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Protein

Trans,polycis-polyprenyl diphosphate synthase ((2Z,6E)-farnesyl diphosphate specific)

Gene

Tfu_0853

Organism
Thermobifida fusca (strain YX)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the synthesis of Z,E-mixed prenyl diphosphates by a condensation of isopentenyl diphosphate to an allylic diphosphate. It shows a large substrate specificity accepting dimethylallyl diphosphate (DMAPP), GPP, E,Efarnesyl diphosphate (FPP), E,E,E-geranylgeranyl diphosphate (GGPP), neryl diphosphate (Z-GPP), and (2Z,6E)-farnesyl diphosphate (Z,E-FPP) as allylic substrates. The enzyme exhibits the highest activity when Z,E-FPP is employed as an allylic substrate. The major product is dodecaprenyl diphosphate (C60) under every allylic substrate conditions, but the enzyme is also able to synthesize even C70 prenyl diphosphate as the maximum chain-length product.1 Publication

Catalytic activityi

(2Z,6E)-farnesyl diphosphate + n isopentenyl diphosphate = n diphosphate + trans,polycis-polyprenyl diphosphate (n = 9-11).1 Publication

Cofactori

Mg2+By similarityNote: Binds 2 magnesium ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei44By similarity1
Metal bindingi44MagnesiumBy similarity1
Binding sitei49SubstrateBy similarity1
Binding sitei57SubstrateBy similarity1
Binding sitei61SubstrateBy similarity1
Active sitei92Proton acceptorBy similarity1
Binding sitei93SubstrateBy similarity1
Binding sitei95SubstrateBy similarity1
Binding sitei212SubstrateBy similarity1
Metal bindingi231MagnesiumBy similarity1

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • prenyltransferase activity Source: UniProtKB

Keywordsi

Molecular functionTransferase
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-15682
TFUS269800:G1G4Q-868-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Trans,polycis-polyprenyl diphosphate synthase ((2Z,6E)-farnesyl diphosphate specific) (EC:2.5.1.88)
Alternative name(s):
Cis-prenyltransferase
Dodecaprenyl diphosphate synthase
Gene namesi
Ordered Locus Names:Tfu_0853
OrganismiThermobifida fusca (strain YX)
Taxonomic identifieri269800 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptosporangialesNocardiopsaceaeThermobifida
Proteomesi
  • UP000000434 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004191341 – 282Trans,polycis-polyprenyl diphosphate synthase ((2Z,6E)-farnesyl diphosphate specific)Add BLAST282

Proteomic databases

PRIDEiQ47RM6

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi269800.Tfu_0853

Structurei

3D structure databases

ProteinModelPortaliQ47RM6
SMRiQ47RM6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni45 – 48Substrate bindingBy similarity4
Regioni89 – 91Substrate bindingBy similarity3
Regioni218 – 220Substrate bindingBy similarity3

Sequence similaritiesi

Belongs to the UPP synthase family.Curated

Phylogenomic databases

eggNOGiENOG4105CR3 Bacteria
COG0020 LUCA
HOGENOMiHOG000006055
KOiK21273
OMAiFDRRDLW
OrthoDBiPOG091H00BZ

Family and domain databases

CDDicd00475 Cis_IPPS, 1 hit
Gene3Di3.40.1180.10, 1 hit
HAMAPiMF_01139 ISPT, 1 hit
InterProiView protein in InterPro
IPR001441 UPP_synth-like
IPR018520 UPP_synth-like_CS
IPR036424 UPP_synth-like_sf
PANTHERiPTHR10291 PTHR10291, 1 hit
PfamiView protein in Pfam
PF01255 Prenyltransf, 1 hit
SUPFAMiSSF64005 SSF64005, 1 hit
TIGRFAMsiTIGR00055 uppS, 1 hit
PROSITEiView protein in PROSITE
PS01066 UPP_SYNTHASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q47RM6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSPKTVFSTD THREPIPPQP HPSGARPPQL PRELIPRHVA IVMDGNGRWA
60 70 80 90 100
KQRGLPRTEG HKAGESSLFD VIEGALELGV PYLSAYAFST ENWKRSPDEV
110 120 130 140 150
RFLMGFNRDV IRRRRDELHA RGVRVRWAGR PGRLWKSVIK ELTEAEELTK
160 170 180 190 200
HNTKLTLQFC VNYGGRAEIA DAAAALARDV AAGRLSPNRV TEATLARYLY
210 220 230 240 250
HPDIPDVDLF IRSSGEQRLS NFLLWQSSYA EFVFLDTLWP DFDRRHFWQA
260 270 280
CEIYARRDRR YGGAEPNPVG PPQSAAGAQG QD
Length:282
Mass (Da):31,967
Last modified:September 13, 2005 - v1
Checksum:iA1085BD7CB076E90
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000088 Genomic DNA Translation: AAZ54891.1
RefSeqiWP_011291300.1, NC_007333.1

Genome annotation databases

EnsemblBacteriaiAAZ54891; AAZ54891; Tfu_0853
KEGGitfu:Tfu_0853

Similar proteinsi

Entry informationi

Entry nameiDPDP_THEFY
AccessioniPrimary (citable) accession number: Q47RM6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 5, 2012
Last sequence update: September 13, 2005
Last modified: May 23, 2018
This is version 81 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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