	107 – 117	11	ATP Potential
Sites
	Active site	14	1	By similarity
	Active site	149	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q47SX2 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: DD48F4C68A0567B3	FASTA	307	31,099
	10 20 30 40 50 60 MNAPTSVTVR VPAKVNLRLA VGPVRGDGYH GLVNVFHAVS LYDEVTVSPR SDLAPGQAVL 70 80 90 100 110 120 SVDGERPDHV ARVPLDDSNL AARAAALLAR HVPHATGVHI HVHKAIPVAG GMAGGSADAA 130 140 150 160 170 180 AALVACDALW EGGTPRERLL ELAAELGSDV AFPLIGCTAV GTGRGEQLSP LPVQGTFHWV 190 200 210 220 230 240 FALVDGGLST TKVFAEYDRL RPDAPEPTLD DALVAALAAG DARRLGAALT NDLQPAALAL 250 260 270 280 290 300 RPELGDLLEA GRAAGALGAL VSGSGPTCAF LAESADHARE LAAALRASGT CADTVTAHGP VPGATVV

References

[1]

"Genome sequence and analysis of the soil cellulolytic actinomycete Thermobifida fusca YX." Lykidis A., Mavromatis K., Ivanova N., Anderson I., Land M., DiBartolo G., Martinez M., Lapidus A., Lucas S., Copeland A., Richardson P., Wilson D.B., Kyrpides N. J. Bacteriol. 189:2477-2486(2007) [PubMed: 17209016] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: YX.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000088 Genomic DNA. Translation: AAZ54445.1.
RefSeq	YP_288468.1. NC_007333.1.
3D structure databases
ProteinModelPortal	Q47SX2.
ModBase	Search...
Protein-protein interaction databases
STRING	Q47SX2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3580006.
GenomeReviews	Gene locus Tfu_0407 in contig CP000088_GR.
KEGG	tfu:Tfu_0407.
NMPDR	fig|269800.4.peg.21.
PATRIC	23901218. VBITheFus33945_0417.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1947.
HOGENOM	HBG734593.
OMA	KINLHLG.
PhylomeDB	Q47SX2.
ProtClustDB	PRK03188.
Enzyme and pathway databases
BioCyc	TFUS269800:TFU_0407-MONOMER.
Family and domain databases
HAMAP	MF_00061. IspE. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR004424. IspE. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00919.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00154. IspE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPE_THEFY

Accession

Primary (citable) accession number: Q47SX2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2006
Last sequence update:	September 13, 2005
Last modified:	January 25, 2012
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program