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Protein

Thiazole synthase

Gene

thiG

Organism
Thermobifida fusca (strain YX)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the rearrangement of 1-deoxy-D-xylulose 5-phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H2S.UniRule annotation

Catalytic activityi

1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-[sulfur-carrier protein ThiS] = 2-((2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O.UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei110Schiff-base intermediate with DXPUniRule annotation1
Binding sitei171DXP; via amide nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processThiamine biosynthesis
LigandSchiff base

Enzyme and pathway databases

BioCyciTFUS269800:G1G4Q-1062-MONOMER
UniPathwayiUPA00060

Names & Taxonomyi

Protein namesi
Recommended name:
Thiazole synthaseUniRule annotation (EC:2.8.1.10UniRule annotation)
Gene namesi
Name:thiGUniRule annotation
Ordered Locus Names:Tfu_1042
OrganismiThermobifida fusca (strain YX)
Taxonomic identifieri269800 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptosporangialesNocardiopsaceaeThermobifida
Proteomesi
  • UP000000434 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002363721 – 266Thiazole synthaseAdd BLAST266

Proteomic databases

PRIDEiQ47R37

Interactioni

Subunit structurei

Homotetramer. Forms heterodimers with either ThiH or ThiS.UniRule annotation

Protein-protein interaction databases

STRINGi269800.Tfu_1042

Structurei

3D structure databases

ProteinModelPortaliQ47R37
SMRiQ47R37
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni197 – 198DXP bindingUniRule annotation2
Regioni219 – 220DXP bindingUniRule annotation2

Sequence similaritiesi

Belongs to the ThiG family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CA8 Bacteria
COG2022 LUCA
HOGENOMiHOG000248049
KOiK03149
OMAiAQYPSPA
OrthoDBiPOG091H01K9

Family and domain databases

CDDicd04728 ThiG, 1 hit
Gene3Di3.20.20.70, 1 hit
HAMAPiMF_00443 ThiG, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR033983 Thiazole_synthase_ThiG
IPR008867 ThiG
PANTHERiPTHR34266 PTHR34266, 1 hit
PfamiView protein in Pfam
PF05690 ThiG, 1 hit

Sequencei

Sequence statusi: Complete.

Q47R37-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDAATGTTR ADTFEDPLVI AGEKFHSRLI MGTGGAPSLH VLADALRASG
60 70 80 90 100
TEMTTVAMRR VDPNAQGSVW DVLRETGVRP LPNTAGCFTA VDALRTARLG
110 120 130 140 150
REALETNWVK LEVVADERTL LPDPVETLDA AERLVDEGFV VLAYTNDDPV
160 170 180 190 200
LARRLAQVGC AAVMPLGAPI GSGMGIRNPH NIELIVEELD VPVILDAGIG
210 220 230 240 250
TASDAALAME LGCDAVLLAT AVTRAQDPVL MAHAMREAVS AGRRARLAGR
260
IPIRRYAHAS SPPREG
Length:266
Mass (Da):28,129
Last modified:September 13, 2005 - v1
Checksum:i42AE65A22DE4AD3D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000088 Genomic DNA Translation: AAZ55080.1
RefSeqiWP_011291489.1, NC_007333.1

Genome annotation databases

EnsemblBacteriaiAAZ55080; AAZ55080; Tfu_1042
KEGGitfu:Tfu_1042

Similar proteinsi

Entry informationi

Entry nameiTHIG_THEFY
AccessioniPrimary (citable) accession number: Q47R37
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: September 13, 2005
Last modified: May 23, 2018
This is version 85 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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