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Q47SA6

- SYE_THEFY

UniProt

Q47SA6 - SYE_THEFY

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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Thermobifida fusca (strain YX)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi106 – 1061ZincUniRule annotation
Metal bindingi108 – 1081ZincUniRule annotation
Metal bindingi128 – 1281ZincUniRule annotation
Metal bindingi130 – 1301ZincUniRule annotation
Binding sitei241 – 2411ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. glutamate-tRNA ligase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW
  4. tRNA binding Source: InterPro

GO - Biological processi

  1. glutamyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciTFUS269800:GI42-636-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:Tfu_0623
OrganismiThermobifida fusca (strain YX)
Taxonomic identifieri269800 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptosporangineaeNocardiopsaceaeThermobifida
ProteomesiUP000000434: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 477477Glutamate--tRNA ligasePRO_0000237415Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi269800.Tfu_0623.

Structurei

3D structure databases

ProteinModelPortaliQ47SA6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi12 – 2211"HIGH" regionAdd
BLAST
Motifi238 – 2425"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000252722.
KOiK01885.
OMAiKRDANIM.
OrthoDBiEOG6DRPF7.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q47SA6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTDKAIRTRF APSPTGMFHV GGARSALFNW ALALQQPEGK FVLRIEDTDA
60 70 80 90 100
ARNRPEWTEG ILRALSALGI DERDPHFEGP YFQSAYADKH REVAEELYRK
110 120 130 140 150
GRAYYCDCTR EQIQERRGNP HLGYDGYCRD RGLEPGPGRA LRFRVPEGGP
160 170 180 190 200
TVVDDKIRGR VEFDHSAIED FVIARSDGSP LFVLANVVDD VEMAITHVVR
210 220 230 240 250
GEEHLSNTPK QQLLWEALGQ TPPVWAHLPV IVNEKRQKLS KRRDKVALED
260 270 280 290 300
YLAEGYLPEA MVNYLMLLGW GPGDDREIMP FSEMVPLFRL EDVNSSSAFF
310 320 330 340 350
DEKKLRAFNG EYIRALDTTE FVERCAPWLG SEKAPWPAEN FDAQVFEAVA
360 370 380 390 400
PLAQTRISVL SEIVSYVDFL FLDRPVEDEK SWAKAMKPGV GEEMLTAALE
410 420 430 440 450
RFSDPNLEWR AEPLKAALEE VAAAQGLKLG KAQAPVRVAV TGRTVGLPLF
460 470
ESLELLGRSR VQERLRAALE KLKAAGE
Length:477
Mass (Da):53,668
Last modified:September 13, 2005 - v1
Checksum:i82327E88C794893F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000088 Genomic DNA. Translation: AAZ54661.1.
RefSeqiYP_288684.1. NC_007333.1.

Genome annotation databases

EnsemblBacteriaiAAZ54661; AAZ54661; Tfu_0623.
GeneIDi3580652.
KEGGitfu:Tfu_0623.
PATRICi23901684. VBITheFus33945_0647.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000088 Genomic DNA. Translation: AAZ54661.1 .
RefSeqi YP_288684.1. NC_007333.1.

3D structure databases

ProteinModelPortali Q47SA6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 269800.Tfu_0623.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAZ54661 ; AAZ54661 ; Tfu_0623 .
GeneIDi 3580652.
KEGGi tfu:Tfu_0623.
PATRICi 23901684. VBITheFus33945_0647.

Phylogenomic databases

eggNOGi COG0008.
HOGENOMi HOG000252722.
KOi K01885.
OMAi KRDANIM.
OrthoDBi EOG6DRPF7.

Enzyme and pathway databases

BioCyci TFUS269800:GI42-636-MONOMER.

Family and domain databases

Gene3Di 1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPi MF_00022_B. Glu_tRNA_synth_B.
InterProi IPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view ]
PANTHERi PTHR10119. PTHR10119. 1 hit.
Pfami PF00749. tRNA-synt_1c. 1 hit.
[Graphical view ]
PRINTSi PR00987. TRNASYNTHGLU.
SUPFAMi SSF48163. SSF48163. 1 hit.
TIGRFAMsi TIGR00464. gltX_bact. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: YX.

Entry informationi

Entry nameiSYE_THEFY
AccessioniPrimary (citable) accession number: Q47SA6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: September 13, 2005
Last modified: November 26, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3